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1.
J Nanosci Nanotechnol ; 19(8): 4724-4726, 2019 Aug 01.
Artigo em Inglês | MEDLINE | ID: mdl-30913777

RESUMO

Organic photodetector (OPD) performance is affected significantly by leakage current. In this study, to decrease OPD leakage current, we introduced hafnium oxide as an electron blocking material, using a solution fabrication process. We fabricated an OPD consisting of ITO/HfO2/PCHT:PC60BM/Yb/Al, and measured its J-V characteristics, external quantum efficiency, and transient photocurrents. We found that the thickness of the hafnium oxide layer affected the detectivity of the prepared OPD. In particular, a device having an ultrathin hafnium oxide film (5.5 nm thick) exhibited a high on-off current ratio of up to 2.26 × 105 at -1 V, which is two times higher than that of a device having a PEDOT:PSS electron blocking layer.

2.
Gut ; 65(2): 305-12, 2016 Feb.
Artigo em Inglês | MEDLINE | ID: mdl-26045140

RESUMO

OBJECTIVES: Serous cystic neoplasm (SCN) is a cystic neoplasm of the pancreas whose natural history is poorly known. The purpose of the study was to attempt to describe the natural history of SCN, including the specific mortality. DESIGN: Retrospective multinational study including SCN diagnosed between 1990 and 2014. RESULTS: 2622 patients were included. Seventy-four per cent were women, and median age at diagnosis was 58 years (16-99). Patients presented with non-specific abdominal pain (27%), pancreaticobiliary symptoms (9%), diabetes mellitus (5%), other symptoms (4%) and/or were asymptomatic (61%). Fifty-two per cent of patients were operated on during the first year after diagnosis (median size: 40 mm (2-200)), 9% had resection beyond 1 year of follow-up (3 years (1-20), size at diagnosis: 25 mm (4-140)) and 39% had no surgery (3.6 years (1-23), 25.5 mm (1-200)). Surgical indications were (not exclusive) uncertain diagnosis (60%), symptoms (23%), size increase (12%), large size (6%) and adjacent organ compression (5%). In patients followed beyond 1 year (n=1271), size increased in 37% (growth rate: 4 mm/year), was stable in 57% and decreased in 6%. Three serous cystadenocarcinomas were recorded. Postoperative mortality was 0.6% (n=10), and SCN's related mortality was 0.1% (n=1). CONCLUSIONS: After a 3-year follow-up, clinical relevant symptoms occurred in a very small proportion of patients and size slowly increased in less than half. Surgical treatment should be proposed only for diagnosis remaining uncertain after complete workup, significant and related symptoms or exceptionally when exists concern with malignancy. This study supports an initial conservative management in the majority of patients with SCN. TRIAL REGISTRATION NUMBER: IRB 00006477.


Assuntos
Cistadenoma Seroso , Neoplasias Pancreáticas , Adolescente , Adulto , Idoso , Idoso de 80 Anos ou mais , Cistadenoma Seroso/diagnóstico , Cistadenoma Seroso/mortalidade , Cistadenoma Seroso/patologia , Cistadenoma Seroso/terapia , Europa (Continente) , Feminino , Humanos , Internacionalidade , Masculino , Pessoa de Meia-Idade , Neoplasias Pancreáticas/diagnóstico , Neoplasias Pancreáticas/mortalidade , Neoplasias Pancreáticas/patologia , Neoplasias Pancreáticas/terapia , Estudos Retrospectivos , Sociedades Médicas , Adulto Jovem
3.
Poult Sci ; 95(3): 645-50, 2016 Mar.
Artigo em Inglês | MEDLINE | ID: mdl-26574035

RESUMO

The effects of postmortem (PM) temperature on prerigor Pekin duck breast muscle quality were assessed. Breast meat was obtained from 90 ducks within 15 min PM and then divided into 3 storage temperature groups at 0, 15, and 30°C for 24 h PM. Results revealed that the meat stored at 0°C had a higher pH value than that stored at 30°C. The R-value tended to increase between 15 min, 2 h, and 24 h PM, whereas the water-holding capacity decreased significantly (P < 0.05) with increasing storage time. The drip loss of treatment in meat stored at 30°C was higher than in those stored at 0 and 15°C. As storage periods increased, cooking loss of meat stored at each temperature increased significantly (P < 0.05) at 2 h, but storage temperature does not affect cooking loss of duck breast muscle. The shear force of breast meat at 24 h PM had the lowest value, but meat stored at 30°C increased at 2 h and decreased at 24 h PM. Meat stored at 15°C showed a longer sarcomere length than meat stored at 0 and 30°C. The rate of muscle shortening was high during the 2 h PM for meat at the 3 temperatures. It is concluded that the different temperatures in the range of 0 to 30°C affected the muscle shortening or meat quality of the duck breast meat.


Assuntos
Patos/fisiologia , Armazenamento de Alimentos , Carne/análise , Músculos Peitorais/fisiologia , Temperatura , Animais , Culinária , Músculos Peitorais/química , Fatores de Tempo
4.
Spinal Cord ; 52 Suppl 1: S5-6, 2014 Jun.
Artigo em Inglês | MEDLINE | ID: mdl-24902645

RESUMO

STUDY DESIGN: Case report. OBJECTIVE: To present a case of spinal cord injury (SCI)-associated neuropathic pain treated with botulinum toxin A injection. SETTING: Outpatient SCI clinic. CASE REPORT: Description of a case in the context of relevant literature on the subject. RESULTS: A 51-year-old man with C3 AIS B tetraplegia (American Spinal Injury Association Impairment Scale B) visited our outpatient clinic due to severe ongoing neuropathic pain and associated allodynia and dysesthesia of the lower limbs. He had previously tried combinations of pregabalin, gabapentin and oxycontin but all failed to alleviate his neuropathic pain during the 2 years post trauma. With the patient's consent, he was treated with subcutaneous injections of clostridium type A botulinum toxin. Ten units of type A botulinum toxin subcutaneously injected into 10 most painful sites of each sole. The patient was reassessed at 4 and 8 weeks after his injection with botulinum toxin with significant improvement of his neuropathic pain. CONCLUSION: Subcutaneous injection of type A botulinum toxin was effective without side effects, on one case of refractory neuropathic pain due to SCI. This is the first article to report the use of type A botulinum toxin in neuropathic pain related to traumatic SCI.


Assuntos
Toxinas Botulínicas Tipo A/administração & dosagem , Neuralgia/tratamento farmacológico , Neuralgia/etiologia , Fármacos Neuromusculares/uso terapêutico , Traumatismos da Medula Espinal/complicações , Humanos , Injeções Subcutâneas , Masculino , Pessoa de Meia-Idade , Medição da Dor
5.
Poult Sci ; 93(3): 680-6, 2014 Mar.
Artigo em Inglês | MEDLINE | ID: mdl-24604862

RESUMO

The objective of this study was to evaluate the effects of soy sauce on the physicochemical and textural properties of tumbled chicken breasts. Chicken breasts marinated with distilled water (Con), 4% NaCl solution, 4% NaCl and lactic acid solution (pH 4.9), and soy sauce solution (4% salt concentration and pH 4.9) were vacuum tumbled at 3°C for 60 min. The chicken breast marinated with soy sauce solution showed lower lightness and higher redness and yellowness due to the color of the soy sauce. The acidic marinades led to a decrease in pH value of tumbled chicken breast. The acidic marinades increased collagen solubility of sample compared with 4% NaCl solution, resulting in decreased shear force. Water-holding capacity, marination and cooking yields, and solubility of myofibrillar proteins were mainly affected by the presence of salt in the marinade, rather than by pH alternation. Our results suggested that soy sauce marination can improve the tenderness of tumbled chicken breast.


Assuntos
Galinhas/fisiologia , Manipulação de Alimentos , Carne/normas , Alimentos de Soja/análise , Animais , Culinária , Concentração de Íons de Hidrogênio , Músculos Peitorais/fisiologia , Cloreto de Sódio
6.
Poult Sci ; 102(3): 102444, 2023 Mar.
Artigo em Inglês | MEDLINE | ID: mdl-36603520

RESUMO

The objective of this study was to evaluate the general quality attributes and shelf stability of reduced-salt and sous-vide cooked chicken breast hams during 4 weeks of refrigerated storage (4°C). Four treatment groups of chicken breast ham were prepared using a 2 (salt level, 1.5% NaCl (regular) and 0.75% (reduced)) × 2 (cooking method, conventional and sous-vide) factorial arrangement. Based on each chicken breast weight, 20% NaCl solution was injected. Conventional cooking was done at 80°C until the core temperature reached 71°C, whereas sous-vide cooking was conducted at 60°C for 2 h. Sous-vide cooking could decrease cooking loss and shear force of reduced-salt chicken breast ham (P < 0.05). As a result, sensory scores for juiciness and tenderness of reduced-salt and sous-vide cooked chicken breast ham were similar to those of regular-salt and conventionally cooked chicken breast hams (P > 0.05). No adverse impacts on lipid oxidation and microbial safety were found in reduced-salt and sous-vide cooked chicken breast ham during 4 wk of refrigerated storage. Therefore, this study suggests that sous-vide cooking could be a practical thermal process for improving the water-holding capacity and texture of chicken breast ham without adverse impacts on shelf stability. Further studies on the combined application of sous-vide cooking with salt replacers would be warranted to improve the sensorial acceptance of saltiness of sous-vide cooked low-salt meat products.


Assuntos
Produtos da Carne , Carne , Animais , Carne/análise , Galinhas , Cloreto de Sódio , Culinária/métodos , Produtos da Carne/análise
7.
Poult Sci ; 91(10): 2662-7, 2012 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-22991555

RESUMO

The objectives of this study were to evaluate effects of rigor state, thawing temperature, and processing on the physicochemical properties of intact frozen duck breast muscle and homogenates prepared with the frozen-thawed muscles. Prerigor breast muscle was frozen at -25°C and thawed at 4 and 15°C, and the physicochemical properties of duck breast frozen prerigor compared with those in frozen postrigor and 4°C chilled postrigor muscles. The homogenates prepared with duck breast frozen prerigor showed similar physicochemical properties as the frozen-thawed postrigor muscle homogenate. The thawing temperature did not affect the physicochemical properties of homogenates prepared with duck breast muscle frozen prerigor. Also, no significant differences (P > 0.05) in the ultimate pH value and color parameters were observed among the frozen-thawed treatments despite the differences in rigor state and thawing temperature. However, a higher thawing temperature of the intact frozen prerigor muscle resulted in an increase in thawing loss and shear force due to excessive muscle shortening. In this study, the intact duck breast that has undergone thaw-rigor showed main problems associated with thaw-rigor, such as higher thawing loss and shear force, regardless of thawing temperature. However, the processing (grinding and salting) decreased the difference in physicochemical properties due to rigor state before freezing and thawing temperature. Therefore, the frozen-thawed duck breast muscle used to manufacture ground-type meat product may be frozen before rigor onset.


Assuntos
Carne/normas , Músculo Esquelético/fisiologia , Animais , Patos , Manipulação de Alimentos , Temperatura
8.
Zhonghua Shao Shang Za Zhi ; 36(9): 873-875, 2020 Sep 20.
Artigo em Chinês | MEDLINE | ID: mdl-32972075

RESUMO

On January 10, 2018, a 35-year-old male patient with a 3 day stingray stinger injury in his left thigh was admitted to Xuzhou Central Hospital. At the time of admission, the patient's left thigh was red, swollen, and painful. On the day of admission, the patient underwent emergency operation in the outpatient operating room for local debridement to remove the infected and necrotic tissue. After the routine dressing change and the wound got better, surgical debridement and negative-pressure wound therapy were performed, and finally local flap was used to repair the wound. On the 14th day after the flap repair operation, the suture was removed and the patient was discharged. After half a year follow-up, the appearance of the operation area recovered well. This case suggests that the timely and correct pre-hospital treatment, thorough and timely debridement, and systemic antibiotic application are important means to reduce further injury of toxin. The local flap can achieve satisfactory result on wound healing after the wound bed is prepared by negative-pressure wound therapy.


Assuntos
Mordeduras e Picadas/terapia , Tratamento de Ferimentos com Pressão Negativa , Procedimentos de Cirurgia Plástica , Rajidae , Lesões dos Tecidos Moles , Adulto , Animais , Desbridamento , Humanos , Masculino , Transplante de Pele , Lesões dos Tecidos Moles/cirurgia , Resultado do Tratamento
9.
Poult Sci ; 99(1): 597-603, 2020 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-32416846

RESUMO

The objective of this study was to determine the impact of pre-rigor salting using KCl on the technological properties of ground chicken breast. Chicken breast muscle (M. pectoralis major and minor) was hot-debonded and salted with 2% NaCl (w/w), 1% NaCl+1% KCl mixture, or 2% KCl, respectively, within 30 min after slaughter. Post-rigor salting treatment was prepared with 2% NaCl at 24 h postmortem. All pre-rigor salting treatments showed higher ultimate pH, protein solubility, and final yield than post-rigor salting treatment (P < 0.05). However, the positive effects of pre-rigor salting on chicken breast differed by salt type. Pre-rigor salting with KCl resulted in higher ultimate pH and R-values of chicken breast than pre-rigor salting with NaCl (P < 0.05). Despite the high ultimate pH, pre-rigor salting with KCl resulted in lower protein solubility, final yield, and hardness of chicken breast than pre-rigor salting with NaCl (P < 0.05). These results indicate that pre-rigor salting with KCl could contribute to the maintenance of relatively excellent technological properties of pre-rigor chicken breasts compared to post-rigor salted chicken breast. However, this current study also suggests that the impact of KCl on technological properties in pre-rigor chicken breast, such as water-holding capacity, protein solubility, and texture, could be less effective than pre-rigor salting with NaCl at an identical percentage concentration.


Assuntos
Carne/análise , Músculos Peitorais/fisiologia , Cloreto de Potássio/administração & dosagem , Animais , Galinhas , Manipulação de Alimentos
10.
AJNR Am J Neuroradiol ; 41(4): 663-668, 2020 04.
Artigo em Inglês | MEDLINE | ID: mdl-32165365

RESUMO

BACKGROUND AND PURPOSE: Flow-diverter treatment for previously stented aneurysms has been reported to be less effective and prone to complications. In this study, we evaluated the effectiveness and safety of flow diverters for recurrent aneurysms after stent-assisted coiling. MATERIALS AND METHODS: Patients who underwent flow-diverter placement for recurrent aneurysms after stent-assisted coiling between March 2015 and March 2019 were recruited. Clinical and radiographic characteristics and clinical and angiographic outcomes were retrospectively evaluated. RESULTS: Among 133 patients who underwent flow-diverter insertion, 17 (male/female ratio = 5:12; mean age, 53.8 years) were treated for recurrent aneurysms after stent placement with (n = 16) or without (n = 1) coiling. Eight patients initially presented with subarachnoid hemorrhage; 7, with headache; and 2, with visual field defects. Angiographic morphology included large/giant saccular in 12 patients, dissecting in 2, fusiform in 1, traumatic pseudoaneurysm in 1, and ruptured blood blister-like aneurysm in 1. The duration between the first treatment and flow-diverter placement ranged from 2 weeks to 15 months (median, 6 months). Flow-diverter placement was successful in all cases without any complications. All patients had favorable outcomes (mRS, 0-2), without any newly appearing symptoms. Aneurysms were followed up with conventional angiography at least once in 6-18 months. Sixteen aneurysms showed complete occlusion, and 1 aneurysm was enlarged. CONCLUSIONS: Results from this case series investigating flow-diverter placement for recurrent aneurysms after stent-assisted coiling suggested that the procedure is safe and effective. Further study in a larger population may be warranted.


Assuntos
Prótese Vascular , Embolização Terapêutica/métodos , Procedimentos Endovasculares/instrumentação , Aneurisma Intracraniano/terapia , Reoperação/instrumentação , Adulto , Idoso , Embolização Terapêutica/instrumentação , Procedimentos Endovasculares/métodos , Feminino , Humanos , Masculino , Pessoa de Meia-Idade , Recidiva , Estudos Retrospectivos , Stents , Resultado do Tratamento
11.
J Nanosci Nanotechnol ; 9(2): 1501-4, 2009 Feb.
Artigo em Inglês | MEDLINE | ID: mdl-19441556

RESUMO

Conducting polyaniline (PANI) and inorganic Na+-Montmorillonite (Na+-MMT) nanocomposite material was prepared via a microemulsion polymerization method. To observe the morphology of the as-synthesized PANI/Na+-MMT nanocomposite, SEM images were employed. D-spacing of the PANI/Na+-MMT nanocomposite obtained from the XRD was found to become wider than that of pure Na+-MMT particles due to the successful intercalation of PANI into Na+-MMT layers. The fabricated PANI/Na+-MMT nanocomposite based electrorheological (ER) fluid was prepared by dispersing the particles in insulating silicone oil. Dynamic mechanical properties of the ER fluid were investigated via an oscillatory testing method using a vertical oscillation rheometer under an applied electric field, showing typical ER behavior.

12.
Oncogene ; 20(25): 3247-57, 2001 May 31.
Artigo em Inglês | MEDLINE | ID: mdl-11423974

RESUMO

Protein kinase CK2 is a ubiquitous and evolutionarily conserved serine/threonine kinase that is upregulated in many human cancers and can serve as an oncogene in lymphocytes. Recently, we have demonstrated that CK2 potentiates Wnt/beta-catenin signaling in mammary epithelial cells. To determine whether CK2 overexpression contributes to mammary tumorigenesis, we have performed comparative studies of human and rat breast cancer specimens and we have engineered transgenic mice with dysregulated expression of CK2alpha in the mammary gland. We find that CK2 is highly expressed in human breast tumor specimens and in carcinogen-induced rat mammary tumors. Overexpression of CK2alpha in the mammary gland of transgenic mice, under control of the MMTV-LTR, causes hyperplasia and dysplasia of the female mammary gland. Thirty per cent of the female MMTV-CK2alpha transgenic mice develop mammary adenocarcinomas at a median of 23 months of age, often associated with Wnt pathway activation, as evidenced by upregulation of beta-catenin protein. NF-kappaB activation and upregulation of c-Myc also occur frequently. Thus, in mice, rats, and humans, dysregulated expression of CK2 is associated with and is capable of contributing to mammary tumorigenesis. Targeted inhibition of CK2 could be useful in the treatment of breast cancer.


Assuntos
Adenocarcinoma/etiologia , Neoplasias da Mama/etiologia , Transformação Celular Neoplásica , Neoplasias Mamárias Animais/etiologia , Proteínas Serina-Treonina Quinases/biossíntese , Transativadores , Proteínas de Peixe-Zebra , Idade de Início , Animais , Caseína Quinase II , Proteínas do Citoesqueleto/metabolismo , Feminino , Doença da Mama Fibrocística , Humanos , Hiperplasia , Lactação , Glândulas Mamárias Animais/patologia , Neoplasias Mamárias Animais/induzido quimicamente , Vírus do Tumor Mamário do Camundongo/genética , Camundongos , Camundongos Transgênicos , NF-kappa B/metabolismo , Proteínas Serina-Treonina Quinases/genética , Proteínas Proto-Oncogênicas/metabolismo , Proteínas Proto-Oncogênicas c-myc/metabolismo , Transdução de Sinais , Proteínas Wnt , beta Catenina
13.
Int Clin Psychopharmacol ; 10(2): 67-72, 1995 Jun.
Artigo em Inglês | MEDLINE | ID: mdl-7673658

RESUMO

A 6 week double-blind placebo-controlled trial of cyproheptadine augmentation of ongoing haloperidol treatment was conducted in 40 chronic schizophrenic in-patients. Cyproheptadine augmentation, compared to administration of haloperidol with placebo, did not produce a statistically significant improvement in psychotic symptoms. Cyproheptadine augmentation caused significant reduction in the extrapyramidal symptoms, which supports the atypical profile of antipsychotics. As to the neuroendocrinological effect, cyproheptadine augmentation did not reduce the plasma prolactin level but did induce a decrease in the plasma cortisol level. Although long-term follow-up studies are needed to confirm the results, this study suggests that cyproheptadine augmentation may be effective in treating chronic schizophrenic patients who are intolerant of extrapyramidal side effects of conventional antipsychotics.


Assuntos
Ciproeptadina/uso terapêutico , Haloperidol/uso terapêutico , Esquizofrenia/tratamento farmacológico , Adulto , Doença Crônica , Método Duplo-Cego , Quimioterapia Combinada , Feminino , Seguimentos , Humanos , Hidrocortisona/sangue , Masculino , Placebos , Prolactina/sangue , Esquizofrenia/sangue , Fatores de Tempo
14.
Arch Dermatol Res ; 280(1): 45-9, 1988.
Artigo em Inglês | MEDLINE | ID: mdl-3281601

RESUMO

A keratinolytic proteinase (KPase) which is regarded as an important factor in the pathogenesis of dermatophytosis was isolated and purified from Microsporum (M.) canis culture filtrates. Enzyme-linked immunosorbent assay (ELISA) was used to determine the occurrence of circulating antibodies to this enzyme in sera samples from guinea pigs with superficial fungal infections caused by M. canis. Of sera samples from guinea pigs infected with M. canis, 75% were reactive within 10 weeks, however, those ELISA values were relatively low compared with those from guinea pigs immunized with KPase. The presence of circulating antibodies was first detected 2 weeks post inoculation with M. canis, corresponding to the period when the lesions were most severe. The titers of the ELISA antibodies reached a peak at 4 weeks; at that time the lesions had disappeared completely.


Assuntos
Anticorpos Antifúngicos/biossíntese , Dermatomicoses/imunologia , Microsporum/enzimologia , Peptídeo Hidrolases/imunologia , Animais , Anticorpos Antifúngicos/análise , Ensaio de Imunoadsorção Enzimática , Feminino , Cobaias , Masculino , Microsporum/imunologia , Fatores de Tempo
15.
Med Oncol ; 18(1): 95-7, 2001.
Artigo em Inglês | MEDLINE | ID: mdl-11778975

RESUMO

Vinorelbine seems to cause less neurotoxicity than other vinca alkaloids because of its selective activity on mitotic cells over axonal microtubules. Clinical trials report very mild peripheral neurotoxicity with distal paresthesiae and there are no reports of myelopathy in these series of patients. The authors describe a patient who developed a rare event of myelopathy while on vinorelbine for non-small-cell lung cancer.


Assuntos
Antineoplásicos Fitogênicos/efeitos adversos , Paclitaxel/uso terapêutico , Doenças da Medula Espinal/induzido quimicamente , Vimblastina/análogos & derivados , Vimblastina/efeitos adversos , Antineoplásicos Fitogênicos/uso terapêutico , Carcinoma Pulmonar de Células não Pequenas/tratamento farmacológico , Feminino , Humanos , Neoplasias Pulmonares/tratamento farmacológico , Imageamento por Ressonância Magnética , Pessoa de Meia-Idade , Medula Espinal/patologia , Doenças da Medula Espinal/patologia , Vimblastina/uso terapêutico , Vinorelbina
16.
Appl Radiat Isot ; 49(1-2): 73-7, 1998.
Artigo em Inglês | MEDLINE | ID: mdl-9467837

RESUMO

We have prepared 4-[18F]fluoromethylbenzylsulfonate esters as fluoromethylbenzylating agents. These agents are readily prepared by an [18F]fluoride ion displacement of the corresponding bissulfonate esters. The application of these 4-[18F]fluoromethylbenzylsulfonate esters to N-alkylation reaction of spiperone and 1-phenylpiperazine shows that the products 3-N-(4-[18F]fluoromethylbenzyl)spiperone and 1-N-(4-[18F]fluoromethylbenzyl)-4-phenylpiperazine are rapidly produced with high radiochemical yields under a no-carrier-added condition.


Assuntos
Radioisótopos de Flúor/química , Marcação por Isótopo/métodos , Mesilatos/química , Alquilantes/química , Alquilação , Piperazinas/química , Espiperona/química
18.
Br J Cancer ; 92(8): 1581-7, 2005 Apr 25.
Artigo em Inglês | MEDLINE | ID: mdl-15798764

RESUMO

As gastrin may play a role in the pathophysiology of gastrointestinal (GI) malignancies, the elucidation of the mechanisms governing gastrin-induced proliferation has recently gained considerable interest. Several studies have reported that a large percentage of colorectal tumours overexpress or stabilise the beta-catenin oncoprotein. We thus sought to determine whether gastrin might regulate beta-catenin expression in colorectal tumour cells. Amidated gastrin-17 (G-17), one of the major circulating forms of gastrin, not only enhanced beta-catenin protein expression, but also one of its target genes, cyclin D1. Furthermore, activation of beta-catenin-dependent transcription by gastrin was confirmed by an increase in LEF-1 reporter activity, as well as enhanced cyclin D1 promoter activity. Finally, G-17 prolonged the tau(1/2) of beta-catenin protein, demonstrating that gastrin appears to exert its mitogenic effects on colorectal tumour cells, at least in part, by stabilising beta-catenin.


Assuntos
Neoplasias Colorretais/metabolismo , Proteínas do Citoesqueleto/metabolismo , Gastrinas/farmacologia , Transativadores/metabolismo , Animais , Northern Blotting , Western Blotting , Linhagem Celular Tumoral , Ciclina D1/efeitos dos fármacos , Ciclina D1/metabolismo , Proteínas do Citoesqueleto/efeitos dos fármacos , Camundongos , Regiões Promotoras Genéticas/efeitos dos fármacos , Transativadores/efeitos dos fármacos , Transcrição Gênica/efeitos dos fármacos , beta Catenina
19.
J Am Acad Dermatol ; 19(6): 1095-101, 1988 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-2462577

RESUMO

A 29-year-old man had a 2-year history of multiple, asymptomatic, firm, subcutaneous nodules on the scrotal skin, which sometimes discharged a chalky material. Fifty-one nodules were observed in the histopathologic examination, which revealed, in addition to the typical findings of idiopathic calcinosis of the scrotum, various forms of intact cysts: epidermal (some calcified), pilar (calcified), hybrid (calcified), and indeterminate cysts with diffusely calcified keratinous content and attenuated walls. A mixture of calcified keratinous material and inflammatory infiltrates was detected, with or without remnants of the cyst wall. These findings suggest that idiopathic calcinosis of the scrotum derives from the dystrophic calcification of cysts.


Assuntos
Calcinose/patologia , Escroto/patologia , Adulto , Calcinose/cirurgia , Cisto Epidérmico/patologia , Doenças dos Genitais Masculinos/patologia , Doenças dos Genitais Masculinos/cirurgia , Humanos , Queratinas/metabolismo , Masculino
20.
J Biol Chem ; 275(31): 23790-7, 2000 Aug 04.
Artigo em Inglês | MEDLINE | ID: mdl-10806215

RESUMO

Protein kinase CK2 (formerly casein kinase II) is a serine/threonine kinase overexpressed in many human tumors, transformed cell lines, and rapidly proliferating tissues. Recent data have shown that many cancers involve inappropriate reactivation of Wnt signaling through ectopic expression of Wnts themselves, as has been seen in a number of human breast cancers, or through mutation of intermediates in the Wnt pathway, such as adenomatous polyposis coli or beta-catenin, as described in colon and other cancers. Wnts are secreted factors that are important in embryonic development, but overexpression of certain Wnts, such as Wnt-1, leads to proliferation and transformation of cells. We report that upon stable transfection of Wnt-1 into the mouse mammary epithelial cell line C57MG, morphological changes and increased proliferation are accompanied by increased levels of CK2, as well as of beta-catenin. CK2 and beta-catenin co-precipitate with the Dvl proteins, which are Wnt signaling intermediates. A major phosphoprotein of the size of beta-catenin appears in in vitro kinase reactions performed on the Dvl immunoprecipitates. In vitro translated beta-catenin, Dvl-2, and Dvl-3 are phosphorylated by CK2. The selective CK2 inhibitor apigenin blocks proliferation of Wnt-1-transfected cells, abrogates phosphorylation of beta-catenin, and reduces beta-catenin and Dvl protein levels. These results demonstrate that endogenous CK2 is a positive regulator of Wnt signaling and growth of mammary epithelial cells.


Assuntos
Células Epiteliais/metabolismo , Glândulas Mamárias Animais/metabolismo , Proteínas Serina-Treonina Quinases/metabolismo , Proteínas Proto-Oncogênicas/metabolismo , Transativadores , Proteínas de Peixe-Zebra , Proteínas Adaptadoras de Transdução de Sinal , Animais , Apigenina , Caseína Quinase II , Linhagem Celular , Transformação Celular Neoplásica , Proteínas do Citoesqueleto/metabolismo , Proteínas Desgrenhadas , Células Epiteliais/citologia , Flavonoides/farmacologia , Glândulas Mamárias Animais/citologia , Camundongos , Mitógenos/metabolismo , Modelos Genéticos , Fosfoproteínas/metabolismo , Fosforilação , Ligação Proteica , Proteínas/metabolismo , Proteínas Proto-Oncogênicas/genética , Transdução de Sinais , Transfecção , Regulação para Cima , Proteínas Wnt , Proteína Wnt1 , beta Catenina
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