RESUMO
Developmental neurology is one of the major areas of neuropediatrics and is among other things (legally) responsible for monitoring the motor, cognitive and psychosocial development of all infants using standardized monitoring investigations. The special focus is on infants born at risk and/or due to premature birth before 32 weeks of gestation or a birth weight less than 1500 g. Early diagnosis of deviations from normal, age-related development is a prerequisite for early interventions, which may positively influence development and the long-term biopsychosocial prognosis of the patients. This article illustrates the available methods in developmental neurology with a focus on recent developments. Particular attention is paid to the predictive value of general movements (GM). The current development of markerless automated detection of spontaneous movements using conventional depth imaging cameras is demonstrated. Differences in spontaneous movements in infants at the age of 12 weeks are illustrated and discussed exemplified by three patients (healthy versus genetic syndrome versus cerebral palsy).
Assuntos
Paralisia Cerebral/diagnóstico , Paralisia Cerebral/terapia , Doenças do Prematuro/diagnóstico , Doenças do Prematuro/terapia , Comunicação Interdisciplinar , Colaboração Intersetorial , Exame Neurológico , Diagnóstico Precoce , Intervenção Médica Precoce , Humanos , Recém-Nascido de muito Baixo Peso , Atividade MotoraRESUMO
INTRODUCTION: External brain irradiation in children can cause cognitive decline, endocrine dysfunctions and second malignancies. A rare complication is cerebral vasculopathy, which occurs most often in patients with neurofibromatosis type 1. Interstitial radiotherapy using transient Iodine-125 implants is a radiotherapy option, called brachytherapy, offering excellent survival rates, but little is known on treatment-related morbidity, especially long time vascular changes. PATIENTS AND METHODS: Thirteen children with low-grade hypothalamic gliomas, four of them with neurofibromatosis type 1, were diagnosed and treated at the University Hospital Freiburg, Germany. They belong to a larger group of 44 children with suprasellar low-grade gliomas, treated with transient Iodine-125 seeds and include those who attended all routine follow-up examinations in Freiburg. After written informed consent from the parents or caregivers all patients underwent magnetic resonance imaging with angiographic techniques in 2001, 3 to 13 years after treatment. RESULTS AND DISCUSSION: Six out of 13 revealed cerebral vasculopathies, only one of them revealed symptoms of intermittent cerebral ischemia. Neurofibromatosis type 1 was present in one affected patient. The aetiology of the cerebral vascular changes is not fully understood so far. Tumour encasement, surgical damage and brachytherapy may contribute as a single risk factor or in combination. To get more information, we recommend MRA for artery vasculopathy at follow-up in all patients with suprasellar brain tumours irrespectively to their former treatment or presence of cerebrovascular symptoms.
Assuntos
Braquiterapia/efeitos adversos , Transtornos Cerebrovasculares/epidemiologia , Glioma/radioterapia , Neoplasias Hipotalâmicas/radioterapia , Quiasma Óptico/patologia , Lesões por Radiação/epidemiologia , Neoplasias Encefálicas/epidemiologia , Neoplasias Encefálicas/radioterapia , Transtornos Cerebrovasculares/etiologia , Criança , Pré-Escolar , Feminino , Seguimentos , Glioma/epidemiologia , Humanos , Neoplasias Hipotalâmicas/epidemiologia , Incidência , Lactente , Masculino , Quiasma Óptico/efeitos da radiação , Lesões por Radiação/etiologia , Estudos Retrospectivos , Fatores de RiscoRESUMO
BACKGROUND: Aicardi-Goutières syndrome (AGS) is an autosomal recessive, early onset encephalopathy characterised by calcification of the basal ganglia, chronic cerebrospinal fluid lymphocytosis, and negative serological investigations for common prenatal infections. AGS may result from a perturbation of interferon alpha metabolism. The disorder is genetically heterogeneous with approximately 50% of families mapping to the first known locus at 3p21 (AGS1). METHODS: A genome-wide scan was performed in 10 families with a clinical diagnosis of AGS in whom linkage to AGS1 had been excluded. Higher density genotyping in regions of interest was also undertaken using the 10 mapping pedigrees and seven additional AGS families. RESULTS: Our results demonstrate significant linkage to a second AGS locus (AGS2) at chromosome 13q14-21 with a maximum multipoint heterogeneity logarithm of the odds (LOD) score of 5.75 at D13S768. The AGS2 locus lies within a 4.7 cM region as defined by a 1 LOD-unit support interval. CONCLUSIONS: We have identified a second AGS disease locus and at least one further locus. As in a number of other conditions, genetic heterogeneity represents a significant obstacle to gene identification in AGS. The localisation of AGS2 represents an important step in this process.
Assuntos
Doenças dos Gânglios da Base/genética , Calcinose/genética , Cromossomos Humanos Par 13 , Linfocitose/genética , Doenças dos Gânglios da Base/diagnóstico , Calcinose/diagnóstico , Mapeamento Cromossômico , Estudos de Coortes , Consanguinidade , Feminino , Genes Recessivos , Ligação Genética , Genótipo , Humanos , Lactente , Recém-Nascido , Linfocitose/diagnóstico , Masculino , SíndromeRESUMO
This study was done to investigate the possible role of histaminergic systems in electroshock seizures. Brain histamine concentrations in rats were elevated by metoprine (i.p.), an inhibitor of histamine-N-methyltransferase. Animals were tested for their response to maximal electroshock (MES) at different times after the injection. Metoprine raised brain histamine concentrations and inhibited maximal hindleg extension after MES in a dose-dependent manner. Sensitivity to seizures correlated inversely with histamine concentrations. These results suggest that histaminergic neurones are involved in mechanisms which inhibit generalizations of epileptic discharges in the brain.
Assuntos
Histamina/fisiologia , Pirimetamina/análogos & derivados , Convulsões/fisiopatologia , Animais , Eletrochoque , Feminino , Histamina N-Metiltransferase/antagonistas & inibidores , Masculino , Inibição Neural , Ratos , Ratos Endogâmicos , Convulsões/etiologia , Transmissão SinápticaRESUMO
The convulsive thresholds for bicuculline and electroshock seizures were studied in audiogenic seizure (AGS)-susceptible and control rats. Electroshock seizure thresholds, determined as the amperage necessary to cause tonic extension of the hindlegs in 50% of the rats (CC50 = convulsive current fifty) were markedly lowered in rats of two stocks, bred for AGS susceptibility. During the clonic phase of electroshock seizure the bicuculline threshold was slightly lowered but started to rise after the convulsion had ceased. After 5 min, the threshold was significantly elevated and the maximal increase was reached in 15 min. In control rats the level normalized curvilinearly within an hour, but in AGS rats it decreased more slowly and was still elevated after 90 min. After an audiogenic seizure, the threshold for bicuculline-induced seizures in AGS rats also rose significantly but declined rapidly after having reached a maximum at 15 min. This rise in seizure threshold for bicuculline might indicate a postictal change in GABAergic transmission.
Assuntos
Convulsões/fisiopatologia , Estimulação Acústica , Animais , Bicuculina/farmacologia , Eletrochoque , Feminino , Masculino , Ratos , Ratos Endogâmicos , Fatores de TempoRESUMO
Intravenous administration of 1-methyl-4-phenyl-1,2,3,6,-tetrahydropyridine (MPTP) leads to the progressive development of a model of Parkinson's disease in the primate. The development of damage occurring in the striatal area during MPTP-treatment was followed "in vivo" in a baboon by positron emission tomography (PET). Spiperone labelled with a positron emitter 76Br (76Br-BSP) was used for the quantitative "in vivo" imaging of D2 dopamine receptors. The decrease in the striatal binding of 76Br-BSP measured "in vivo", after three series of MPTP injections, paralleled the increase in the severity of behavioral symptoms seen immediately after administration of the neurotoxin. At the end of the MPTP-treatment when neurological symptoms were the most important, a 36% decrease in the 76Br-BSP specific binding was measured. Between the series of MPTP injections a partial recovery in the quantitative measurement of the 76Br-BSP specific binding occurring in the striatum was well correlated with the disappearance of the neurological syndrome. Post-mortem histological and biochemical studies in nigro-striatal anatomical structures of MPTP-intoxicated primates compared with control animals showed a 80% loss of neuronal cell bodies in the substantia nigra compacta and a 42% decrease in the density (Bmax) of D2 receptors (in vitro 3H-spiperone binding). All these results showed that the use of PET and 76Br-BSP allow to follow in a noninvasive way both the degenerative processes and the subsequent partial recovery which occur in dopaminergic striatal receptor function during MPTP-treatment.
Assuntos
Corpo Estriado/diagnóstico por imagem , Piridinas/farmacologia , Receptores Dopaminérgicos/efeitos dos fármacos , 1-Metil-4-Fenil-1,2,3,6-Tetra-Hidropiridina , Animais , Bromo , Corpo Estriado/efeitos dos fármacos , Modelos Animais de Doenças , Masculino , Papio , Doença de Parkinson Secundária/diagnóstico por imagem , Radiografia , Radioisótopos , Espiperona , Tomografia Computadorizada de EmissãoRESUMO
Twenty rats of an audiogenic seizure (AGS)-susceptible stock were exposed to four different sound stimuli and seizure severity and seizure-latency were registered. Two sounds with harmonic spectra, one pure tone and band noise were used. The harmonic spectra were found to be significantly (P less than 0.005) more effective than the other stimuli in inducing AGS when seizure-latency was taken as a parameter. Connections from the auditory pathway to the reticular formation seem to be of importance in the triggering of these seizures. AGS-susceptible rats may offer a useful tool for experimental hearing research.
Assuntos
Estimulação Acústica , Convulsões/etiologia , Som , Animais , Vias Auditivas/fisiologia , Feminino , Masculino , RatosRESUMO
In order to further elucidate the possible role of histamine in the seizure model, we determined the histamine levels in different brain regions of genetically epilepsy-prone Krushinski-Molodkina (KM) rats. Histamine levels in the striatum, hippocampus, amygdala, midbrain, thalamus and hypothalamus of KM rats were significantly lower than in the epilepsy-resistant Wistar rats. Previously, we have reported that the audiogenic seizures of KM rats were reduced by metoprine, which can markedly increase brain histamine. These findings are in agreement with the hypothesis that central histamine neuron system may be involved in the inhibitory mechanism of seizures.
Assuntos
Química Encefálica , Epilepsia/metabolismo , Histamina/análise , Estimulação Acústica , Animais , Feminino , Masculino , Ratos , Ratos EndogâmicosRESUMO
A review of the literature on sleeping "disorders" in infants and toddlers shows that sleep problems are very common. We report the results of a German multicenter epidemiological study on a birth cohort (N = 1314). The children had medical examinations at 4 weeks, 3 months, 6 months, 1 year, 18 months, 2 years and 3 years. Most of the information on the sleeping behavior was gathered by structured interview and questionnaires. This paper gives information on the prevalence and the persistence of sleep problems in one to three year old children in Germany. Statistical analysis of correlations between breastfeeding and sleep and the place of sleep are reported. The consequences of sleep problems on the family overall well-being are also examined.
Assuntos
Fases do Sono , Transtornos do Sono-Vigília/epidemiologia , Pré-Escolar , Ritmo Circadiano , Estudos de Coortes , Estudos Transversais , Feminino , Seguimentos , Alemanha/epidemiologia , Humanos , Incidência , Lactente , Recém-Nascido , Masculino , Transtornos do Sono-Vigília/etiologiaAssuntos
Benzodiazepinas/administração & dosagem , Benzodiazepinas/efeitos adversos , Síndrome de Abstinência a Substâncias/diagnóstico , Ansiedade/tratamento farmacológico , Doença Crônica , Esquema de Medicação , Feminino , Humanos , Masculino , Prognóstico , Medição de Risco , Síndrome de Abstinência a Substâncias/terapiaAssuntos
Encéfalo/efeitos dos fármacos , Drogas Ilícitas , Síndrome de Abstinência a Substâncias/tratamento farmacológico , Transtornos Relacionados ao Uso de Substâncias/tratamento farmacológico , Benzodiazepinas/efeitos adversos , Benzodiazepinas/farmacologia , Encéfalo/metabolismo , Cannabis/efeitos adversos , Estimulantes do Sistema Nervoso Central/efeitos adversos , Estimulantes do Sistema Nervoso Central/farmacologia , Atenção à Saúde/organização & administração , Dopamina/metabolismo , Política de Saúde/legislação & jurisprudência , Humanos , Entorpecentes/efeitos adversos , Entorpecentes/farmacologia , Nicotina/efeitos adversos , Nicotina/farmacologia , Núcleo Accumbens/efeitos dos fármacos , Núcleo Accumbens/metabolismo , Abuso de Fenciclidina/diagnóstico , Abuso de Fenciclidina/tratamento farmacológico , Abuso de Fenciclidina/etiologia , Abuso de Fenciclidina/psicologia , Síndrome de Abstinência a Substâncias/diagnóstico , Síndrome de Abstinência a Substâncias/etiologia , Síndrome de Abstinência a Substâncias/psicologia , Transtornos Relacionados ao Uso de Substâncias/diagnóstico , Transtornos Relacionados ao Uso de Substâncias/etiologia , Transtornos Relacionados ao Uso de Substâncias/psicologiaRESUMO
Canavan disease is characterised as a rare, neurodegenerative disease that usually causes death in early childhood. It is an autosomal recessive disorder due to an aspartoacylase (ASPA) deficiency. The causative gene has been mapped to chromosome 17 pter-p13. Here we describe three affected children from two Greek families with an unusually mild course of Canavan disease. All children presented with muscular hypotonia and macrocephaly. Diagnosis was based on elevated N-acetylaspartate in urine, reduced aspartoacylase activity in fibroblasts, and marked white matter changes on cerebral imaging. All three affected individuals exhibited continuous psychomotor development without any regression. Genetic analyses revealed compound heterozygous mutations (Y288 C; F295 S) in two individuals. The Y288 C variant was previously described in a child with macrocephaly, mild developmental delay, increased signal intensity in the basal ganglia, partial cortical blindness and retinitis pigmentosa, and slightly elevated N-acetylaspartate in the urine. Demonstration of the same variant in two unusually mildly affected Canavan disease patients and absence of this variant in 154 control chromosomes suggest a possible pathogenic role in mild Canavan disease. In the third individual, two homozygous sequence variants were identified, which comprise the known G274R mutation and a novel K213E variant.
Assuntos
Amidoidrolases/genética , Doença de Canavan/genética , Mutação , Fenótipo , Adolescente , Amidoidrolases/deficiência , Doença de Canavan/patologia , Doença de Canavan/fisiopatologia , Criança , Pré-Escolar , Análise Mutacional de DNA/métodos , Feminino , Genótipo , Humanos , Imageamento por Ressonância Magnética/métodos , MasculinoRESUMO
Clonidine in high doses (0.5,1.0 mg/kg) significantly increased the latency for audiogenic seizures (AGS) in rats and reduced seizure severity. At a dose (0.05 mg/kg) which acts more specifically on presynaptic alpha 2-receptors, clonidine did not affect seizure latency, but showed a slight proconvulsant action. Yohimbine tended to decrease seizure-latency at all doses, but statistical significance (p less than 0.05) was only reached at 10 mg/kg. Smaller doses of yohimbine (0.5 and 1.0 mg/kg) showed a proconvulsant effect, while a high dose (10 mg/kg) markedly reduced the severity of AGS. The effect of clonidine on seizure-latency was only antagonized by high-dose yohimbine (10 mg/kg), the combination of these drugs being of marked anticonvulsant efficacy. From these results it can be concluded that the anticonvulsant effect of clonidine does not seem to be mediated through presynaptic alpha 2-receptors. Action on other central noradrenergic receptors, and influences on other transmitters must be taken into account when interpreting the effect of clonidine and yohimbine on AGS in rats.
Assuntos
Anticonvulsivantes , Clonidina/farmacologia , Convulsões/prevenção & controle , Ioimbina/farmacologia , Estimulação Acústica , Animais , Norepinefrina/farmacologia , RatosRESUMO
Benzodiazepine receptors were investigated in the cerebral cortex, the hippocampus, the brainstem, and the cerebellum of audiogenic seizure (AGS)-susceptible and seizure-resistant (ER) control rats. In AGS-susceptible rats of Sprague-Dawley descent, muscimol (10-6 M and 3 x 10-5 M) activated the binding of 3H-diazepam (0.4 nM) significantly less than in ER-rats. This finding may be strain selective, since it was not observed in AGS-susceptible rats of Wistar descent. Specific binding of the convulsant benzodiazepine receptor ligand methyl 6,7-dimethoxy-4-ethyl carboline-3-carboxylate (3H-DMCM), the benzodiazepine receptor ligand 3H-diazepam and the chloride channel directed cage convulsant t-butylbicyclophosphorothionate 35S-TBPS were not significantly changed in AGS-susceptible as compared to control rats. Our findings indicate that a disturbance at the level of the benzodiazepine receptor/GABA receptor/chloride channel complex is not a likely general aetiological factor for audigenic seizures in rats.
Assuntos
Compostos Bicíclicos Heterocíclicos com Pontes , Receptores de GABA-A/metabolismo , Convulsões/metabolismo , Estimulação Acústica , Animais , Compostos Bicíclicos com Pontes/metabolismo , Carbolinas/metabolismo , Convulsivantes/metabolismo , Diazepam/metabolismo , Feminino , Flunitrazepam/metabolismo , Técnicas In Vitro , Masculino , Muscimol/farmacologia , Ratos , Ratos Endogâmicos , Receptores de GABA-A/efeitos dos fármacosRESUMO
An apparatus for pure tone stimulation of audiogenic seizure (AGS)-susceptible rats is described, and the effect of change in sound pressure level and frequency on AGS response in Uaz:AGS (SD) rats is shown. By use of pure tone stimulation, the prevalence of AGS response in rats of this strain, which have been bred for seizure-susceptibility, is 77%. With increasing frequency, the intensity-response curves show a shift to lower sound pressure levels, the ED50 for 20 kHz (83.8 dB) being significantly lower than the ED50 for 5 (110.5 dB) and 10 kHz (97.5 dB). Repeated exposure to seizure-inducing sound at weekly intervals leads to more severe seizures in individual rats. This should be taken into consideration as one possible confounding factor when using this method for assaying the efficacy of anticonvulsant drugs.