RESUMO
A method for the determination of oestriol in pregnancy urine by normal-phase high-performance liquid chromatography with electrochemical detection is described. A large-volume wall-jet cell with an Ag-Ag+ reference electrode was used as the detector system. The limit of detection obtained is comparable to that of electrochemical detection following reversed-phase liquid chromatography. One of the advantages of electrochemical detection with normal-phase systems is that adsorption problems are minimized.
Assuntos
Estriol/urina , Gravidez , Fenômenos Químicos , Química , Cromatografia Líquida de Alta Pressão/métodos , Eletroquímica , Eletrodos , Estradiol/urina , Estrona/urina , Feminino , Humanos , Hidrólise , Masculino , Espectrometria de FluorescênciaRESUMO
Ambient air particulates were collected using high volume samplers at seven locations throughout Singapore. The particulates were analyzed for eleven polynuclear aromatic hydrocarbons (PAH) using high performance liquid chromatography (HPLC) with fluorescence detection. Several sample preparation techniques were used to determine which method would yield the most PAHs from the bulk sample matrix. PAH profiles (standardised against the concentration of benzo(a)pyrene) were obtained to characterise the different sampling sites chosen.