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1.
BMC Cancer ; 19(1): 988, 2019 Oct 23.
Artigo em Inglês | MEDLINE | ID: mdl-31647032

RESUMO

BACKGROUND: Laparoscopic surgery, fast-track perioperative treatment and XELOX chemotherapy are effective strategies for shortening the duration of hospital stay for cancer patients. This trial aimed to clarify the safety and efficacy of the fast-track multidisciplinary treatment (FTMDT) model compared to conventional surgery combined with chemotherapy in Chinese colorectal cancer patients. METHODS: This trial was a prospective randomized controlled study with a 2 × 2 balanced factorial design and was conducted at six hospitals. Patients in group 1 (FTMDT) received fast-track perioperative treatment and XELOX adjuvant chemotherapy. Patients in group 2 (conventional treatment) received conventional perioperative treatment and mFOLFOX6 adjuvant chemotherapy. Subgroups 1a and 2a had laparoscopic surgery and subgroups 1b and 2b had open surgery. The primary endpoint was total length of hospital stay during treatment. RESULTS: A total of 374 patients were randomly assigned to the four subgroups, and 342 patients were finally analyzed, including 87 patients in subgroup 1a, 85 in subgroup 1b, 86 in subgroup 2a, and 84 in subgroup 2b. The total hospital stay of group 1 was shorter than that of group 2 [13 days, (IQR, 11-17 days) vs. 23.5 days (IQR, 15-42 days), P = 0.0001]. Compared to group 2, group 1 had lower surgical costs, fewer in-hospital complications and faster recovery (all P < 0.05). Subgroup 1a showed faster surgical recovery than that of subgroup 1b (all P < 0.05). There was no difference in 5-year overall survival between groups 1 and 2 [87.1% (95% CI, 80.7-91.5%) vs. 87.1% (95% CI, 80.8-91.4%), P = 0.7420]. CONCLUSIONS: The FTMDT model, which integrates laparoscopic surgery, fast-track treatment, and XELOX chemotherapy, was the superior model for enhancing the recovery of Chinese patients with colorectal cancer. TRIAL REGISTRATION: ClinicalTrials.gov: NCT01080547 , registered on March 4, 2010.


Assuntos
Protocolos de Quimioterapia Combinada Antineoplásica/uso terapêutico , Neoplasias Colorretais/tratamento farmacológico , Neoplasias Colorretais/cirurgia , Desoxicitidina/análogos & derivados , Fluoruracila/análogos & derivados , Laparoscopia , Idoso , Capecitabina , Quimioterapia Adjuvante , Neoplasias Colorretais/patologia , Custos e Análise de Custo , Desoxicitidina/uso terapêutico , Feminino , Fluoruracila/uso terapêutico , Humanos , Tempo de Internação , Leucovorina/uso terapêutico , Masculino , Pessoa de Meia-Idade , Compostos Organoplatínicos/uso terapêutico , Oxaloacetatos , Estudos Prospectivos , Qualidade de Vida , Resultado do Tratamento
2.
Reprod Fertil Dev ; 31(10): 1597-1606, 2019 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-31142430

RESUMO

Polycystic ovary syndrome (PCOS) is an endocrine disorder with a high prevalence in women of childbearing age. To date, there is no method of efficiently diagnosing PCOS and curing it completely because its pathomechanism remains unclear. Here, we investigated whether metabolic abnormalities maintain the hyperandrogenism and PCOS-like ovaries and whether the symptoms induced by excess androgen are treatable. We ceased the abnormal dihydrotestosterone (DHT) stimulation to determine changes in PCOS-like mice. After ceasing DHT stimulation, the ovarian morphology and gene expression recovered from the DHT-stimulated status. However, after cessation of DHT stimulation, the hypertrophy of adipose tissues and hepatic steatosis were not significantly restored, and fat accumulation-related gene expression and serum metabolic markers in the mice were altered. These findings showed that the reproductive dysfunction was obviously relieved, but because the metabolic abnormalities were not relieved after the cessation of excess androgen for 30 days, it appears that the latter may not maintain the former.


Assuntos
Di-Hidrotestosterona/administração & dosagem , Di-Hidrotestosterona/efeitos adversos , Hiperandrogenismo/induzido quimicamente , Síndrome Metabólica/induzido quimicamente , Síndrome Metabólica/patologia , Síndrome do Ovário Policístico/induzido quimicamente , Tecido Adiposo/efeitos dos fármacos , Tecido Adiposo/patologia , Animais , Modelos Animais de Doenças , Progressão da Doença , Feminino , Expressão Gênica/efeitos dos fármacos , Hiperandrogenismo/sangue , Hiperandrogenismo/genética , Síndrome Metabólica/sangue , Síndrome Metabólica/genética , Camundongos , Camundongos Endogâmicos C57BL , Ovário/efeitos dos fármacos , Ovário/patologia , Síndrome do Ovário Policístico/sangue , Síndrome do Ovário Policístico/genética , Estimulação Química , Fatores de Tempo
3.
Reprod Biol Endocrinol ; 16(1): 50, 2018 May 24.
Artigo em Inglês | MEDLINE | ID: mdl-29793502

RESUMO

BACKGROUND: Polycystic ovary syndrome (PCOS) is a complex genetic disease with multifarious phenotypes. Many researches use dehydroepiandrosterone (DHEA) to induce PCOS in pubertal mouse models. The aim of this study was to investigate the role of GPR1 in dehydroepiandrosterone (DHEA)-induced hyperandrogenized mice. METHODS: Prepubertal C57BL/6 mice (25 days of age) and Gpr1-deficient mice were each divided into two groups and injected daily with sesame oil with or without DHEA (6 mg/100 g) for 21 consecutive days. Hematoxylin and eosin (H&E) staining was performed to determine the characteristics of the DHEA-treated ovaries. Real-time PCR was used to examine steroid synthesis enzymes gene expression. Granulosa cell was cultured to explore the mechanism of DHEA-induced, GPR1-mediated estradiol secretion. RESULTS: DHEA treatment induced some aspects of PCOS in wild-type mice, such as increased body weight, elevated serum testosterone, increased number of small, cystic, atretic follicles, and absence of corpus luteum in ovaries. However, Gpr1 deficiency significantly attenuated the DHEA-induced weight gain and ovarian phenotype, improving steroidogenesis in ovaries and estradiol synthesis in cultured granulosa cells, partially through mTOR signaling. CONCLUSIONS: In conclusion, Gpr1 deficiency leads to the improvement of steroid synthesis in mice hyperandrogenized with DHEA, indicating that GPR1 may be a therapeutic target for DHEA-induced hyperandrogenism.


Assuntos
Hiperandrogenismo/sangue , Hiperandrogenismo/genética , Receptores Acoplados a Proteínas G/genética , Testosterona/sangue , Animais , Células Cultivadas , Desidroepiandrosterona , Modelos Animais de Doenças , Estradiol/sangue , Feminino , Hiperandrogenismo/induzido quimicamente , Hiperandrogenismo/patologia , Camundongos , Camundongos Endogâmicos C57BL , Camundongos Knockout , Síndrome do Ovário Policístico/sangue , Síndrome do Ovário Policístico/induzido quimicamente , Síndrome do Ovário Policístico/genética , Síndrome do Ovário Policístico/patologia
4.
Int J Radiat Oncol Biol Phys ; 119(3): 884-895, 2024 Jul 01.
Artigo em Inglês | MEDLINE | ID: mdl-38185388

RESUMO

PURPOSE: The aim of this work was to determine whether locally advanced rectal cancer (LARC) with negative mesorectal fascia (MRF) predicted by magnetic resonance imaging (MRI) can be excluded from preoperative radiation therapy treatment. METHODS AND MATERIALS: This multicenter, open-label, non-inferiority, randomized clinical trial enrolled patients with LARC within 6 to 12 cm from the anal verge and with negative MRI-predicted MRF. Participants were randomized to the intervention group (primary surgery, in which the patients with positive pathologic [CRM] circumferential margins were subjected to chemoradiotherapy [CRT] and those with negative CRM underwent adjuvant chemotherapy according to pathologic staging) or the control group (preoperative CRT, in which all patients underwent subsequent surgery and adjuvant chemotherapy). The primary endpoint was 3-year disease-free survival (DFS). RESULTS: A total of 275 patients were randomly assigned to the intervention (n = 140) and control (n = 135) groups, in which 33.57% and 28.15% patients were at clinical T4 stage and 85.92% and 80.45% patients were at "bad" or "ugly" risk in the intervention and control groups, respectively. There were 2 patients (1.52%) and 1 patient (0.77%) with positive CRM in the intervention and control groups, respectively (P > .05). The non-adherence rates for the intervention and control groups were 3.6% and 23.7%, respectively. After a median follow-up of 34.6 months (IQR, 18.2-45.7), 43 patients had positive events (28 patients and 15 patients in the intervention and control groups, respectively). There were 6 patients (4.4%) with local recurrence in the intervention group and none in the control group, which led to the termination of the trial. The 3-year DFS rate was 81.82% in the intervention group (95% CI, 78.18%-85.46%) and 85.37% in the control group (95% CI, 81.75%-88.99%), with a difference of -3.55% (95% CI, -3.71% to -3.39%; hazard ratio, 1.76; 95% CI, 0.94-3.30). In the per-protocol data set, the difference between 3-year DFS rates was -5.44% (95% CI, -5.63% to -5.25%; hazard ratio, 2.02; 95% CI, 1.01-4.06). CONCLUSIONS: Based on the outcomes of this trial, in patients with LARC and MRI-negative MRF, primary surgery could negatively influence their DFS rates. Therefore, primary surgery was an inferior strategy compared with preoperative CRT followed by surgery and cannot be recommended for patients with LARC.


Assuntos
Quimiorradioterapia , Neoplasias Retais , Humanos , Neoplasias Retais/terapia , Neoplasias Retais/patologia , Neoplasias Retais/diagnóstico por imagem , Neoplasias Retais/mortalidade , Masculino , Feminino , Pessoa de Meia-Idade , Idoso , Intervalo Livre de Doença , Imageamento por Ressonância Magnética , Adulto , Cuidados Pré-Operatórios , Fáscia/diagnóstico por imagem , Estadiamento de Neoplasias , Quimioterapia Adjuvante
5.
Zhongguo Dang Dai Er Ke Za Zhi ; 14(8): 622-7, 2012 Aug.
Artigo em Chinês | MEDLINE | ID: mdl-22898286

RESUMO

OBJECTIVE: To study the effects of budesonide on hypoxia inducible factor 1α(HIF-1α) and vascular endothelial growth factor (VEGF) expression, angiogenesis and airway remodeling in the chronic asthmatic mouse model. METHODS: Thirty female BALB/c mice were randomly divided into normal control, asthma model and treatment groups (10 in each group).The asthmatic mouse model was established via OVA challenge test. Mice in the treatment group were administered with aerosol budesonide (100 µg/kg) an hour before the OVA challenge test from the 28th day. Mice in the control group were treated with PBS instead of OVA. Hematoxylin and eosin staining was performed to observe thickness of the airway wall. Masson staining was used for examing collagen deposition of lung tissues. Angiogenesis and HIF-1α and VEGF expression were measured using immunohistochemistry and Western blot. The relationship of airway wall thickness and vessel area to HIF-1α and VEGF expression was investigated. RESULTS: Vessel area, collagen deposition of lung tissues and airway wall thickness increased in the asthma model group. Levels of HIF-1α and VEGF were also elevated. Administration of budesonide significantly reduced angiogenesis, collagen deposition of lung tissues and airway wall thickening, as well as expression of HIF-1α and VEGF. The vessel area and airway wall thickness were positively correlated with expression of HIF-1α and VEGF. A positive correlation was also found between the expression of HIF-1α and VEGF. CONCLUSIONS: Budesonide can decease angiogenesis and airway remodeling by inhibiting HIF-1α and VEGF expression in asthmatic mice.


Assuntos
Remodelação das Vias Aéreas/efeitos dos fármacos , Asma/tratamento farmacológico , Broncodilatadores/farmacologia , Budesonida/farmacologia , Subunidade alfa do Fator 1 Induzível por Hipóxia/análise , Fator A de Crescimento do Endotélio Vascular/análise , Animais , Asma/metabolismo , Asma/patologia , Brônquios/patologia , Modelos Animais de Doenças , Feminino , Camundongos , Camundongos Endogâmicos BALB C , Neovascularização Fisiológica
6.
Front Cell Dev Biol ; 9: 669189, 2021.
Artigo em Inglês | MEDLINE | ID: mdl-34109177

RESUMO

Hyperandrogenism is a key pathological feature of polycystic ovarian syndrome (PCOS). Excess androgen can lead to PCOS-like cell hypertrophy in the ovaries and adipose tissue of rodents. Here, we established a dihydrotestosterone (DHT)-induced hyperandrogenic mouse model to analyze the differences in gene expression and signaling pathways of the ovaries and gonad fat pads of mice treated with or without DHT by RNA microarray analysis. From the results, we focused on the overlapping differentially expressed gene-Col6a5-and the major differentially enriched signaling pathway-lipid metabolism. We employed DHT-induced mouse ovarian stromal cell, adipogenic 3T3-L1 cell and hepatic cell line NCTC1469 models to investigate whether androgens directly mediate lipid accumulation and hypertrophy. We found that DHT increased lipid droplet accumulation in ovarian stromal cells and adipogenic 3T3-L1 cells but not NCTC1469 cells. DHT significantly altered stromal cell cholesterol metabolism and steroidogenesis, as indicated by changes in cholesterol levels and the expression of related genes, but these effects were not observed in 3T3-L1 cells. Moreover, Col6a5 expression was significantly increased in ovaries and gonadal fat pads of DHT-treated mice, and Col6a5 inhibition alleviated DHT-induced excess lipid accumulation and hypertrophy of ovarian stromal cells and adipogenic 3T3-L1 cells, even improved lipid metabolism in overnourished NCTC1469 cells. Our results indicate that Col6a5 plays important roles in the pathogenesis of DHT-induced lipid metabolism disorder and the hypertrophy of ovarian stromal cells and adipocytes.

7.
Immunotherapy ; 12(4): 229-234, 2020 03.
Artigo em Inglês | MEDLINE | ID: mdl-32172642

RESUMO

Metastatic colorectal cancer with BRAF mutation is a type of highly invasive malignant tumor with poor prognosis and few treatment options. Here, we report a case of a BRAF-mutant and DNA mismatch-repair deficiency colorectal cancer patient with postoperative recurrence as well as abdominal cavity and pelvic metastasis, whose condition was relieved continuously after treatment with a new anti-PD-1 antibody, BGB-A317.


Assuntos
Anticorpos Monoclonais Humanizados/uso terapêutico , Neoplasias Colorretais/tratamento farmacológico , Neoplasias Colorretais/patologia , Reparo de Erro de Pareamento de DNA/genética , Inibidores de Checkpoint Imunológico/uso terapêutico , Proteínas Proto-Oncogênicas B-raf/genética , Neoplasias Abdominais/tratamento farmacológico , Neoplasias Abdominais/genética , Neoplasias Abdominais/secundário , Adenocarcinoma/tratamento farmacológico , Adenocarcinoma/genética , Adenocarcinoma/secundário , Idoso , Biomarcadores Tumorais/genética , Neoplasias Colorretais/genética , Feminino , Humanos , Imunoterapia , Instabilidade de Microssatélites , Mutação , Neoplasias Pélvicas/tratamento farmacológico , Neoplasias Pélvicas/genética , Neoplasias Pélvicas/secundário , Resultado do Tratamento
8.
J Colloid Interface Sci ; 561: 638-646, 2020 Mar 01.
Artigo em Inglês | MEDLINE | ID: mdl-31744618

RESUMO

HYPOTHESIS: The transition metal phosphide is one of the promising bifunctional electrocatalysts for overall water splitting. Moreover, the activity of phosphide catalysts can be further enhanced by the cationic vacancy engineering. EXPERIMENTS: The self-growth Ni2P nanosheet arrays with abundant cationic vacancy defects (V-Ni2P/NF) has been synthesized via a facile multi-step reaction process involving hydrothermal, phosphorization and acid-etching of Mn which was doped in Ni2P nanosheets as a sacrificial dopant. Furthermore, the experimental studies and density functional theory (DFT) calculations were carried out to evaluate its electrochemical performance. FINDINGS: The chemical and electrocatalytic property of Ni2P were successfully optimized by cationic vacancy engineering and the obtained V-Ni2P/NF catalyst exhibited superior bifunctional catalytic performance for both hydrogen evolution (HER) and oxygen evolution reaction (OER) compared to pristine Ni2P and Mn-doped Ni2P in alkaline electrolyte. The V-Ni2P/NF can afford the current density of 10 mA cm-2 at a small overpotential of 55 mV for HER and 250 mV for OER. Additionally, the water electrolysis device assembled by the V-Ni2P/NF electrode as both the anode and cathode just requires a small voltage of 1.59 V to achieve 10 mA cm-2 and shows no obvious attenuation for 50 h.

9.
Surg Endosc ; 23(4): 854-61, 2009 Apr.
Artigo em Inglês | MEDLINE | ID: mdl-18946703

RESUMO

BACKGROUND: This study aimed to assess the efficacy and safety of laparoscopic resection (LR) for rectal cancer. METHODS: A case-control study involving three Chinese medical centers was conducted. Rectal cancer patients undergoing LR were compared with open resection (OR) cases simultaneously from January 2004 to December 2005. Data were collected, and basic characteristics, conversion rate, recovery, complications, adjuvant therapy, and recurrence rate were compared. Analysis was by intention to treat. RESULTS: A total of 335 rectal cancer procedures (115 LR and 220 OR) met the inclusion criteria. The patients' basic characteristics were similar in the two groups (p>0.05). Total mesorectal excision was performed for 85.59% of the patients (201/235), who received anal sphincter preservation. Compared with OR, LR had a shorter incision length, less blood loss, and less need for transfusion, but the operation time was longer (p<0.05). No significant differences were observed between the two groups in positive rates of longitudinal resection margins, numbers of harvested lymph nodes, complication rates during operation and postoperation, and perioperative reoperation and morbidity rates (p>0.05). Postoperative parenteral narcotics were used less in LR than in OR (47.8% vs 62.7%; chi(2)=6.867; p=0.009). The median time until first flatus; resumption of diet, defecation, micturition, and ambulation; and discharge were reduced in LR (p<0.05). Conversion from LR to OR was required by 11.3% of the patients (13/115). The intraoperative complication rate was 30.8% for the patients who underwent conversion. The operation time and postoperative complication rate were the same as for LR alone (p>0.05). The local recurrence rate was 3.7% for the LR group and 4.9% for the OR group (chi (2)=0.209; p=0.647) during the 20-month median follow-up period. CONCLUSIONS: The findings showed that LR for rectal cancer was safe and effective, resulting in faster recovery and a similar complication rate compared with OR. Conversion did not alter the patients' outcomes.


Assuntos
Colectomia/métodos , Laparoscopia/métodos , Neoplasias Retais/cirurgia , China/epidemiologia , Feminino , Seguimentos , Humanos , Masculino , Pessoa de Meia-Idade , Morbidade/tendências , Recidiva Local de Neoplasia/epidemiologia , Estudos Retrospectivos , Taxa de Sobrevida/tendências , Resultado do Tratamento
10.
World J Surg Oncol ; 7: 31, 2009 Mar 18.
Artigo em Inglês | MEDLINE | ID: mdl-19296838

RESUMO

BACKGROUND: Hemolymphangioma of the pancreas is a very rare benign tumor. There were only six reports of this disease until December 2008. Herein, we report a case of giant hemolymphangioma of the pancreas in a 20-year-old girl. CASE PRESENTATION: We describe a 20-year-old girl who presented with a mass in abdominal cavity and epigastric discomfort about a week. Physical examination showed a great abdominal mass. Abdominal computed tomography showed extrinsic duodenal compression due to a large retroperitoneal tumor possibly arising from pancreas. The tumor enucleation was performed and a diagnosis of hemolymphangioma of the pancreas was made. The patient had a complication of chylous leakage, which was successfully managed. The patient is alive and well, after 26 months of follow-up, with no complaints or recurrence. CONCLUSION: From this case and literature, we can conclude that hemolymphangioma of the pancreas in adult is a rare benign tumor, and accurate diagnosis can not be preoperatively established. Tumor resection should be performed whenever possible. The risk of recurrence seems very low.


Assuntos
Linfangioma/patologia , Neoplasias Pancreáticas/patologia , Adulto , Feminino , Humanos , Linfangioma/diagnóstico , Linfangioma/cirurgia , Neoplasias Pancreáticas/diagnóstico , Neoplasias Pancreáticas/cirurgia
11.
Inflammation ; 39(5): 1835-41, 2016 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-27525425

RESUMO

In this study, we investigated the effects of astragaloside IV (As-IV) on pulmonary fibrosis and its mechanisms of action. Sprague-Dawley rats were used in a model of pulmonary fibrosis induced by an intratracheal instillation of bleomycin (BLM). Rats were intraperitoneally injected with As-IV (10, 20, 50 mg/kg) daily for 28 days, while the rats in control and BLM groups were injected with a saline solution. The effects of As-IV treatment on pulmonary injury were evaluated with the lung wet/dry weight ratios, cell counts, and histopathologic. Oxidative stress was evaluated by detecting the levels of malondialdehyde (MDA), superoxide dismutase (SOD), total antioxidant capacity (T-AOC), and reactive oxygen species (ROS) in lung tissue. Inflammation was assessed by measuring the levels of tumor necrosis factor (TNF)-α, interleukin (IL)-1ß, and IL-6 in bronchoalveolar lavage fluid (BALF). The results indicated that As-IV treatment remarkably ameliorated BLM-induced pulmonary fibrosis and attenuated BLM-induced oxidative stress and inflammation. Our findings indicate that As-IV-mediated suppression of fibroproliferation may contribute to the anti-fibrotic effect against BLM-induced pulmonary fibrosis. Its mechanisms of action are associated with inhibiting oxidative stress and inflammatory response. In summary, our study suggests a therapeutic potential of As-IV in the treatment of pulmonary fibrosis.


Assuntos
Saponinas/farmacologia , Triterpenos/farmacologia , Animais , Bleomicina , Relação Dose-Resposta a Droga , Inflamação/diagnóstico , Inflamação/tratamento farmacológico , Estresse Oxidativo/efeitos dos fármacos , Fibrose Pulmonar/induzido quimicamente , Fibrose Pulmonar/diagnóstico , Fibrose Pulmonar/tratamento farmacológico , Ratos , Ratos Sprague-Dawley , Saponinas/administração & dosagem , Triterpenos/administração & dosagem
12.
J Endocrinol ; 230(1): 55-65, 2016 07.
Artigo em Inglês | MEDLINE | ID: mdl-27149986

RESUMO

Chemerin, a chemokine, plays important roles in immune responses, inflammation, adipogenesis, and carbohydrate metabolism. Our recent research has shown that chemerin has an inhibitory effect on hormone secretion from the testis and ovary. However, whether G protein-coupled receptor 1 (GPR1), the active receptor for chemerin, regulates steroidogenesis and luteolysis in the corpus luteum is still unknown. In this study, we established a pregnant mare serum gonadotropin-human chorionic gonadotropin (PMSG-hCG) superovulation model, a prostaglandin F2α (PGF2α) luteolysis model, and follicle and corpus luteum culture models to analyze the role of chemerin signaling through GPR1 in the synthesis and secretion of gonadal hormones during follicular/luteal development and luteolysis. Our results, for the first time, show that chemerin and GPR1 are both differentially expressed in the ovary over the course of the estrous cycle, with highest levels in estrus and metestrus. GPR1 has been localized to granulosa cells, cumulus cells, and the corpus luteum by immunohistochemistry (IHC). In vitro, we found that chemerin suppresses hCG-induced progesterone production in cultured follicle and corpus luteum and that this effect is attenuated significantly by anti-GPR1 MAB treatment. Furthermore, when the phosphoinositide 3-kinase (PI3K) pathway was blocked, the attenuating effect of GPR1 MAB was abrogated. Interestingly, PGF2α induces luteolysis through activation of caspase-3, leading to a reduction in progesterone secretion. Treatment with GPR1 MAB blocked the PGF2α effect on caspase-3 expression and progesterone secretion. This study indicates that chemerin/GPR1 signaling directly or indirectly regulates progesterone synthesis and secretion during the processes of follicular development, corpus luteum formation, and PGF2α-induced luteolysis.


Assuntos
Quimiocinas/metabolismo , Corpo Lúteo/metabolismo , Peptídeos e Proteínas de Sinalização Intercelular/metabolismo , Luteólise/fisiologia , Receptores Acoplados a Proteínas G/metabolismo , Transdução de Sinais/fisiologia , Superovulação/fisiologia , Animais , Caspase 3/metabolismo , Gonadotropina Coriônica/farmacologia , Corpo Lúteo/efeitos dos fármacos , Dinoprosta/farmacologia , Estradiol/metabolismo , Feminino , Luteólise/efeitos dos fármacos , Camundongos , Fosfatidilinositol 3-Quinases/metabolismo , Progesterona/metabolismo , Transdução de Sinais/efeitos dos fármacos
13.
World J Gastroenterol ; 11(36): 5651-4, 2005 Sep 28.
Artigo em Inglês | MEDLINE | ID: mdl-16237759

RESUMO

AIM: To study the effect of SNC19/ST14 gene overexpression on invasion in vitro of colorectal cancer cells. METHODS: The adhesion of SNC19/ST14 gene-transfected cells to ECM was measured by MTT assay. The cell movement was evaluated by wound healing assay. Cell invasion and migration were determined by invasion assay in vitro. RESULTS: SNC19/ST14 gene overexpression could enhance invasion of colorectal cancer cells in vitro significantly and influence early cell adherence to ECM, but could not change cell movement significantly. CONCLUSION: SNC19/ST14 gene overexpression increases the local invasion of colorectal cancer cells in vitro.


Assuntos
Neoplasias Colorretais/genética , Neoplasias Colorretais/patologia , Invasividade Neoplásica , Serina Endopeptidases/genética , Serina Endopeptidases/metabolismo , Adesão Celular , Linhagem Celular Tumoral , Movimento Celular , Regulação Neoplásica da Expressão Gênica , Humanos
14.
Parasitol Int ; 54(4): 231-6, 2005 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-16231860

RESUMO

To assess the epidemiological efficacy of type SRP III slow-released praziquantel-medicated bars for dogs in the prevention and control of cystic echinococcosis in man and livestock, praziquantel-medicated bars were implanted subcutaneously in over 90% of dogs in villages in north Xinjiang, China, where cystic echinococcosis is highly endemic. After implantation, infection rate of Echinococcus granulosus in dogs, specific antibodies in children and prevalence of echinococcosis in one-year-old lambs were observed for 3 years. Coproantigen of E. granulosus was positive in 41.2% of the dogs at the start of experiment. In the second and third year after medicated-bar implantation, coproantigen was undetectable in any dogs examined, while 3.0% of dogs were positive at the end of the fourth year. The antibody positive rate in 7-year-old pupils, that was 41.2% before the experiment, declined gradually and it was 5.4% in the fourth year, while children in the non-intervention control area showed 30.6% seropositivity. The prevalence of hydatid disease in children aged 7-16 years also declined significantly. The prevalence of hydatidosis in lambs one year of age was 44.8% in the first year, dropping to 10.7% in the fourth year, while in the non-intervention control area the level of infection was 46.4%. These results demonstrated not only that the slow released praziquantel-medicated bars efficiently blocked reinfection in dogs at least for 2 years, but also the measure was effective in preventing transmission of cystic echinococcosis to both man and livestock.


Assuntos
Anti-Helmínticos/uso terapêutico , Preparações de Ação Retardada/uso terapêutico , Doenças do Cão/epidemiologia , Implantes de Medicamento/uso terapêutico , Equinococose/epidemiologia , Echinococcus granulosus/efeitos dos fármacos , Praziquantel/uso terapêutico , Doenças dos Ovinos/epidemiologia , Adolescente , Animais , Anti-Helmínticos/administração & dosagem , Anticorpos Anti-Helmínticos/sangue , Criança , China , Preparações de Ação Retardada/administração & dosagem , Doenças do Cão/tratamento farmacológico , Doenças do Cão/parasitologia , Doenças do Cão/prevenção & controle , Cães , Implantes de Medicamento/administração & dosagem , Equinococose/tratamento farmacológico , Equinococose/parasitologia , Equinococose/prevenção & controle , Echinococcus granulosus/imunologia , Echinococcus granulosus/isolamento & purificação , Humanos , Praziquantel/administração & dosagem , Prevalência , Saúde da População Rural , Ovinos , Doenças dos Ovinos/parasitologia
15.
Zhejiang Da Xue Xue Bao Yi Xue Ban ; 34(1): 38-42, 2005 01.
Artigo em Chinês | MEDLINE | ID: mdl-15693122

RESUMO

OBJECTIVE: To investigate the relationship between SNC19 protein and cancer metastasis. METHODS: Expression of SNC19 protein in cancer cell lines and tissues was examined by Western blot analysis using anti-SNC19 monoclonal antibody. In addition, Psectag2A-SNC19(ORF) eukaryotic expression vector was constructed and transfected into BCAP37 cells. After the target protein was expressed and purified, processing forms of SNC19 protein were further identified using anti-His mAb and each form was assayed for its gelatinase activity. RESULTS: Different expression and post-translational processing of the SNC19 proteins in the cancer cell lines and intestinal tissues were detected.BCAP37 cells transfected full-length SNC19 (ORF) generated two different sized proteins in cell lysates, 120 and 75 kD; 75 kD was detected to have proteolytic activity by gelatin zymography. CONCLUSION: SNC19 protein presents different expression and post-translational processing in the cancer cells and tissues, of which 75 kD was identified to have gelatinase activity.


Assuntos
Neoplasias da Mama/genética , Proteínas de Neoplasias/metabolismo , Processamento de Proteína Pós-Traducional , Serina Endopeptidases/metabolismo , Neoplasias da Mama/metabolismo , Neoplasias da Mama/patologia , Linhagem Celular Tumoral , Neoplasias do Colo/genética , Neoplasias do Colo/metabolismo , Neoplasias do Colo/patologia , DNA Complementar/química , Feminino , Gelatinases/metabolismo , Humanos , Metástase Neoplásica , Proteínas de Neoplasias/química , Proteínas de Neoplasias/genética , Proibitinas , Serina Endopeptidases/química , Serina Endopeptidases/genética , Transfecção
16.
Zhonghua Yi Xue Za Zhi ; 84(10): 843-8, 2004 May 17.
Artigo em Chinês | MEDLINE | ID: mdl-15200890

RESUMO

OBJECTIVE: To study the effect of SNC19/ST14 gene transfection and expression into colorectal cancer cells on biological behavior. METHODS: The recombination vector pSecTag2a-SNC19/ST14 was constructed, and transfected into the RKO colorectal cancer cell line by liposome. The transfected cell was screened by real time PCR, Western Blot and Immunohistochemical technique. The population doubling time (T(D)) and cell cycle of the transfected cell were analyzed by MTT assay and flow cytometer. Rhodamine-labeled Phallodin was used to label the cell cytoskeletal protein-F-actin, and the F-actin distribution was observed by confocal scanning microscope. The adhesion ability of the transfected cell to extracellular matrix (ECM) was measured by MTT assay. RESULTS: The full length Open Reading Frame (ORF) of SNC19/ST14 gene was inserted into the vector pSecTag2a, and transfected into RKO cells, and expressed successfully. The changes of F-actin organization took place in transfected cell. The Adherence ability of the transfected cell to ECM was decreased, but the proliferation ability was not significantly changed except highly expressing of the SNC19/ST14, and not the cell cycle and the apoptosis. CONCLUSION: SNC19/ST14 gene was successfully expressed into the RKO cell line, and could influence on the cell cytoskeletal protein (F-actin) organization and on cell adherence ability to ECM, but could not make the cell cycle, apoptosis and proliferation ability change significantly.


Assuntos
Actinas/genética , Neoplasias Colorretais/genética , Serina Endopeptidases/genética , Actinas/metabolismo , Western Blotting , Linhagem Celular Tumoral , Citometria de Fluxo , Expressão Gênica , Regulação da Expressão Gênica , Humanos , Imuno-Histoquímica , Reação em Cadeia da Polimerase , Serina Endopeptidases/fisiologia
17.
Zhonghua Yi Xue Za Zhi ; 83(23): 2077-82, 2003 Dec 10.
Artigo em Chinês | MEDLINE | ID: mdl-14703421

RESUMO

OBJECTIVE: To explore the transcription regulation of DNA 5'CpG island demethylation on p16/CDKN2 tumor suppressor gene and effects of growth on RKO human colorectal cancer cell line. METHODS: RKO cell line was exposed to the specific demethylating agent, 5-Aza-2'-deoxycytidine, for seventy-two hours to detect whether the silencing of p16/CDKN2 cell cycle regulatory gene could be reversed. Methylation-specific PCR (MSP), T-A cloning and sequence analysis were evaluated for methylation status. Growth speed, expression of DNA methyltransferase mRNA, p16/CDKN2 mRNA and protein were determined by MTT assay, reverse transcription polymerase chain reaction (RT-PCR), Real-time PCR, immunohistochemistry and western blot. RESULTS: (1) All cytosines in CpG dinucleotides in untreated RKO cells with 5-Aza-2'-deoxycytidine remain as C, while all cytosines in treated RKO cells have been converted to thymidine. (2) RKO cell line after treatment with three different concentration 5-Aza-2'-deoxycytidine grew slowly and double time increased to 1.49, 1.64, 1.87-times respectively. (3) The expression of RKO cell p16/CDNK2 gene mRNA treated with 5-Aza-2'-deoxycytidine increased to 4.89, 16.91, 19.97-times respectively, but the expression of DNA methyltransferase mRNA was inhibited. (4) Immunohistochemistry and western blot indicated that 5-Aza-2'-deoxycytidine could increase the p16/CDKN2 gene protein expression. CONCLUSION: DNA promoter hypermethylation is associated with p16/CDKN2 gene silence in RKO human colorectal cancer cell line. 5-Aza-2'-deoxycytidine may effectively cause demethylation and inhibit the growth of tumor cell by reactivating the gene transcription silenced by aberrant hypermethylation.


Assuntos
Antimetabólitos Antineoplásicos/farmacologia , Azacitidina/análogos & derivados , Azacitidina/farmacologia , Neoplasias Colorretais/genética , Metilação de DNA , Metilases de Modificação do DNA/antagonistas & inibidores , Genes p16 , Transcrição Gênica/efeitos dos fármacos , Sequência de Bases , Western Blotting , Linhagem Celular Tumoral , Neoplasias Colorretais/patologia , Decitabina , Humanos , Imuno-Histoquímica , Dados de Sequência Molecular , RNA Mensageiro/análise
18.
World J Biol Chem ; 5(3): 301-7, 2014 Aug 26.
Artigo em Inglês | MEDLINE | ID: mdl-25225598

RESUMO

Colorectal cancer is the third most common cancer worldwide. Metastasis is a major cause of colorectal cancer-related death. Mechanisms of metastasis remain largely obscure. MicroRNA is one of the most important epigenetic regulators by targeting mRNAs post-transcriptionally. Accumulated evidence has supported its significant role in the metastasis of colorectal cancer, including epithelial-mesenchymal transition and angiogenesis. Dissecting microRNAome potentially identifies specific microRNAs as biomarkers of colorectal cancer metastasis. Better understanding of the complex network of microRNAs in colorectal cancer metastasis provide new insights in the biological process of metastasis and in the potential targets for colorectal cancer therapies and for diagnosis of recurrent and metastatic colorectal cancer.

19.
Oncol Lett ; 8(4): 1455-1460, 2014 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-25202349

RESUMO

Gastrointestinal stromal tumors (GISTs) are rare in the rectum. Radical surgery, such as an abdominoperineal resection, is necessary for large rectal GISTs, which can result in the loss of function of involved organs. Imatinib mesylate can be used as perioperative therapy and may reduce tumor size, and it is now approved for use in the adjuvant therapy of locally resected anorectal GISTs. The present study describes two cases of large rectal GISTs, for which abdominoperineal resections were initially planned. The two patients received pre-operative imatinib mesylate treatment, and the therapeutic response was assessed by magnetic resonance imaging. Finally, transsacral local resection was successfully performed for these two GISTs. A macroscopically complete resection was achieved, and microscopically, the resection margin was negative. One patient experienced the complication of rectal leakage, which was successfully managed by drainage. No recurrence occurred in the two patients after more than two years. Pre-operative imatinib mesylate therapy with subsequent transsacral local resection for selected rectal GISTs is a feasible treatment modality and can prevent extended surgery.

20.
Asian Pac J Trop Med ; 6(5): 404-6, 2013 May 13.
Artigo em Inglês | MEDLINE | ID: mdl-23608382

RESUMO

OBJECTIVE: To discuss the expression and significance of vascular endothelial growth factor (VEGF) and p53 expression in rat degenerated intervertebral disc (IVD) tissues. METHODS: A total of 78 even-aged Sprague-Dawley (SD) rats (observation group) were chosen to establish a rat IVD degeneration model with anterior limbs and tail-removing method. Three months after modeling, rats were executed and degenerated IVD tissues were obtained for pathological biopsy so as to observe VEGF and p53 expression thereof. Meanwhile, IVD tissues from 8 healthy rats were gained for comparison. RESULTS: Capillary infiltration was found in 62.8% of cases in observation group. Positive expression of VEGF and p53 was seen in 74.4% and 59.0% of cases respectively with a co-expression rate of 53.8%. VEGF and p53 expression in degenerated IVD tissues with capillary infiltration was higher than that in non-infiltration group (P<0.05). CONCLUSIONS: A coordinate expression of VEGF and p53 in rat degenerated IVD tissues is demonstrated, indicating that they are both involved in neovascularization and infiltration, which accelerates IVD degeneration.


Assuntos
Degeneração do Disco Intervertebral/metabolismo , Disco Intervertebral/metabolismo , Proteína Supressora de Tumor p53/metabolismo , Fator A de Crescimento do Endotélio Vascular/metabolismo , Animais , Distribuição de Qui-Quadrado , Imuno-Histoquímica , Disco Intervertebral/patologia , Degeneração do Disco Intervertebral/patologia , Masculino , Ratos , Ratos Sprague-Dawley
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