RESUMO
Cercospora sesami is a plant pathogen that causes leaf spot disease in sesame plants worldwide. In this study, genome sequence assembly of C. sesami isolate Cers 52-10 (MCC 9069) was generated using native paired-end and mate-pair DNA sequencing based on the Illumina HiSeq 2500 platform. The genome assembly of C. sesami is 34.3 Mb in size with an N50 of 26,222 bp and an average GC content of 53.02%. A total number of 10,872 genes were predicted in this study, out of which 9,712 genes were functionally annotated. Genes assigned to carbohydrate-active enzyme classes were also identified during the study. A total of 80 putative effector candidates were predicted and functionally annotated. The C. sesami genome sequence is available at DDBJ/ENA/GenBank, and other associated information is submitted to Mendeley's data. Supplementary Information: The online version contains supplementary material available at 10.1007/s13205-023-03468-4.
RESUMO
AIM: This study set out to compare the damage done to dentin by three distinct titanium file brands - the Hyflex EDM, the ProTaper Next, and the Waveone Gold Nickel - in order to draw conclusions about which one is the most effective. MATERIALS AND METHODS: Forty-first premolars in the mandible with straight canals and single roots were instrumented using Hyflex EDM, Waveone Gold, and Protaper Next. Dentinal flaws after endodontic treatment were studied by sectioning specimens using a hard tissue microtome and analyzing them under a stereomicroscope. RESULTS: There was no discernible variation between the groups in the coronal third (p=0.312) or apical third (p=0.076). Hyflex EDM and Protaper Next differed significantly in the middle portion of the tape (p=0.016). The Hyflex EDM sample had the fewest cracks. There was no statistically significant difference between Hyflex EDM and Waveone Gold; however, Hyflex EDM had fewer fractures in the middle third of the sample than Waveone Gold did. CONCLUSION: Electric discharge machining (EDM) files made from Hyflex proved to be far superior to their Protaper Next and Waveone Gold counterparts as they induced the fewest cracks in the middle third of the root dentin.
RESUMO
Draft genomes of the fungal species Ambrosiella cleistominuta, Cercospora brassicicola, C. citrullina, Physcia stellaris, and Teratosphaeria pseudoeucalypti are presented. Physcia stellaris is an important lichen forming fungus and Ambrosiella cleistominuta is an ambrosia beetle symbiont. Cercospora brassicicola and C. citrullina are agriculturally relevant plant pathogens that cause leaf-spots in brassicaceous vegetables and cucurbits respectively. Teratosphaeria pseudoeucalypti causes severe leaf blight and defoliation of Eucalyptus trees. These genomes provide a valuable resource for understanding the molecular processes in these economically important fungi.
RESUMO
BACKGROUND: Hospital-based registries are important tools for policy formulations and region-specific data creation, particularly in case of cervical cancer, which is preventable by an effective screening program. OBJECTIVE: To study the epidemiological characteristics and trends in cervical neoplasia in an urban population over a decade. MATERIALS AND METHODS: Data were collected from the histopathological records of patients from January 2000 to December 2009. RESULTS: A total of 1315 gynecological malignancies were reported with cancer cervix being the most common (70.4%) with a progressive increase in the number from 56 in 2000 to 157 in 2009. Squamous cell cancer was the predominant variant with majority in advanced stage. The mean age was 50.1 years with doubling of cases in the ≤35-year category. Of 742 colposcopic biopsies performed, cervical intraepithelial neoplasia (CIN) 1 was diagnosed in 34.9%, CIN 2 in 11.8%, CIN 3 in 7.8%, and microinvasive cancer in 0.8% cases. CONCLUSION: Though screening programs are in place, their effectiveness can be judged only by such reporting systems that aid in modifying the current cancer control strategies.