NLRP3 inflammasome: a promising target in ischemic stroke.

BACKGROUND: Recently, several studies have demonstrated that the NLRP3 inflammasome participates in detecting cellular damage and mediating inflammatory responses to aseptic tissue injury following cerebral ischemia. More importantly, blocking or inhibiting NLRP3 inflammasome at multiple levels, such as its expression, assembly and activity, may offer substantial promise to salvage neurological deterioration during ischemic stroke. However, the specific mechanisms about the contribution of NLRP3 to neurovascular damage remain to be established. MATERIALS AND METHODS: In this paper, we will review the molecular structure, expression and assembly of NLRP3 inflammasome, and illustrate its possible roles and effects in ischemic stroke. Moreover, we will speculate its activity and mechanism in stroke pathogenesis, and present the recent advances and challenges in potential therapies targeting NLRP3 inflammsome. RESULTS AND CONCLUSION: Mounting evidence has demonstrated that NLRP3 inflammasome plays a prominent role in the pathogenesis and progression of ischemic stroke, which indicates the higher possibility to target NLRP3 inflammasome in future stroke therapy. However, many aspects of the biology of NLRP3 inflammasome to stroke are still not well defined or even completely unknown. As the mechanistic insight of the NLRP3 inflammasomes increases, opportunities to develop new therapeutic strategies for patients with ischemic stroke are expected to enhance proportionately.

Salivary IgG4 Levels Contribute to Assessing the Efficacy of Dermatophagoides pteronyssinus Subcutaneous Immunotherapy in Children with Asthma or Allergic Rhinitis.

At present, there are no effective, non-invasive, and objective indicators to evaluate the efficacy of pediatric house dust mite (HDM)-specific allergen immunotherapy (AIT). A prospective, observational study was performed in children with Dermatophagoides pteronyssinus (Der p) asthma and/or allergic rhinitis (AR). Forty-four patients received subcutaneous Der p-AIT for 2 years, and eleven patients received only symptomatic treatment. The patients needed to finish their questionnaires at each visit. Serum and salivary Der p-specific IgE, IgG4, and IgE-blocking factors (IgE-BFs) were measured at 0, 4, 12, and 24 months during AIT. A correlation between them was also evaluated. Subcutaneous Der p-specific AIT improved the clinical symptoms of children with asthma and/or AR. The Der p-specific IgE-BF significantly increased at 4, 12, and 24 months after AIT treatment. Serum and salivary Der p-specific IgG4 significantly increased with the time of the AIT treatment, and significant correlations between them at different time points were observed (p < 0.05). Furthermore, there were significant correlations (R = 0.31-0.62) between the serum Der p-specific IgE-BF and Der p-specific IgG4 at the baseline, 4, 12, and 24 months after the AIT (p < 0.01). The salivary Der p-specific IgG4 levels also demonstrated a certain correlation with the Der p-specific IgE-BF. Der p-specific AIT is an effective treatment for children with asthma and/or AR. Its effect was associated with increased serum and salivary-specific IgG4 levels, as well as an increased IgE-BF. Non-invasive salivary-specific IgG4 may be useful for monitoring the efficacy of AIT in children.

Sensing Shallow Structure and Traffic Noise with Fiber-optic Internet Cables in an Urban Area.

Abstract: Distributed acoustic sensing (DAS) is a novel seismic observation system developed in recent years that can realize ultrahigh density observations and has attracted extensive attention in the field of seismology. DAS uses fiber-optic cables as sensing units, which are easy to incorporate with urban telecommunication fiber-optic cables for seismological observations. Compared with seismometers, DAS has the advantages of being rapidly deployed and recyclable, being able to acquire dense observations at low cost, and convenient data collection. In this study, a 5.2 km long telecom fiber-optic internet cable was utilized as a DAS array in an urban area to record ambient noise, and the noise cross-correlation function (NCF) was calculated. There are two different distribution types of ambient noise sources along the cable, regular along-road trucks (Taihe Road) and complex ambient noise, including human activities and traffic sources along and across the Jinniu road. In the first case, we constructed a 2D S-wave velocity model down to 100 m depth and a low-velocity zone was revealed. The S-wave model well explained the traffic signal along the Taihe road and the low-velocity zone is also consistent with the results obtained from co-located geophone arrays. In the second case, due to the complexity of the traffic noise distribution, empirical Green's functions were barely achieved. Therefore, we performed a synthetic test obtaining different NCFs with different source distributions, and two specific cases that dominate the NCF results were matched. Finally, we obtained the traffic noise distribution along the road, which is consistent with the power spectra density of the ambient noise. In conclusion, by combining DAS and urban fiber-optic internet cables with urban traffic noise, we can effectively reveal the traffic activities and image shallow structures with high resolution, which could offer a reference for urban construction and disaster prevention. Article Highlights: DAS turns the urban fiber-optic internet cables into ultra-dense permanent seismic observation arraysWe revealed a high-resolution shallow structure using urban fiber-optic internet cablesWe obtained the distribution of traffic activities along the road.

The Effects and Underlying Mechanisms of Cell Therapy on Blood-Brain Barrier Integrity After Ischemic Stroke.

Ischemic stroke is one of the main causes of mortality and disability worldwide. However, efficient therapeutic strategies are still lacking. Stem/progenitor cell-based therapy, with its vigorous advantages, has emerged as a promising tool for the treatment of ischemic stroke. The mechanisms involve new neural cells and neuronal circuitry formation, antioxidation, inflammation alleviation, angiogenesis, and neurogenesis promotion. In the past decades, in-depth studies have suggested that cell therapy could promote vascular stabilization and decrease blood-brain barrier (BBB) leakage after ischemic stroke. However, the effects and underlying mechanisms on BBB integrity induced by the engrafted cells in ischemic stroke have not been reviewed yet. Herein, we will update the progress in research on the effects of cell therapy on BBB integrity after ischemic stroke and review the underlying mechanisms. First, we will present an overview of BBB dysfunction under the ischemic condition and cells engraftment for ischemic treatment. Then, we will summarize and discuss the current knowledge about the effects and underlying mechanisms of cell therapy on BBB integrity after ischemic stroke. In particular, we will review the most recent studies in regard to the relationship between cell therapy and BBB in tissue plasminogen activator (t-PA)-mediated therapy and diabetic stroke.