RESUMO
Squamous carcinomas of both human and rodent origin can undergo a transition to a more invasive, metastatic phenotype involving reorganization of the cytoskeleton, loss of cell adhesion molecules such as E-cadherin and acquisition of a fibroblastoid or spindle cell morphology. We have developed a series of cell lines from mouse skin tumors which represent different stages of carcinogenesis, including benign papillomas, and clonally related squamous and spindle carcinomas derived from the same primary tumor. Some spindle cells continue to express keratins, but with a poorly organized keratin filament network, whereas in others no keratin expression is detectable. All of the spindle cells lack expression of the cell adhesion molecule E-cadherin and the desmosomal component desmoplakin. Loss of these cell surface proteins therefore appears to precede the destabilization of the keratin network. At the genetic level, it is not known whether such changes involve activation of dominantly acting oncogenes or loss of a suppressor function which controls epithelial differentiation. To examine this question, we have carried out a series of fusion experiments between a highly malignant mouse skin spindle cell carcinoma and cell lines derived from premalignant or malignant mouse skin tumors, including both squamous and spindle carcinoma variants. The results show that the spindle cell phenotype as determined by cell morphology and lack of expression of keratin, E-cadherin, and desmoplakin proteins, is recessive in all hybrids with squamous cells. The hybrids expressed all of these differentiation markers, and showed suppression of tumorigenicity to a variable level dependent upon the tumorigenic properties of the less malignant fusion partner. Our results suggest that acquisition of the spindle cell phenotype involves functional loss of a gene(s) which controls epithelial differentiation.
Assuntos
Carcinoma de Células Escamosas/patologia , Carcinoma/patologia , Transformação Celular Neoplásica/genética , Transformação Celular Neoplásica/patologia , Genes Recessivos/genética , Neoplasias Cutâneas/patologia , Animais , Antígenos de Diferenciação/análise , Antígenos de Diferenciação/genética , Caderinas/análise , Caderinas/genética , Carcinoma/química , Carcinoma/ultraestrutura , Carcinoma de Células Escamosas/química , Carcinoma de Células Escamosas/ultraestrutura , Proteínas do Citoesqueleto/análise , Proteínas do Citoesqueleto/genética , Citoesqueleto/química , Citoesqueleto/ultraestrutura , Desmoplaquinas , Epiderme/química , Epiderme/patologia , Epiderme/ultraestrutura , Imunofluorescência , Regulação da Expressão Gênica/genética , Genes Supressores/genética , Genes Supressores/fisiologia , Células Híbridas/química , Imuno-Histoquímica , Queratinas/análise , Queratinas/genética , Camundongos , Fenótipo , Neoplasias Cutâneas/química , Neoplasias Cutâneas/ultraestrutura , Células Tumorais CultivadasRESUMO
Chemical contamination of aquatic systems often co-occurs with dramatic changes in surrounding terrestrial vegetation. Plant leaf litter serves as a crucial resource input to many freshwater systems, and changes in litter species composition can alter the attributes of freshwater communities. However, little is known how variation in litter inputs interacts with chemical contaminants. We investigated the ecological effects resulting from changes in tree leaf litter inputs to freshwater communities, and how those changes might interact with the timing of insecticide contamination. Using the common insecticide malathion, we hypothesized that inputs of nutrient-rich and labile leaf litter (e.g., elm [Ulmus spp.] or maple [Acer spp.]) would reduce the negative effects of insecticides on wetland communities relative to inputs of recalcitrant litter (e.g., oak [Quercus spp.]). We exposed artificial wetland communities to a factorial combination of three litter species treatments (elm, maple, and oak) and four insecticide treatments (no insecticide, small weekly doses of 10 µg L-1, and either early or late large doses of 50 µg L-1). Communities consisted of microbes, algae, snails, amphipods, zooplankton, and two species of tadpoles. After two months, we found that maple and elm litter generally induced greater primary and secondary production. Insecticides induced a reduction in the abundance of amphipods and some zooplankton species, and increased phytoplankton. In addition, we found interactive effects of litter species and insecticide treatments on amphibian responses, although specific effects depended on application regime. Specifically, with the addition of insecticide, elm and maple litter induced a reduction in gray tree frog survival, oak and elm litter delayed tree frog metamorphosis, and oak and maple litter reduced green frog tadpole mass. Our results suggest that attention to local forest composition, as well as the timing of pesticide application might help ameliorate the harmful effects of pesticides observed in freshwater systems.
Assuntos
Monitoramento Ambiental , Inseticidas/análise , Áreas Alagadas , Acer/efeitos dos fármacos , Animais , Anuros , Ecologia , Ecossistema , Água Doce , Larva/efeitos dos fármacos , Malation/toxicidade , Fitoplâncton/efeitos dos fármacos , Folhas de Planta/química , Plantas/efeitos dos fármacos , Quercus/efeitos dos fármacos , Zooplâncton/efeitos dos fármacosRESUMO
Evidences have been reported that phenylacetic (PA) and phenylbutyric (PB) fatty aromatic acids can exert tumor growth inhibition in vitro and in vivo. Moreover, clinical trials also showed some activity for these drugs to modulate the expression of genes implicated in tumor growth, metastasis, immunogenicity, and to potentiate the efficacy of cytotoxic agents. The aim of the study was to examine the effects of PA and PB on the growth as well as sensitization to cisplatin and radiation in human cervical cancer cells. The effects of PA and PB on the proliferative activity and apoptosis induction in cervical tumor tissue was investigated. Both PA and PB exhibited a time- and dose-dependent antiproliferative activity in SW756 and ME180 cell lines: after 72-h treatment, the IC50 (concentration able to inhibit 50% of cell growth) of PB was 1.9 +/- 0.2 mM and 1.5 +/- 0.2 mM in SW756 and ME180 cells, respectively, while the IC50 of PA was 13.0 +/- 1.7 mM and 10.0 +/- 1.2 mM in SW756 and ME180 cells, respectively. In tumor tissue biopsies obtained from patients affected by squamous cervical cancer, both drugs resulted in a marked reduction of the percentage of bromodeoxyuridine-labeled cells compared with untreated samples [19.0 +/- 1.63% in untreated tissues with respect to 1.30 +/- 0.54% and 4.20 +/- 2.50% of stained cells after treatment with PA (30 mM) (P < 0.0001) and PB (5 mM) (P < 0.0001), respectively]. Moreover, analysis of the staining with M30 monoclonal antibody revealed that PA (30 mM) and PB (5 mM) were able to produce a marked increase in the number of stained apoptotic nuclei with respect to untreated samples. Finally, PB and PA were shown to enhance the sensitivity of SW756 to radiation and to exert an additive effect when combined with cisplatin. A significant reduction of the processed form of p21ras and rhoB proteins in the membrane fraction of cells exposed to PA and PB was observed. When farnesol, which is able to circumvent the enzymatic step inhibited by PA and PB, was added to the medium only a partial reversal of the growth inhibition and potentiation of sensitivity to radiation induced by PA and PB were found. In conclusion, the growth inhibitory properties of fatty aromatic acids suggest that these molecules could represent the prototype of a new class of compounds with some therapeutic potential in cervical cancer.
Assuntos
Ácidos Graxos/farmacologia , Radiossensibilizantes/uso terapêutico , Neoplasias do Colo do Útero/tratamento farmacológico , Neoplasias do Colo do Útero/radioterapia , Antimetabólitos Antineoplásicos/uso terapêutico , Antineoplásicos/uso terapêutico , Apoptose , Bromodesoxiuridina/farmacologia , Ciclo Celular , Divisão Celular , Cisplatino/uso terapêutico , Terapia Combinada , Fragmentação do DNA , Relação Dose-Resposta a Droga , Relação Dose-Resposta à Radiação , Feminino , Humanos , Concentração Inibidora 50 , Queratinas/metabolismo , Fenilacetatos/farmacologia , Fenilbutiratos/farmacologia , Proteínas Proto-Oncogênicas p21(ras)/metabolismo , Fatores de Tempo , Células Tumorais Cultivadas , Proteína rhoB de Ligação ao GTP/metabolismoAssuntos
Transformação Celular Neoplásica/genética , Neoplasias Cutâneas/genética , Animais , Transformação Celular Neoplásica/metabolismo , Modelos Animais de Doenças , Genes Supressores de Tumor/fisiologia , Camundongos , Neoplasias Cutâneas/fisiopatologia , Fator de Crescimento Transformador beta/fisiologiaRESUMO
Cancer can be considered a genetic disorder of somatic cells. Strong evidence comes from several areas: (1) chromosomal analysis reveals cancer cells have abnormal karyotypes; (2) some inherited syndromes are associated with an increased risk of cancer and for others, cancer itself occurs as an inherited trait; (3) cells can become malignant by a variety of agents that damage DNA, and (4) some types of viruses can induce tumours. One common thread has been the normal cellular sequences transduced by viruses and mutated to become oncogenic (oncogenes) are the same sequences to become activated by nonviral mechanisms. These oncogenes appear involved in cell proliferation and/or differentiation and their products apparently function in the signal transduction pathway from the cell exterior to the nucleus. In addition, evidence from familial studies indicate mutations associated with gene inactivation or loss of function are also important in the aetiology of tumour formation. These genes, termed tumour suppressor genes, seem to be involved in the negative control of cellular proliferation. Cancer is a multistep process and it is now becoming clear that the different stages involve genetic alterations in both oncogenes and tumour suppressor genes.
Assuntos
Genes Supressores de Tumor , Neoplasias/genética , Oncogenes , Humanos , Transdução de SinaisRESUMO
The ultimate stage of carcinogenesis in both human and mouse epithelial cells is the ability to invade surrounding tissues and metastasize to distant sites. In mouse skin tumours, the development of the invasive, spindle cell phenotype is associated with an imbalance of alleles on mouse chromosome 7, including the H-ras gene. In previous work, we have described clonally related squamous and spindle cell lines from the same primary tumour which differed substantially in morphology and behaviour, but showed the same series of mutations in H-ras and p53 genes. One of the events which takes place during this transition is disruption of cell-cell contacts, possibly due to the induced expression of metalloproteinases such as stromelysin-1 and disappearance of the cell adhesion molecule E-cadherin. Parallel studies using somatic cell hybrids have shown that the spindle cell phenotype is recessive in hybrids between squamous and spindle cells. We propose that an important epidermal differentiation-controlling gene is lost during the spindle cell transition.
Assuntos
Neoplasias Cutâneas/patologia , Neoplasias Cutâneas/secundário , Animais , Carcinoma/genética , Carcinoma/patologia , Carcinoma/secundário , Progressão da Doença , Camundongos , Invasividade Neoplásica , Fenótipo , Neoplasias Cutâneas/genética , Células Tumorais CultivadasRESUMO
Studies of multistage carcinogenesis in mouse skin have provided many of the early concepts of tumour initiation, promotion and progression. Genetic approaches have led to the identification of a number of mutational alterations in proto-oncogenes and tumour suppressor genes which take place at specific stages of carcinogenesis in this particular system. Initiation involves, at least in a proportion of tumours, mutational activation of the cellular H-ras proto-oncogene. Trisomy of chromosome 7, which develops during the premalignant clonal expansion phase, possibly as a consequence of tumour promoter treatment, is followed by further alterations on chromosome 7 which lead to a relative increase in the expression of mutant ras alleles. The p53 tumour suppressor gene undergoes mutational alteration and loss of heterozygosity in a proportion of squamous carcinomas but this particular gene does not appear to be involved in the further transition of squamous carcinomas to highly undifferentiated spindle cell tumours. The latter transition appears to be a recessive event which can be complemented by fusion with cells at earlier stages of malignancy. Mouse skin carcinogenesis therefore continues to provide invaluable information on the nature of the genetic and biological transitions which occur during the step-wise progression of normal cells to malignancy.