Isolating Specific vs. Non-Specific Binding Responses in Conducting Polymer Biosensors for Bio-Fingerprinting.

A longstanding challenge for accurate sensing of biomolecules such as proteins concerns specifically detecting a target analyte in a complex sample (e.g., food) without suffering from nonspecific binding or interactions from the target itself or other analytes present in the sample. Every sensor suffers from this fundamental drawback, which limits its sensitivity, specificity, and longevity. Existing efforts to improve signal-to-noise ratio involve introducing additional steps to reduce nonspecific binding, which increases the cost of the sensor. Conducting polymer-based chemiresistive biosensors can be mechanically flexible, are inexpensive, label-free, and capable of detecting specific biomolecules in complex samples without purification steps, making them very versatile. In this paper, a poly (3,4-ethylenedioxyphene) (PEDOT) and poly (3-thiopheneethanol) (3TE) interpenetrating network on polypropylene-cellulose fabric is used as a platform for a chemiresistive biosensor, and the specific and nonspecific binding events are studied using the Biotin/Avidin and Gliadin/G12-specific complementary binding pairs. We observed that specific binding between these pairs results in a negative ΔR with the addition of the analyte and this response increases with increasing analyte concentration. Nonspecific binding was found to have the opposite response, a positive ΔR upon the addition of analyte was seen in nonspecific binding cases. We further demonstrate the ability of the sensor to detect a targeted protein in a dual-protein analyte solution. The machine-learning classifier, random forest, predicted the presence of Biotin with 75% accuracy in dual-analyte solutions. This capability of distinguishing between specific and nonspecific binding can be a step towards solving the problem of false positives or false negatives to which all biosensors are susceptible.

Anisotropy of W-band complex permittivity in Al2O3.

The dielectric anisotropy of Al2O3 is studied here by characterizing W-band (75-110 GHz) complex permittivity of four different orientations of sapphire (Al2O3 single crystals). This was done using free-space, focused beam methods. Dielectric polarizability ([Formula: see text]) of these orientations is then calculated and these values are related to their complex permittivity. Based on this relationship, a framework is developed for rapid and straightforward estimation of dielectric anisotropy using a known crystal structure and a dielectric permittivity measurement performed on one orientation of the material. This framework can be applied to other materials with dielectric anisotropy (e.g. SnO2, LiGaO2) to predict permittivity for different orientations, enabling rapid design of high-frequency systems (e.g. radomes, electromagnetic windows). These permittivity measurements were also used to determine the dominant polarization mechanisms leading to dielectric anisotropy of Al2O3 in the W-band; electronic and ionic polarization orthogonal to the direction of the focused beam.