RESUMO
The aim of this study was to verify the applicability of high-concentration collagen-based bioink with MSC (ADSC) and decellularized ECM granules for the formation of cartilage tissue de novo after subcutaneous implantation of the scaffolds in rats. The printability of the bioink (4% collagen, 2.5% decellularized ECM granules, derived via 280 µm sieve) was shown. Three collagen-based compositions were studied: (1) with ECM; (2) with MSC; (3) with ECM and MSC. It has been established that decellularized ECM granules are able to stimulate chondrogenesis both in cell-free and MSC-laden scaffolds. Undesirable effects have been identified: bone formation as well as cartilage formation outside of the scaffold area. The key perspectives and limitations of ECM granules (powder) application have been discussed.
Assuntos
Bioimpressão , Condrogênese , Animais , Cartilagem , Colágeno , Matriz Extracelular Descelularizada , Matriz Extracelular , Impressão Tridimensional , Ratos , Engenharia Tecidual , Alicerces TeciduaisRESUMO
We present a new method for investigation of sensitivity of the integrated optical electrodeless electric field sensor. This method is based on a compact microwave asymmetrical double-ridged waveguide with regular inhomogeneities. Small dimensions of the waveguide along with medium power of the microwave generator produce a strong electric field. The efficiency of the presented experimental unit was verified in the course of testing of the real integrated optical electrodeless electric field sensor (E-sensor).