Retinal function after intravitreal injection of autologous bone marrow-derived mesenchymal stromal cells in advanced glaucoma.

PURPOSE: To report electroretinographic (ERG) findings in advanced glaucoma treated with a single intravitreal injection of bone marrow-derived mesenchymal stem cells (MSCs). METHODS: Intravitreal injection of autologous MSCs (1 × 106 cells) was performed in 2 eyes from 2 patients with open-angle glaucoma in advanced stage of optic neuropathy (ClinicalTrials.gov, NCT02330978, 01.05.2015): cup/disk ratio worse than 0.9, visual field mean deviation index lower than - 15 dB, visual acuity of light perception, but controlled intraocular pressure. ERG tests were recorded at baseline and week 1, 4 and 48 after injection, using DTL electrodes following the ISCEV standard: After dark adaptation, ERG was elicited using white flashes of 0.01 cd.s/m2 and 3.0 cd.s/m2, followed by 10-min light adaptation (30 cd/m2) and stimuli of 3.0 cd.s/m2 and 30 Hz flicker. RESULTS: Patients did not show improvement on visual acuity or visual field after treatment. At baseline, ERG responses showed typical findings for advanced glaucoma, with a- and b-wave amplitude and latency within normative range, but reduced photopic negative responses. No noteworthy changes were observed on ERG responses for both cases up to 1 week after treatment, but at day 15, one patient showed retinal detachment with proliferative vitreoretinopathy and was removed from the trial. The other patient kept ERG responses stable throughout study period. CONCLUSION: Although no ERG response changes were observed after MSCs injection in one case, the complication observed on the second one, along with the lack of visual function improvement, warrants further studies involving modified MSCs to treat ocular disorders, including glaucoma. TRIAL REGISTRATION: ClinicalTrials.gov, NCT02330978- missed in pdf.

Estudo comparativo dos resultados das provas da prono-posiçäo em quarto escuro em indivíduos sentados e em decúbito horizontal ventral / Comparative analysis of the results of dark room prone provocative test of patients in sitting and ventral decubitus positions

Objetivo: Investigar os resultados das provas de prono-posiçäo em quarto escuro (PPQE) em indivíduos sentados e em decúbito horizontal ventral. Material e Métodos: Foram estudados 30 olhos de 15 pacientes portadores de glaucoma. As provas da PPQE foram realizadas nas duas posiçöes (sentado e deitado) com intervalo de 24 horas. Resultados: A média e o desvio padräo da diferença entre as pressöes intra-oculares finais e iniciais, do olho direito na posiçäo sentado foi de 4.13 ñ 1.77 mmHg e na posiçäo de decúbito horizontal ventral foi de 4.40 ñ 1.69 mmHg. No olho esquerdo na posiçäo sentado foi 4.13 ñ 1.69 mmHg e na posiçäo de decúbito horizontal ventral foi de 4.47 ñ 2.07 mmHg. Conclusäo: Näo foram encontradas diferenças estatísticamente significantes, entre as posiçöes sentado e em decúbito horizontal ventral, nos resultados da PPQE.