RESUMO
Morphological sperm evaluation supported by the morphometry can be used in the determination of the seminal quality and in the investigation of potential extenders. Although there are studies comparing TRIS and ACP extenders, there are no comparative studies between them for the computerized assisted semen analysis (CASA), sperm viability, membrane functionality and sperm morphometry parameters of cryopreserved canine semen. Hence, we aimed to evaluate the effects of ACP-106c and TRIS on post-freezing canine sperm quality. Five dogs were submitted to semen collection twice with one-week interval. The semen was evaluated within the parameters: total motility, vigor, concentration, viability, plasma membrane functionality, morphology and morphometry. In the morphometric evaluation, the morphologically normal sperm was measured as: length, width, area and perimeter of the head and the midpiece, tail length and total length. The parameters of ellipticity, elongation, regularity and roughness were determined. Then, the semen was divided into two aliquots that were diluted in TRIS or ACP-106c, with the addition of egg yolk and glycerol. The diluted semen was refrigerated and frozen. The thawed samples were evaluated. Total motility, viability, sperm membrane functionality and normal morphology reduced after thawing in both extenders (morphology reduced from 89.60 ± 1.3% to 84.40 ± 1.8 and 84.60 ± 1.1% in TRIS and ACP-106c, respectively). However, it did not differ between TRIS and ACP-106c. In the ACP-106c the sperm head defects in cryopreserved semen were higher compared to fresh semen (P < 0.05). For all the morphometric parameters evaluated, there were no differences between fresh and cryopreserved samples (3.70 ± 0.4% vs. 2.30 ± 0.5%). In kinetics, with an interval of one week statistical differences between the extenders were found only in the parameters ALH and LIN (P < 0.05). Regardless of the extender, there were no changes in the morphometric parameters of sperm after thawing.
RESUMO
Introdução: A doença arterial coronariana (DAC) constitui a principal causa de morbi-mortalidade no mundo moderno. A ecocardiografia sob estresse tem desempenhado um papel central no diagnóstico não invasivo da isquemia e viabilidade miocárdica. Em indivíduos com capacidade física preservada o esforço físico é o indutor de isquemia miocárdica de primeira escolha. A ecocardiografia sob estresse pelo esforço físico(EEEF) tem mostrado acurácia diagnóstica superior à do teste ergométrico. O objetivo deste estudo é determinar o valor preditivo positivo (VPP) desta metodologia, considerando como, padrão ouro, os parêmetros obtidos mediante (CACG). Métodos: Trata-se de estudo observacional, no qual foram avaliados 3861 pacientes, no período de 12/2000 a 08/2006, sendo 53 por cento mulheres com idade de 55 mais ou menos 10 anos(média mais ou menos desvio padrão). A EEEF foi realizada em esteira ergométrica com protocolo de Bruce. O CACG foi efetuado mediante a técnica de Judkins, preferencialmente por via femural direita. Resultados: A EEEF foi normal em 2710 (69,9 por cento) pacientes, e positiva para isquemia miocárdica em 1151 (30,1 por cento)...