RESUMO
Effects of some glutamate receptor antagonists on signal propagation elicited by stimulation of association fibers in guinea pig piriform cortex slices were investigated using optical imaging. During simultaneous application of both NMDA and non-NMDA receptor antagonists (D-2-amino-5-phosphonopentanoic acid and 6-cyano-7-nitroquinoxaline-2,3-dione, respectively) the postsynaptic activity was largely suppressed, and a weak although distinct late propagation component was found to survive. This latter component was reversibly suppressed by application of low Ca(2+) solution or a group I/II specific metabotropic glutamate receptor (mGluR) antagonist (+)-alpha-methyl-4-carboxyphenylglycine. These results suggest that mGluRs mediate postsynaptic excitation, which would play a crucial role in activating the reverberating positive feedback circuit effectively.
Assuntos
Córtex Cerebral/fisiologia , Antagonistas de Aminoácidos Excitatórios/farmacologia , Potenciais Pós-Sinápticos Excitadores/efeitos dos fármacos , Receptores de Glutamato Metabotrópico/fisiologia , Animais , Córtex Cerebral/efeitos dos fármacos , Potenciais Pós-Sinápticos Excitadores/fisiologia , Feminino , Cobaias , Técnicas In Vitro , Receptores de Glutamato Metabotrópico/antagonistas & inibidoresRESUMO
Metabotropic glutamate receptors (mGluRs) were previously shown to mediate a postsynaptic late propagation component elicited by layer Ib stimulation in guinea pig piriform cortex slices. In the present study, the effects of some group specific or subtype specific mGluR antagonists on the late propagation component were investigated using an optical imaging method, in order to identify mGluR subtypes mediating it. A selective mGluR1 antagonist (RS)-1-aminoindan-1,5-dicarboxylic acid most effectively suppressed the late component whereas a selective mGluR5 antagonist, selective group II or group III antagonists showed little or no suppressive effect. These results suggest that the late propagation component is mediated by mGluR1.