Quantification of resting myocardial blood flow velocity in normal humans using real-time contrast echocardiography. A feasibility study.

BACKGROUND: Real-time myocardial contrast echocardiography (MCE) is a novel method for assessing myocardial perfusion. The aim of this study was to evaluate the feasibility of a very low-power real-time MCE for quantification of regional resting myocardial blood flow (MBF) velocity in normal human myocardium. METHODS: Twenty study subjects with normal left ventricular (LV) wall motion and normal coronary arteries, underwent low-power real-time MCE based on color-coded pulse inversion Doppler. Standard apical LV views were acquired during constant IV. infusion of SonoVue. Following transient microbubble destruction, the contrast replenishment rate (beta), reflecting MBF velocity, was derived by plotting signal intensity vs. time and fitting data to the exponential function; y (t) =A (1-e(-beta(t-t0))) + C. RESULTS: Quantification was feasible in 82%, 49% and 63% of four-chamber, two-chamber and apical long-axis view segments, respectively. The LAD (left anterior descending artery) and RCA (right coronary artery) territories could potentially be evaluated in most, but contrast detection in the LCx (left circumflex artery) bed was poor. Depending on localisation and which frames to be analysed, mean values of beta were 0.21-0.69 s(-1), with higher values in medial than lateral, and in basal compared to apical regions of scan plane (p = 0.03 and p < 0.01). Higher beta-values were obtained from end-diastole than end-systole (p < 0.001), values from all-frames analysis lying between. CONCLUSION: Low-power real-time MCE did have the potential to give contrast enhancement for quantification of resting regional MBF velocity. However, the technique is difficult and subjected to several limitations. Significant variability in beta suggests that this parameter is best suited for with-in patient changes, comparing values of stress studies to baseline.

Quantitative adenosine real-time myocardial contrast echocardiography for detection of angiographically significant coronary artery disease.

BACKGROUND: Real-time (RT) myocardial contrast echocardiography (MCE) is a novel method for assessment of regional myocardial perfusion. We sought to evaluate the feasibility and diagnostic accuracy of quantitative adenosine RT MCE in predicting significant coronary stenoses, with reference to quantitative coronary angiography. METHODS: Low-power RT MCE was performed in 43 patients scheduled for quantitative coronary angiography. Peak signal intensity (A), rate of signal intensity increase (beta), A x beta (myocardial blood flow), and their hyperemic reserves were estimated and compared with angiographic data. RESULTS: The feasibility of quantitative stress RT MCE covering all coronary territories was 77% of patients with adequate baseline image quality. At rest we found no significant difference for any of the perfusion parameters between the normal and stenosed coronary territories. During hyperemia, beta and A x beta, but not A, increased significantly in normal coronary territories. In the regions subtended by significantly stenosed arteries, there were no significant increases in beta and A x beta. Receiver operating characteristic curves indicated that beta- and A x beta-reserves, but not A-reserve, could be sensitive parameters for detecting flow-limiting coronary stenosis in selected patients, particularly if significant left anterior descending coronary artery disease was involved. CONCLUSION: Quantitative assessment of myocardial blood flow and its velocity reserve by RT MCE has the potential to detect significant coronary artery disease, but because of imaging and technical problems it is not yet robust enough for clinical use in unselected patients.

Effects of ultrasound contrast during tissue velocity imaging on regional left ventricular velocity, strain, and strain rate measurements.

BACKGROUND: Strain (epsilon) rate (SR) imaging and left ventricular (LV) opacification with intravenous (IV) contrast both potentially decrease operator dependency in interpretation of stress echocardiography. The aim of this study was to evaluate whether contrast present during tissue velocity imaging (TVI) significantly affected measurements of velocity, epsilon, and SR. Secondly, we sought to evaluate whether increased scan line density improved feasibility of simultaneous TVI and contrast echocardiography. METHODS: The 4-chamber LV view in 15 healthy volunteers and 25 patients was acquired at rest before and after IV injections of contrast using: (1) conventional TVI; (2) LV opacification with standard TVI added; and (3) modified LV opacification with doubled TVI line density. Velocity, SR, and epsilon curves, along with peak systolic velocity, peak systolic SR, and end-systolic epsilon, were assessed from midwall segments. RESULTS: IV contrast significantly reduced feasibility of TVI with standard settings, giving noisy data for SR and epsilon, particularly in the septum. Absolute values of peak systolic SR and end-systolic epsilon from adequately shaped curves were significantly higher with contrast compared with baseline. However, increased TVI line density significantly improved feasibility of velocity traces with contrast and decreased the level of noise in SR and epsilon. Furthermore, higher line density improved agreement between peak systolic velocity, peak systolic SR, and end-systolic epsilon measured with contrast, and corresponding precontrast values from the conventional TVI setting. CONCLUSIONS: SR imaging was not feasible performed with IV contrast during conventional TVI settings, and we do not recommend the clinical use of this combination. Increased TVI line density made velocity curves with contrast feasible and resulted in less noisy SR and epsilon curves, but variability in SR and epsilon measurements with contrast is still too high for clinical use.

Real-time simultaneous triplane contrast echocardiography gives rapid, accurate, and reproducible assessment of left ventricular volumes and ejection fraction: a comparison with magnetic resonance imaging.

OBJECTIVE: We sought to compare the feasibility, accuracy, and reproducibility of simultaneous triplane echocardiography for measurements of left ventricular (LV) volumes and ejection fraction (EF) with reference to magnetic resonance imaging (MRI). METHODS: Digital echocardiography recordings of apical LV views with and without intravenous contrast were collected from 53 consecutive patients with conventional 2-dimensional (2D) imaging and with simultaneous triplane imaging. MRI of multiple LV short-axis sections was performed with a 1.5-T scanner. Endocardial borders were manually traced, and LV volumes and EF from 2D biplane echocardiography and MRI were calculated by method of disks. On triplane data, a triangular mesh was constructed by 3-dimensional interpolation and volumes calculated by the divergence theorem. RESULTS: Triplane image acquisition was less time-consuming than 2D biplane. Precontrast feasibility was 72% for triplane and 82% for 2D biplane images, increasing to 98% and 100% with contrast, respectively. Bland-Altman analysis demonstrated LV volume underestimation by echocardiography versus MRI, which was significantly reduced by contrast and triplane imaging. The 95% limits of agreement for EF between echocardiography and MRI narrowed using triplane compared with 2D biplane (precontrast -12.5 to 6.7% vs -17.2 to 9.9%, and with contrast -7.1 to 5.8% vs -9.4 to 6.4%, respectively). At intraobserver and interobserver analysis of 20 patients, limits of agreement for EF narrowed with contrast triplane compared with 2D biplane. CONCLUSION: Simultaneous LV triplane imaging is feasible with simple and rapid image acquisition and volume analysis, and with contrast enhancement it gives accurate and reproducible LV EF measurements compared with MRI.