Neutrophil virucidal activity against SARS-CoV-2 is mediated by NETs.

BACKGROUND: The inflammation in the lungs and other vital organs in COVID-19 are characterized by the presence of neutrophils and high concentration of neutrophil extracellular traps (NETs), which also seems to mediate host tissue damage. However, it is not known whether NETs could have virucidal activity against SARS-CoV-2. METHODS: We investigated whether NETs could prevent SARS-CoV-2 replication in neutrophils and epithelial cells, and what the consequence of NETs degradation in K18-humanized ACE2 transgenic mice infected with SARS-CoV-2. RESULTS: Here, by immunofluorescence microscopy we observed that viral particles co-localize with NETs in neutrophils isolated from COVID-19 patients or from healthy individuals and infected in vitro. The inhibition of NETs production increased virus replication in neutrophils. In parallel, we observed that NETs inhibited virus abilities to infect and replicate in epithelial cells after 24 h of infection. Degradation of NETs with DNase I prevented their virucidal effect in vitro. Using K18-humanized ACE2 transgenic mice we observed a higher viral load in animals treated with DNase I. On the other hand, the virucidal effect of NETs was not dependent on neutrophil elastase or myeloperoxidase activity. CONCLUSION: Our results provide evidence of the role of NETosis as a mechanism of SARS-CoV-2 viral capture and inhibition.

Comparison of senescence phenotype of short- and long- term cultured rat mesenchymal stem cells in vitro.

Mesenchymal stem cells present clinical potential to recover and regenerate injured tissues in diverse pathologies. The in vitro expansion and characterization of these cells contribute to elucidation of the mechanisms of senescence and strategies involving cell therapies. This study aimed to compare specific characteristics between initial and advanced passages of mesenchymal stem cells derived from adipose tissue and bone marrow. Both cell types were characterized according to immunophenotype, osteogenic differentiation, genomic instability, migration assay, doubling population time and colony forming ability. Our results demonstrated that both cell types were able to maintain an immunophenotypic profile typical of mesenchymal stem cells during increasing passages. Adipose stem cells at initial passage presented greater migration capacity compared to advanced passage cells, and advanced passage cells proliferated faster than initial passage cells. Bone marrow stem cells at early passages presented higher osteogenic potential than advanced. At advanced passages they presented higher colony forming capacity and genetic damage than those at initial passage. These results suggest that mesenchymal stem cells maintained in culture presented characteristics of senescence that should be monitored prior the use in regenerative medicine and cells derived from bone marrow at initial passage have better potential for therapeutic use in bone tissue engineering.

Fibromyalgia: A Review of Related Polymorphisms and Clinical Relevance.

Fibromyalgia (FM) is a chronic pain syndrome that affects the central nervous system and generates disability, which is characterized by generalized pain, fatigue, and functional decline. In this review, we aimed to identify the polymorphisms related to the pathophysiology of FM and the clinical characteristics generated by genetic influence. Only original studies with genes related to FM were considered, totaling 27 articles. The genes found were: MTHFR, RGS4, MYT1L, TACR1, SCN9A, DRD3, ADRB2, IL-4, HLA-DRB1, EDN1, CNR1, TAAR1, OPRM1, ADRA1A, ADRB3, BDNF, GRIA4, HTR3A, HTR3B, HTR2A, SERPINA 1 or A1AT, NRXN3, GCH1, MEFV, TRPV3, SLC6A4, ACE I/D, TSPO, COMT, and MAOA. Several genes related to different pain syndromes and altered pain thresholds have been identified and some polymorphisms were related to susceptibility to FM. It was observed that 73.33% of the genes related to FM were also associated with some psychological disorders, such as anxiety, depression, schizophrenia, and obsessive and compulsive disorder, and 40.00% with pain sensitivity and/or migraine, besides other disorders associated (drug addiction, autoimmune disorders, circulatory problems, and metabolic alterations). This review demonstrated an association of FM and genetic polymorphisms that can expand our knowledge about the pathophysiology of this disease.

Cytotoxic and genotoxic evaluation of cotinine using human neuroblastoma cells (SH-SY5Y).

Cotinine is the main metabolite of nicotine, which is metabolized in the liver through a cytochrome P450 enzyme. Different studies point to genetic instability caused by nicotine, such as single and double DNA strand breaks and micronuclei formation, but little is known about the effect of cotinine. Therefore, the present in vitro study assessed the effects of cotinine on cell viability and DNA damage in SH-SY5Y neuroblastoma cells, as well as genotoxicity related to oxidative stress mechanisms. Comparisons with nicotine were also performed. An alkaline comet assay modified by repair endonucleases (FPG, OGG1, and Endo III) was used to detect oxidized nucleobases. SH-SY5Y neuronal cells were cultured under standard conditions and exposed for 3 h to different concentrations of cotinine and nicotine. Cytotoxicity was observed at higher doses of cotinine and nicotine in the MTT assay. In the trypan blue assay, cells showed viability above 80% for both compounds. Alkaline comet assay results demonstrated a significant increase in damage index and frequency for cells treated with cotinine and nicotine, presenting genotoxicity. The results of the enzyme-modified comet assay suggest a DNA oxidative damage induced by nicotine. Unlike other studies, our results demonstrated genotoxicity induced by both cotinine and nicotine. The similar effects observed for these two pyridine alkaloids may be due to the similarity of their structures.

Systems chemo-biology analysis of DNA damage response and cell cycle effects induced by coal exposure.

Cell cycle alterations are among the principle hallmarks of cancer. Consequently, the study of cell cycle regulators has emerged as an important topic in cancer research, particularly in relation to environmental exposure. Particulate matter and coal dust around coal mines have the potential to induce cell cycle alterations. Therefore, in the present study, we performed chemical analyses to identify the main compounds present in two mineral coal samples from Colombian mines and performed systems chemo-biology analysis to elucidate the interactions between these chemical compounds and proteins associated with the cell cycle. Our results highlight the role of oxidative stress generated by the exposure to the residues of coal extraction, such as major inorganic oxides (MIOs), inorganic elements (IEs) and polycyclic aromatic hydrocarbons (PAH) on DNA damage and alterations in the progression of the cell cycle (blockage and/or delay), as well as structural dysfunction in several proteins. In particular, IEs such as Cr, Ni, and S and PAHs such as benzo[a]pyrene may have influential roles in the regulation of the cell cycle through DNA damage and oxidative stress. In this process, cyclins, cyclin-dependent kinases, zinc finger proteins such as TP53, and protein kinases may play a central role.

Isolation and characterization of mesenchymal stem/stromal cells from Ctenomys minutus.

Mesenchymal stem/stromal cells (MSCs) are multipotent cells distributed in all tissues and characterized by adherence, morphology, immunophenotype and trilineage differentiation potential. The present study aimed to isolate and characterize adherent MSC-like populations from different tissues of Ctenomys minutus, a threatened wildlife rodent popularly known as tuco-tuco. Adherent cells were isolated from bone marrow, brain, liver, pancreas and adipose tissue of three adult animals collect in southern Brazil. Cultures showed typical morphology and proliferation potential. Adipose-derived MSCs showed trilineage potential. Cultures derived from adipose tissue, bone marrow and brain were immunophenotyped with negative results for CD31, CD44, CD45, CD106, and MHC class II, as well as strong positive results for CD29. Low fluorescence levels were seen for CD49d, CD90.2 and CD117. Cultures were negative for CD49e, except for brain-derived cultures that were weakly positive. CD11b was negative in adipose-derived MSCs, but positive in brain and bone marrow-derived cultures. The scratch assay showed high migration potential for pancreas and adipose tissue-derived cells. This study represents the first report of isolation and characterization of cultures having characteristics of MSCs from Ctenomys minutus. The collection of biological information for biobanks represents an important contribution to the creation of strategies for prevention of loss of genetic diversity.

Protective effects of acerola juice on genotoxicity induced by iron in vivo.

Metal ions such as iron can induce DNA damage by inducing reactive oxygen species (ROS) and oxidative stress. Vitamin C is one of the most widely consumed antioxidants worldwide, present in many fruits and vegetables, especially inMalpighia glabra L., popularly known as acerola, native to Brazil. Acerola is considered a functional fruit due to its high antioxidant properties and phenolic contents, and therefore is consumed to prevent diseases or as adjuvant in treatment strategies. Here, the influence of ripe and unripe acerola juices on iron genotoxicity was analyzed in vivo using the comet assay and micronucleus test. The comet assay results showed that acerola juice exerted no genotoxic or antigenotoxic activity. Neither ripe nor unripe acerola juices were mutagenic to animals treated with juices, in micronucleus test. However, when compared to iron group, the pre-treatment with acerola juices exerted antimutagenic activity, decreasing significantly micronucleus mean values in bone marrow. Stage of ripeness did not influence the interaction of acerola compounds with DNA, and both ripe and unripe acerola juices exerted protective effect over DNA damage generated by iron.

DNA damage in children and adolescents with cardiovascular disease risk factors.

The risk of developing cardiovascular disease (CVD) is related to lifestyle (e.g. diet, physical activity and smoking) as well as to genetic factors. This study aimed at evaluating the association between CVD risk factors and DNA damage levels in children and adolescents. Anthropometry, diet and serum CVD risk factors were evaluated by standard procedures. DNA damage levels were accessed by the comet assay (Single cell gel electrophoresis; SCGE) and cytokinesis-blocked micronucleus (CBMN) assays in leukocytes. A total of 34 children and adolescents selected from a population sample were divided into three groups according to their level of CVD risk. Moderate and high CVD risk subjects showed significantly higher body fat and serum CVD risk markers than low risk subjects (P<0.05). High risk subjects also showed a significant increase in DNA damage, which was higher than that provided by low and moderate risk subjects according to SCGE, but not according to the CBMN assay. Vitamin C intake was inversely correlated with DNA damage by SCGE, and micronucleus (MN) was inversely correlated with folate intake. The present results indicate an increase in DNA damage that may be a consequence of oxidative stress in young individuals with risk factors for CVD, indicating that the DNA damage level can aid in evaluating the risk of CVD.

Neutrophil and Eosinophil DNA Extracellular Trap Formation: Lessons From Pathogenic Fungi.

Fungal infections represent a worldwide health problem. Fungal pathogens are responsible for a variety of conditions, including superficial diseases, allergic pathologies and potentially lethal invasive infections. Neutrophils and eosinophils have been implicated as effector cells in several pathologies. Neutrophils are major effector cells involved in the control of fungal infections and exhibit a plethora of antifungal mechanisms, such as phagocytosis, reactive oxygen species production, degranulation, extracellular vesicle formation, and DNA extracellular trap (ET) release. Eosinophils are polymorphonuclear cells classically implicated as effector cells in the pathogenesis of allergic diseases and helminthic infections, although their roles as immunomodulatory players in both innate and adaptive immunity are currently recognized. Eosinophils are also endowed with antifungal activities and are abundantly found in allergic conditions associated with fungal colonization and sensitization. Neutrophils and eosinophils have been demonstrated to release their nuclear and mitochondrial DNA in response to many pathogens and pro-inflammatory stimuli. ETs have been implicated in the killing and control of many pathogens, as well as in promoting inflammation and tissue damage. The formation of ETs by neutrophils and eosinophils has been described in response to pathogenic fungi. Here, we provide an overview of the mechanisms involved in the release of neutrophil and eosinophil ETs in response to fungal pathogens. General implications for understanding the formation of ETs and the roles of ETs in fungal infections are discussed.

Selection for Growth and Precocity Alters Muscle Metabolism in Nellore Cattle.

To clarify the relationship between beef genetic selection for growth and precocity with muscle metabolism and metabolites, we performed metabolomic analysis using Longissimus lumborum (LL) muscle from Nellore cattle with divergent selection for these traits (high growth, HG; low growth, LG; high precocity, HP; low precocity, LP). Genetic potential for growth affected muscle protein and energetic metabolism. HG animals had a high concentration of arginine, carnosine, and leucine compared to LG animals. HP animals presented a high concentration of glutamine, betaine, creatinine, isoleucine, carnitine, acetyl carnitine, and lower levels of glucose compared to LP animals, affecting protein and fatty acid metabolism. Intensity of selection (high or low) was correlated with changes in protein metabolism, and the type of selection (growth or precocity) affected fat metabolism. In conclusion, both HG and HP appear to be correlated with a high concentration of protein metabolites and changes in protein metabolic pathways, while selection for precocity is more correlated with changes in fat metabolism compared to animals selected for growth.

[Insufficient levels of physical activity of adolescents associated with sociodemographic, environmental and school factors]. / Níveis insuficientes de atividade física de adolescentes associados a fatores sociodemográficos, ambientais e escolares.

This study investigated the associations between the level of physical activity with sociodemographic, environmental and academic factors of adolescents. Two thousand five hundred and forty-five adolescents aged between 14 and 18 from Florianópolis and neighboring cities participated in this study. The short version of the International Physical Activity Questionnaire (IPAQ) was used. The prevalence of inactive adolescents was 48.6%, being higher among females (53.9%). For girls, physical inactivity was associated with the lack of appropriate and free places for physical activity (OR = 1.30 95% CI 1.05 to 1.82), with no influence of other variables. For boys who had no professional activity (OR = 1.54 95% CI 1.14 to 2.08), less educated mothers (OR = 0.41 95% CI 0.27 to 0.75), or graduate mothers (OR = 0.35 95% CI 0,17- 0,73), living in major cities (OR = 1.64 95% CI 1.17 to 2.31) and low socioeconomic classification when compared to the average (OR = 0.69 95% CI 0.49 to 0.97) were more prone to physical inactivity. It was observed that the socio-demographic variables were associated with the inactive behavior of boys and environmental variables were associated with the physical inactivity of girls. Among the academic factors, only having to repeat a year was associated with inactivity, and only for girls.

Este estudo investigou as associações entre o nível de atividade física com os fatores sociodemográficos, ambientais e escolares de adolescentes. Participaram 2.545 jovens de 14 a 18 anos dos municípios da Grande Florianópolis. Foi utilizada a versão curta do Questionário Internacional de Atividade Física (IPAQ). A prevalência de jovens inativos foi de 48,6%, sendo maior no sexo feminino (53,9%). Para as moças, a inatividade física esteve associada com a falta de lugares apropriados e gratuitos para a prática de atividade física (OR = 1,30 IC95%1,05-1,82), independente da influência das outras variáveis. Já para os rapazes, aqueles que não possuíam atividade profissional (OR = 1,54 IC95%1,14-2,08), mães de menor instrução (OR = 0,41 IC95% 0,27-0,75) ou mães com ensino superior (OR = 0,35 IC95% 0,17- 0,73), pertencentes a cidades de grande porte (OR = 1,64 IC95%1,17-2,31) e classificação socioeconômica baixa quando comparada à média (OR = 0,69 IC95% 0,49-0,97), apresentaram maior chance de inatividade física. Observou-se que as variáveis sociodemográficas estiveram associadas ao comportamento inativo dos rapazes e às variáveis ambientais se associaram a inatividade física das moças. Já dentre os fatores escolares, apenas a reprovação escolar foi associada com inatividade, e somente para as moças.

Evaluation of genotoxicity and toxicity of water and sediment samples from a Brazilian stream influenced by tannery industries.

This paper reports results of genotoxicity and toxicity studies of water and sediment samples collected from the Estância Velha stream of southern Brazil, a stream transporting both domestic sewage and effluents from regional factories working in the leather industry. Three sites were selected: in the stream headwaters (Site 1), located downstream of an urban area (Site 2), and near the basin outfall (Site 3). Results obtained with Allium cepa showed no evidence of chromosomal mutation, either in water or in sediment, during winter or summer seasons, but samples collected below Site 1 showed high toxicity. Physical and chemical analyses showed high concentrations of pollutants at these sites. Ecotoxicity tests with Daphnia magna and Ceriodaphnia dubia measured toxicity in water from Sites 2 and 3 in summer 2004. A toxic effect on Hyalella azteca was only found in sediment from Site 3 during winter 2003 and summer 2004. The results suggest that the synergy among different compounds in domestic and industrial sewage discharges can make it difficult to maintain system stability.

Fish as bioindicators to assess the effects of pollution in two southern Brazilian rivers using the Comet assay and micronucleus test.

Industrial effluents, agricultural runoff, and municipal wastewaters contain unknown substances and complex mixtures that are released into the environment and can lead to contamination of surface and subsurface waters. In the present report, we have used the alkaline Comet assay and the micronucleus (MN) test to detect the genotoxicity due to multiple sources of pollution in the peripheral blood of two native estuarine fish (mullet and sea catfish) and evaluated possible interactive genotoxic effects from multiple contaminants and the seasonal variation of the genotoxicity. Mullet and sea catfish were captured in the Tramandai and Mampituba Rivers in the southern Brazilian state of Rio Grande do Sul. Reference animals were obtained from the Armazem lagoon. Fish captured in the two estuaries during the four seasons over a period of 2 years had increased levels of DNA damage and MN frequencies relative to the reference fish. In general, the alkaline Comet assay was more sensitive to the genotoxicity of the river contaminants than the MN test. The Comet assay demonstrated significant differences in fish captured at different seasons and at the two river sites, while the MN test showed significant differences only for the annual average for mullet from both sites and fish from the control site. The increases in DNA damage appear to be related to the increase in the number of people in the towns close to the study areas during the warm spring and summer seasons. Although no specific cause-effect relationships were established, comparison of the chemical contaminants and physical variations in the rivers with the genotoxicity data indicate that there may be some association between hydrocarbons, metals, pH, and water temperature and the level of damaged cells observed in mullet and sea catfish from the Tramandai and Mampituba estuaries.

Níveis insuficientes de atividade física de adolescentes associados a fatores sociodemográficos, ambientais e escolares / Insufficient levels of physical activity of adolescents associated with sociodemographic, environmental and school factors

Resumo Este estudo investigou as associações entre o nível de atividade física com os fatores sociodemográficos, ambientais e escolares de adolescentes. Participaram 2.545 jovens de 14 a 18 anos dos municípios da Grande Florianópolis. Foi utilizada a versão curta do Questionário Internacional de Atividade Física (IPAQ). A prevalência de jovens inativos foi de 48,6%, sendo maior no sexo feminino (53,9%). Para as moças, a inatividade física esteve associada com a falta de lugares apropriados e gratuitos para a prática de atividade física (OR = 1,30 IC95%1,05-1,82), independente da influência das outras variáveis. Já para os rapazes, aqueles que não possuíam atividade profissional (OR = 1,54 IC95%1,14-2,08), mães de menor instrução (OR = 0,41 IC95% 0,27-0,75) ou mães com ensino superior (OR = 0,35 IC95% 0,17- 0,73), pertencentes a cidades de grande porte (OR = 1,64 IC95%1,17-2,31) e classificação socioeconômica baixa quando comparada à média (OR = 0,69 IC95% 0,49-0,97), apresentaram maior chance de inatividade física. Observou-se que as variáveis sociodemográficas estiveram associadas ao comportamento inativo dos rapazes e às variáveis ambientais se associaram a inatividade física das moças. Já dentre os fatores escolares, apenas a reprovação escolar foi associada com inatividade, e somente para as moças.

Abstract This study investigated the associations between the level of physical activity with sociodemographic, environmental and academic factors of adolescents. Two thousand five hundred and forty-five adolescents aged between 14 and 18 from Florianópolis and neighboring cities participated in this study. The short version of the International Physical Activity Questionnaire (IPAQ) was used. The prevalence of inactive adolescents was 48.6%, being higher among females (53.9%). For girls, physical inactivity was associated with the lack of appropriate and free places for physical activity (OR = 1.30 95% CI 1.05 to 1.82), with no influence of other variables. For boys who had no professional activity (OR = 1.54 95% CI 1.14 to 2.08), less educated mothers (OR = 0.41 95% CI 0.27 to 0.75), or graduate mothers (OR = 0.35 95% CI 0,17- 0,73), living in major cities (OR = 1.64 95% CI 1.17 to 2.31) and low socioeconomic classification when compared to the average (OR = 0.69 95% CI 0.49 to 0.97) were more prone to physical inactivity. It was observed that the socio-demographic variables were associated with the inactive behavior of boys and environmental variables were associated with the physical inactivity of girls. Among the academic factors, only having to repeat a year was associated with inactivity, and only for girls.

Perfil dos Idosos do Centro-dia para Idosos "A Mão Branca" / Profile of the Senior Citizens of the Center Day for the Elderly "The White Hand" / Perfil de los ancianos del centro-día para ancianos "La mano blanca"

Em junho de 2016, a Associação Beneficente "A Mão Branca" de Amparo aos Idosos inaugurou um Centro-dia com a finalidade de atender idosos parcialmente dependentes. Este artigo tem o objetivo de apresentar indicadores relativos aos idosos beneficiários desse serviço, de junho de 2016 a setembro de 2017. Passaram pelo Centro 25 idosos com idade média de 84,3 anos, dois quais 67% são do sexo feminino. A principal procura pelo serviço foi pelos familiares, sendo que os motivos que os levaram a buscar o Centro-dia foram a ociosidade e a redução do convívio social com 72% dos casos, seguidos de 65% dos que apresentaram queixas relacionadas à memória. Fazemse necessários novos estudos voltados a esta nova modalidade do cuidar.

In June 2016, the Associação Beneficente "A Mão Branca" de Amparo aos Idosos has inaugurated a Day Care aiming the partially dependent elderly. This article has the object goal to present the indicators related to the elderly beneficiaries of this servisse from June 2016 to September 2017. Twenty-four seniors 84 years old on average, were enrolled in the service, 67% of them were female. The main demand for the service comes from the elderly 's family, whose concerns about their senior relatives idleness and lack of social interaction, urge them to look for our support expertise. Complaints related to the elderly memory are also strong reasons for the increase of our service demand. Further studies are necessary on this new modality of caring.

En junio de 2016, la Asociación Beneficente "La Mano Blanca" de Amparo a los Ancianos inauguró un Centro-día con la finalidad de atender a ancianos parcialmente dependientes. Este artículo tiene el objetivo de presentar los indicadores relativos a los ancianos beneficiários de esse sevicio, de junho de 2016 a septiembre de 2017. Pasaron por el servicio a 25 ancianos con edad promedio de 84,3 años, dos que el 67% son del sexo femenino. La principal demanda por el servicio fue por los familiares, siendo que los motivos que los llevaron a buscar el Centro-día fueron la ociosidad y la reducción de la convivencia social con el 72% de los casos, seguidos del 65% de los que presentaron quejas relacionadas a la memoria. Se hacen necesarios nuevos estudios orientados a esta nueva modalidad del cuidar.

A fome e o Direito Humano à Alimentação Adequada (DHAA) em filmes documentários brasileiros / Hunger and the Human Right to Adequate Nutrition (HRAN) in Brazilian documentary films

INTRODUÇÃO: A fome e a desnutrição deixaram de ser temas de destaque nos meios midiáticos contemporâneos. No entanto, muitas discussões ainda podem ser levantadas sobre estas questões que ainda persistem nos dias atuais de muitos brasileiros. OBJETIVO: Analisar como o Direito Humano a Alimentação Adequada a partir do conteúdo abordado em documentários e matérias jornalísticas nacionais sobre a fome. MATERIAIS E MÉTODOS: O estudo utilizou de métodos audiovisuais a fim de realizar um recorte analítico-interpretativo. É de natureza qualitativa numa perspectiva de análise documental, abordando com maior profundidade uma realidade social denunciada em documentários de televisão e matérias jornalísticas. RESULTADOS. Os materiais analisados possuem em comum é a abordagem de temáticas delicadas e que foram abordadas de modo a provocarem incômodo na audiência. “Garapa” é um filme em tom de denúncia social, retratado de forma incomum para produções contemporâneas, mostrando a desordem social instalada naquele cenário e sendo por fim uma tentativa de humanização da fome. “Peraí, é nosso direito” destaca para o fato de que o Governo Federal assinou tratados internacionais que garantem o DHAA CONCLUSÃO: Os direitos humanos são imperativos para assegurar a todos a existência de uma vida digna. Os filmes documentais constituem meios de divulgação e denúncia a uma realidade que está posta, muitas vezes ignorada. Ressalta-se que nos meios acadêmicos faz-se vital que a formação de profissionais tenha delineação comprometida no debate de temas de dimensão social que tratam de seres socialmente vítimas da invisibilidade.

INTRODUCTIOB: Hunger and malnutrition are no longer prominent themes in contemporary media means. However, many arguments can still be raised about these issues that still persist in the present day of many Brazilians. OBJECTIVE: To analyze how Human Right to Adequate Nutrition from the content addressed in documentaries and national journalistic about hunger. METHODS: The study used audiovisual methods in order to perform an analytic-interpretative cut. It is qualitative in a perspective of documentary analysis, approaching a social reality denounced in television documentaries and journalistic news. RESULTS: The materials analyzed have in common the approach of delicate subjects that have been approached in a way that causes annoyance in the audience. “Garapa” is a film in a tone of social denunciation, portrayed in an unusual way for contemporary productions, showing the social disorder installed in that scenario and being at last an attempt to humanize hunger. “Peraí, it’s our right” highlights the fact that the Federal Government has signed international treaties guaranteeing the HRAN. CONCLUSION: Human rights are imperative to ensure the existence of a dignified life for all. Documentary films are means of dissemination and denunciation to a reality that is often ignored. It is emphasized that in the academic field it is vital that the training of professionals should have a delineation committed in the debate of social issues that deal with beings socially victims of invisibility.

Rice seed priming / Condicionamento fisiológico de sementes de arroz

The priming is a technique that involves the controlled hydration of seeds up to allows the start of germination preparatory processes without permit radicle protrusion. The aim of this study was to evaluate the effect of different procedures of priming on physiological potential of rice seeds. Seeds of rice cultivars Puita Inta Inov CL and CL, each represented by a lot were used. The treatments consisted of: control, hydropriming, priming with Stimulate®, boric acid solution and zinc sulfate solution, with four replications. Prior to priming, the seeds hydration curves were determining. After priming, the seeds evaluated for germination, seedling growth, efficiency of use of the endosperm, germination at lowest temperatures and seedling emergence. Priming procedures used in this study does not improve rice seed physiological potential, of cultivars Puita Inta CL and Inov Cl. Seed priming with boric acid has a negative effect on seed performance.

o condicionamento fisiológico é uma técnica que envolve a hidratação controlada das sementes que permite o início de processos de preparação para a germinação sem permitir que ocorra a protrusão da raiz. O objetivo deste estudo foi avaliar o efeito de diferentes procedimentos de condicionamento fisiológico no potencial fisiológico de sementes de arroz. Foram utilizadas sementes de arroz das cultivares Puita Inta CL e Inov CL, representadas por um lote cada. Os tratamentos consistiram de: controle, hidrocondicionamento, condicionamento com Stimulate®, com solução de ácido bórico e solução de sulfato de zinco, com quatro repetições. Previamente ao condicionamento, foram realizadas as curvas de hidratação. Após o condicionamento, as sementes foram avaliadas quanto à germinação, crescimento de plântulas, eficiência do uso do endosperma, germinação a baixas temperaturas e emergência das plântulas. Os procedimentos de condicionamento utilizados neste estudo não melhoraram o potencial fisiológico das sementes de arroz das cultivares de Puita Inta CL e Inov Cl. O condicionamento fisiológico de sementes de arroz com ácido bórico tem efeito negativo sobre o desempenho das sementes.

Pregnancy after uterine arterial embolization.

OBJECTIVE: To evaluate pregnancy outcomes, complications and neonatal outcomes in women who had previously undergone uterine arterial embolization. METHODS: A retrospective study of 187 patients treated with uterine arterial embolization for symptomatic uterine fibroids between 2005-2008 was performed. Uterine arterial embolization was performed using polyvinyl alcohol particles (500-900 mm in diameter). Pregnancies were identified using screening questionnaires and the study database. RESULTS: There were 15 spontaneous pregnancies. Of these, 12.5% were miscarriages (n = 2), and 87.5% were successful live births (n = 14). The gestation time for the pregnancies with successful live births ranged from 36 to 39.2 weeks. The mean time between embolization and conception was 23.8 months (range, 5-54). One of the pregnancies resulted in twins. The newborn weights (n = 14) ranged from 2.260 to 3.605 kg (mean, 3.072 kg). One (7.1%) was considered to have a low birth weight (2.260 kg). There were two cases of placenta accreta (12.5%, treated with hysterectomy in one case [6.3%]), one case of premature rupture of the membranes (PRM) (6.3%), and one case of preeclampsia (6.3%). All of the patients were delivered via Cesarean section. CONCLUSION: In this study, there was an increased risk of Cesarean delivery. There were no other major obstetric risks, suggesting that pregnancy after uterine arterial embolization is possible without significant morbidity or mortality.

Desempenho motor e senso de autoeficácia de escolares do ensino fundamental / Motor performance and self‑efficacy sense in students of elementary school / Desempeño motor y sentido de autoeficacia en alumnos de enseñanza primaria

Este estudo objetivou investigar o desempenho motor e o senso de autoeficácia de escolares do ensino fundamental, de uma escola municipal da cidade de São José/SC. Os participantes da pesquisa foram 338 crianças, com idade entre 7 e 10 anos. Para coleta dos dados, utilizaram a bateria para avaliação do movimento da criança (movement assessment battery for children – MABC) e o roteiro para avaliação do senso de autoeficácia (Rasae). Para análise dos dados, utilizou‑se estatística descritiva e inferencial. Quanto à classificação do desempenho motor, verificou‑se que o percentual de problemas motores definidos ultrapassou o índice apontado pela literatura. Com relação à autoeficácia, pode‑se dizer que, neste estudo, quanto melhor o desempenho motor, melhor foi o senso de autoeficácia.

This study aimed to investigate motor performance and sense of self‑efficacy of primary schools, a public school in the city of São José/SC. The participants were 338 children, aged between 7 and 10 years. To collect the data we used the Movement Assessment Battery for Children’s (MABC) and Assessment and Roadmap for the Sense of Self‑Efficacy (Rasae). For data analysis, we used descriptive and inferential statistics. The classification performance of the engine, it was found that the percentage of motor problems defined than the rate indicated by the literature. Regarding self‑efficacy can be said that in this study, the better the performance engine, the better the sense of self‑efficacy.

Este estudio tuvo como objetivo investigar el rendimiento motor y el sentido de la autoeficacia de los alumnos en una escuela pública de la ciudad de São José/SC. Los participantes fueron 338 niños, de edades compreendidas entre 7 y 10 años. Para recoger los datos se utilizó la bateria de evaluación del movimiento para niños (MABC) y una guia para evaluación del sentido de autoeficacia (Rasae). Para el análisis de los datos se utilizó estadística descriptiva e inferencial. En relación al desempeño motor fué verificado que el porciento de problemas motores definidos pasó el índice colocado por la literatura. Sobre la autoeficacia puede ser afirmado en el estudio que cuanto mejor era el desempeño motor mejor fué el sentido de la autoeficacia. La clasificación de rendimiento del motor se encontró en el porcentaje de problemas motores definidos en la indicada por la literatura. En cuanto a la autoeficacia se puede decir que entre mejor el rendimiento del motor, será mejor la sensación de autoeficacia.

Autoeficácia e desempenho escolar de alunos do ensino fundamental / Self-efficacy and school performance of elementary school students / Autoeficacia y desempeño escolar de alumnos de la enseñanza fundamental

O estudo objetivou investigar a associação do senso de autoeficácia com o desempenho escolar, as dificuldades de aprendizagem, o sexo e a idade de crianças das séries iniciais do Ensino Fundamental. Participaram 406 escolares (56,9% meninas), com idades entre sete e dez anos, matriculados em uma escola da região metropolitana de Florianópolis/SC. Os instrumentos utilizados foram o Teste de Desempenho Escolar (TDE) e o Roteiro de Avaliação do Senso de Autoeficácia (RASAE). Foi identificada associação significativa entre autoeficácia e desempenho escolar para todos os níveis de habilidades escolares, e aqueles com melhor desempenho apresentavam maior autoeficácia (p<0,001). Quanto ao sexo, as meninas mostraram-se mais autoeficazes do que os meninos (p<0,05). Crianças de diferentes grupos etários (7 a 8 e 9 a 10 anos) tiveram níveis semelhantes de autoeficácia. Sugere-se que ações com vista ao aumento da autoeficácia sejam direcionadas especialmente aos meninos e grupos com baixo desempenho, de forma a repercutirem na motivação e aprendizagem escolar.

In this study we aim at investigating the association of the feeling of self-efficacy with academic performance, learning difficulties, gender and age of children in the early grades of elementary school. The sample was composed of 406 children (56% girls), aged 7 to 10 years, enrolled in a school in the metropolitan area of Florianópolis/SC. The instruments used were the Academic Performance Test (TDE) and Sense of self-efficacy Assessment Guide (RASAE). Statistically significant association was found between self-efficacy and academic performance for all levels of school skills and those with the best performance had higher self-efficacy (p <0.001). Regarding the results between gender, girls were more self-efficacy than boys (p <0,05). Children of different age groups (7-8 and 9-10 years) had similar levels of self-efficacy. The results suggest that actions which target the increase of self-efficacy are directed especially to boys and groups with low academic performance. This produces impacts on the motivation and school learning.

El estudio tuvo por objetivo investigar la asociación del censo de autoeficacia con el desempeño escolar, las dificultades de aprendizaje, el sexo y la edad de niños de las series iniciales de la Enseñanza Fundamental. Participaron 406 escolares (el 56,9% de muchachas), con edades entre siete y diez años, inscriptos en una escuela de la región metropolitana de Florianópolis/SC. Los instrumentos utilizados fueron el test de Desempeño Escolar (TDE) y el Rutero de Evaluación del Censo de Autoeficacia (RASAE). Se identificó asociación significativa entre autoeficacia y desempeño escolar para todos los niveles de habilidades escolares, y aquellos con mejor desempeño presentaban mayor autoeficacia (p<0,001). Respeto al sexo, las muchachas se mostraron más auto eficaces de que los muchachos (p<0,05). Niños de distintos grupos eterios (7 a 8 y 9 a 10 años) tuvieron niveles semejantes de autoeficacia. Se sugiere que acciones con vista al aumento de la autoeficacia sean direccionadas especialmente a los muchachos y grupos con bajo desempeño, de forma a repercutir en la motivación y aprendizaje escolar.