RESUMO
Pectin lyases are important in various industries, including tobacco leaves processing. In this paper, a novel pectin lyase Pel04 from Bacillus velezensis was characterized. Pel04 molecular weight (Mw) and isoelectric point (pI) of the protein sequence after removing the signal peptide are 43.0 kDa. The optimal temperature and pH of Pel04 is 50 °C and 9.0, respectively. Pel04 was stable in the range of 30-50 °C, and pH 9.5-11. Ca2+ can significantly stimulate the enzyme activity, while Cu2+, Co2+, Fe3+, and Mn2+ have inhibitory effects on Pel04. By Pel04 treatment, the overall content of acids, alcohols, esters and other aromas in tobacco leaves increased, while the contents of phenolic and heterocyclic substances decreased. Pel04 has important potential for industrial application particularly in improving quality of tobacco leaves.
RESUMO
OBJECTIVE: To identify the predictive factors associated with pleural drainage volume (PDV) after uniportal video-assisted thoracic surgery (VATS) lobectomy for non-small cell lung cancer (NSCLC). METHODS: A total of 440 consecutive NSCLC patients who underwent uniportal VATS lobectomy were enrolled in this study between November 2016 and July 2019. Thirty-four parameters, including patients' clinicopathological characteristics and other potential predictors were collected. Daily drainage volume was summed up as PDV. Univariate analysis and multivariate regression models were fitted to identify independent predictive factors for PDV. RESULTS: The median PDV was 840 ml during the median drainage duration of 4 days. A strong correlation was observed between PDV and drainage duration (correlation coefficient = 0.936). On univariate analysis, age, forced expiratory volume in 1 s % predicted (FEV1%), left ventricular ejection fraction (LVEF), operation time, serum total protein (TP), and body mass index (BMI) showed a significant correlation with PDV (P value, < 0.001, < 0.001, 0.003, 0.008, 0.028, and 0.045, respectively). Patients with smoking history (P = 0.030) or who underwent lower lobectomy (P = 0.015) showed significantly increased PDV than never smokers or those who underwent upper or middle lobectomy, respectively. On multivariate regression analysis, older age (P< 0.001), lower FEV1% (P< 0.001), lower LVEF (P = 0.011), lower TP (P = 0.013), and lower lobectomy (P = 0.016) were independent predictors of increased PDV. CONCLUSIONS: Predictive factors of PDV can be identified. Based on these predictors, patients can be treated with tailored individualized safe chest tube management.
Assuntos
Carcinoma Pulmonar de Células não Pequenas , Neoplasias Pulmonares , Idoso , Carcinoma Pulmonar de Células não Pequenas/cirurgia , Drenagem , Humanos , Neoplasias Pulmonares/cirurgia , Pneumonectomia , Complicações Pós-Operatórias/epidemiologia , Prognóstico , Estudos Retrospectivos , Volume Sistólico , Cirurgia Torácica Vídeoassistida , Função Ventricular EsquerdaRESUMO
Cytoglobin (Cygb), a recently discovered member of the vertebrate globin family, exhibits a traditional globin fold with a three-over-three α-helical sandwich. The interaction between copper(â ¡) ion (Cu2+) and Cygb has been investigated by UV-Vis, fluorescence, synchronous fluorescence and circular dichroism (CD) spectra. Results showed that the absorption intensity of Cygb at 280 nm increased and the intrinsic fluorescence of Cygb was quenched when Cu2+ was added. This fluorescence quenching of Cygb has been proven that it belongs to static quenching. The synchronous fluorescence spectra indicated that there were small changes about the microenvironment of tryptophan residues and tyrosine residues; furthermore, the binding site of Cu2+ is closer to tryptophan residues than tyrosine residues. No obvious change was observed about the secondary structure of Cygb with the addition of Cu2+ from the CD spectra.