RESUMO
This study aimed to establish a rapid and practical method for monitoring and predicting volatile compounds during coffee roasting using near-infrared (NIR) spectroscopy coupled with chemometrics. Washed Arabica coffee beans from Ethiopia and Congo were roasted to industry-validated light, medium, and dark degrees. Concurrent analysis of the samples was performed using gas chromatography-mass spectrometry (GC-MS) and NIR spectroscopy, generating datasets for partial least squares (PLS) regression analysis. The results showed that NIR spectroscopy successfully differentiated the differently roasted samples, similar to the discrimination achieved by GC-MS. This finding highlights the potential of NIR spectroscopy as a rapid tool for monitoring and standardizing the degree of coffee roasting in the industry. A PLS regression model was developed using Ethiopian samples to explore the feasibility of NIR spectroscopy to indirectly measure the volatiles that are important in classifying the roast degree. For PLSR, the data underwent autoscaling as a preprocessing step, and the optimal number of latent variables (LVs) was determined through cross-validation, utilizing the root mean squared error (RMSE). The model was further validated using Congo samples and successfully predicted (with R2 values > 0.75 and low error) over 20 volatile compounds, including furans, ketones, phenols, and pyridines. Overall, this study demonstrates the potential of NIR spectroscopy as a practical and rapid method to complement current techniques for monitoring and predicting volatile compounds during the coffee roasting process.
Assuntos
Quimiometria , Espectroscopia de Luz Próxima ao Infravermelho , Etiópia , Furanos , Cromatografia Gasosa-Espectrometria de MassasRESUMO
BACKGROUND: This study investigated the geographical origin classification of green coffee beans from continental to country and regional levels. An innovative approach combined stable isotope and trace element analyses with non-linear machine learning data analysis to improve coffee origin classification and marker selection. Specialty green coffee beans sourced from three continents, eight countries, and 22 regions were analyzed by measuring five isotope ratios (δ13 C, δ15 N, δ18 O, δ2 H, and δ34 S) and 41 trace elements. Partial least squares discriminant analysis (PLS-DA) was applied to the integrated dataset for origin classification. RESULTS: Origins were predicted well at the country level and showed promise at the regional level, with discriminating marker selection at all levels. However, PLS-DA predicted origin poorly at the continental and Central American regional levels. Non-linear machine learning techniques improved predictions and enabled the identification of a higher number of origin markers, and those that were identified were more relevant. The best predictive accuracy was found using ensemble decision trees, random forest and extreme gradient boost, with accuracies of up to 0.94 and 0.89 for continental and Central American regional models, respectively. CONCLUSION: The potential for advanced machine learning models to improve origin classification and the identification of relevant origin markers was demonstrated. The decision-tree-based models were superior with their embedded variable identification features and visual interpretation. © 2023 The Authors. Journal of The Science of Food and Agriculture published by John Wiley & Sons Ltd on behalf of Society of Chemical Industry.
Assuntos
Aprendizado de Máquina , Isótopos/química , Oligoelementos/química , Dinâmica não Linear , Café/químicaRESUMO
Stable isotope ratios and trace elements are well-established tools that act as signatures of the product's environmental conditions and agricultural processes; but they involve time, money, and environmentally destructive chemicals. In this study, we tested for the first time the potential of near-infrared reflectance spectroscopy (NIR) to estimate/predict isotope and elemental compositions for the origin verification of coffee. Green coffee samples from two continents, 4 countries, and 10 regions were analysed for five isotope ratios (δ13C, δ15N, δ18O, δ2H, and δ34S) and 41 trace elements. NIR (1100-2400 nm) calibrations were developed using pre-processing with extended multiplicative scatter correction (EMSC) and mean centering and partial-least squares regression (PLS-R). Five elements (Mn, Mo, Rb, B, La) and three isotope ratios (δ13C, δ18O, δ2H) were moderately to well predicted by NIR (R2: 0.69 to 0.93). NIR indirectly measured these parameters by association with organic compounds in coffee. These parameters were related to altitude, temperature and rainfall differences across countries and regions and were previously found to be origin discriminators for coffee.
Assuntos
Café , Oligoelementos , Café/química , Oligoelementos/análise , Isótopos de Oxigênio/análise , Espectroscopia de Luz Próxima ao Infravermelho , Análise dos Mínimos QuadradosRESUMO
The potential of using rapid and non-destructive near-infrared - hyperspectral imaging (HSI-NIR) for the prediction of an integrated stable isotope and multi-element dataset was explored for the first time with the help of support vector regression. Speciality green coffee beans sourced from three continents, eight countries, and 22 regions were analysed using a push-broom HSI-NIR (700-1700 nm), together with five isotope ratios (δ13C, δ15N, δ18O, δ2H, and δ34S) and 41 trace elements. Support vector regression with the radial basis function kernel was conducted using X as the HSI-NIR data and Y as the geochemistry markers. Model performance was evaluated using root mean squared error, coefficient of determination, and mean absolute error. Three isotope ratios (δ18O, δ2H, and δ34S) and eight elements (Zn, Mn, Ni, Mo, Cs, Co, Cd, and La) had an R2predicted 0.70 - 0.99 across all origin scales (continent, country, region). All five isotope ratios were well predicted at the country and regional levels. The wavelength regions contributing the most towards each prediction model were highlighted, including a discussion of the correlations across all geochemical parameters. This study demonstrates the feasibility of using HSI-NIR as a rapid and non-destructive method to estimate traditional geochemistry parameters, some of which are origin-discriminating variables related to altitude, temperature, and rainfall differences across origins.
Assuntos
Oligoelementos , Oligoelementos/análise , Imageamento Hiperespectral , Isótopos , Espectroscopia de Luz Próxima ao InfravermelhoRESUMO
This study focuses on the details of consumer response to lab grown 'cultured meat (CM)', compared to meat derived from insects, plants and animals. A sample of 254 New Zealanders were interviewed. A word association exercise revealed that consumer reaction to CM was dominated by affective, rather than cognitive factors. The linkages between a general food neophobia scale, a specific CM evaluation scale and purchase intent were studied. The general neophobia scale performed poorly as a predictor, while the 19-point CM evaluation scale performed well. Reducing this scale to its seven affective components, and then to just the two key affective components did not significantly reduce the scale's predictive performance. Overall, the results of this research reveal very significant differences in preference for meat products based upon their origins. Insect protein was strongly disfavoured over all alternatives, while cultured meat was significantly disfavoured compared to more established alternatives. The implications of this for the commercialisation of CM are discussed.