RESUMO
Based on similarities in basidial morphology and nucleotide sequences of the V3 variable region in the large sub-unit ribosomal RNA, the yeast Leucosporidium lari-marini is considered phylogenetically related to the genus Cystofilobasidium. Therefore the new combination Cystofilobasidium lari-marini is proposed.
Assuntos
Basidiomycota/classificação , RNA Fúngico/química , RNA Ribossômico/química , Leveduras/classificação , Basidiomycota/genética , Alinhamento de Sequência , Análise de Sequência de RNA , Leveduras/genéticaRESUMO
The validation of Sporidiobolus microsporus Higham, nom. nud. is based on phenotypic characterization and molecular sequence analysis of a partial region of the large sub-unit ribosomal DNA. The species is compared, based on phenotypic and molecular characteristics, with other species of Sporidiobolus and the closely related Rhodosporidium fluviale.
Assuntos
Basidiomycota/classificação , DNA Ribossômico/genética , Dados de Sequência Molecular , Técnicas de Tipagem Micológica , Fenótipo , RNA Ribossômico/genética , Reprodutibilidade dos Testes , Análise de Sequência de DNARESUMO
The V3 variable region of the large subunit rRNA was examined for nucleotide sequence signatures as potential taxonomic tools. Data are presented on 117 species, representing 23 genera of basidiomycetous yeasts. The results of nucleotide sequence alignments indicate that strains within species have identical base sequences and that species may differ from one another by one to more than 100 base positions. Phylogenetic analyses of the alignments indicates relationships among species, including the prediction of synonymous species and the clustering of species belonging to the Ustilaginales and Tremellales. These results suggest that species-specific nucleotide sequences can be used for the development of techniques for population analyses of a variety of marine and other microeukaryotes.
Assuntos
RNA Fúngico/química , RNA Ribossômico/química , Microbiologia da Água , Leveduras/classificação , Leveduras/genética , Animais , Sequência de Bases , Humanos , Dados de Sequência Molecular , Filogenia , RNA Fúngico/isolamento & purificação , RNA Ribossômico/isolamento & purificação , Água do Mar , Alinhamento de Sequência , Homologia de Sequência do Ácido NucleicoRESUMO
The molecular systematics of 337 strains of basidiomycetous yeasts and yeast-like fungi, representing 230 species in 18 anamorphic and 24 teleomorphic genera, was determined by sequence analysis of the D1/D2 region of the large-subunit rDNA. The data were compared with published sequences of other basidiomycetous fungi. The results demonstrated that the yeast species and genera are phylogenetically distributed among the Microbotryum, Sporidiobolus, Agaricostilbum and Erythrobasidium clades of the Urediniomycetes; the Tremellales, Trichosporonales ord. nov., Filobasidiales and Cystofilobasidiales clades of the Hymenomycetes; and the Ustilaginales, Microstromatales and Malasseziales clades of the Ustilaginomycetes. Genera such as Bensingtonia, Cryptococcus, Rhodotorula and Sporobolomyces are polyphyletic, i.e. they occur in two or more clades. In contrast, other genera, e.g. Bullera, Cystofilobasidium, Fellomyces, Filobasidiella, Filobasidium, Kondoa, Kurtzmanomyces, Leucosporidium, Rhodosporidium, Sporidiobolus and Udeniomyces, are monophyletic. The majority of the species can be identified using D1/D2 analyses, although the internal transcribed spacer region is required to distinguish closely related species. The intergenic spacer region is recommended for additional differentiation of species and strains.