RESUMO
HupB is an iron-regulated protein in Mycobacterium tuberculosis that functions as a positive regulator of mycobactin biosynthesis. It is essential for the growth and survival of the pathogen inside macrophages. Previously, using the full-length rHupB of M. tuberculosis, we demonstrated high levels of anti-HupB antibodies in the serum of pulmonary tuberculosis (TB) and, interestingly, extrapulmonary TB patients with negligible levels in household contacts and healthy controls. Here, we used three antigenic fragments of HupB, namely the recombinant HupB-F1 (aa 1-71), HupB-F2 (aa 63-161) and HupB-F3 (aa 164-214), as antigens in enzyme-linked immunosorbent assay (ELISA) to screen serum from TB patients. HupB-F2 showed enhanced immunoreactivity with serum from patients with pulmonary TB (three groups consisting of new cases, defaulters and recurrent cases) and extrapulmonary TB, with negligible levels in normal healthy controls. The negative correlation of the anti-(HupB-F2) antibodies with serum iron was maximal, with a Pearson's correlation coefficient value of -0.415. The study, in addition to strengthening the diagnostic potential of HupB, reflected the superior performance of HupB-F2 as an antigen in screening pulmonary and extrapulmonary TB.
Assuntos
Anticorpos Antibacterianos/sangue , Antígenos de Bactérias , Proteínas de Bactérias , Testes Diagnósticos de Rotina/métodos , Histonas , Mycobacterium tuberculosis/imunologia , Tuberculose/diagnóstico , Adulto , Idoso , Ensaio de Imunoadsorção Enzimática/métodos , Feminino , Humanos , Masculino , Programas de Rastreamento/métodos , Pessoa de Meia-Idade , Proteínas Recombinantes , Testes Sorológicos/métodosRESUMO
Aneurysms are rare in children. Isolated iliac artery aneurysms are very rare, especially bilateral aneurysms. Pediatric aneurysms are usually secondary to connective tissue disorders, arteritis, or mycotic causes. We present a case of a 3-year-old child with bilateral idiopathic common iliac aneurysms that were successfully repaired with autogenous vein grafts.
Assuntos
Veia Femoral/transplante , Aneurisma Ilíaco/cirurgia , Procedimentos Cirúrgicos Vasculares , Fatores Etários , Pré-Escolar , Humanos , Aneurisma Ilíaco/diagnóstico , Masculino , Tomografia Computadorizada por Raios X , Resultado do TratamentoRESUMO
Arteriovenous malformations are notorious for their propensity to bleed, sometimes with fatal consequences. We describe an unusual case of a patient with polycystic kidney disease and with a large arteriovenous malformation involving the orbit and scalp who presented with multiple episodes of profuse bleeding from the upper eyelid and loss of vision in the corresponding eye.