First Report of Sweet Potato Virus E(SPVE) Infecting Sweet Potato in China.

Sweet potato (Ipomoea batatas [L.] Lam.) is a versatile crop, cultivated in the subtropical and tropical areas, as food, fodder, and industrial raw material crop. In China, sweet potato has been used as a health-care food in recent years, as it contains a wide range of nutrients and xenobiotic phytochemicals. However, viral diseases are major constraint for the sweet potato yield and quality, especially the seed production and quality. Over 30 species of viruses infect sweet potato worldwide (Clark et al. 2012). More recently, a few new viruses infected sweet potato were identified, such as sweet potato virus E (SPVE), which was reported in Korea(Jo et al. 2020). In May 2022, a sweet potato sample (JSXZ1) with virus-like symptom, such as mosaic and vein clearing were collected from sweet potato germplasm Xuzhou resource nursery, Jiangsu Province, China (N34˚16', E117˚18') (Fig. S1A). To investigate the virus disease, the sample JSXZ1 showing the typical symptoms of disease was prepared for Small-RNA (sRNA) deep-sequencing. The sRNA library was constructed using TruSeq™ Small RNA Sample Prep Kits (Illumina, San Diego, USA) and sequenced using the Illumine Hiseq 2500 platform by LC-Bop Technologies (Hangzhou) CO., LTD. The sample was sequenced to obtain 26, 358, 439 raw reads and 22, 969, 139 clean reads after quality control trimming and analysis. The Velvet 1.0.5 software was used to de novo assemble the clean reads (18 to 28 nt) into larger contigs, which were then compared with the nucleotide sequences in the National Center for Biotechnology Information (NCBI) database using the BLASTn algorithm. Viruses found in the sample were sweet potato latent virus (SPLV), sweet potato feathery mottle virus (SPFMV), sweet potato chlorotic stunt virus (SPCSV), sweet potato badnavirus A (SPBV-A) and sweet potato badnavirus B (SPBV-B). Surprisingly, besides the viruses listed above, 28 contigs matched sequences of SPVE isolate GS (MH388502). To verify the result, total RNA was extracted from the sample JSXZ1 and from other leave samples (JSXZ2-JSXZ5) that contained SPFMV, SPVC, SPLV, SPVG respectively stored in lab using FastPure Universal Plant Total RNA Isolation Kit (Vazyme Biotech Co., LTD, Nanjing, China). cDNA was synthesized using random primer (hexadeoxyribonucleotide mixture; pd(N)6). The cDNA serves as template in PCR using a newly designed primer pairs based on SPVE p1 gene (SPVE-F: 5'- TCACCAAAAAGAATGCTACAAC-3'/SPVE-R: 5'-GAAATCCTCCCACTCTCCATA-3'). An expected ~500-bp PCR fragment was obtained in JSXZ1, while none of the fragment was obtained from JSXZ2-JSXZ5 (Fig. S1B). The PCR fragment was cloned into pMD18-T vector (Takara Bio Inc., Beijing, China) and plasmid DNA from transformed Escherichia coli DH5α cell (n=3) were commercially sequenced by Sangon Biotech (Shanghai) Co., Ltd. The sequences of the three fragment clones we obtained were 100% identical when compared. A BLASTN analysis of the sequences revealed that they are specific to SPVE and shared 98.62% nucleotide identity to SPVE GS isolate (MH388502) and one sequence was submitted to GenBank (Accession number OQ948331). To determine the occurrence of SPVE in infected sweet potato plants, a total of 37 leaves samples with viral symptom collected from Shandong Province (n=6) and Jiangsu Province (n=31) were indexed by RT-PCR as described before. Only 9 (24.3%) out of 37 from Shandong (n=1) and Jiangsu (n=8) were positive to SPVE respectively. In addition, five additional viruses (SPFMV, SPVC, SPVG, SPLV, SPCSV) were detected among these 37 samples and always in a mixed infection of two or more viruses. To our knowledge, this is the first report of SPVE infecting sweet potato in China. Sweet potato is an important crop in China and other countries (Zhang et al. 2023). China is the largest sweet potato producer all over the world. In addition, as sweet potato is produced through the vegetative propagation mode, thus, more attention should be paid to detection and monitoring of occurrence of SPVE in China.

Transmissive Pancharatnam-Berry metasurfaces with stable amplitude and precise phase modulations using dartboard discretization configuration.

Metasurfaces are ultra-thin artificial structures capable of flexibly manipulating electromagnetic (EM) waves. Among various applications, phase modulation of electromagnetic (EM) waves using metasurfaces holds great significance. The Pancharatnam-Berry (P-B) metasurfaces provides a complete 2π phase modulation by simply rotating the meta-atom. However, the fixed lattice in rotation employed by traditional P-B metasurfaces often results in unstable amplitude and imprecise P-B phase, leading to performance degradation. In this work, we demonstrate transmissive P-B metasurfaces with stable amplitude and precise phase modulation. To ensure stable amplitude and precise P-B phase, we adopt a dartboard discretization configuration with a hexagonal lattice for the meta-atom design. By applying topology optimization to the encoding sequence formed by surface pixels and dimensions, we significantly enhancing the high transmissive bandwidth of the optimized meta-atom. Furthermore, the optimized meta-atom exhibits a stable amplitude and precise P-B phase for each rotation angle. As proof-of-concept demonstrations, two metasurfaces for single and multiplexed vortex beams generating are designed utilizing the optimized meta-atom. Both the simulated and measured results indicate high mode purity of generated vortex beams. The design method can also be readily extended to other high performance metasurfaces with stable amplitude and precise phase manipulations, which can enhance the efficiency and capacity of metasurface-assisted holographic imaging and 6 G wireless communication systems.

Rapid design of hybrid mechanism metasurface with random coding for terahertz dual-band RCS reduction.

In this paper, a hybrid mechanism metasurface (HMM) employing 1-bit random coding is proposed to achieve polarization-insensitive and dual-wideband monostatic/bistatic radar cross section (RCS) reduction under a wide range of incident angles. The anisotropic unit cell is designed by the combination of the multi-objective particle swarm optimization (MOPSO) algorithm and Python-CST joint simulation, which facilitates the rapid acquisition of the desired unit cell with excellent dual-band absorption conversion capability. The unit cell and its mirrored version are used to represent the units "0" and "1", respectively. In addition, the array distribution with random coding of the units "0" and "1" is optimized under different incident angles, polarizations and frequencies, which enables better diffusion-like scattering. Simulation results demonstrate that the proposed coding HMM can effectively reduce the monostatic/bistatic RCS by over 10 dB within the dual-band frequency ranges of 2.07-3.02 THz and 3.78-4.71 THz. Furthermore, the specular and bistatic RCS reduction performances remain stable at oblique incident angles up to 45° for both TE and TM polarizations.

Non-Abelian Inverse Anderson Transitions.

Inverse Anderson transitions, where the flat-band localization is destroyed by disorder, have been wildly investigated in quantum and classical systems in the presence of Abelian gauge fields. Here, we report the first investigation on inverse Anderson transitions in the system with non-Abelian gauge fields. It is found that pseudospin-dependent localized and delocalized eigenstates coexist in the disordered non-Abelian Aharonov-Bohm cage, making inverse Anderson transitions depend on the relative phase of two internal pseudospins. Such an exotic phenomenon induced by the interplay between non-Abelian gauge fields and disorder has no Abelian analogy. Furthermore, we theoretically design and experimentally fabricate non-Abelian Aharonov-Bohm topolectrical circuits to observe the non-Abelian inverse Anderson transition. Through the direct measurements of frequency-dependent impedance responses and voltage dynamics, the pseudospin-dependent non-Abelian inverse Anderson transitions are observed. Our results establish the connection between inverse Anderson transitions and non-Abelian gauge fields, and thus comprise a new insight on the fundamental aspects of localization in disordered non-Abelian flat-band systems.

First Report of Meloidogyne enterolobii Infecting Solanum nigrum in China.

Black nightshade (Solanum nigrum) typically grows as a weed species, but it is also widely used as an herb to treat stomach ulcers and dermal infections in many countries (Jabamalairaj et al. 2019). In April 2023, extensive root galls similar to those associated with by root-knot nematodes (RKNs), Meloidogyne spp., were observed on the roots of black nightshade in several commercial fields in Lufeng county (22°55'57.44″N, 115°33'10.31″E), Guangdong Province, China. Upon inspection, there were one to several female RKN in each gall, and egg masses protruding through the root surface. The disease incidence rate was more than 90% in each field using the random sampling method. The nematode population densities in the samples ranged from 279 to 656 eggs and second-stage juveniles (J2s) per gram of fresh roots. Females and egg masses were collected from the roots, and egg masses were incubated in sterile water at 25°C to obtain J2s. Males were not collected in root galling or soil samples. The J2 tail is thin with a broad, bluntly pointed tip, and a clearly defined hyaline tail terminus. Measurements of J2 (n = 20) included: L= 440 ± 30.5 (384 to 500) µm, stylet = 12.3 ± 0.7 (11.3 to 13.7) µm, tail = 51.6 ± 2.4 (47.9 to 57.0) µm. For females (n = 15), vulval slit length = 25.5 ±1.9 (23.6 to 29.1) µm, vulval slit to anus distance = 22.1 ± 3.0 (18.2 to 27.0) µm. Stylet knobs in females are divided longitudinally by a groove so that each knob appears as two. The perineal patterns are round to ovoid, with coarse and smooth striae, moderate to high dorsal arch and mostly lacking distinct lateral lines. Morphological characteristics from J2s and perineal patterns from adult females fit the original description of M. enterolobii (Yang and Eisenback 1983). Furthermore, species identity was explored by sequencing the D2-D3 region of the 28S rRNA gene using primers D2A/D3B (Vrain et al. 1992), and the mtDNA cytochrome c oxidase I (COI) genes using primers JB3/JB5 (Derycke et al. 2005). The sequences for the target genes were 759 bp (GenBank Accession No. OR046056) and 447 bp (GenBank Accession No. OR042802), respectively. The BLAST analysis suggested 98.17~99.78% similarities to other available M. enterolobii sequences in GenBank. Species identity was further confirmed with the species-specific primer pair Me-F/Me-R (Long et al. 2006). An approximately 240 bp PCR product was produced, which was previously reported only for M. enterolobii, whereas no product was obtained from control populations of M. incognita or M. javanica. The pathogenicity test was conducted in a greenhouse at 28°C using seedlings of S. nigrum maintained in pots containing 500 cm3 sterilized soil. Ten replicates were inoculated with 800 eggs and J2s of the original population of M. enterolobii, while another 10 replicates of control plants were not inoculated. After 7 weeks, the inoculated plants exhibited galling symptoms similar to plants observed in the field, and females and egg masses were obtained by dissecting galls. No galling symptoms were observed on control plants. These results confirmed the nematode's pathogenicity. To our knowledge, this is the first record of M. enterolobii parasitizing black nightshade. M. enterolobii stands out as a highly deleterious variant among the species of RKNs owing to its extensive repertoire of host plants, pathogenicity, and proficiency in thriving and multiplying even on crops possessing resistance genes (Sikandar, 2022). In addition to being a medicinal plant, S. nigrum is a widespread weed found in fields throughout China. This report also showed that S. nigrum could play an important role as a reservoir host of M. enterolobii aiding its survival, reproduction, spread, and increasing the potential damage for host crops.

Micro-Motion Extraction for Marine Targets by Multi-Pulse Delay Conjugate Multiplication and Layered Tracking.

The detection and recognition of marine targets can be improved by utilizing the micro-motion induced by ocean waves. However, distinguishing and tracking overlapping targets is challenging when multiple extended targets overlap in the range dimension of the radar echo. In this paper, we propose a multi-pulse delay conjugate multiplication and layered tracking (MDCM-LT) algorithm for micro-motion trajectory tracking. The MDCM method is first applied to obtain the conjugate phase from the radar echo, which enables high-precision micro-motion extraction and overlapping state identification of extended targets. Then, the LT algorithm is proposed to track the sparse scattering points belonging to different extended targets. In our simulation, the root mean square errors of the distance and velocity trajectories were better than 0.277 m and 0.016 m/s, respectively. Our results demonstrate that the proposed method has the potential to improve the precision and reliability of marine target detection through radar.

IbINV Positively Regulates Resistance to Black Rot Disease Caused by Ceratocystis fimbriata in Sweet Potato.

Black rot disease, caused by Ceratocystis fimbriata Ellis & Halsted, severely affects both plant growth and post-harvest storage of sweet potatoes. Invertase (INV) enzymes play essential roles in hydrolyzing sucrose into glucose and fructose and participate in the regulation of plant defense responses. However, little is known about the functions of INV in the growth and responses to black rot disease in sweet potato. In this study, we identified and characterized an INV-like gene, named IbINV, from sweet potato. IbINV contained a pectin methylesterase-conserved domain. IbINV transcripts were most abundant in the stem and were significantly induced in response to C. fimbriata, salicylic acid, and jasmonic acid treatments. Overexpressing IbINV in sweet potato (OEV plants) led to vigorous growth and high resistance to black rot disease, while the down-regulation of IbINV by RNA interference (RiV plants) resulted in reduced plant growth and high sensitivity to black rot disease. Furthermore, OEV plants contained a decreased sucrose content and increased hexoses content, which might be responsible for the increased INV activities; not surprisingly, RiV plants showed the opposite effects. Taken together, these results indicate that IbINV positively regulates plant growth and black rot disease resistance in sweet potato, mainly by modulating sugar metabolism.

Topology optimization of the azimuth-rotation-independent polarization conversion metasurface for bandwidth enhancement.

Metasurfaces offer an unprecedented opportunity for flexible manipulation of electromagnetic wave. The azimuth-rotation-independent (ARI) polarization conversion metasurface (PCM) is an ultrathin device, which could convert an arbitrary linearly-polarized incident wave to its cross-polarized state. However, the bandwidth of an ARI PCM with a high cross-polarized transmission is usually limited. Here, a topology optimization method of multi-feature points based on the differential evolution (DE) algorithm is adopted to enhance the bandwidth of the traditional ARI PCM while maintaining a high transmission and polarization conversion ratio. The simulated results of the optimized structure indicate a 2.08 times bandwidth expansion in the cross-polarization conversion compared with the original structure. In addition, the measured results are consistent with the simulated ones and the ARI characteristic is validated. The proposed method provides a promising route for efficient high-performance metasurface designs.

Phase-to-pattern inverse design for a fast realization of a functional metasurface by combining a deep neural network and a genetic algorithm.

Metasurface provides an unprecedented means to manipulate electromagnetic waves within a two-dimensional planar structure. Traditionally, the design of meta-atom follows the pattern-to-phase paradigm, which requires a time-consuming brute-forcing process. In this work, we present a fast inverse meta-atom design method for the phase-to-pattern mapping by combining the deep neural network (DNN) and genetic algorithm (GA). The trained classification DNN with an accuracy of 92% controls the population generated by the GA within an arbitrary preset small phase range, which could greatly enhance the optimization efficiency with less iterations and a higher accuracy. As proof-of-concept demonstrations, two reflective functional metasurfaces including an orbital angular momentum generator and a metalens have been numerically investigated. The simulated results agree very well with the design goals. In addition, the metalens is also experimentally validated. The proposed method could pave a new avenue for the fast design of the meta-atoms and functional meta-devices.

Development of a dual RT-RPA detection for Sweet potato feathery mottle virus and Sweet potato chlorotic stuntvirus.

The disease co-infected by Sweet potato feathery mottle virus (SPFMV) and Sweet potato chlorotic stunt virus (SPCSV) is devastating in sweet potato, as it would give rise to the serious losses in both production and quality. Consequently, it is conducive for preventing and controlling this disease to detect these two viruses accurately and timely. Here we developed and optimized a dual reverse transcription recombinase polymerase amplification (RT-RPA) for rapid and accurate detection of SPFMV and SPCSV. Four special primers were designed based on the conserved sequences of SPFMV and SPCSV, respectively. The sensitivity of dual RT-RPA for SPFMV and SPCSV was 10-4 ng/µL at the optimal conditions in which the primer ratio between SPFMV and SPCSV was 2:1, and the reaction incubated for 25 min at a temperature of 39 °C. Both 61 sweet potato samples and 5 morning glory samples collected from China were tested using the dual RT-RPA successfully. Therefore, the dual RT-RPA is a reliable, rapid, sensitive method to detect these two viruses in sweet potato. It is the RT-RPA that was used for detection of SPFMV and SPCSV simultaneously firstly. This dual RT-RPA, as a convenient and powerful tool, will be useful to diagnose SPFMV and SPCSV.

Velocity measurement of an arbitrary three-dimensional moving object by using a novel modulated field.

The rotational Doppler effect caused by vortex beam carrying orbital angular momentum is recently used to estimate the rotational velocity of the object. However, the vortex beam only has the spiral phase distribution in one dimension, which means that only the rotational movement of the object would introduce the frequency shift. Also, the vortex beam has a spatial amplitude distribution of doughnut-shaped, which is not suitable for many application scenarios. To simultaneously measure the velocity of an arbitrary three-dimensional moving object, we propose theoretically and demonstrate experimentally an effective method by constructing a novel modulated field. Different from the plane wave and the vortex beam, the modulated field has linear phase distribution in azimuth and elevation directions. In addition, the modulated field has the maximal radiation intensity in the center, which avoids the beam divergence of the vortex beam. By decomposing the frequency shift caused by the radial, azimuth and elevation movements, we realize the velocity measurement in three dimensions. Experiments in a microwave system show that the estimated velocity errors are lower than 6.0%.

Experimental Observation of Higher-Order Topological Anderson Insulators.

Recently, a new family of symmetry-protected higher-order topological insulators has been proposed and was shown to host lower-dimensional boundary states. However, with the existence of the strong disorder in the bulk, the crystal symmetry is broken, and the associated corner states are disappeared. It is well known that the emergence of robust edge states and quantized transport can be induced by adding sufficient disorders into a topologically trivial insulator, that is the so-called topological Anderson insulator. The question is whether disorders can also cause the higher-order topological phase. This is not known so far, because interactions between disorders and the higher-order topological phases are completely different from those with the first-order topological system. Here, we demonstrate theoretically that the disorder-induced higher-order topological corner state and quantized fraction corner charge can appear in a modified Haldane model. In experiments, we construct the classical analog of such higher-order topological Anderson insulators using electric circuits and observe the disorder-induced corner state through the voltage measurement. Our work defies the conventional view that the disorder is detrimental to the higher-order topological phase, and offers a feasible platform to investigate the interaction between disorders and higher-order topological phases.

An Impedance-Loaded Orthogonal Frequency-Coded SAW Sensor for Passive Wireless Sensor Networks.

A passive wireless impedance-loaded orthogonal frequency-coded (OFC) surface acoustic wave (SAW) sensor for wireless sensor networks was proposed in this paper. One of the chips on OFC SAW tag is connected to an external sensor, which could cause a phase shift in the time response of the corresponding part on the SAW device. The phase shift corresponds to the sensed quantity, which could be temperature, strain, vibration, pressure, etc. The OFC SAW tag is isolated by a proper package from the direct effect of the measurand on the device's response which could avoid the multiple measurands coupling. The simultaneous work of multiple sensors is guaranteed by orthogonal frequency coding. By processing the response based on an extended matched filter algorithm, sensing information of the specific coded OFC device can be extracted from the superimposed response of multiple independent encoded sensors. Compared to previous methods, the proposed method can produce a more flexible passive (battery-free) wireless sensor suitable for large-scale wireless sensor networks. Simulation and experimental results demonstrate the effectiveness of the sensor.

Quantum-inspired microwave signal processing for implementing unitary transforms.

Inspired by photonic one-way quantum computation, we describe a microwave signal processing method for implementing unitary transforms based on measuring the cebits encoded in the "classical microwave graph state (CMGS)." Here the terms "cebit" and "CMGS" defined in our system are classical analogies of a qubit and certain target quantum graph states in quantum physics respectively, which can exhibit some similar behaviors and resultants. The constructions of 4- and 16-cebit CMGSs as examples are discussed in detail and specific tomography methods are introduced to characterize their qualities. By performing operations on these CMGSs, we implement some basic 2 × 2, 4 × 4, and specific generalized unitary transforms, and obtain output results with high fidelities. Furthermore, we also demonstrate that a simulation of an efficient Grover's search algorithm, which has been executed in one-way quantum computing schemes, can be directly realized via a certain 4-cebit CMGS. Due to the excellent parallel efficiency and credible outcomes in the proposal, this quantum-inspired method may provide benefits for exploring new ways to microwave information processing, or in turn as an alternative tool for simulating particular quantum systems to some extent.

Obtaining source current density related to irregularly structured electromagnetic target field inside human body using hybrid inverse/FDTD method.

Inverse method is inherently suitable for calculating the distribution of source current density related with an irregularly structured electromagnetic target field. However, the present form of inverse method cannot calculate complex field-tissue interactions. A novel hybrid inverse/finite-difference time domain (FDTD) method that can calculate the complex field-tissue interactions for the inverse design of source current density related with an irregularly structured electromagnetic target field is proposed. A Huygens' equivalent surface is established as a bridge to combine the inverse and FDTD method. Distribution of the radiofrequency (RF) magnetic field on the Huygens' equivalent surface is obtained using the FDTD method by considering the complex field-tissue interactions within the human body model. The obtained magnetic field distributed on the Huygens' equivalent surface is regarded as the next target. The current density on the designated source surface is derived using the inverse method. The homogeneity of target magnetic field and specific energy absorption rate are calculated to verify the proposed method.

Novel Multiresistant Osmotin-like Protein from Sweetpotato as a Promising Biofungicide to Control Ceratocystis fimbriata by Destroying Spores through Accumulation of Reactive Oxygen Species.

Osmotin-like proteins (OLPs) play an important role in host-plant defense. In this study, a novel multiresistant OLP (IbOLP1) was screened from sweetpotato (Ipomoea batatas) with a molecular weight of 26.3 kDa. The expression level of IbOLP1 was significantly higher in resistant cultivars than susceptible ones after inoculation with Ceratocystis fimbriata, which causes black rot disease in sweetpotato. The expression of IbOLP1 in Pichia pastoris led to the lysis of yeast cells themselves. The recombinant IbOLP1 displayed antifungal, antibacterial, and antinematode activity and stability. IbOLP1 could restrain the mycelial growth and lyse spores of C. fimbriata, distinctly reducing the incidence of black rot in sweetpotato. The IbOLP1 can trigger the apoptosis of black rot spores by elevating the intracellular levels of reactive oxygen species. Collectively, these findings suggest that IbOLP1 can be used to develop natural antimicrobial resources instead of chemical agents and generate new, disease-resistant germplasm.

Hyperbolic band topology with non-trivial second Chern numbers.

Topological band theory establishes a standardized framework for classifying different types of topological matters. Recent investigations have shown that hyperbolic lattices in non-Euclidean space can also be characterized by hyperbolic Bloch theorem. This theory promotes the investigation of hyperbolic band topology, where hyperbolic topological band insulators protected by first Chern numbers have been proposed. Here, we report a new finding on the construction of hyperbolic topological band insulators with a vanished first Chern number but a non-trivial second Chern number. Our model possesses the non-abelian translational symmetry of {8,8} hyperbolic tiling. By engineering intercell couplings and onsite potentials of sublattices in each unit cell, the non-trivial bandgaps with quantized second Chern numbers can appear. In experiments, we fabricate two types of finite hyperbolic circuit networks with periodic boundary conditions and partially open boundary conditions to detect hyperbolic topological band insulators. Our work suggests a new way to engineer hyperbolic topological states with higher-order topological invariants.

Non-Hermitian Topolectrical Circuit Sensor with High Sensitivity.

Electronic sensors play important roles in various applications, such as industry and environmental monitoring, biomedical sample ingredient analysis, wireless networks and so on. However, the sensitivity and robustness of current schemes are often limited by the low quality-factors of resonators and fabrication disorders. Hence, exploring new mechanisms of the electronic sensor with a high-level sensitivity and a strong robustness is of great significance. Here, a new way to design electronic sensors with superior performances based on exotic properties of non-Hermitian topological physics is proposed. Owing to the extreme boundary-sensitivity of non-Hermitian topological zero modes, the frequency shift induced by boundary perturbations can show an exponential growth trend with respect to the size of non-Hermitian topolectrical circuit sensors. Moreover, such an exponential growth sensitivity is also robust against disorders of circuit elements. Using designed non-Hermitian topolectrical circuit sensors, the ultrasensitive identification of the distance, rotation angle, and liquid level is further experimentally verified with the designed capacitive devices. The proposed non-Hermitian topolectrical circuit sensors can possess a wide range of applications in ultrasensitive environmental monitoring and show an exciting prospect for next-generation sensing technologies.

Transcriptome Characterization and Gene Changes Induced by Fusarium solani in Sweetpotato Roots.

Sweetpotato (Ipomoea batatas) is an important root crop that is infected by Fusarium solani in both seedling and root stages, causing irregular black or brown disease spots and root rot and canker. This study aims to use RNA sequencing technology to investigate the dynamic changes in root transcriptome profiles between control check and roots at 6 h, 24 h, 3 days, and 5 days post-inoculation (hpi/dpi) with F. solani. The results showed that the defense reaction of sweetpotato could be divided into an early step (6 and 24 hpi) without symptoms and a late step to respond to F. solani infection (3 and 5 dpi). The differentially expressed genes (DEGs) in response to F. solani infection were enriched in the cellular component, biological process, and molecular function, with more DEGs in the biological process and molecular function than in the cellular component. Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analysis showed that the main pathways were metabolic pathways, the biosynthesis of secondary metabolites, and carbon metabolism. More downregulated genes were identified than upregulated genes in the plant-pathogen interaction and transcription factors, which might be related to the degree of host resistance to F. solani. The findings of this study provide an important basis to further characterize the complex mechanisms of sweetpotato resistance against biotic stress and identify new candidate genes for increasing the resistance of sweetpotato.