RESUMO
Early, conforming antibiotic treatment in elderly patients hospitalised for community-acquired pneumonia (CAP) is a key factor in the prognosis and mortality. The objective was to examine whether empirical antibiotic treatment was conforming according to the Spanish Society of Pulmonology and Thoracic Surgery guidelines in these patients. Multicentre study in patients aged ⩾65 years hospitalised due to CAP in the 2013-14 and 2014-15 influenza seasons. We collected socio-demographic information, comorbidities, influenza/pneumococcal vaccination history and antibiotics administered using a questionnaire and medical records. Bivariate analyses and multilevel logistic regression were made. In total, 1857 hospitalised patients were included, 82 of whom required intensive care unit (ICU) admission. Treatment was conforming in 51.4% (95% confidence interval (CI) 49.1-53.8%) of patients without ICU admission and was associated with absence of renal failure without haemodialysis (odds ratio (OR) 1.49, 95% CI 1.15-1.95) and no cognitive dysfunction (OR 1.71, 95% CI 1.25-2.35), when the effect of the autonomous community was controlled for. In patients with ICU admission, treatment was conforming in 45.1% (95% CI 34.1-56.1%) of patients and was associated with the hospital visits in the last year (<3 vs. ⩾3, OR 2.70, 95% CI 1.03-7.12) and there was some evidence that this was associated with season. Although the reference guidelines are national, wide variability between autonomous communities was found. In patients hospitalised due to CAP, health services should guarantee the administration of antibiotics in a consensual manner that is conforming according to clinical practice guidelines.
Assuntos
Antibacterianos/uso terapêutico , Infecções Comunitárias Adquiridas/tratamento farmacológico , Hospitalização/estatística & dados numéricos , Pneumonia/tratamento farmacológico , Guias de Prática Clínica como Assunto , Idoso , Idoso de 80 Anos ou mais , Feminino , Humanos , Unidades de Terapia Intensiva/estatística & dados numéricos , Modelos Logísticos , Masculino , EspanhaRESUMO
Despite the profound impact that skeletal muscle disorders may pose for the daily activities of wild terrestrial and marine mammals, such conditions have been rarely described in cetaceans. In this study, the authors aimed to determine the nature and prevalence of skeletal muscle lesions in small and large odontocetes and mysticetes ( n = 153) from 19 different species. A macroscopic evaluation of the epaxial muscle mass and a histologic examination of the longissimus dorsi muscle were performed in all cases. The only macroscopically evident change was variable degrees of atrophy of the epaxial muscles ( longissimus dorsi, multifidus, spinalis) in emaciated specimens. The histopathological study revealed single or combined morphological changes in 91.5% of the cases. These changes included the following: degenerative lesions (75.2%), muscle atrophy (37.9%), chronic myopathic changes (25.5%), parasitic infestation (9.2%), and myositis (1.9%). The skeletal muscle is easily sampled during a necropsy and provides essential microscopic information that reflects both local and systemic conditions. Thus, skeletal muscle should be systematically sampled, processed, and examined in all stranded cetaceans.
Assuntos
Cetáceos , Músculo Esquelético/patologia , Doenças Musculares/veterinária , Animais , Feminino , Masculino , Doenças Musculares/etiologia , Doenças Musculares/patologiaRESUMO
OBJECTIVE: To determine the epidemiological characteristics of significative bacteriuria (SB) and their relationship with sociodemographic factors and to analyze risk factors in inpatients. METHODS: Cross-sectional descriptive study carried out on urine culture samples received between 2016-2020 in the Microbiology laboratory, differentiating between minors and adults. The dependent variable was the presence of SB and the independent variables were age, sex, year, type of sample and source of the sample. In urine cultures of inpatients, risk factors were evaluated from the Minimum Basic Data Set. RESULTS: A total of 68,587 valid records (96.3% of the total) were analyzed. 40.8% (95% CI: 40.4%-41.2%) of urine cultures in adults and 33.8% (95% CI: 32.9%-34.7%) in children were positive, with an incidence that ranged in adults between 18.2 cases/1,000 inhabitants in 2016 and 14.6 cases/1,000 inhabitants in 2020 and 21.1 and 8.4 cases/1,000 inhabitants respectively in minors. Positive urine cultures were more frequent in children from urban areas compared to rural areas (OR=1.37; p<0.01). In hospitalized adults, for each year of age the risk of SB increased by 2%, it was 36% higher in women, 18% higher in obese patients and 17% more frequent in patients with kidney disease, (p<0.01). No relationship was observed between SB and diagnosis of COVID-19. CONCLUSIONS: The sociodemographic characteristics of the population with SB in our health area are similar to those found in other geographical areas worldwide, observing a decreasing trend in incidence in the years studied. The frequency of SB in children is higher in urban areas.
Assuntos
Bacteriúria , COVID-19 , Adulto , Bacteriúria/epidemiologia , Bacteriúria/microbiologia , Criança , Estudos Transversais , Feminino , Humanos , Incidência , Fatores de RiscoRESUMO
The aim of this work is to assess the evolution of caries and fluorosis prevalence and indices at 7 and 12 years of age in the Canary Islands through three cross epidemiological studies conducted in 1991, 1998 and 2006. The three studies followed a similar methodology, using the WHO diagnosis criteria and indications, except for the assessment of fluorosis, which was measured with the "Thylstrup and Fejerskov" index. The examining dentists were trained and calibrated in an area with high endemic fluorosis. The three studies took a sample of 1000 students for each age group. At 7 years of age, the decayed, and filled teeth (dft) varied from 1.42 in 1991 to 1.14 in 1998 and 1.37 in 2006. At 12 years of age, the Decayed, Missing, and Filled Teeth (DMFT) went from 1.86 to 1.21 and 1.51 in the three studies respectively and the significant caries index (SiC) went from 4.28 to 3.15 and 3.72. At this age, the Filled Rate rose from 24.05% in 1991 to 30.43% in 1998 and to 37.20% in 2006. In relation to fluorosis, at 12 years of age the percentage of healthy or fluorosis-free schoolchildren in the Canary Islands between the first and the second studies went down from 73% to 61.3% and went up to 78.5% in 2006, with a noticeable fall in the prevalence of children with severe and moderate fluorosis. Despite the fluctuations of caries shown in the three studies, at 12 years of age the Canary Islands have remained in the low level of caries of the WHO classification from 1991 up to now. The evolution in the prevalence of dental fluorosis shows the effect of the measures taken, 10 years after they were started.
Assuntos
Cárie Dentária/epidemiologia , Fluorose Dentária/epidemiologia , Criança , Feminino , Humanos , Masculino , Prevalência , Espanha/epidemiologiaRESUMO
In this work we have studied the morphology and evolution of Clara cells in the bronchiolar mucosa of lungs from 63 Swiss mice foetuses that were classified into three groups according to age (14, 16 and 18 days). A control group composed of 21 15-day-old Swiss mice was also studied. The most salient feature of the Clara cells observed was the occurrence of two types of secretory granules and a large smooth endoplasmic reticulum. On the other hand, the Clara cells of the control group had a single secretory granule. Clara cells thus seem to take part in bronchiolar metabolism, as they were quite abundant in the early foetal groups and diminished as birth approached. This cell decrease was confirmed by the control group (15-day-old mice), the bronchioles of which contained scant cells and numerous ciliated cells.
Assuntos
Brônquios/ultraestrutura , Animais , Brônquios/embriologia , Citoplasma/metabolismo , Citoplasma/ultraestrutura , Grânulos Citoplasmáticos/ultraestrutura , Retículo Endoplasmático/ultraestrutura , Feminino , Camundongos , Microscopia Eletrônica , Mucosa/citologia , Mucosa/ultraestrutura , Gravidez , Fixação de TecidosRESUMO
This paper report on the lesions occurred in the thymus in experimental acute African swine fever (ASF). Twenty-one pigs were inoculated with the highly virulent ASF virus (ASFV) isolate Spain-70. Animals were slaughtered from 1 to 7 days post infection (dpi). Three animals with similar features were used as controls. Thymus samples were fixed in 10% buffered formalin solution for histological and immunohistochemical study and in 2.5% glutaraldehyde for ultrastructural examination. For immunohistochemical study, the avidin-biotin-peroxidase complex (ABC) technique was used to demonstrate viral protein 73 and porcine myeloid-histiocyte antigen SWC3 using specific monoclonal antibodies. Cell apoptosis was evaluated by the TUNEL assay. Blood samples were taken daily from all pigs and were used for leukocyte counts. The results of this study show a severe thymocyte apoptosis not related to the direct action of ASFV on these cells, but probably to a quantitative increase in macrophages in the thymus and their activation. A decrease in the percentage of blood lymphocytes was observed at the same time No significant vascular changes were observed in the study. With these results we suggest that ASFV infection of the thymus does not seem to play a critical role in the acute disease. Although severe apoptosis was observed, animals died because of the severe lesions found in the other organs.
Assuntos
Vírus da Febre Suína Africana/patogenicidade , Febre Suína Africana/metabolismo , Febre Suína Africana/patologia , Apoptose , Timo/patologia , Febre Suína Africana/sangue , Vírus da Febre Suína Africana/ultraestrutura , Animais , Anticorpos Monoclonais/metabolismo , Contagem de Células/veterinária , Imuno-Histoquímica/veterinária , Leucócitos/metabolismo , Leucócitos/patologia , Leucopenia/veterinária , Ativação de Macrófagos , Macrófagos/metabolismo , Macrófagos/patologia , Macrófagos/ultraestrutura , Suínos , Timo/metabolismo , Timo/ultraestrutura , Fatores de Tempo , Proteínas Virais/metabolismo , VirulênciaRESUMO
Interpretation of changes in bone marrow during infectious processes is quite complex. This paper reports bone marrow lesions observed in pigs inoculated with a moderately virulent ASF virus strain and studies their relationship to the pathogenesis of the disease. In this work, we have carried out the structural and ultrastructural study of the bone marrow of 14 Large White x Landrace pigs that were inoculated by the intramuscular route with 10(5) 50% hemodsorbing doses (HAD50) of the Dominican Republic'78 ASF virus strain. The inoculated pigs were killed at 3, 5, 7, 9, 11, 13, 15 and 17 days postinjection. Analysis of cells and structures belonging to the two main bone-marrow compartments, the hematopoietic cells and the hematopoietic micro-environment, showed that after inoculation with a moderately virulent strain, the most significant changes occurred in macrophages and megakaryocytes, consisting in virus replication in these cell populations and apoptosis of megakaryocytes, related with the sudden and transitory thrombocytopenia detected in the subacute ASF.
Assuntos
Febre Suína Africana/patologia , Células da Medula Óssea/ultraestrutura , Medula Óssea/ultraestrutura , Febre Suína Africana/virologia , Animais , Medula Óssea/patologia , Medula Óssea/virologia , Células da Medula Óssea/patologia , Células da Medula Óssea/virologia , Hematopoese , Células-Tronco Hematopoéticas , Suínos , VirulênciaRESUMO
Fischer carbene complexes 1-7 are not ionized under standard electrospray ionization (ESI) conditions. We report here that unsaturated chromium and tungsten (Fischer) carbene complexes can be ionized in an electrospray ion source in the presence of electron-donor compounds such as hydroquinone (HQ) or tetrathiafulvalene (TTF). The addition of these compounds, which seem to act as electron transfer agents, permits the recording and study of their ESI mass spectra in the negative mode of detection. Both chromium and tungsten(0) carbene complexes undergo in the first fragmentation stage a double simultaneous decarbonylation process.
Assuntos
Elétrons , Metano/análogos & derivados , Metano/análise , Espectrometria de Massas por Ionização por Electrospray/métodos , Cromo/química , Compostos Heterocíclicos/química , Hidrocarbonetos , Hidroquinonas/química , Íons/química , Nitrilas/química , Cloreto de Tolônio/química , Tungstênio/químicaRESUMO
The glomerular alterations observed in a dog with acute spontaneous infectious canine hepatitis (ICH) are described. Histologic changes of the glomeruli were enlargement of the mesangium with presence of intranuclear inclusion bodies and without proliferation of mesangial cells. Electron microscopy revealed adenovirus replication sites in glomerular mesangial cells and in endothelial cells of glomerular capillaries, as well as a focal mesangial-sclerosing glomerulonephritis associated with electron dense deposits which were closely related with extracellular ICH viral particles and immunohistochemically reactive for immunoglobulin (Ig) G, IgA, IgM and C3c complement components.
Assuntos
Adenovirus Caninos/isolamento & purificação , Doenças do Cão/patologia , Glomerulosclerose Segmentar e Focal/patologia , Glomerulosclerose Segmentar e Focal/veterinária , Hepatite Infecciosa Canina/patologia , Hepatite Infecciosa Canina/virologia , Doença Aguda , Adenovirus Caninos/ultraestrutura , Animais , Doenças do Cão/metabolismo , Cães , Evolução Fatal , Mesângio Glomerular/patologia , Mesângio Glomerular/ultraestrutura , Glomerulosclerose Segmentar e Focal/virologia , Hepatite Infecciosa Canina/metabolismo , Imuno-Histoquímica , Masculino , Microscopia EletrônicaRESUMO
A commercially available polyclonal antibody and an avidin-biotin-peroxidase immunohistochemical technique were used to detect Brucella abortus antigens in formalin-fixed, paraffin-embedded tissues of lung and liver from 20 aborted bovine fetuses. Thirteen fetuses were obtained from farms with a previous history of brucellosis, and 7 were collected from farms without a history of brucellosis. Among the 13 aborted bovine fetuses obtained from farms with a history of brucellosis, immunoreactivity to B. abortus was detected in lung (9 fetuses) and in liver (1 fetus), whereas Brucella was cultured from abomasal contents in 9 fetuses (8 were immunohistochemically positive). In addition, 11 dams of these 13 aborted bovine fetuses had antibodies to Brucella. Brucella abortus was not detected by immunohistochemistry in the 7 aborted bovine fetuses collected from farms without a history of brucellosis. Bacteriologic culture and serologic tests were also negative for Brucella. The results of this study revealed that the immunohistochemical technique was sufficiently sensitive for detecting B. abortus antigens in formalin-fixed lung tissues from naturally aborted bovine fetuses. Although additional studies are necessary to rule out cross-reaction of the polyclonal antibody with other microorganisms that cause bovine abortion, this immunohistochemical technique could be a complementary tool to serology and bacteriology for the diagnosis of brucellosis.
Assuntos
Aborto Animal/microbiologia , Antígenos de Bactérias/análise , Brucella abortus/isolamento & purificação , Brucelose Bovina/diagnóstico , Animais , Anticorpos , Brucelose Bovina/embriologia , Bovinos , Feminino , Feto/microbiologia , Imuno-Histoquímica , Pulmão/microbiologia , Pulmão/patologia , Macrófagos/patologia , Gravidez , Estudos RetrospectivosRESUMO
Formalin-fixed, paraffin embedded tissues obtained from 40 pigs inoculated with a field isolate of hog cholera virus were examined for the presence of Gp55, a major structural protein of the virus envelope, using a monoclonal antibody-based immunohistochemical test with the avidin-biotin-peroxidase complex method. Immunoreactivity was detected in hog cholera virus-infected tissues but not in control pigs tissues, African swine fever virus-infected tissues, or bovine viral diarrhea virus-infected porcine or bovine tissues. The first positive reactions were seen in lymphatic tissues, digestive tract and skin on postinoculation day (pid) 4, respiratory and urinary tissues on pid 5, nervous tissues on pid 6, and endocrine tissues on pid 7. These staining reactions persisted until the last observation on pid 18. Hog cholera virus antigen was not detected in heart tissue at any time. The highest levels of antigen detection were found in tonsils, spleen, and pancreas, although the esophageal mucosa and skin epithelial cells were also intensely and widely stained. The cellular staining pattern of Gp55 had a ubiquitous distribution. It was found in epithelial cells, macrophages and circulating monocytes, endothelial cells, lymphoid cells, and glial cells. The results showed a high specificity and high sensitivity for detecting hog cholera Gp55 in formalin-fixed, paraffin embedded tissue samples. This method allows precise association of Gp55 with specific cells, tissues, and histologic lesions, making the technique suitable for use in routine diagnosis of hog cholera.
Assuntos
Vírus da Febre Suína Clássica/isolamento & purificação , Peste Suína Clássica/patologia , Proteínas do Envelope Viral/análise , Animais , Encéfalo/patologia , Encéfalo/virologia , Bovinos , Diagnóstico Diferencial , Sistema Digestório/patologia , Sistema Digestório/virologia , Imuno-Histoquímica/métodos , Tecido Linfoide/patologia , Tecido Linfoide/virologia , Valores de Referência , Sistema Respiratório/patologia , Sistema Respiratório/virologia , Pele/patologia , Pele/virologia , Suínos , Sistema Urinário/patologia , Sistema Urinário/virologiaRESUMO
Cryptosporidiosis in chickens from southern Spain is reported. Cryptosporidia were found in the trachea, esophagus, and epithelium of the bursa of Fabricius in chickens from different regions. In one flock, cryptosporidiosis was associated with respiratory problems and high mortality. In another flock, it was associated with low mortality and weight loss.
Assuntos
Galinhas/parasitologia , Criptosporidiose/epidemiologia , Doenças das Aves Domésticas/epidemiologia , Animais , Bolsa de Fabricius/parasitologia , Cryptosporidium/isolamento & purificação , Esôfago/parasitologia , Feminino , Espanha/epidemiologia , Traqueia/parasitologiaRESUMO
This paper describes an outbreak of hemorrhagic enteritis due to adenovirus in turkeys in Spain. Diagnosis of the disease was confirmed by histopathological examination and the observation of adenovirus in spleen mononuclear cells and intestinal infiltrate. Evidence was also found of intravascular coagulation, which may give rise to the bleeding considered characteristic of this disease.
Assuntos
Infecções por Adenoviridae/veterinária , Aviadenovirus/patogenicidade , Enterite/veterinária , Doenças das Aves Domésticas/etiologia , Perus , Infecções por Adenoviridae/epidemiologia , Infecções por Adenoviridae/etiologia , Animais , Aviadenovirus/isolamento & purificação , Aviadenovirus/ultraestrutura , Surtos de Doenças/veterinária , Enterite/epidemiologia , Hemorragia Gastrointestinal/epidemiologia , Hemorragia Gastrointestinal/etiologia , Hemorragia Gastrointestinal/veterinária , Corpos de Inclusão Viral/ultraestrutura , Intestinos/virologia , Microscopia Eletrônica , Doenças das Aves Domésticas/epidemiologia , Doenças das Aves Domésticas/patologia , Espanha/epidemiologia , Baço/virologiaRESUMO
An immunohistological study was carried out on lungs and livers of pigs experimentally infected with two different African swine fever virus (ASFV) isolates. ASFV antigen, swine immunoglobulins (IgM and IgG) and (Clq) complement were demonstrated in both organs at different stages of infection. The ASFV antigen was mainly found in mononuclear phagocytic system (MPS) cells. Immunoglobulins and complement were observed in plasma, infected and non-infected phagocytic cells and cell debris. These findings suggest the presence, in acute infection, of immune complexes which may be involved in immunopathogenic mechanisms.
Assuntos
Vírus da Febre Suína Africana/isolamento & purificação , Febre Suína Africana/microbiologia , Anticorpos Antivirais/análise , Antígenos Virais/análise , Complemento C1q/análise , Fígado/microbiologia , Pulmão/microbiologia , Febre Suína Africana/imunologia , Febre Suína Africana/patologia , Vírus da Febre Suína Africana/imunologia , Animais , Complexo Antígeno-Anticorpo/análise , Imunoglobulina G/análise , Imunoglobulina M/análise , Fígado/imunologia , Fígado/patologia , Pulmão/imunologia , Pulmão/patologia , Masculino , Suínos/imunologia , Suínos/microbiologiaRESUMO
Attention has already been drawn to the presence of pulmonary intravascular macrophages (PIMs) in species in which the lung plays an important part in the removal of particles from the bloodstream. The stimulation of PIMs under certain conditions has been related to pulmonary oedema and congestion. In order to ascertain whether these cells are found in rabbits, healthy animals were inoculated with a liver homogenate obtained from animals which had died of rabbit haemorrhagic disease, a condition characterized by vascular phenomena and pulmonary oedema. PIMs were detected both in experimental and healthy control animals. They shared the morphological characteristics reported for other species, particularly with respect to the presence of intercellular junctions with endothelial cells. In experimental animals, PIMs were actively involved in the removal of cell debris which may or may not have been associated with the presence of rabbit haemorrhagic disease virus antigen.
Assuntos
Hemorragia/veterinária , Macrófagos/patologia , Infecções por Parvoviridae/veterinária , Edema Pulmonar/veterinária , Coelhos , Animais , Hemorragia/patologia , Infecções por Parvoviridae/patologia , Alvéolos Pulmonares/patologia , Edema Pulmonar/patologiaRESUMO
The role of pulmonary intravascular macrophages (PIM) in a viral infection has been studied by structural and ultrastructural methods with two strains of the African swine fever (ASF) virus: the virulent strain E70 and the attenuated strain E75. Pulmonary intravascular macrophages were the cells of the mononuclear phagocyte system of the lung most involved in the replication of strain E70, showing a marked cytopathic effect and necrosis. Concurrently, their size and number increased sharply. This stimulation of PIMs by the virulent strain of the ASF virus, together with the cytopathic effect, might be the cause of the abundant masses of cell debris found in septal capillaries and thus be related to the pulmonary oedema that characterizes the acute forms of the disease. With the attenuated strain of the ASF virus, stimulation of PIMs, as well as viral replication within them, was also intense, but the cytopathic effect was less. Later, in the course of the infection, the number of PIMs decreased sharply and then alveolar macrophages increased, bearing most of the viral replication and signalling the onset of a pneumonic process.
Assuntos
Febre Suína Africana/patologia , Pneumopatias/veterinária , Macrófagos/patologia , Vírus da Febre Suína Africana/patogenicidade , Vírus da Febre Suína Africana/fisiologia , Animais , Efeito Citopatogênico Viral , Pneumopatias/patologia , Masculino , Microscopia Eletrônica , Suínos , Virulência , Replicação Viral/fisiologiaRESUMO
In this report the morphological and immunohistochemical features with respect to intermediate filament proteins and the neuroendocrine nature of bilateral neuroblastomas possibly originating from the adrenal medulla in a goat kid are presented. Histologically, the tumours were composed of small, round, blue cells organized in highly cellular nests and sheets separated by fibrovascular septa. Isolated cells mimicking the morphological features of neurones were observed in both tumours. Ultrastructurally, dense-core neurosecretory granules, about 100 nm in diameter, and irregularly organized neurotubular networks were seen. The small tumour cells were only labelled by vimentin, while the neurone-like cells were labelled by both neurone-specific enolase and synaptophysin. The lack of staining of the tumour cells by the neurofilament proteins antiserum, which was also observed in a human neuroblastoma (used as positive control) has been previously reported and is probably the result of the tissue processing and/or the poor differentiation stage of the tumours. The vimentin labelling of tumour cells could be explained by the poor differentiation stage of the tumours, since vimentin is the only intermediate filament protein in presumptive neuroblasts, being replaced by neurofilament proteins in later stages of neurogenesis.
Assuntos
Biomarcadores Tumorais/análise , Doenças das Cabras/patologia , Proteínas de Filamentos Intermediários/análise , Proteínas de Neoplasias/análise , Neoplasias Primárias Múltiplas/veterinária , Neuroblastoma/veterinária , Proteínas de Neurofilamentos/análise , Neoplasias Retroperitoneais/veterinária , Animais , Grânulos Citoplasmáticos/química , Grânulos Citoplasmáticos/ultraestrutura , Doenças das Cabras/metabolismo , Cabras , Neoplasias Primárias Múltiplas/química , Neoplasias Primárias Múltiplas/patologia , Neuroblastoma/química , Neuroblastoma/patologia , Fosfopiruvato Hidratase/análise , Neoplasias Retroperitoneais/química , Neoplasias Retroperitoneais/patologia , Sinaptofisina/análise , Vimentina/análiseRESUMO
Thirty pigs were inoculated with a virulent isolate (Quillota strain) of classical swine fever (hog cholera) virus to establish the chronological occurrence of lesions in the kidney and to determine the mechanism responsible for renal haemorrhages. The study included the use of histopathological, ultrastructural, immunohistochemical (detection of viral antigen gp55, MAC387, lambda chains, CD3 and C1q) and morphometrical techniques (vascular area). Renal interstitial oedema and haemorrhages were detected from 7 days post-inoculation (dpi), associated with a slight interstitial mononuclear infiltrate and evidence of viral infection in macrophages and fibroblasts, and in a small proportion of lymphocytes. Viral infection was not detected in capillary endothelial cells. An intense mononuclear infiltrate, with B cells, T cells and small numbers of macrophages, was detected from 10 dpi. In the final phase of the experiment (14 dpi), slight proliferation and degranulation of mast cells were observed. Increased expression of the C1q component of complement was also detected. A significant increase in vascular area was observed from 7 dpi. These results suggest that haemorrhages observed in the kidneys of pigs inoculated with the Quillota strain resulted from erythrodiapedesis and increased vascular permeability, probably aggravated by mast cell degranulation in the final stage of the experiment. The results suggested that mast cell degranulation was linked to activation of the complement system.
Assuntos
Movimento Celular/fisiologia , Vírus da Febre Suína Clássica , Peste Suína Clássica/etiologia , Eritrócitos/fisiologia , Hemorragia/etiologia , Nefropatias/etiologia , Animais , Antígenos Virais/análise , Biomarcadores/análise , Capilares/metabolismo , Capilares/ultraestrutura , Capilares/virologia , Fragilidade Capilar/fisiologia , Permeabilidade Capilar/fisiologia , Degranulação Celular/fisiologia , Peste Suína Clássica/imunologia , Peste Suína Clássica/patologia , Vírus da Febre Suína Clássica/imunologia , Vesículas Citoplasmáticas/fisiologia , Vesículas Citoplasmáticas/ultraestrutura , Edema/etiologia , Edema/patologia , Endotélio Vascular/metabolismo , Endotélio Vascular/ultraestrutura , Endotélio Vascular/virologia , Eritrócitos/citologia , Feminino , Hemorragia/patologia , Nefropatias/patologia , Masculino , Mastócitos/fisiologia , Mastócitos/ultraestrutura , SuínosRESUMO
The pathological effect of haemorrhagic fever viruses on the kidney have not been clearly documented. This study reports glomerular lesions in African swine fever. In the acute form of the disease there was an acute diffuse proliferative glomerulonephritis, which was believed to be related to virus replication in circulating monocytes and glomerular mesangial cells, and to the presence of abundant circulating cell debris resulting from viral replication at other sites. In the subacute form, the proliferative mesangial glomerulonephritis observed may have been associated with systemic immune-mediated phenomena, and with subendothelial and mesangial deposits of immunoglobulins and complement components.
Assuntos
Febre Suína Africana/patologia , Glomerulonefrite Membranoproliferativa/patologia , Glomérulos Renais/patologia , Febre Suína Africana/imunologia , Animais , Feminino , Glomerulonefrite Membranoproliferativa/imunologia , Imuno-Histoquímica , Glomérulos Renais/imunologia , Glomérulos Renais/virologia , Masculino , Microscopia Eletrônica , Suínos , Fatores de TempoRESUMO
To study the effect of hog cholera virus on the epithelial cells of the bronchiolar mucosa, 12 pigs were inoculated with a highly virulent strain. Immunohistochemical and ultrastructural examination of the ciliated epithelial cells demonstrated an increase in the number of atypical cilia. The latter showed alterations in the microtubular pattern, possibly resulting from viral interference with the normal metabolism of the epithelial cells.