RESUMO
Zymomonas mobilis is an ethanologenic bacterium that has been studied for use in biofuel production. Of the sequenced Zymomonas strains, ATCC 29191 has been described as the phenotypic centrotype of Zymomonas mobilis subsp. mobilis, the taxon that harbors the highest ethanol-producing Z. mobilis strains. ATCC 29191 was isolated in Kinshasa, Congo, from palm wine fermentations. This strain is reported to be a robust levan producer, while in recent years it has been employed in studies addressing Z. mobilis respiration. Here we announce the finishing and annotation of the ATCC 29191 genome, which comprises one chromosome and three plasmids.
Assuntos
DNA Bacteriano/química , DNA Bacteriano/genética , Etanol/metabolismo , Genoma Bacteriano , Análise de Sequência de DNA , Zymomonas/genética , Zymomonas/metabolismo , Congo , Fermentação , Frutanos/metabolismo , Redes e Vias Metabólicas , Dados de Sequência Molecular , Oxirredução , Plasmídeos , Vinho/microbiologia , Zymomonas/isolamento & purificaçãoRESUMO
Rahnella aquatilis CIP 78.65 is a gammaproteobacterium isolated from a drinking water source in Lille, France. Here we report the complete genome sequence of Rahnella aquatilis CIP 78.65, the type strain of R. aquatilis.
Assuntos
Água Potável/microbiologia , Genoma Bacteriano , Rahnella/genética , Sequência de Bases , França , Dados de Sequência Molecular , Rahnella/classificação , Rahnella/isolamento & purificaçãoRESUMO
Rahnella sp. strain Y9602 is a gammaproteobacterium isolated from contaminated subsurface soils that is capable of promoting uranium phosphate mineralization as a result of constitutive phosphatase activity. Here we report the first complete genome sequence of an isolate belonging to the genus Rahnella.
Assuntos
Genoma Bacteriano , Metais/química , Radioisótopos/química , Rahnella/genética , Microbiologia do Solo , Sequência de Bases , Dados de Sequência Molecular , Rahnella/classificação , Poluentes do Solo/químicaRESUMO
We announce the availability of a high-quality draft of the genome sequence of Amycolatopsis sp. strain 39116, one of few bacterial species that are known to consume the lignin component of plant biomass. This genome sequence will further ongoing efforts to use microorganisms for the conversion of plant biomass into fuels and high-value chemicals.
Assuntos
Actinobacteria/classificação , Actinobacteria/genética , Biomassa , Genoma Bacteriano , Plantas , Actinobacteria/metabolismo , Biodegradação Ambiental , Cromossomos Bacterianos , DNA Bacteriano/genética , Regulação Bacteriana da Expressão Gênica , Dados de Sequência MolecularRESUMO
Microbial communities in the termite hindgut are essential for degrading plant material. We present the high-quality draft genome sequence of the Opitutaceae bacterium strain TAV1, the first member of the phylum Verrucomicrobia to be isolated from wood-feeding termites. The genomic analysis reveals genes coding for lignocellulosic degradation and nitrogen fixation.
Assuntos
Genoma Bacteriano , Isópteros/microbiologia , Simbiose/fisiologia , Verrucomicrobia/genética , Animais , Proteínas de Bactérias/genética , Proteínas de Bactérias/metabolismo , Regulação Bacteriana da Expressão Gênica/fisiologia , Dados de Sequência MolecularRESUMO
Marinitoga piezophila KA3 is a thermophilic, anaerobic, chemoorganotrophic, sulfur-reducing bacterium isolated from the Grandbonum deep-sea hydrothermal vent site at the East Pacific Rise (13°N, 2,630-m depth). The genome of M. piezophila KA3 comprises a 2,231,407-bp circular chromosome and a 13,386-bp circular plasmid. This genome was sequenced within Department of Energy Joint Genome Institute CSP 2010.
Assuntos
Bactérias/genética , DNA Bacteriano/química , DNA Bacteriano/genética , Genoma Bacteriano , Análise de Sequência de DNA , Anaerobiose , Bactérias/crescimento & desenvolvimento , Bactérias/isolamento & purificação , Bactérias/metabolismo , Cromossomos Bacterianos , Fontes Hidrotermais/microbiologia , Dados de Sequência Molecular , Compostos Orgânicos/metabolismo , Oxirredução , Oceano Pacífico , Plasmídeos , Água do Mar/microbiologia , Enxofre/metabolismo , TemperaturaRESUMO
Desulfosporosinus species are sulfate-reducing bacteria belonging to the Firmicutes. Their genomes will give insights into the genetic repertoire and evolution of sulfate reducers typically thriving in terrestrial environments and able to degrade toluene (Desulfosporosinus youngiae), to reduce Fe(III) (Desulfosporosinus meridiei, Desulfosporosinus orientis), and to grow under acidic conditions (Desulfosporosinus acidiphilus).
Assuntos
Genoma Bacteriano , Peptococcaceae/classificação , Peptococcaceae/genética , DNA Bacteriano/genética , Dados de Sequência Molecular , Especificidade da EspécieRESUMO
BACKGROUND: Natrialba magadii is an aerobic chemoorganotrophic member of the Euryarchaeota and is a dual extremophile requiring alkaline conditions and hypersalinity for optimal growth. The genome sequence of Nab. magadii type strain ATCC 43099 was deciphered to obtain a comprehensive insight into the genetic content of this haloarchaeon and to understand the basis of some of the cellular functions necessary for its survival. RESULTS: The genome of Nab. magadii consists of four replicons with a total sequence of 4,443,643 bp and encodes 4,212 putative proteins, some of which contain peptide repeats of various lengths. Comparative genome analyses facilitated the identification of genes encoding putative proteins involved in adaptation to hypersalinity, stress response, glycosylation, and polysaccharide biosynthesis. A proton-driven ATP synthase and a variety of putative cytochromes and other proteins supporting aerobic respiration and electron transfer were encoded by one or more of Nab. magadii replicons. The genome encodes a number of putative proteases/peptidases as well as protein secretion functions. Genes encoding putative transcriptional regulators, basal transcription factors, signal perception/transduction proteins, and chemotaxis/phototaxis proteins were abundant in the genome. Pathways for the biosynthesis of thiamine, riboflavin, heme, cobalamin, coenzyme F420 and other essential co-factors were deduced by in depth sequence analyses. However, approximately 36% of Nab. magadii protein coding genes could not be assigned a function based on Blast analysis and have been annotated as encoding hypothetical or conserved hypothetical proteins. Furthermore, despite extensive comparative genomic analyses, genes necessary for survival in alkaline conditions could not be identified in Nab. magadii. CONCLUSIONS: Based on genomic analyses, Nab. magadii is predicted to be metabolically versatile and it could use different carbon and energy sources to sustain growth. Nab. magadii has the genetic potential to adapt to its milieu by intracellular accumulation of inorganic cations and/or neutral organic compounds. The identification of Nab. magadii genes involved in coenzyme biosynthesis is a necessary step toward further reconstruction of the metabolic pathways in halophilic archaea and other extremophiles. The knowledge gained from the genome sequence of this haloalkaliphilic archaeon is highly valuable in advancing the applications of extremophiles and their enzymes.
Assuntos
Genômica , Halobacteriaceae/genética , Proteínas Arqueais/genética , Proteínas Arqueais/metabolismo , Mapeamento Cromossômico , Cromossomos de Archaea , Coenzimas/biossíntese , Genoma ArquealRESUMO
Zymomonas mobilis is an alphaproteobacterium studied for bioethanol production. Different strains of this organism have been hitherto sequenced; they all belong to the Z. mobilis subsp. mobilis taxon. Here we report the finished and annotated genome sequence of strain ATCC 29192, a cider-spoiling agent isolated in the United Kingdom. ATCC 29192 is the lectotype of the second-best-characterized subspecies of Z. mobilis, Z. mobilis subsp. pomaceae. The nucleotide sequence of ATCC 29192 deviates from that of Z. mobilis subsp. mobilis representatives, which justifies its distinct taxonomic positioning and proves particularly useful for comparative and functional genomic analyses.
Assuntos
DNA Bacteriano/química , DNA Bacteriano/genética , Genoma Bacteriano , Análise de Sequência de DNA , Zymomonas/genética , Etanol/metabolismo , Microbiologia de Alimentos , Dados de Sequência Molecular , Reino Unido , Zymomonas/isolamento & purificação , Zymomonas/metabolismoRESUMO
Glaciecola sp. strain 4H-3-7+YE-5 was isolated from subseafloor sediments at Suruga Bay in Japan and is capable of efficiently hydrolyzing cellulose and xylan. The complete genome sequence of Glaciecola sp. 4H-3-7+YE-5 revealed several genes encoding putatively novel glycoside hydrolases, offering a high potential for plant biomass degradation.
Assuntos
Alteromonadaceae/genética , Alteromonadaceae/isolamento & purificação , Celulose/metabolismo , Genoma Bacteriano , Xilanos/metabolismo , Alteromonadaceae/metabolismo , Biodegradação Ambiental , Sedimentos Geológicos/microbiologia , Glicosídeo Hidrolases/genética , Glicosídeo Hidrolases/metabolismo , Hidrolases/genética , Japão , Dados de Sequência MolecularRESUMO
Nitrosomonas sp. strain AL212 is an obligate chemolithotrophic ammonia-oxidizing bacterium (AOB) that was originally isolated in 1997 by Yuichi Suwa and colleagues. This organism belongs to Nitrosomonas cluster 6A, which is characterized by sensitivity to high ammonia concentrations, higher substrate affinity (lower K(m)), and lower maximum growth rates than strains in Nitrosomonas cluster 7, which includes Nitrosomonas europaea and Nitrosomonas eutropha. Genome-informed studies of this ammonia-sensitive cohort of AOB are needed, as these bacteria are found in freshwater environments, drinking water supplies, wastewater treatment systems, and soils worldwide.
Assuntos
DNA Bacteriano/química , DNA Bacteriano/genética , Genoma Bacteriano , Nitrosomonas/genética , Análise de Sequência de DNA , Amônia/metabolismo , Crescimento Quimioautotrófico , Dados de Sequência Molecular , Nitrosomonas/isolamento & purificação , Nitrosomonas/metabolismo , Oxirredução , PlasmídeosRESUMO
Kosmotoga olearia strain TBF 19.5.1 is a member of the Thermotogales that grows best at 65°C and very well even at 37°C. Information about this organism is important for understanding the evolution of mesophiles from thermophiles. Its genome sequence reveals extensive gene gains and a large content of mobile genetic elements. It also contains putative hydrogenase genes that have no homologs in the other member of the Thermotogales.
Assuntos
Bactérias/genética , Bactérias/crescimento & desenvolvimento , Dados de Sequência Molecular , Mar do Norte , Petróleo/microbiologia , TemperaturaRESUMO
Streptomyces griseus strain XylebKG-1 is an insect-associated strain of the well-studied actinobacterial species S. griseus. Here, we present the genome of XylebKG-1 and discuss its similarity to the genome of S. griseus subsp. griseus NBRC13350. XylebKG-1 was isolated from the fungus-cultivating Xyleborinus saxesenii system. Given its similarity to free-living S. griseus subsp. griseus NBRC13350, comparative genomics will elucidate critical components of bacterial interactions with insects.
Assuntos
DNA Bacteriano/química , DNA Bacteriano/genética , Genoma Bacteriano , Análise de Sequência de DNA , Streptomyces griseus/genética , Animais , Dados de Sequência Molecular , Streptomyces griseus/isolamento & purificação , Gorgulhos/microbiologiaRESUMO
Clostridium thermocellum DSM1313 is a thermophilic, anaerobic bacterium with some of the highest rates of cellulose hydrolysis reported. The complete genome sequence reveals a suite of carbohydrate-active enzymes and demonstrates a level of diversity at the species level distinguishing it from the type strain ATCC 27405.
Assuntos
Clostridium thermocellum/genética , DNA Bacteriano/química , DNA Bacteriano/genética , Genoma Bacteriano , Análise de Sequência de DNA , Anaerobiose , Celulose/metabolismo , Clostridium thermocellum/metabolismo , Clostridium thermocellum/fisiologia , Temperatura Alta , Dados de Sequência MolecularRESUMO
Cellulosilyticum lentocellum DSM 5427 is an anaerobic, endospore-forming member of the Firmicutes. We describe the complete genome sequence of this cellulose-degrading bacterium, which was originally isolated from estuarine sediment of a river that received both domestic and paper mill waste. Comparative genomics of cellulolytic clostridia will provide insight into factors that influence degradation rates.
Assuntos
Celulose/metabolismo , Genoma Bacteriano , Bacilos Gram-Positivos Formadores de Endosporo/classificação , Bacilos Gram-Positivos Formadores de Endosporo/genética , Celulose/química , Sedimentos Geológicos/microbiologia , Resíduos Industriais , Anotação de Sequência Molecular , Dados de Sequência Molecular , Papel , RNA Bacteriano/genética , Rios , Eliminação de Resíduos Líquidos , Poluentes Químicos da ÁguaRESUMO
Desulfovibrio alaskensis G20 (formerly Desulfovibrio desulfuricans G20) is a Gram-negative mesophilic sulfate-reducing bacterium (SRB), known to corrode ferrous metals and to reduce toxic radionuclides and metals such as uranium and chromium to sparingly soluble and less toxic forms. We present the 3.7-Mb genome sequence to provide insights into its physiology.
Assuntos
Desulfovibrio/classificação , Desulfovibrio/genética , Genoma Bacteriano , Sequência de Bases , Desulfovibrio/fisiologia , Dados de Sequência MolecularRESUMO
Two members of the family Flavobacteriaceae were isolated from subseafloor sediments using artificial seawater with cellulose, xylan, and chitin as the sole carbon and energy sources. Here, we present the complete genome sequences of Krokinobacter sp. strain 4H-3-7-5 and Lacinutrix sp. strain 5H-3-7-4, which both encode putatively novel enzymes involved in cellulose, hemicellulose, and chitin metabolism.
Assuntos
Flavobacteriaceae/genética , Flavobacteriaceae/isolamento & purificação , Genoma Bacteriano , Biodegradação Ambiental , Celulose/metabolismo , Quitina/metabolismo , Flavobacteriaceae/enzimologia , Sedimentos Geológicos/microbiologia , Dados de Sequência Molecular , Polissacarídeos/metabolismo , Água do Mar/microbiologiaRESUMO
Alicycliphilus denitrificans strain BC and A. denitrificans strain K601(T) degrade cyclic hydrocarbons. These strains have been isolated from a mixture of wastewater treatment plant material and benzene-polluted soil and from a wastewater treatment plant, respectively, suggesting their role in bioremediation of soil and water. Although the strains are phylogenetically closely related, there are some clear physiological differences. The hydrocarbon cyclohexanol, for example, can be degraded by strain K601(T) but not by strain BC. Furthermore, both strains can use nitrate and oxygen as an electron acceptor, but only strain BC can use chlorate as electron acceptor. To better understand the nitrate and chlorate reduction mechanisms coupled to the oxidation of cyclic compounds, the genomes of A. denitrificans strains BC and K601(T) were sequenced. Here, we report the complete genome sequences of A. denitrificans strains BC and K601(T).
Assuntos
Comamonadaceae/genética , DNA Bacteriano/química , DNA Bacteriano/genética , Genoma Bacteriano , Análise de Sequência de DNA , Biotransformação , Cloratos , Comamonadaceae/isolamento & purificação , Comamonadaceae/metabolismo , Hidrocarbonetos Cíclicos/metabolismo , Dados de Sequência Molecular , Nitratos/metabolismo , Microbiologia do Solo , Poluentes do Solo/metabolismo , Microbiologia da Água , Poluentes Químicos da Água/metabolismoRESUMO
Ruminococcus albus 7 is a highly cellulolytic ruminal bacterium that is a member of the phylum Firmicutes. Here, we describe the complete genome of this microbe. This genome will be useful for rumen microbiology and cellulosome biology and in biofuel production, as one of its major fermentation products is ethanol.
Assuntos
Genoma Bacteriano/genética , Ruminococcus/genéticaRESUMO
Thermotoga sp. strain RQ2 is probably a strain of Thermotoga maritima. Its complete genome sequence allows for an examination of the extent and consequences of gene flow within Thermotoga species and strains. Thermotoga sp. RQ2 differs from T. maritima in its genes involved in myo-inositol metabolism. Its genome also encodes an apparent fructose phosphotransferase system (PTS) sugar transporter. This operon is also found in Thermotoga naphthophila strain RKU-10 but no other Thermotogales. These are the first reported PTS transporters in the Thermotogales.