Self-Motion of Water Droplets along a Spacing Gradient of Micropillar Arrays on Copper.

Self-driven droplet transport along an open gradient surface is increasingly becoming popular for various microfluidics applications. In this work, a gradient copper oxide layer is formed on a copper sheet (as a bipolar electrode, BPE) in a KOH solution by bipolar electrochemistry. The deposits at different positions present a rich variety of colors, compositions, and microstructures along the longitudinal axis of the BPE. More than half the length of the anodic pole is covered by a Cu(OH)2/CuO composite layer of several micrometers thick, which is composed of dense micropillars with a decreasing spacing gradient to the anodic direction. The micropillar arrays are superhydrophilic, and after modified with 1-dodecanethiol, the tops of the dense micropillars constitute a hydrophobic and microscopically discontinuous surface with a wettability gradient. On such a gradient surface water droplets can move spontaneously to more hydrophilic direction at a velocity of about 16 mm s-1. The superhydrophobicity of the modified micropillar arrays is discussed through a comparison with the wax tubules on a lotus leaf. Theoretical analysis of the driving force reveals that the concave surface effect of water at the spacings between the micropillars is the critical factor for driving the rolling motion of the droplets along the gradient micropillar arrays.

Genomics insights into different cellobiose hydrolysis activities in two Trichoderma hamatum strains.

BACKGROUND: Efficient biomass bioconversion is a promising solution to alternative energy resources and environmental issues associated with lignocellulosic wastes. The Trichoderma species of cellulolytic fungi have strong cellulose-degrading capability, and their cellulase systems have been extensively studied. Currently, a major limitation of Trichoderma strains is their low production of ß-glucosidases. RESULTS: We isolated two Trichoderma hamatum strains YYH13 and YYH16 with drastically different cellulose degrading efficiencies. YYH13 has higher cellobiose-hydrolyzing efficiency. To understand mechanisms underlying such differences, we sequenced the genomes of YYH13 and YYH16, which are essentially identical (38.93 and 38.92 Mb, respectively) and are similar to that of the T. hamatum strain GD12. Using GeneMark-ES, we annotated 11,316 and 11,755 protein-coding genes in YYH13 and YYH16, respectively. Comparative analysis identified 13 functionally important genes in YYH13 under positive selection. Through examining orthologous relationships, we identified 172,655, and 320 genome-specific genes in YYH13, YYH16, and GD12, respectively. We found 15 protease families that show differences between YYH13 and YYH16. Enzymatic tests showed that exoglucanase, endoglucanase, and ß-glucosidase activities were higher in YYH13 than YYH16. Additionally, YYH13 contains 10 families of carbohydrate-active enzymes, including GH1, GH3, GH18, GH35, and GH55 families of chitinases, glucosidases, galactosidases, and glucanases, which are subject to stronger positive selection pressure. Furthermore, we found that the ß-glucosidase gene (YYH1311079) and pGEX-KG/YYH1311079 bacterial expression vector may provide valuable insight for designing ß-glucosidase with higher cellobiose-hydrolyzing efficiencies. CONCLUSIONS: This study suggests that the YYH13 strain of T. hamatum has the potential to serve as a model organism for producing cellulase because of its strong ability to efficiently degrade cellulosic biomass. The genome sequences of YYH13 and YYH16 represents a valuable resource for studying efficient production of biofuels.

The Central Role of Nitrogen Atoms in a Zeolitic Imidazolate Framework-Derived Catalyst for Cathodic Hydrogen Evolution.

Platinum usually offers the most effective active center for hydrogen evolution reaction (HER), because of the optimal trade-off between the adsorption and desorption of hydrogeN atoms (H*) on Pt atoms. Herein, we report an unusual result regarding the active center of a HER catalyst, which was synthesized by electrodepositing traces of Pt nanoparticles (NPs) into a porous nitrogen-rich dodecahedron matrix derived from zeolitic imidazolate framework ZIF-8. With an ultra-low Pt loading of 2.76 µg cm-2 , the N-Pt-bonded catalyst can produce a current density of 117 mA cm-2 for the HER in 1.0 m H2 SO4 at an overpotential of 50 mV, whereas the commercial Pt/C (300 µg cm-2 Pt) can only reach 50 mA cm-2 under the same conditions. Cyclic voltammetry demonstrates that both the H* adsorption and the Pt oxidation are not allowed to occur on this catalyst, due to a full surface coverage of the trace Pt NPs by imidazole. The results from the specially designed experiments indicate that the imidazole N atoms may act as proton anchor-sites for the HER due to their electron donor nature. Density functional theory calculations also support a catalytic HER mechanism centered at the Pt-supported N active center, which needs a Gibbs free energy of H* absorption (ΔGH* ) significantly smaller than the absolute value of ΔGH* on the Pt(111) surface. We hope that the results of this study will encourage the research on novel N-centered catalysts for the HER.

Brassinolide Increases Potato Root Growth In Vitro in a Dose-Dependent Way and Alleviates Salinity Stress.

Brassinosteroids (BRs) are steroidal phytohormones that regulate various physiological processes, such as root development and stress tolerance. In the present study, we showed that brassinolide (BL) affects potato root in vitro growth in a dose-dependent manner. Low BL concentrations (0.1 and 0.01 µg/L) promoted root elongation and lateral root development, whereas high BL concentrations (1-100 µg/L) inhibited root elongation. There was a significant (P < 0.05) positive correlation between root activity and BL concentrations within a range from 0.01 to 100 µg/L, with the peak activity of 8.238 mg TTC·g-1 FW·h-1 at a BL concentration of 100 µg/L. Furthermore, plants treated with 50 µg/L BL showed enhanced salt stress tolerance through in vitro growth. Under this scenario, BL treatment enhanced the proline content and antioxidant enzymes' (superoxide dismutase, peroxidase, and catalase) activity and reduced malondialdehyde content in potato shoots. Application of BL maintain K+ and Na+ homeostasis by improving tissue K+/Na+ ratio. Therefore, we suggested that the effects of BL on root development from stem fragments explants as well as on primary root development are dose-dependent and that BL application alleviates salt stress on potato by improving root activity, root/shoot ratio, and antioxidative capacity in shoots and maintaining K+/Na+ homeostasis in potato shoots and roots.