RESUMO
Resistance to minocycline has emerged in multidrug-resistant Acinetobacter baumannii isolates from Buenos Aires hospitals. Few reports about the description and dispersion of tet genes in this species have been published. We observed the presence of tet(B) in all minocycline-resistant isolates. This gene was found to be associated with the ISCR2 mobile element, which may, in part, explain its dispersion.
Assuntos
Infecções por Acinetobacter/microbiologia , Acinetobacter baumannii/genética , Antibacterianos/uso terapêutico , Minociclina/uso terapêutico , Plasmídeos , Resistência a Tetraciclina/genética , Infecções por Acinetobacter/tratamento farmacológico , Infecções por Acinetobacter/epidemiologia , Acinetobacter baumannii/isolamento & purificação , Antibacterianos/farmacologia , Argentina/epidemiologia , Sequência de Bases , Humanos , Testes de Sensibilidade Microbiana , Minociclina/farmacologia , Dados de Sequência Molecular , Retroelementos , Análise de Sequência de DNAAssuntos
Antibacterianos/farmacologia , Proteínas de Bactérias , Carbapenêmicos/farmacologia , Surtos de Doenças , Infecções por Pseudomonas/epidemiologia , Pseudomonas aeruginosa/efeitos dos fármacos , Resistência beta-Lactâmica , beta-Lactamases , Argentina/epidemiologia , Proteínas de Bactérias/biossíntese , Ceftazidima/farmacologia , Humanos , Testes de Sensibilidade Microbiana/métodos , Infecções por Pseudomonas/microbiologia , Pseudomonas aeruginosa/enzimologia , Pseudomonas aeruginosa/isolamento & purificação , beta-Lactamases/biossínteseRESUMO
The aim of this study was to evaluate in vitro the marginal microleakage of two glass ionomer materials used as pit and fissure sealants. Thirty healthy premolars extracted for orthodontic treatment were randomly assigned to two groups (n=15) and respectively sealed with two glass ionomers (Group I, Fuji VII and Group II, Fuji IX). All teeth were preserved in artificial saliva (NAF) for 10 days, thermocycled (250 cycles; 5 °C, 37 °C and 60 °C), isolated, and immersed in 2% alcohol gentian violet blue solution for 24 h. After washing, teeth were included in acrylic resin and sectioned longitudinally in a bucco-lingual direction with a Struers-Minitom cutting device. Samples were analyzed for leakage using an optical microscope (Olympus BX- 60M). TheWilliams and Winter semi-quantitative ranked scale was used to score dye penetration. In Group I the grades were distributed as follows: Grade 1, 1 sample and Grade 3, 14 samples (Mean 2.87 Median 3, SD 0.52). In Group II: Grade 0: 4 samples, Grade 1, 3 samples, Grade 2, 2 samples and Grade 3, 6 samples (Mean 1.67, Median 2, SD 1.29). Fisher's exact test showed statistically significant differences between materials (p=0.006). From these results, we conclude that Fuji IX had better marginal sealing than Fuji VII when used as a pit and fissure sealant.
Assuntos
Cimentos de Ionômeros de Vidro , Infiltração Dentária , Humanos , Selantes de Fossas e FissurasAssuntos
Queimaduras/microbiologia , Infecções por Pseudomonas/diagnóstico , Pseudomonas mendocina/isolamento & purificação , beta-Lactamases/genética , Adulto , Queimaduras/cirurgia , Colistina/uso terapêutico , Evolução Fatal , Humanos , Masculino , Pessoa de Meia-Idade , Insuficiência de Múltiplos Órgãos/etiologia , Infecções por Pseudomonas/tratamento farmacológico , Pseudomonas mendocina/genética , Transplante de Pele , Resultado do TratamentoRESUMO
El objetivo del trabajo fue comparar la eficacia como sellador de dos ionómeros vítreos. Se emplearon 30 premolares sanos extraídos por razones ortodóncicas. Se dividieron en dos grupos iguales (n=15) .Grupo I: Fuji VII y Grupo II: Fuji IX. Los dientes, fueron conservados en saliva artificial (NAF) durante 10 días. Luego, fueron termociclados (250 ciclos) a 5ºC, 37ºC y60ºC. Posteriormente se sumergieron en violeta de genciana durante 24 hs. Una vez lavadas las muestras se seccionaron y evaluaron con el criterio de Williams y Winter, utilizando un microscopio óptico (Olympus BX-60M) para valorar la penetración del colorante. Los grados obtenidos para cada grupo fueron: en Grupo I (Fuji VII), Grado 1, 1 muestra y Grado3, 14 muestras (Media 2,87, Mediana 3 y SD 0.52). En el Grupo II (Fuji IX) los grados se distribuyeron así: Grado 0, 4 muestras, Grado 1, 3 muestras, Grado 2, 2 muestras y Grado 3, 6 muestras.(Media 1.67, Mediana 2 y SD 1.29). La Prueba exacta de Fisher (p=0,006) demostró diferencias estadísticamente significativa entre materiales. Se concluyó que el mejor sellado marginal fue obtenido utilizando el material Fuji IX comparado con Fuji VII cuando es utilizado como sellador.
The aim of this study was to evaluate in vitro the marginalmicroleakage of two glass ionomer materials used as pit andfissure sealants. Thirty healthy premolars extracted fororthodontic treatment were randomly assigned to two groups(n=15) and respectively sealed with two glass ionomers (Group I,Fuji VII and Group II, Fuji IX). All teeth were preserved inartificial saliva (NAF) for 10 days, thermocycled (250 cycles; 5ºC,37ºC and 60ºC), isolated, and immersed in 2% alcohol gentianviolet blue solution for 24 h. After washing, teeth were included inacrylic resin and sectioned longitudinally in a bucco-lingualdirection with a Struers-Minitom cutting device. Samples wereanalyzed for leakage using an optical microscope (Olympus BX-60M). TheWilliams and Winter semi-quantitative ranked scale wasused to score dye penetration. In Group I the grades weredistributed as follows: Grade 1, 1 sample and Grade 3, 14 samples(Mean 2.87 Median 3, SD 0.52). In Group II: Grade 0: 4 samples,Grade 1, 3 samples, Grade 2, 2 samples and Grade 3, 6 samples(Mean 1.67, Median 2, SD 1.29). Fishers exact test showedstatistically significant differences between materials (p=0.006).From these results, we conclude that Fuji IX had better marginalsealing than Fuji VII when used as a pit and fissure sealant.