The degradation of dissolved organic matter in black and odorous water by humic substance-mediated Fe(II)/Fe(III) cycle under redox fluctuation.

In nature, the hydroxyl radical (•OH) is produced during the anaerobic-aerobic transition when groundwater level fluctuates. In addition, the •OH is also detected in iron-bearing clay minerals and iron oxides during the redox process. Goethite is one of the most stable iron oxides involved in biogeochemical cycles. In this study, the coexisting humic acid (HA) enhanced the generation of Fe(II) during the iron reduction process and accelerated the generation of •OH in the redox process of goethite. The organic contaminants in black and odorous water were decomposed by constructing an iron-reducing bacteria-HA-Fe(II)/Fe(III) reaction system under anaerobic-aerobic alternation. The results demonstrated that in the anaerobic stage, HA could promote the reduction and dissolution of goethite through the complexation effect and electron shuttle mechanism, as well as significantly strengthening the iron reduction process in water. Under aerobic conditions, Fe(II) in the reaction system would activate O2 to generate •O2-. The •OH, formed by Fe (II) and •O2- via Fenton reaction and Haber-Weiss mechanism, oxidized dissolved organic matter (DOM) in water. The characterization of DOM by three-dimensional fluorescence spectroscopy (3DEEM) indicated that after four redox fluctuations, the organic contaminants in water samples were effectively degraded. Generally, this study provides new approaches and insights into the biogeochemical cycling of Fe and C elements and water pollution remediation at the anoxic-anoxic interface.

The performance of co-immobilized strains isolated from activated sludge combined with Scenedesmus quadricauda to remove nutrients and organics in black odorous water.

In this study, three bacteria were isolated from activated sludge (Pseudomonas aeruginosa, Bacillus subtilis, and Dietzia maris). After that, isolated strains and Scenedesmus quadricauda that could degrade refractory organics, as co-immobilization species, were prepared gel beads to treat black odorous water. Under the optimized conditions, the removal rate of chemical oxygen demand (COD), ammonia nitrogen (NH3-N), total nitrogen (TN) and total phosphorus (TP) reached 94.36%, 95.7%, 91.22% and 95.27%, respectively, and organics (including aromatic proteins and microbial-by-product-like compounds) were also significantly removed. Microbial analysis reveals that the community structure had a significant difference before and after treatment, and the main dominant at the genus level was transformed from Nitrospirillum (approximately 18.03%) to Flavobacterium (approximately 17.64%). This study also found that the immobilized gel beads have excellent stability and reusability, which provided a feasible and robust bioremediation strategy for the treatment of actual black-odor water.

Effects of light source and inter-species mixed culture on the growth of microalgae and bacteria for nutrient recycling and microalgae harvesting using black odorous water as the medium.

To achieve the sustainable and effective removal efficiency of nutrients in black odorous water, light source, inter-species microalgae mixed culture, and the harvesting effect were all explored. The results showed that under a LED light source, the addition of interspecific soluble algal products (SAP) promoted the growth of Haematococcus pluvialis (H. pluvialis) M1, and its maximum specific growth rate was 1.76 times that of H. pluvialis cultivated alone. That was due to the hormesis effect between the two kinds of microalgae, the SAP produced by Scenedesmus could stimulate the growth of H. pluvialis. The algae and bacteria symbiotic system with black odorous water as the medium showed excellent performance to treat nutrients, where the concentration of ammonia nitrogen (NH3-N) and total phosphorus (TP) (0.84, 0.23 mg/L) met the requirements of landscape water. The microbial diversity analysis revealed that the introduction of microalgae changed the dominant species of the bacterial community from Bacteroidota to Proteobacteria. Furthermore, timely microalgae harvesting could prevent water quality from deteriorating and was conducive to microalgae growth and resource recycling. The higher harvest efficiency (98.1%) of H. pluvialis was obtained when an inoculation size of 20% and 0.16 g/L FeCl3 were provided.