RESUMO
Steroidal saponins are a class of specialized metabolites essential for plant's response to biotic and abiotic stresses. They are also important raw materials for the industrial production of steroid drugs. Steroidal saponins are present in some monocots, such as Dioscorea and Paris, but their distribution, origin, and evolution in plants remain poorly understood. By reconstructing the evolutionary history of the steroidal saponin-associated module (SSAM) in plants, we reveal that the steroidal saponin pathway has its origin in Asparagus and Dioscorea. Through evaluating the distribution and evolutionary pattern of steroidal saponins in angiosperms, we further show that steroidal saponins originated multiple times in angiosperms, and exist in early diverged lineages of certain monocot lineages including Asparagales, Dioscoreales, and Liliales. In these lineages, steroidal saponins are synthesized through the high copy and/or high expression mechanisms of key genes in SSAM. Together with shifts in gene evolutionary rates and amino acid usage, these molecular mechanisms shape the current distribution and diversity of steroidal saponins in plants. Consequently, our results provide new insights into the distribution, diversity and evolutionary history of steroidal saponins in plants, and enhance our understanding of plants' resistance to abiotic and biotic stresses. Additionally, fundamental understanding of the steroidal saponin biosynthesis will facilitate their industrial production and pharmacological applications.
Assuntos
Plantas , Saponinas , Plantas/metabolismoRESUMO
At present, cometabolic degradation is an extensive method for the biological removal of high molecular weight polycyclic aromatic hydrocarbons (HMW-PAHs) in the marine environment. However, due to the refractory to degradation and high toxicity, there are few studies on pyrene (PYR) cometabolic degradation with phenanthrene (PHE) as substrate. In this study, a Pseudomonas stutzeri DJP1 strain isolated from sediments was used in the cometabolic system of PHE and PYR. The biomass and the activity of key enzymes such as dehydrogenase and catechol 12 dioxygenase of strain were improved, but the enhancement of biotoxicity resulted in the inhibition of cometabolism simultaneously. Seven metabolites were identified respectively in PYR, PHE degradation cultures. It was speculated that the cometabolism of PHE and PYR had a common phthalic acid pathway, and the degradation pathway of PHE was included in the downstream pathway of PYR. The functional genes such as PhdF, NidD and CatA involved in DJP1 degradation were revealed by Genome analysis. This study provides a reference for the biodegradation of PYR and PHE in real marine environment.
Assuntos
Fenantrenos , Hidrocarbonetos Policíclicos Aromáticos , Pseudomonas stutzeri , Pseudomonas stutzeri/genética , Pseudomonas stutzeri/metabolismo , Fenantrenos/metabolismo , Hidrocarbonetos Policíclicos Aromáticos/metabolismo , Pirenos/metabolismo , Biodegradação AmbientalRESUMO
Ensuring the safe production of food and oil crops in soils with elevated cadmium (Cd) content in karst regions is crucial. We tested a field experiment to examine the long-term remediation effects of compound microorganisms (CM), strong anion exchange adsorbent (SAX), processed oyster shell (POS), and composite humic acids (CHA) on Cd contamination in paddy fields under a rice-oilseed rape rotation system. In comparison to the control group (CK), the application of amendments significantly increased soil pH, cation exchange capacity (CEC), and soil organic matter (SOM) content while markedly decreasing the content of available Cd (ACd). During the rice cultivation season, Cd was predominantly concentrated in the roots. Relative to the control (CK), the Cd content in each organ was significantly reduced. The Cd content in brown rice decreased by 19.18-85.45%. The Cd content in brown rice following different treatments exhibited the order of CM > POS > CHA > SAX, which was lower than the Chinese Food Safety Standard (GB 2762-2017) (0.20 mg/kg). Intriguingly, during the oilseed rape cultivation season, we discovered that oilseed rape possesses potential phytoremediation capabilities, with Cd mainly accumulating in roots and stems. Notably, CHA treatment alone significantly decreased the Cd content in oilseed rape grains to 0.156 mg/kg. CHA treatment also maintained soil pH and SOM content, consistently reduced soil ACd content, and stabilized Cd content in RSF within the rice-oilseed rape rotation system. Importantly, CHA treatment not only enhances crop production but also has a low total cost (1255.230 US$/hm2). Our research demonstrated that CHA provides a consistent and stable remediation effect on Cd-contaminated rice fields within the crop rotation system, as evidenced by the analysis of Cd reduction efficiency, crop yield, soil environmental change, and total cost. These findings offer valuable guidance for sustainable soil utilization and safe production of grain and oil crops in the context of high Cd concentrations in karst mountainous regions.
Assuntos
Brassica napus , Cádmio , Oryza , Poluentes do Solo , Cádmio/toxicidade , Carbonato de Cálcio , Produtos Agrícolas , Substâncias Húmicas/análise , Solo/química , Poluentes do Solo/análiseRESUMO
Cadmium (Cd) is a toxic metal that threatens human health when enriched in crops. NRAMPs are a family of natural macrophage proteins reported to play a key role in Cd transport in plants. In order to explore the gene regulation mechanism of potato under Cd stress and the role of NRAMPs family in it, this study analyzed the gene expression differences of two different Cd accumulation levels in potato after 7 days of 50 mg/kg Cd stress and screened out the key genes that may play a major role in the differential accumulation of Cd in different varieties. Additionally, StNRAMP2 was selected for verification. Further verification showed that the StNRAMP2 gene plays an important role in the accumulation of Cd in potato. Interestingly, silencing StNRAMP2 increased Cd accumulation in tubers but significantly decreased Cd accumulation in other sites, suggesting a critical role of StNRAMP2 in Cd uptake and transport in potatoes. To further confirm this conclusion, we performed heterologous expression experiments in which overexpression of StNRAMP2 gene in tomato resulted in a threefold increase in Cd content, which further confirmed the important role of StNRAMP2 in the process of Cd accumulation compared with wild-type plants. In addition, we found that the addition of Cd to the soil increased the activity of the plant antioxidant enzyme system, and silencing StNRAMP2 partially reversed this effect. This suggests that the StNRAMP2 gene plays an important role in plant stress tolerance, and future studies could further explore the role of this gene in other environmental stresses. In conclusion, the results of this study improve the understanding of the mechanism of Cd accumulation in potato and provide experimental basis for remediation of Cd pollution.
Assuntos
Poluentes do Solo , Solanum tuberosum , Transporte Biológico , Cádmio/toxicidade , Tubérculos/metabolismo , Solo , Poluentes do Solo/metabolismo , Solanum tuberosum/genética , Solanum tuberosum/metabolismo , Genes de PlantasRESUMO
Multidrug resistance (MDR) is a major obstacle to effective cancer treatment. Therefore, developing effective approaches for overcoming the limitation of MDR in cancer therapy is very essential. Chemotherapy combined with photothermal therapy (PTT) is a potential therapeutic option against MDR. Herein, we developed a subcellular-targeted near-infrared (NIR)-responsive nanomedicine (Fe3O4@PDA-TPP/S2-PEG-hyd-DOX, abbreviated as Fe3O4-ATSPD) as a new photothermal agent with improved photothermal stability and efficiency. This system demonstrates high stability in blood circulation and can be accumulated at the tumor site by magnetic targeting enhanced permeability and retention effect (EPR). Near-infrared (NIR) irradiation at the tumor site generates a photothermal effect from the photosensitizer Fe3O4@PDA, leading to a dramatic decrease in mitochondrial membrane potential. Simultaneously, the conjugated drugs released under low pH condition in endosomes or lysosomes cause nucleus DNA damage and cell apoptosis. This subcellular-targeted NIR-responsive nanomedicine with efficient integration of diagnosis and therapy could significantly enhance MDR cancer treatment by combination of chemotherapy and PTT.
Assuntos
Nanopartículas , Neoplasias , Humanos , Terapia Fototérmica , Doxorrubicina/farmacologia , Doxorrubicina/uso terapêutico , Nanomedicina , Resistência a Múltiplos Medicamentos , Resistencia a Medicamentos Antineoplásicos , Neoplasias/tratamento farmacológico , Neoplasias/patologia , FototerapiaRESUMO
Paris species accumulate a large amount of steroidal saponins, which have numerous pharmacological activities and have become an essential component in many patented drugs. However, only two among all Paris species. Paris are identified as official sources due to high level of bioactive compounds. To clarify the composition of steroidal saponins and the molecular basis behind the differences between species, we investigated transcriptome and metabolic profiles of leaves and rhizomes in Paris polyphylla var. chinensis (PPC), Paris polyphylla var. yunnanensis (PPY), Paris polyphylla var. stenophylla (PPS), Paris fargesii (PF), and Paris mairei (PM). Phytochemical results displayed that the accumulation of steroidal saponins was tissue- and species-specific. PF and PPS contained more steroidal saponins in leaves than rhizomes, while PPY accumulated more steroidal saponins in rhizomes than leaves. PPC and PM contained similar amounts of steroidal saponins in leaves and rhizomes. Transcriptome analysis illustrated that most differentially expressed genes related to the biosynthesis of steroidal saponins were abundantly expressed in rhizomes than leaves. Meanwhile, more biosynthetic genes had significant correlations with steroidal saponins in rhizomes than in leaves. The result of CCA indicated that ACAT, DXS, DWF1, and CYP90 constrained 97.35% of the variance in bioactive compounds in leaves, whereas CYP72, UGT73, ACAT, and GPPS constrained 98.61% of the variance in phytochemicals in rhizomes. This study provided critical information for enhancing the production of steroidal saponins by biotechnological approaches and methodologies.
Assuntos
Liliaceae , Melanthiaceae , Saponinas , Transcriptoma/genética , Perfilação da Expressão Gênica , Liliaceae/genética , Liliaceae/química , Folhas de Planta , Saponinas/genética , Saponinas/análise , Saponinas/química , Melanthiaceae/genética , Melanthiaceae/químicaRESUMO
NRAMP family genes participate in the absorption and transport of heavy metals such as cadmium (Cd), zinc (Zn), copper (Cu), lead (Pb), iron (Fe) and manganese (Mn) and play an important role in the response to heavy metal stress. There is an abundance of research on these genes in bacteria, plants and fungi, although not in S. tuberosum. A total of 48 members(potato(5), Arabidopsis(7), Tomato(9), pepper(9), rice(12) and tobacco(6)) were identified from 6 species (potato (Solanum tuberosum), Arabidopsis thaliana, Tomato (Solanum lycopersicum), pepper (Capsicum annuum), rice (Oryza sativa) and tobacco (Nicotiana attenuate)) and were classified into four subgroups. Across NRAMP gene family members, there are 15 highly conserved motifs that have similar genetic structures and characteristics. In addition, a total of 16 pairs of colinear genes were found in eight species. Analysis of cis-elements indicated that, in response to abiotic stress, NRAMPs are mainly regulated by phytohormones and transcription factors. In addition, analysis of expression profiles indicated that StNRAMP4 is mainly expressed in the roots. According to a qRT-PCR-based analysis of the StNRAMP family, with the exception of Pb2+ stress, StNRAMPs positively responded to stress from Cu2+, Cd2+, Zn2+ and Ni2+ and The expression patterns is similar of StNRAMP2, under Pb2+, and Cu2+ treatment, the relative expression peaked at 24 h. the relative expression peaked at 12 h and was upregulated 428-fold in the roots under Ni2+ stress. Under Cd2+ stress, StNRAMP3 was upregulated 28-fold in the leaves. StNRAMP1, StNRAMP4 and StNRAMP5 showed significant upregulation under Cu2+, Cd2+ and Zn2+ stress, respectively. Expression of StNRAMPs could be specifically induced by heavy metals, implying their possible role in the transport and absorption of heavy metals. This research explains the colinear characteristics of NRAMPs in several food crop species, which is useful for providing important genetic resources for cultivating food crop that accumulate low amounts of heavy metals and for explaining the biological functions of NRAMPs in plants.
Assuntos
Metais Pesados/metabolismo , Proteínas de Plantas/genética , Proteínas de Plantas/metabolismo , Solanum tuberosum/fisiologia , Estresse Fisiológico/genética , Regulação da Expressão Gênica de Plantas , Genoma de Planta/genética , Família Multigênica , Elementos Reguladores de Transcrição , Solanum tuberosum/genética , Solanum tuberosum/metabolismoRESUMO
BACKGROUND: Rosai-Dorfman disease (RDD) is a rare, multisystemic histiocytic disorder, and commonly manifesting as lymphadenopathy in the young male. Abdominal manifestations of RDD are extremely rare. CASE PRESENTATION: In August 2018, a 42-year-old man underwent an abdominal ultrasonography examination due to his weight loss of 10 kg in only three months and found a giant solid tumor was found in his spleen. Then, he was admitted to our hospital and diagnosed as a splenic mass via abdominal enhanced CT and MRI. Laparoscopic splenectomy was administrated within six days of admission due to the clear surgical indications. The pathogenesis of RDD remained poorly understood and the disease should be diagnosed based on histopathology and immunohistochemistry (IHC). The mutations in ATM and NFKBIA were observed using next generation sequencing (NGS). CONCLUSION: We reported a case of splenic involvement of RDD with NGS genetic testing, indicating the difficulty of making a diagnosis before surgery. This extremely rare case offers new references for the understanding of abdominal viscera RDD.
Assuntos
Histiocitose Sinusal , Esplenopatias , Adulto , Histiocitose Sinusal/diagnóstico por imagem , Histiocitose Sinusal/cirurgia , Humanos , Imuno-Histoquímica , Linfadenopatia , Imageamento por Ressonância Magnética , Masculino , Esplenopatias/diagnóstico por imagem , Esplenopatias/cirurgiaRESUMO
The metal tolerance protein (MTP) family is a very old family with evolutionary conservation and less specific amplification. It seems to retain the original functions of the ancestral genes and plays an important role in maintaining metal homeostasis in plant cells. We identified the potato MTP family members for the first time, the specific and conservative StMPTs were discovered by using systematic and comparative omics. To be surprised, members of the StMTP family seem to have mutated before the evolution of dicotyledon and monocotyledon, and even the loss of the entire subfamily (subfamily G6, G7). Interestingly, StMTP9 represents the conserved structure of the entire subfamily involved in toxic metal regulation. However, the gene structure and transmembrane domain of StMTP8 have undergone specific evolution, showing that the transmembrane domain (Motif13) located at the NH2 terminal has been replaced by the signal peptide domain, so it was selected as the control gene of StMTP9. Through real-time fluorescence quantitative analysis of StMTPs under Cd and Zn stress, a co-expression network was constructed, and it was found that StMTP9 responded significantly to Cd stress, while StMTP8 did the opposite. What excites us is that by introducing StMTPs 8/9 into the ∆ycf1 yeast cadmium-sensitive mutant strain, the functional complementation experiment proved that StMTPs 8/9 can restore Cd tolerance. In particular, StMTP9 can greatly reduce the cadmium content in yeast cells, while StMTP8 cannot. These findings provide a reference for further research on the molecular mechanism of potato toxic metal accumulation.
Assuntos
Cádmio/metabolismo , Proteínas de Plantas/metabolismo , Saccharomyces cerevisiae/metabolismo , Solanum tuberosum/genética , Proteínas de Plantas/genética , Proteínas Recombinantes/genética , Proteínas Recombinantes/metabolismo , Saccharomyces cerevisiae/genéticaRESUMO
EP-biochar was produced from Enteromorpha prolifera (EP) at temperatures of 200-600°C under limited-oxygen conditions and then activated using HCl and HF. To optimize the sorption of pyrene (PYR) and benzo[a]pyrene (BaP), the effect of the pyrolysis temperature was studied, and the results showed that EP-biochar produced at 500°C gave the highest removal efficiency. The physiochemical properties of EP-biochar pyrolyzed at 500°C were characterized. The examination indicated that the surface area of EP-biochar was 205.32m2/g. The effect of the EP-biochar dosage and initial solution pH on the adsorption were studied in batch adsorption experiments. Kinetic studies indicated that the adsorption processes of PYR and BaP agreed well with a pseudo second-order kinetic model. The sorption equilibrium data were well described by the Langmuir model. Desorption experiments were conducted to test the strength of binding interactions of EP-biochar. The results showed that PYR and BaP were difficult to dissolve in water after adsorption. Regeneration experiments demonstrated that the biochars regenerated at 200°C retained approximately 48% and 40% of their initial PYR and BaP uptake.
Assuntos
Carvão Vegetal/química , Clorófitas/química , Hidrocarbonetos Policíclicos Aromáticos/análise , Poluentes Químicos da Água/análise , Purificação da Água/métodos , Adsorção , Temperatura Alta , Cinética , Propriedades de SuperfícieRESUMO
Changes in root exudates, including low molecular weight organic acids (LMWOAs), amino acids and sugars, in rhizosphere soils during the gel-beads/reeds combination remediation for high molecular weight polycyclic aromatic hydrocarbons (HMW-PAHs) and the degree of the effects on HMW-PAH biodegradation were evaluated in this study. The results showed that the gel-beads/reeds combination remediation notably increased the removal rates of pyrene, benzo(a)pyrene and indeno(1,2,3-cd)pyrene (65.0-68.9%, 60.0-68.5% and 85.2-85.9%, respectively). During the removal of HMW-PAHs, the LMWOAs, particularly maleic acid, enhanced the biodegradation of HMW-PAHs. Arginine and trehalose monitored in reed root exudates promoted the growth of plants and microorganisms and then improved the removal of HMW-PAHs, especially pyrene. However, the contribution of reed root exudates on degradation of 5- and 6-ring PAHs was minor. These results indicated that the utilization of root exudates was certainly not the only important trait for the removal of HMW-PAHs.
Assuntos
Raízes de Plantas/química , Hidrocarbonetos Policíclicos Aromáticos , Poluentes do Solo , Aminoácidos/química , Recuperação e Remediação Ambiental , Maleatos/química , Peso Molecular , Hidrocarbonetos Policíclicos Aromáticos/química , Hidrocarbonetos Policíclicos Aromáticos/metabolismo , Rizosfera , Poluentes do Solo/química , Poluentes do Solo/metabolismo , Trealose/químicaRESUMO
In this paper, the modified coal cinders and zeolite powders in proportion of 2:1 were mixed with modified polyvinyl alcohol (PVA) with a ratio of 20:1 (w/v) to make a new sorbent and biological carrier-the coal cinder-zeolite balls (CCZBs). The maximum absorption capacities of ammonia nitrogen and Chemical Oxygen Demand (CODCr) on CCZBs, adsorption process were evaluated in batch experiments. And then they were combined with reed wetland for bioremediation of micro-polluted aquaculture water in estuarine wetlands. The results showed that the removal efficiencies of ammonia nitrogen and CODCr improved with the decrease in water inflow and increase in inflow concentrations. Efficiencies of 67.3% and 71.3% for ammonia nitrogen and CODCr under water flow of 10 L/h were obtained when their inflow concentrations were 1.77 and 56.0 mg/L respectively. This strategy can be served as a model system for bioremediation in situ of aquaculture water and other organic polluted or eutrophic water.
Assuntos
Aquicultura/métodos , Purificação da Água/métodos , Áreas Alagadas , Zeolitas , Adsorção , Amônia/química , Biodegradação Ambiental , Análise da Demanda Biológica de Oxigênio , Carvão Mineral , Estuários , Nitrogênio/análise , Nitrogênio/química , Álcool de Polivinil/química , Pós/química , Poluição da Água , Zeolitas/químicaRESUMO
Objective: We isolated an aerobic denitrifier with affinity for nitrogen removing and low accumulation of nitrous oxide, and analyzed its denitrification activity to provide technical support for controlling eutrophic wetland lakes. Methods: An aerobic denitrifier named C3 was isolated from phragmites wetland soil in Liaohe estuary by BTB medium preliminary screening and denitrification activity analysis. We used morphological and physiological characteristics as well as sequence analysis of the 16S rRNA to identify the strain. Effects of culture conditions, such as temperature, carbon source, initial pH, C/N ratio were evaluated regarding removal of nitrite nitrogen and accumulation of nitrous oxide under aerobic condition. Results: The highly effective strain C3 was identified as Pseudomonas sp., for which the best carbon source was sodium citrate, optimal growth and denitrification occurred at 30â, pH 7.0, and a C/N ratio of 10. Under these conditions, the concentration of nitrate was reduced from 179.55 mg/L to 5.08 mg/L by strain C3 in 36 hours, a reduction in NO3- of more than 97.17%. In the process of denitrification, nitrous oxide as an intermediate accumulated at a level of 0.22 mg/L. Conclusion: Strain C3, an aerobic denitrifying agent, was isolated from wetland soil and has obvious advantages in efficient removal of nutrients with low accumulation of nitrous oxide, representing a great potential to treat eutrophic wetland lakes.
Assuntos
Nitratos/metabolismo , Nitritos/metabolismo , Pseudomonas/isolamento & purificação , Pseudomonas/metabolismo , Aerobiose , Carbono/metabolismo , China , Desnitrificação , Estuários , Filogenia , Pseudomonas/classificação , Pseudomonas/genética , Solo/química , Microbiologia do Solo , Áreas AlagadasRESUMO
BACKGROUND: Cold ischemia-reperfusion injury (IRI) is a major cause of graft failure in lung transplantation. Despite therapeutic benefits of mesenchymal stem cells (MSCs) in attenuating acute lung injury, their protection of lung transplants from cold IRI remains elusive. The present study was to test the efficacy of MSCs in the prevention of cold IRI using a novel murine model of orthotopic lung transplantation. METHODS: Donor lungs from C57BL/6 mice were exposed to 6 h of cold ischemia before transplanted to syngeneic recipients. MSCs were isolated from the bone marrows of C57BL/6 mice for recipient treatment. Gas exchange was determined by the measurement of blood oxygenation, and lung injury and inflammation were assessed by histological analyses. RESULTS: Intravenously delivered MSC migration/trafficking to the lung grafts occurred within 4-hours post-transplantation. As compared to untreated controls, the graft arterial blood oxygenation (PaO2/FiO2) capacity was significantly improved in MSC-treated recipients as early as 4 h post-reperfusion and such improvement continued over time. By 72 h, oxygenation reached normal level that was not seen in controls. MSCs treatment conferred significant protection of the grafts from cold IRI and cell apoptosis, which is correlated with less cellular infiltration, a decrease in proinflammatory cytokines (TNF-α, IL-6) and toll-like receptor 4, and an increase in anti-inflammatory TSG-6 generation. CONCLUSIONS: MSCs provide significant protection against cold IRI in lung transplants, and thus may be a promising strategy to improve outcomes after lung transplantation.
Assuntos
Isquemia Fria/efeitos adversos , Transplante de Pulmão/efeitos adversos , Pulmão/cirurgia , Transplante de Células-Tronco Mesenquimais , Células-Tronco Mesenquimais , Traumatismo por Reperfusão/prevenção & controle , Animais , Apoptose , Moléculas de Adesão Celular/metabolismo , Células Cultivadas , Modelos Animais de Doenças , Proteínas de Fluorescência Verde/biossíntese , Proteínas de Fluorescência Verde/genética , Mediadores da Inflamação/metabolismo , Infusões Intravenosas , Interleucina-6/metabolismo , Pulmão/metabolismo , Pulmão/patologia , Masculino , Células-Tronco Mesenquimais/metabolismo , Camundongos Endogâmicos C57BL , Camundongos Transgênicos , Pneumonia/etiologia , Pneumonia/prevenção & controle , Traumatismo por Reperfusão/etiologia , Traumatismo por Reperfusão/metabolismo , Traumatismo por Reperfusão/patologia , Fatores de Tempo , Receptor 4 Toll-Like/metabolismo , Fator de Necrose Tumoral alfa/metabolismoRESUMO
PURPOSE: Cardiac allograft vasculopathy (CAV) is a major complication limiting the long-term survival of cardiac transplants. The role of memory T cells (Tmem) in the pathogenesis of CAV remains elusive. This study investigated the role of Tmem cells in the development of CAV and the therapeutic potential of targeting the OX40/OX40L pathway for heart transplant survival. METHODS: Tmem cells were generated in Rag-1(-/-) C57BL/6 (B6) mice by homeostatic proliferation (HP) of CD40L null CD3(+) T cells from B6 mice. Rag-1(-/-) B6 mice (H-2(b)) harboring Tmem cells received cardiac allografts from BALB/c mice (H-2(d)), and were either untreated or treated with anti-OX40L monoclonal antibody (mAb) (0.5 mg/mouse/day) for 10 days. RESULTS: Six weeks after HP, the majority of transferred CD40L(-/-) T cells in Rag-1(-/-) B6 mice were differentiated to CD44(high) and CD62L(low) Tmem cells. BALB/c heart allografts in Rag-1(-/-) B6 recipient mice in the presence of these Tmem cells developed a typical pathological feature of CAV; intimal thickening, 100 days after transplantation. However, functionally blocking the OX40/OX40L pathway with anti-OX40L mAb significantly prevented CAV development and reduced the Tmem cell population in recipient mice. Anti-OX40L mAb therapy also significantly decreased cellular infiltration and cytokine (IFN-γ, TNF-α and TGF-ß) expression in heart allografts. CONCLUSIONS: Tmem cells mediate CAV in heart transplants. Functionally blocking the OX40/OX40L pathway using anti-OX40L mAb therapy prevents Tmem cell-mediated CAV, suggesting therapeutic potential for disrupting OX40-OX40L signaling in order to prevent CAV in heart transplant patients.
Assuntos
Anticorpos Monoclonais/imunologia , Transplante de Coração/efeitos adversos , Memória Imunológica/imunologia , Glicoproteínas de Membrana/imunologia , Linfócitos T/imunologia , Fatores de Necrose Tumoral/imunologia , Aloenxertos , Animais , Receptores de Hialuronatos/imunologia , Interferon gama/imunologia , Selectina L/imunologia , Masculino , Camundongos , Camundongos Endogâmicos BALB C , Camundongos Endogâmicos C57BL , Ligante OX40 , Receptores OX40/imunologia , Fator de Crescimento Transformador beta/imunologia , Fator de Necrose Tumoral alfa/imunologiaRESUMO
In recent years, legged robots have been more and more widely used on non-structured terrain, and their foot structure has an important impact on the robot's motion performance and stability. The structural characteristics of the yak foot sole with a high outer edge and low middle, which has excellent soil fixation ability and is an excellent bionic prototype, can improve the friction between the foot and the ground. At the same time, the foot hooves can effectively alleviate the larger impact load when contacting with the ground, which is an excellent anti-slip buffer mechanism. The bionic foot end design was carried out based on the morphology of the yak sole; the bionic foot design was carried out based on the biological anatomy observation of yak foot skeletal muscles. The virtual models of the bionic foot end and the bionic foot were established and simulated using Solidworks 2022 and Abaqus 2023, and the anti-slip performance on different ground surfaces and the influence of each parameter of the bionic foot on the cushioning effect were investigated. The results show that (1) the curved shape of the yak sole has a good anti-slip performance on both soil ground and rocky ground, and the anti-slip performance is better on soil ground; (2) the curved shape of the yak sole has a larger maximum static friction than the traditional foot, and the anti-slip performance is stronger under the same pressure conditions; (3) the finger pillow-hoof ball structure of the bionic foot has the greatest influence on the buffering effect, and the buffering effect of the bionic foot is best when the tip of the bionic foot touches the ground first.
RESUMO
The accumulation of agricultural chemicals in the environment has become a global concern, of which sulfonylurea herbicides (SUHs) constitute a significant category. Solar-driven photocatalysis is favored for removing organic pollutants due to its high efficiency and environmental friendliness. Graphite carbon nitride (g-C3N4)-based materials with superior catalytic activities and physicochemical stabilities are promising photocatalysts. This review describes the g-C3N4-based materials and their uses in the photocatalytic degradation of SUHs or other organic pollutants with similar structures. First, the fundamentals of g-C3N4-based materials and photocatalytic SUHs degradation are discussed to provide an in-depth understanding of the mechanism for the photocatalytic activity. The ability of different g-C3N4-based materials to photocatalytically degrade SUH-like structures is then discussed and summarized based on different modification strategies (morphology modulation, elemental doping, defect engineering, and heterojunction formations). Meanwhile, the effects of different environmental factors on the photocatalytic performance of g-C3N4-based materials are described. Finally, the major challenges and opportunities of g-C3N4-based materials for the photocatalytic degradation of SUHs are proposed. It is hoped that this review will show the feasibility of photocatalytic degradation of SUHs with g-C3N4-based materials.
Assuntos
Poluentes Ambientais , CatáliseRESUMO
Estuaries and coasts are located at the land-sea interface, where sediment liquefaction due to strong wave action results in significant material exchange at the sediment-seawater system. Polycyclic aromatic hydrocarbons (PAHs), as organic pollutants, are distributed across various media. Herein, the impact of wave was studied on the release of PAHs through indoor microcosmic experiments combined with a level IV fugacity model. Comparison revealed that the release amount and rate of PAHs during static consolidation stage were minimal, whereas wave action substantially enhanced the release. Particularly the sediments in a liquefied state, the PAHs release in Stage III was 1.55-1.86 times that in Stage II, reaching 84.73 µg/L. The loss of soil strength and strong hydrodynamic effects resulted in a substantial release of PAHs into seawater along with suspended solids. Due to volatility of 2-ring PAHs and difficult desorption of 6-ring PAHs, 3-5-ring PAHs are the main contributors to releases into seawater. The model results also indicated that the three PAHs had different fates in the sediment-seawater system, with sediment serving as an important "reservoir" for benzo[a]pyrene entering seawater, while functioning as both a "sink" and a "source" for pyrene.
RESUMO
The effects of two benthonic species, Perinereis aibuhitensis and Matuta planipes Fabricius, on the release of polycyclic aromatic hydrocarbons (PAHs) from sediments were investigated using a sediment-seawater microcosm. A Level IV fugacity model was used to simulate the behavior and fate of PAHs in the environment. This study revealed that both benthos significantly influenced the release of PAHs, and Matuta planipes Fabricius had a stronger disturbance effect than another. The final concentrations of Matuta planipes Fabricius group, Perinereis aibuhitensis group and the control group in the seawater phase reached 10.8, 9.94 and 7.90 µg/L, respectively. There were certain differences in the behaviour of the two benthonic species. Matuta planipes Fabricius caused more sediment resuspension, while Perinereis aibuhitensis increased the total organic carbon (TOC) content in the environment. The vertical concentration distribution of sediment indicated that vertical mixing was slightly stronger in the Matuta planipes Fabricius group than that in the Perinereis aibuhitensis group. The fugacity model effectively simulated the release behavior of PAHs, providing insight into PAH transport and distribution. The results demonstrated that bioturbation could promote the release of PAHs from seawater. The amount of PAHs released was significantly correlated with the biological habits of the benthos.
Assuntos
Sedimentos Geológicos , Hidrocarbonetos Policíclicos Aromáticos , Água do Mar , Poluentes Químicos da Água , Hidrocarbonetos Policíclicos Aromáticos/análise , Hidrocarbonetos Policíclicos Aromáticos/química , Sedimentos Geológicos/química , Poluentes Químicos da Água/análise , Poluentes Químicos da Água/química , Água do Mar/química , Monitoramento Ambiental , AnimaisRESUMO
Sustainability assessments of coastal beach exploitation are difficult because the identification of appropriate monitoring methodologies and evaluation procedures is still ongoing. In particular, the most suitable procedure for the application of sustainability assessment to coastal beaches remains uncertain. This paper presents a complete sustainability assessment process for coastal beach exploitation based on the analytic hierarchy process (AHP). We developed an assessment framework consisting of 14 indicators derived from the three dimensions of suitability, economic and social value, and ecosystem. We chose a wind power project on a coastal beach of Yancheng as a case study. The results indicated that the wind power farms on the coastal beach were not completely in keeping with sustainable development theory. The construction of the wind power farms had some negative impacts. Therefore, in the design stage, wind turbines should be designed and planned carefully to minimize these negative impacts. In addition, the case study demonstrated that the AHP was capable of addressing the complexities associated with the sustainability of coastal beaches.