Graphene Derivatives in Biopolymer-Based Composites for Food Packaging Applications.

This review aims to showcase the current use of graphene derivatives, graphene-based nanomaterials in particular, in biopolymer-based composites for food packaging applications. A brief introduction regarding the valuable attributes of available and emergent bioplastic materials is made so that their contributions to the packaging field can be understood. Furthermore, their drawbacks are also disclosed to highlight the benefits that graphene derivatives can bring to bio-based formulations, from physicochemical to mechanical, barrier, and functional properties as antioxidant activity or electrical conductivity. The reported improvements in biopolymer-based composites carried out by graphene derivatives in the last three years are discussed, pointing to their potential for innovative food packaging applications such as electrically conductive food packaging.

Toxic impacts of rutile titanium dioxide in Mytilus galloprovincialis exposed to warming conditions.

Climate change is leading to a gradual increase in the ocean temperature, which can cause physiological and biochemical impairments in aquatic organisms. Along with the environmental changes, the presence of emerging pollutants such as titanium dioxide (TiO2) in marine coastal systems has also been a topic of concern, especially considering the interactive effects that both factors may present to inhabiting organisms. In the present study, it has been assessed the effects of the presence in water of particles of rutile, the most common polymorph of TiO2, in Mytilus galloprovincialis, under actual and predicted warming conditions. Organisms were exposed to different concentrations of rutile (0, 5, 50, 100 µg/L) at control (18 ± 1.0 °C) and increased (22 ± 1.0 °C) temperatures. Histopathological and biochemical changes were evaluated in mussels after 28 days of exposure. Histopathological examination revealed similar alterations on mussels' gills and digestive glands with increasing rutile concentrations at both temperatures. Biochemical markers showed that contaminated mussels have an unchanged metabolic capacity at 18 °C, which increased at 22 °C. Although antioxidant defences were activated in contaminated organisms at 22 °C, cellular damage was still observed. Overall, our findings showed that histopathological impacts occurred after rutile exposure regardless of the temperature, while biochemical alterations were only significantly noticeable when temperature was enhanced to 22 °C. Thus, this study demonstrated that temperature rise may significantly enhance the sensitivity of bivalves towards emerging pollutants.

Biochemical and histopathological impacts of rutile and anatase (TiO2 forms) in Mytilus galloprovincialis.

Titanium dioxide (TiO2) particles have been widely used in various industrial applications and consumer products. Due to their large production and use, they will eventually enter into aquatic environments. Once in the aquatic environment TiO2 particles may interact with the organisms and induce toxic effects. Since the most common crystallographic forms of TiO2 are rutile and anatase, the present study evaluated the effect of these two forms of TiO2 particles in Mytilus galloprovincialis. For this, mussels were exposed to different concentrations of rutile and anatase particles (0, 5, 50, 100 µg/L) for twenty-eight days. Ti concentrations, histopathological alterations and biochemical effects were evaluated. Similar Ti concentrations were found in mussels exposed to rutile and anatase, with the highest values in mussels exposed to the highest exposure concentration. Histopathological results demonstrated that both forms of TiO2 induced alterations on gills and digestive glands along the increasing exposure gradient. Biochemical markers showed that mussels exposed to rutile maintained their metabolic capacity (assessed by the activity of the Electron Transport System, ETS), while anatase increased the metabolism of mussels. Mussels exposed to rutile increased their detoxifying defences which, due to the low tested concentrations, were sufficient to avoid cellular damage. On the other hand, mussels exposed to anatase suffered cellular damages despite the increase of the antioxidant defences which may be related to the high ETS activity. Both rutile and anatase particles were toxic to M. galloprovincialis, being the highest oxidative stress exerted by the crystalline form anatase.