Biokinetics of 13C in the human body after oral administration of 13C-labeled glucose as an index for the biokinetics of 14C.

The retention of 13C in the human body after oral administration of 13C-labeled glucose was studied in three healthy volunteer subjects to estimate the 50 year cumulative body burden for 13C as an index of the committed dose of the radioisotope 14C. After administration of 13C-labeled glucose, the volunteers ingested controlled diets with a fixed number of calories for 112 d. Samples of breath and urine were collected up to 112 d after administration. Samples of feces were collected up to 14 d after administration. Hair samples were obtained at 119 d after administration and analyzed as a representative index of the rate of excretion of organic 13C via pathways such as skin cell exfoliation and mucus secretion. All samples were analyzed for 13C/12C atomic ratio to determine the rate of excretion via each pathway. We then constructed a metabolic model with a total of four pathways (breath, urine, feces, and other) comprising seven compartments. We determined the values of the biokinetic parameters in the model by using the obtained excretion data. From 74% to 94% of the 13C administered was excreted in breath, whereas <2% was excreted in urine and feces. In the other pathway, the excretion rate constant in the compartment with the longest residence time stretched to hundreds of days but the rate constant for each subject was not statistically significant (P value > 0.1). In addition, the dataset for one of the three subjects was markedly different from those of the other two. When we estimated the 50 year cumulative body burden for 13C by using our model and we included non-statistically significant parameters, a considerable cumulative body burden was found in the compartments excreting to the other pathway. Although our results on the cumulative body burden of 13C from orally administered carbon as glucose were inconclusive, we found that the compartments excreting to the other pathway had a markedly long residence time and therefore should be studied further to clarify the fate of carbon in the human body. In addition to excreta, data for serum and blood cell samples were also collected from the subjects to examine the metabolism of 13C in human body.

Seasonal accumulation of photoassimilated carbon relates to growth rate and use for new aboveground organs of young apple trees in following spring.

Deciduous trees accumulate carbon (C) in woody parts during the growth season which is subsequently used for the initial development and growth of newly formed organs in the following season; however, it is unclear which period during the growth season contributes to C accumulation. Three-year-old potted Malus domestica (apple) trees were grown in controlled growth chambers during the growth season and exposed to 13CO2 in an exposure chamber at seven different periods of the growth season, including vegetative and reproductive growth periods. Approximately half of the trees were harvested in late autumn, and the remaining trees were grown in a field in the following year. The 13C accumulation in the different organs in late autumn, and its concentration in the new aboveground growth during the following growth season, was determined. The concentration of the photoassimilated 13C in woody parts (shoots, trunk, rootstock and coarse roots) in the late autumn was higher in the trees labeled during the period of vigorous vegetative growth than in those labeled during other periods of growth. Furthermore, 13C concentration in the leaves, annual shoots, flower buds and flowers in the following early spring was also high in the trees labeled during this period. The concentration of 13C in the flower buds and flowers was positively correlated with that in the woody parts in the late autumn and old shoots in the following spring. Hence, the seasonal accumulation of photoassimilated C in woody parts in late autumn is related to growth rates during the growth season and its use for the initial development of newly formed organs in the following spring. These results suggest that under non-stressed conditions, C accumulated during the period of vigorous vegetative growth largely contributes to the C reserves that are used for the development of new organs in the following year.

DIRECT ASSIMILATION OF ATMOSPHERIC CARBON BY IMMATURE APPLE FRUITS.

Although fruit development primarily depends on photoassimilation by leaves, immature green fruits can also directly assimilate atmospheric CO2. To elucidate the process of C accumulation due to direct assimilation by fruit, we conducted a 13CO2 exposure experiment in an orchard in late June with immature 'Fuji' apples (Malus domestica). Four fruits from three trees were enclosed in transparent plastic bags and exposed to 13CO2 using an in-situ exposure system. Fruits were collected prior to and immediately following exposure in early July, late September and mid-November, and 13C concentrations in the peduncle, skin, flesh and core (including seeds) were measured. The higher assimilated 13C concentrations measured following exposure indicated that the fruits directly assimilated atmospheric 13C. The 13C concentration in fruit skin was higher immediately after exposure and in early July compared with that prior to exposure. In late September and mid-November, 13C concentrations were close to natural levels.

EXPERIMENTAL EVALUATION OF DISTRIBUTION OF 14C PHOTOASSIMILATED INTO CARBOHYDRATES IN DIFFERENT GROWTH STAGES OF FRUIT-BEARING APPLE SHOOTS USING A 13CO2 IN-SITU EXPOSURE SYSTEM.

In this study, we aimed to investigate the photoassimilation process of 14CO2 into agricultural plants through determining the photoassimilated carbohydrate-13C in each part (leaves, current branch and fruit) of the fruit-bearing apple shoots exposed to 13CO2 in different growth stages (early and late fruit development stages). The carbohydrate content was assessed as soluble (ethanol-extracted fraction) and other (HCl-extracted and residual fractions) components. The total (i.e. sum of the three fractions) bulk carbohydrate concentrations in all parts of the shoots were statistically similar between different growth stages. The changes in the concentration of 13C-labeled soluble carbohydrate (i.e. ethanol-extracted fraction) to the total content between different growth stages were statistically unclear among all parts of the shoot. These results suggest that the distribution ratio of photoassimilated 13C in soluble and other components in the apple shoot was thereabout constantly independent of the growth stages.

METABOLISMS OF 13C IN CATTLE SEMITENDINOSUS MUSCLE AFTER ADMINISTRATION OF 13C LABELED ORCHARD GRASS.

14C released from nuclear facilities is transferred to cattle through their consumption of 14C contaminated grasses. To estimate the concentrations of 14C in their meat, we conducted two sets of experiments. In the first experiment, 230 mg of 13C per day was administered to cattle aged 10 months for 28 days in the form of 13C-labeled grass. The 13C concentration in the semitendinosus muscle decreased exponentially after reaching its peak value. The mean half-life was 76 ± 13 days. In the second experiment, 550 mg of 13C per day was administered to 24-month-old cattle. The change in the semitendinosus muscle was smaller than that recorded in the first experiment, even though the amount of 13C administered per body weight was slightly higher than that in the first experiment. Consequently, the half-life was not determined. Therefore, further studies are required to clarify the metabolism of carbon in 2-year-old cattle.

DEVELOPMENT OF IN-SITU SYSTEMS FOR 13CO2 EXPOSURE AND DETERMINATION OF 13C NET ASSIMILATION RATE OF FRUIT-BEARING SHOOTS AND WHOLE TREE AND CHAMBER FOR PRECISION 13CO2 EXPOSURE OF YOUNG POTTED TREES.

14C is the most important radionuclide for assessing exposure dose around the Rokkasho nuclear fuel reprocessing plant, Aomori Prefecture, Japan. A simplified model with ample margins has been used so far for the assessment of the dose derived from 14C. Realistic dose estimate of 14C using more realistic model is necessary for safety. Apple production is an important core industry in Aomori Prefecture. To construct the dynamic model for apple, using 13C as a tracer to substitute for 14C, we collected data on 13C abundance in organs including fruit after 13CO2 exposure at various fruit growth stages. We developed 13CO2 exposure systems for three intact fruit-bearing shoots (1), whole mature tree (2) and young potted trees (3). Systems (1) and (2) also can determine net amounts of carbon and 13C photoassimilated. System (3) is capable of precise feedback control of 13CO2 and 12CO2 concentrations based on on-time determination.

In situ experimental exposure of fruit-bearing shoots of apple trees to 13CO2 and construction of a dynamic transfer model of carbon.

Evaluating the transfer and metabolism of carbon (C) in apple fruit is key to estimating the potential accumulation of atmospheric 14C in fruit near and around nuclear facilities. We developed a dynamic compartment model for apple fruit-bearing shoots, assuming that the shoots are a simple unit of source and sink for photoassimilates. Fruit-bearing shoots of Malus domestica "Fuji" at different fruit growth stages were exposed to 13CO2in situ, followed by sampling at 72 h after exposure or at harvest. The 13C/(13C+12C) mole ratio in fruits, leaves, and current branch were measured to construct a five-compartment model of 13C (fruit, each fast and slow component of leaves, and current branch). The C inventories in the compartments were presented in accordance with the measured growth curves of C in the organs. The model simulated the 13C dynamics in plant tissues well. Simulation results of photoassimilate distribution using the model indicated that the retention of photoassimilated C at the harvest depended on the growth rate of C in the organs at the exposure.

Short-term metabolism of biologically incorporated 125I ingested by olive flounder (Paralichthys olivaceus).

Iodine-129 with a long half-time of 1.6 × 107 y was discharged into the Pacific Ocean during the final safety tests of the first commercial nuclear fuel reprocessing plant in Japan, at Rokkasho, Aomori Prefecture. Olive flounder (Paralichthys olivaceus) is an important fishery along this coast. It is necessary to determine whether 129I accumulates in this species to assess the possible public acceptance. We developed a short-term metabolism model of 125I in the flounder using retention data for 1-6 days after the olive flounder had ingested a freshwater fish species, medaka (Oryzias latipes), that had been labeled with 125I by keeping them in water containing 125I for 7 days. A single compartment model constructed from whole-body retention data for 125I in the olive flounder, excluding the gastrointestinal tract and its contents, revealed a biological half-time of 2.9 days for 125I. When the gill and other tissues were separated to individual compartments, the biological half-time in the gill was three times longer than that in the other tissue, though the half-time in the gill is not statistically significant. The distribution of 125I among various tissues in the flounder 6 days after the ingestion of labeled medaka once a day for 6 days differed from that of stable I, suggesting that the biological half-time is longer in certain tissues. Further study is necessary to elucidate the metabolism of radioiodine in the flounder.

Estimation of dietary 14C dose coefficient using 13C-labelled compound administration analysis.

Carbon-14 released from nuclear facilities has been assessed to contribute significantly to the radiation dose that people are exposed to through the food chain. However, the current dose coefficient for members of public, which is the ratio of the 50-year committed effective dose to ingested 1 Bq 14C, recommended by the International Commission on Radiological Protection (ICRP) is not based on experimental human metabolic data for 14C in nutrients and diet. Therefore, to validate the coefficient, we administered 13C-labelled nutrients consisting of four amino acids, three fatty acids, and one monosaccharide to volunteers as substitutes for 14C labelled nutrients and measured the 13C concentration in various excreta samples. Although metabolic models were constructed from the excretion data, a significant fraction of administered 13C was not recovered from some nutrients. The dose coefficients of 14C in uniformly labelled Japanese diet, which were estimated under several assumptions about the unrecoverable fraction, varied from (6.2 ± 0.9) × 10-11 to (8.9 ± 4.4) × 10-10 Sv Bq-1 and were approximately comparable to the current value of 5.8 × 10-10 Sv Bq-1 recommended by the ICRP. Further studies are necessary to elucidate the metabolism of 14C in various nutrients in the unrecoverable fraction.

Development of a 1-week cycle menu for an Advanced Life Support System (ALSS) utilizing practical biomass production data from the Closed Ecology Experiment Facilities (CEEF).

Productivities of 29 crops in the Closed Ecology Experiment Facilities (CEEF) were measured. Rice and soybean showed higher productivities than these given by the Advanced Life Support System Modeling and Analysis Project Baseline Values and Assumption Document (BVAD), but productivities of some other crops, such as potato and sweet potato, were lower. The cultivation data were utilized to develop a 1-week cycle menu for Closed Habitation Experiment. The menu met most of the nutritional requirements. Necessary cultivation area per crew was estimated to be 255 m2. Results from this study can be used to help design the future Advanced Life Support System (ALSS) including the CEEF.

A dynamic transfer model for the estimation of 14C radioactivity in Japanese radish (Daikon) plants.

A dynamic compartment model was developed to describe C accumulation in the Japanese radish plant, which is an important crop in the area around Japan's first commercial nuclear fuel reprocessing plant in Rokkasho, Aomori, Japan. Photosynthetically fixed carbon is distributed into the leaf and the root compartments, and a part of the carbon accumulated in the leaf compartment is redistributed to the root compartment. The model parameters were estimated by using data obtained from exposure of the plant to CO2. The model estimates were in good agreement with the experimental observations, showing that the newly developed compartment model is applicable to assessment of the accumulation of C in Japanese radish plants around the nuclear facility. In this study, respiration rate was set to be proportional to the carbon mass of the compartment, though the respiration rate has been assumed generally to be proportional to the growth rate of the compartment. While the estimates using both respiration rates differed only slightly from each other, the ratio of the respiratory rate of the root to that of the leaf was too high in the case of the respiratory rate proportional to the growth rate.

Development of a dynamic transfer model of (14)C from the atmosphere to rice plants.

A dynamic compartment model was investigated to describe (14)C accumulation in rice plants exposed to atmospheric (14)C with temporally changing concentrations. In the model, rice plants were regarded to consist of three compartments: the ear and the mobile and immobile carbon pools of the shoot. Photosynthetically fixed carbon moves into the ear and the mobile carbon pool, and these two compartments release a part of this carbon into the atmosphere by respiration. Carbon accumulated in the mobile carbon pool is redistributed to the ear, while carbon transferred into the immobile carbon pool from the mobile one is accumulated there until harvest. The model was examined by cultivation experiments using the stable isotope, (13)C, in which the ratios of carbon photosynthetically fixed at nine times during plant growth to the total carbon at the time of harvest were determined. The model estimates of the ratios were in relatively good agreement with the experimental observations, which implies that the newly developed compartment model is applicable to estimate properly the radiation dose to the neighboring population due to an accidental release of (14)C from nuclear facilities.