RESUMO
Central nervous system myelin is a specialized structure produced by oligodendrocytes that ensheaths axons, allowing rapid and efficient saltatory conduction of action potentials. Many disorders promote damage to and eventual loss of the myelin sheath, which often results in significant neurological morbidity. However, little is known about the fundamental mechanisms that initiate myelin damage, with the assumption being that its fate follows that of the parent oligodendrocyte. Here we show that NMDA (N-methyl-d-aspartate) glutamate receptors mediate Ca2+ accumulation in central myelin in response to chemical ischaemia in vitro. Using two-photon microscopy, we imaged fluorescence of the Ca2+ indicator X-rhod-1 loaded into oligodendrocytes and the cytoplasmic compartment of the myelin sheath in adult rat optic nerves. The AMPA (alpha-amino-3-hydroxy-5-methyl-4-isoxazole propionic acid)/kainate receptor antagonist NBQX completely blocked the ischaemic Ca2+ increase in oligodendroglial cell bodies, but only modestly reduced the Ca2+ increase in myelin. In contrast, the Ca2+ increase in myelin was abolished by broad-spectrum NMDA receptor antagonists (MK-801, 7-chlorokynurenic acid, d-AP5), but not by more selective blockers of NR2A and NR2B subunit-containing receptors (NVP-AAM077 and ifenprodil). In vitro ischaemia causes ultrastructural damage to both axon cylinders and myelin. NMDA receptor antagonism greatly reduced the damage to myelin. NR1, NR2 and NR3 subunits were detected in myelin by immunohistochemistry and immunoprecipitation, indicating that all necessary subunits are present for the formation of functional NMDA receptors. Our data show that the mature myelin sheath can respond independently to injurious stimuli. Given that axons are known to release glutamate, our finding that the Ca2+ increase was mediated in large part by activation of myelinic NMDA receptors suggests a new mechanism of axo-myelinic signalling. Such a mechanism may represent a potentially important therapeutic target in disorders in which demyelination is a prominent feature, such as multiple sclerosis, neurotrauma, infections (for example, HIV encephalomyelopathy) and aspects of ischaemic brain injury.
Assuntos
Cálcio/metabolismo , Isquemia/metabolismo , Isquemia/patologia , Bainha de Mielina/metabolismo , Receptores de N-Metil-D-Aspartato/metabolismo , Animais , Sinalização do Cálcio/efeitos dos fármacos , Antagonistas de Aminoácidos Excitatórios/farmacologia , Bainha de Mielina/efeitos dos fármacos , Oligodendroglia/efeitos dos fármacos , Oligodendroglia/metabolismo , Nervo Óptico/citologia , Nervo Óptico/patologia , Ratos , Ratos Long-Evans , Receptores de Glutamato/metabolismo , Receptores de N-Metil-D-Aspartato/antagonistas & inibidoresRESUMO
Ultrastructural studies have shown that during early stages of Schwann cell myelination mesaxon membranes are converted to compact myelin lamellae. The distinct changes that occur in the spacing of these Schwann cell membranes are likely to be mediated by the redistribution of (a) the myelin-associated glycoprotein, a major structural protein of mesaxon membranes; and (b) P0 protein, the major structural protein of compact myelin. To test this hypothesis, the immunocytochemical distribution of these two proteins was determined in serial 1-micron-thick Epon sections of ventral roots from quaking mice and compared to the ultrastructure of identical areas in an adjacent thin section. Ventral roots of this hypomyelinating mouse mutant were studied because many fibers have a deficit in converting mesaxon membranes to compact myelin. The results indicated that conversion of mesaxon membranes to compact myelin involves the insertion of P0 protein into and the removal of the myelin-associated glycoprotein from mesaxon membranes. The failure of some quaking mouse Schwann cells to form compact myelin appears to result from an inability to remove the myelin-associated glycoprotein from their mesaxon membranes.
Assuntos
Glicoproteínas de Membrana/análise , Proteínas da Mielina/análise , Bainha de Mielina/fisiologia , Nervos Periféricos/análise , Animais , Axônios/análise , Axônios/ultraestrutura , Membrana Celular/análise , Membrana Celular/fisiologia , Imuno-Histoquímica , Camundongos , Camundongos Quaking , Microscopia Eletrônica , Proteína P0 da Mielina , Bainha de Mielina/análise , Bainha de Mielina/ultraestrutura , Glicoproteína Associada a Mielina , Nervos Periféricos/ultraestrutura , Células de Schwann/fisiologia , Células de Schwann/ultraestruturaRESUMO
The myelin-associated glycoprotein (MAG) is an integral membrane protein (congruent to 100,000 mol wt) which is a minor component of purified peripheral nervus system (PNS) myelin. In the present study, MAG was localized immunocytochemically in 1-micrometer thick Epon sections of 7-d and adult rat peripheral nerves, and its localization was compared to that of the major structural protein (Po) of PNS myelin. To determine more precisely the localization of MAG, immunostained areas in 1 micrometer sections were traced on electron micrographs of identical areas from adjacently cut thin sections.l MAG was localized in periaxonal membranes. Schmidt-Lantermann incisures, paranodal membranes, and the outer mesaxon of PNS myelin sheaths. Compact regions of PNS myelin did not react with MAG antiserum. The results demonstrate MAG's presence in "'semi-compact" Schwann cell or myelin membranes that have a gap of 12-14 nm between extracellular leaflets and a spacing of 5 nm or more between cytoplasmic leaflets. In compact regions of the myelin sheath which do not contain MAG, the cytoplasmic leaflets are "fused" and form the major dense line, whereas the extracellular leaflets are separated by a 2.0 nm gap appearing as paired minor dense lines. Thus, it is proposed that MAG plays a role in maintaining the periaxonal space, Schmidt-Lantermann incisures, paranodal myelin loops, and outer mesaxon by preventing "complete" compaction of Schwann cell and myelin membranes. The presence of MAG in these locations also suggests that MAG may serve a function in regulating myelination in the PNS.
Assuntos
Proteínas da Mielina/análise , Bainha de Mielina/análise , Animais , Citoplasma/análise , Técnicas Imunoenzimáticas , Proteína P0 da Mielina , Bainha de Mielina/ultraestrutura , Fibras Nervosas Mielinizadas/análise , Ratos , Ratos Endogâmicos , Células de Schwann/análise , Células de Schwann/ultraestruturaRESUMO
The myelin-associated glycoprotein (MAG) is an integral membrane glycoprotein that is located in the periaxonal membrane of myelin-forming Schwann cells. On the basis of this localization, it has been hypothesized that MAG plays a structural role in (a) forming and maintaining contact between myelinating Schwann cells and the axon (the 12-14-nm periaxonal space) and (b) maintaining the Schwann cell periaxonal cytoplasmic collar of myelinated fibers. To test this hypothesis, we have determined the immunocytochemical localization of MAG in the L4 ventral roots from 11-mo-old quaking mice. These roots display various stages in the association of remyelinating Schwann cells with axons, and abnormalities including loss of the Schwann cell periaxonal cytoplasmic collar and dilation of the periaxonal space of myelinated fibers. Therefore, this mutant provides distinct opportunities to observe the relationships between MAG and (a) the formation of the periaxonal space during remyelination and (b) the maintenance of the periaxonal space and Schwann cell periaxonal cytoplasmic collar in myelinated fibers. During association of remyelinating Schwann cells and axons, MAG was detected in Schwann cell adaxonal membranes that apposed the axolemma by 12-14 nm. Schwann cell plasma membranes separated from the axolemma by distances greater than 12-14 nm did not react with MAG antiserum. MAG was present in adaxonal Schwann cell membranes that apposed the axolemma by 12-14 nm but only partially surrounded the axon and, therefore, may be actively involved in the ensheathment of axons by remyelinating Schwann cells. To test the dual role of MAG in maintaining the periaxonal space and Schwann cell periaxonal cytoplasmic collar of myelinated fibers, we determined the immunocytochemical localization of MAG in myelinated quaking fibers that displayed pathological alterations of these structures. Where Schwann cell periaxonal membranes were not stained by MAG antiserum, the cytoplasmic side of the periaxonal membrane was "fused" with the cytoplasmic side of the inner compact myelin lamella and formed a major dense line. This loss of MAG and the Schwann cell periaxonal cytoplasmic collar usually resulted in enlargement of the 12-14-nm periaxonal space and ruffling of the apposing axolemma. In myelinated fibers, there was a strict correlation between the presence of MAG in the Schwann cell periaxonal membrane and (a) maintenance of the 12-14-nm periaxonal space, and (b) presence of the Schwann cell periaxonal cytoplasmic collar.(ABSTRACT TRUNCATED AT 400 WORDS)
Assuntos
Axônios/ultraestrutura , Camundongos Quaking/fisiologia , Proteínas da Mielina/fisiologia , Bainha de Mielina/ultraestrutura , Células de Schwann/ultraestrutura , Animais , Técnicas Imunoenzimáticas , Camundongos , Microscopia Eletrônica , Glicoproteína Associada a Mielina , Células de Schwann/citologia , Medula Espinal/citologia , Medula Espinal/ultraestruturaRESUMO
The myelin-associated glycoprotein (MAG) is a member of the immunoglobulin gene superfamily that is selectively expressed by myelin-forming cells. A developmentally regulated, alternative splicing of a single MAG transcript produces two MAG polypeptides (72 and 67 kD) in the central nervous system (CNS). MAG occurs predominantly as the 67-kD polypeptide in the peripheral nervous system (PNS). This study determined the subcellular localization of CNS MAG at different postnatal times when the 72-kD form (7-d) and 67-kD form (adult) are quantitatively abundant. These distributions were also compared to those of MAG in the PNS. In adult rat, MAG is selectively enriched in periaxonal membranes of CNS myelin internodes. This restricted distribution differs from that in PNS myelin internodes where MAG is also enriched in paranodal loops, Schmidt-Lanterman incisures, and mesaxon membranes. In 7-d-old rat CNS, MAG was associated with periaxonal membranes during axonal ensheathment and enriched in Golgi membranes and cytoplasmic organelles having the appearance of multivesicular bodies (MVBs). MAG-enriched MVBs were found in oligodendrocyte perinuclear regions, in processes extending to myelin internodes, and along the myelin internode in outer tongue processes and paranodal loops. MAG-enriched MVBs were not found in oligodendrocytes from adult animals or in myelinating Schwann cells. These findings raise the possibility that the 72-kD MAG polypeptide is associated with receptor-mediated endocytosis of components from the periaxonal space or axolemma during active stages of myelination.
Assuntos
Axônios/ultraestrutura , Proteínas da Mielina/análise , Oligodendroglia/ultraestrutura , Nervo Isquiático/ultraestrutura , Medula Espinal/ultraestrutura , Envelhecimento , Animais , Genes de Imunoglobulinas , Imuno-Histoquímica , Microscopia Eletrônica , Família Multigênica , Proteínas da Mielina/genética , Bainha de Mielina/ultraestrutura , Glicoproteína Associada a Mielina , Organelas/ultraestrutura , Ratos , Ratos Endogâmicos , Nervo Isquiático/crescimento & desenvolvimento , Medula Espinal/crescimento & desenvolvimentoRESUMO
Previous studies have indicated that newly formed oligodendrocytes are dynamic cells whose production, survival, and differentiation depend upon axonal influences. This study has characterized the appearance and fate of newly formed oligodendrocytes in developing rat brain. Oligodendrocytes appear in predictable locations and radially extend DM-20-positive processes that cover 80-microm domains in the cortex and 40-microm domains in the corpus callosum. These premyelinating oligodendrocytes have one of two fates: they myelinate axons or degenerate. Between 7 and 21 d after birth, approximately 20% of premyelinating oligodendrocytes identified in the cerebral cortex were degenerating. Oligodendrocytes that ensheathed axons expressed and selectively targeted proteolipid protein to compact myelin and did not degenerate. These observations support the hypothesis that axonal influences affect oligodendrocyte survival, differentiation, and expression of proteolipid protein gene products.
Assuntos
Encéfalo/citologia , Proteína Proteolipídica de Mielina/análise , Proteínas do Tecido Nervoso , Oligodendroglia/citologia , Animais , Axônios/metabolismo , Encéfalo/crescimento & desenvolvimento , Morte Celular , Diferenciação Celular , Córtex Cerebral/química , Córtex Cerebral/citologia , Cromatina/química , Corpo Caloso/química , Corpo Caloso/citologia , Oligodendroglia/metabolismo , Ratos , Ratos Sprague-DawleyRESUMO
The myelin-associated glycoprotein (MAG) is a heavily glycosylated integral membrane glycoprotein which is a minor component of isolated rat peripheral nervous system (PNS) myelin. Immunocytochemically MAG has been localized in the periaxonal region of PNS myelin sheaths. The periaxonal localization and biochemical features of MAG are consistent with the hypothesis that MAG plays a role in maintaining the periaxonal space of myelinated fibers. To test this hypothesis, MAG was localized immunocytochemically in 1-micron sections of the L5 ventral root from rats exposed to B,B'-iminodipropionitrile. In chronic states of B,B'-iminodipropionitrile intoxication, Schwann cell periaxonal membranes and the axolemma invaginate into giant axonal swellings and separate a central zone of normally oriented axoplasm from an outer zone of maloriented neurofilaments. Ultrastructurally, the width of the periaxonal space (12-14 nm) in the ingrowths is identical to that found in normally myelinated fibers. These Schwann cell ingrowths which are separated from compact myelin by several micra are stained intensely by MAG antiserum. Antiserum directed against Po protein, the major structural protein of compact PNS myelin, does not stain the ingrowths unless compact myelin is present. These results demonstrate the periaxonal localization of MAG and support a functional role for MAG in maintaining the periaxonal space of PNS myelinated fibers.
Assuntos
Axônios/fisiologia , Proteínas da Mielina/análise , Bainha de Mielina/fisiologia , Doenças do Sistema Nervoso/patologia , Células de Schwann/fisiologia , Animais , Axônios/ultraestrutura , Microscopia Eletrônica , Bainha de Mielina/ultraestrutura , Glicoproteína Associada a Mielina , Doenças do Sistema Nervoso/induzido quimicamente , Nitrilas , Ratos , Ratos Endogâmicos , Células de Schwann/ultraestruturaRESUMO
Membrane-membrane interactions between axons and Schwann cells are required for initial myelin formation in the peripheral nervous system. However, recent studies of double myelination in sympathetic nerve have indicated that myelin sheaths continue to exist after complete loss of axonal contact (Kidd, G. J., and J. W. Heath. 1988. J. Neurocytol. 17:245-261). This suggests that myelin maintenance may be regulated either by diffusible axonal factors or by nonaxonal mechanisms. To test these hypotheses, axons involved in double myelination in the rat superior cervical ganglion were destroyed by chronic guanethidine treatment. Guanethidine-induced sympathectomy resulted in a Wallerian-like pattern of myelin degeneration within 10 d. In doubly myelinated configurations the axon, inner myelin sheath (which lies in contact with the axon), and approximately 75% of outer myelin sheaths broke down by this time. Degenerating outer sheaths were not found at later periods. It is probably that outer sheaths that degenerated were only partially displaced from the axon at the commencement of guanethidine treatment. In contrast, analysis of serial sections showed that completely displaced outer internodes remained ultrastructurally intact. These internodes survived degeneration of the axon and inner sheath, and during the later time points (2-6 wk) they enclosed only connective tissue elements and reorganized Schwann cells/processes. Axonal regeneration was not observed within surviving outer internodes. We therefore conclude that myelin maintenance in the superior cervical ganglion is not dependent on direct axonal contact or diffusible axonal factors. In addition, physical association of Schwann cells with the degenerating axon may be an important factor in precipitating myelin breakdown during Wallerian degeneration.
Assuntos
Guanetidina/farmacologia , Bainha de Mielina/ultraestrutura , Degeneração Neural/efeitos dos fármacos , Animais , Axônios/efeitos dos fármacos , Axônios/ultraestrutura , Gânglios Simpáticos/efeitos dos fármacos , Gânglios Simpáticos/ultraestrutura , Masculino , Microscopia Eletrônica , Bainha de Mielina/efeitos dos fármacos , Ratos , Ratos Endogâmicos , Células de Schwann/efeitos dos fármacos , Células de Schwann/ultraestrutura , Fatores de TempoRESUMO
Quaking is an autosomal recessive hypo/dysmyelinating mutant mouse which has a 1-Mbp deletion on chromosome 17. The mutation exhibits pleiotrophy and does not include genes encoding characterized myelin proteins. The levels of the 67-kD isoform of the myelin-associated glycoprotein (S-MAG) relative to those of the 72-kD isoform (L-MAG) are increased in the quaking CNS, but not in other dysmyelinating mutants. Abnormal expression of MAG isoforms in quaking may result from altered transcription of the MAG gene or from abnormal sorting, transport, or targeting of L-MAG or S-MAG. To test these hypotheses, we have determined the distribution of L-MAG and S-MAG in cervical spinal cord of 7-, 14-, 21-, 28-, and 35-d-old quaking mice. In 7-d-old quaking and control spinal cord, L- and S-MAG was detectable in periaxonal regions of myelinated fibers and in the perinuclear cytoplasm of oligodendrocytes. Between 7 and 35 d, L-MAG was removed from the periaxonal membrane of quaking but not control mice. Compared to control mice, a significant increase in MAG labeling of endosomes occurred within oligodendrocyte cytoplasm of 35-d-old quaking mice. S-MAG remained in periaxonal membranes of both quaking and control mice. Analysis of the cytoplasmic domain of L-MAG identifies amino acid motifs at tyrosine 35 and tyrosine 65 which meet the criteria for "tyrosine internalization signals" that direct transmembrane glycoproteins into the endocytic pathway. These results establish that L-MAG is selectively removed from the periaxonal membrane of CNS-myelinated fibers by receptor-mediated endocytosis. The loss of L-MAG from quaking periaxonal membranes results from increased endocytosis of L-MAG and possibly a decrease in L-MAG production.
Assuntos
Endocitose/fisiologia , Camundongos Quaking/fisiologia , Bainha de Mielina/química , Glicoproteína Associada a Mielina/análise , Sequência de Aminoácidos , Animais , Especificidade de Anticorpos , Axônios/química , Axônios/ultraestrutura , Imuno-Histoquímica , Isomerismo , Camundongos , Microscopia Confocal , Dados de Sequência Molecular , Proteína Proteolipídica de Mielina/análise , Proteína Proteolipídica de Mielina/imunologia , Glicoproteína Associada a Mielina/imunologia , Glicoproteína Associada a Mielina/metabolismo , Medula Espinal/química , Frações Subcelulares/químicaRESUMO
Retinal ganglion cell axons and axonal electrical activity have been considered essential for migration, proliferation, and survival of oligodendrocyte lineage cells in the optic nerve. To define axonal requirements during oligodendrogenesis, the developmental appearance of oligodendrocyte progenitors and oligodendrocytes were compared between normal and transected optic nerves. In the absence of viable axons, oligodendrocyte precursors migrated along the length of the nerve and subsequently multiplied and differentiated into myelin basic protein-positive oligodendrocytes at similar densities and with similar temporal and spatial patterns as in control nerves. Since transected optic nerves failed to grow radially, the number of oligodendrocyte lineage cells was reduced compared with control nerves. However, the mitotic indices of progenitors and the percentage of oligodendrocytes undergoing programmed cell death were similar in control and transected optic nerves. Oligodendrocytes lacked their normal longitudinal orientation, developed fewer, shorter processes, and failed to form myelin in the transected nerves. These data indicate that normal densities of oligodendrocytes can develop in the absence of viable retinal ganglion axons, and support the possibility that axons assure their own myelination by regulating the number of myelin internodes formed by individual oligodendrocytes.
Assuntos
Axônios/metabolismo , Oligodendroglia/citologia , Nervo Óptico/citologia , Células Ganglionares da Retina/citologia , Células-Tronco/citologia , Animais , Animais Recém-Nascidos , Apoptose , Axônios/ultraestrutura , Diferenciação Celular , Divisão Celular , Linhagem da Célula , Movimento Celular , Núcleo Celular/fisiologia , Tamanho Celular , Sobrevivência Celular , Denervação , Índice Mitótico , Bainha de Mielina/metabolismo , Oligodendroglia/metabolismo , Nervo Óptico/crescimento & desenvolvimento , Nervo Óptico/ultraestrutura , Ratos , Ratos Sprague-Dawley , Retina/citologia , Células Ganglionares da Retina/ultraestrutura , Células-Tronco/metabolismo , Fatores de TempoRESUMO
P0 protein, the dominant protein in peripheral nervous system myelin, was studied immunocytochemically in both developing and mature Schwann cells. Trigeminal and sciatic nerves from newborn, 7-d, and adult rats were processed for transmission electron microscopy. Alternating 1-micrometer-thick Epon sections were stained with paraphenylenediamine (PD) or with P0 antiserum according to the peroxidase-antiperoxidase method. To localize P0 in Schwann cell cytoplasm and myelin membranes, the distribution of immunostaining observed in 1-micrometer sections was mapped on electron micrographs of identical areas found in adjacent thin sections. The first P0 staining was observed around axons and/or in cytoplasm of Schwann cells that had established a 1:1 relationship with axons. In newborn nerves, staining of newly formed myelin sheaths was detected more readily with P0 antiserum than with PD. Myelin sheaths with as few as three lamellae could be identified with the light microscope. Very thin sheaths often stained less intensely and part of their circumference frequently was unstained. Schmidt-Lanterman clefts found in more mature sheaths also were unstained. As myelination progressed, intensely stained myelin rings became much more numerous and, in adult nerves, all sheaths were intensely and uniformly stained. Particulate P0 staining also was observed in juxtanuclear areas of Schwann cell cytoplasm. It was most prominent during development, then decreased, but still was detected in adult nerves. The cytoplasmic areas stained by P0 antiserum were rich in Golgi complex membranes.
Assuntos
Complexo de Golgi/análise , Proteínas da Mielina/análise , Bainha de Mielina/análise , Células de Schwann/análise , Animais , Axônios/ultraestrutura , Citoplasma/análise , Técnicas Imunoenzimáticas , Mitocôndrias/análise , Proteína P0 da Mielina , Ratos , Células de Schwann/fisiologia , Células de Schwann/ultraestruturaRESUMO
This report investigated mechanisms responsible for failed Schwann cell myelination in mice that overexpress P(0) (P(0)(tg)), the major structural protein of PNS myelin. Quantitative ultrastructural immunocytochemistry established that P(0) protein was mistargeted to abaxonal, periaxonal, and mesaxon membranes in P(0)(tg) Schwann cells with arrested myelination. The extracellular leaflets of P(0)-containing mesaxon membranes were closely apposed with periodicities of compact myelin. The myelin-associated glycoprotein was appropriately sorted in the Golgi apparatus and targeted to periaxonal membranes. In adult mice, occasional Schwann cells myelinated axons possibly with the aid of endocytic removal of mistargeted P(0). These results indicate that P(0) gene multiplication causes P(0) mistargeting to mesaxon membranes, and through obligate P(0) homophilic adhesion, renders these dynamic membranes inert and halts myelination.
Assuntos
Proteína P0 da Mielina/metabolismo , Bainha de Mielina/metabolismo , Células de Schwann/metabolismo , Envelhecimento , Animais , Axônios/metabolismo , Axônios/ultraestrutura , Membrana Celular/metabolismo , Membrana Celular/ultraestrutura , Endossomos/metabolismo , Endossomos/ultraestrutura , Amplificação de Genes , Complexo de Golgi/metabolismo , Complexo de Golgi/ultraestrutura , Imuno-Histoquímica , Camundongos , Camundongos Transgênicos , Microscopia Eletrônica , Proteína P0 da Mielina/genética , Bainha de Mielina/ultraestrutura , Glicoproteína Associada a Mielina/metabolismo , RNA Mensageiro/biossíntese , Células de Schwann/citologia , Células de Schwann/ultraestrutura , Nervo Isquiático/metabolismo , Nervo Isquiático/ultraestrutura , Raízes Nervosas Espinhais/metabolismo , Raízes Nervosas Espinhais/ultraestruturaRESUMO
This study investigated the function of the adhesion molecule L1 in unmyelinated fibers of the peripheral nervous system (PNS) by analysis of L1- deficient mice. We demonstrate that L1 is present on axons and Schwann cells of sensory unmyelinated fibers, but only on Schwann cells of sympathetic unmyelinated fibers. In L1-deficient sensory nerves, Schwann cells formed but failed to retain normal axonal ensheathment. L1-deficient mice had reduced sensory function and loss of unmyelinated axons, while sympathetic unmyelinated axons appeared normal. In nerve transplant studies, loss of axonal-L1, but not Schwann cell-L1, reproduced the L1-deficient phenotype. These data establish that heterophilic axonal-L1 interactions mediate adhesion between unmyelinated sensory axons and Schwann cells, stabilize the polarization of Schwann cell surface membranes, and mediate a trophic effect that assures axonal survival.
Assuntos
Axônios/metabolismo , Glicoproteínas de Membrana/metabolismo , Bainha de Mielina/metabolismo , Moléculas de Adesão de Célula Nervosa/metabolismo , Neurônios Aferentes/citologia , Células de Schwann/citologia , Animais , Axônios/ultraestrutura , Adesão Celular , Polaridade Celular , Sobrevivência Celular , Feminino , Gânglios Espinais/citologia , Gânglios Espinais/metabolismo , Deleção de Genes , Complexo Antígeno L1 Leucocitário , Masculino , Glicoproteínas de Membrana/genética , Camundongos , Camundongos Knockout , Bainha de Mielina/ultraestrutura , Glicoproteína Associada a Mielina/genética , Glicoproteína Associada a Mielina/metabolismo , Degeneração Neural , Fibras Nervosas/metabolismo , Fibras Nervosas/ultraestrutura , Moléculas de Adesão de Célula Nervosa/genética , Neurônios Aferentes/metabolismo , Neurônios Aferentes/ultraestrutura , Sistema Nervoso Periférico/citologia , Pressão , Células de Schwann/metabolismo , Células de Schwann/ultraestrutura , Nervo Isquiático/transplante , Nervo Isquiático/ultraestruturaRESUMO
We show that normal peripheral nerve myelination depends on strict dosage of the most abundantly expressed myelin gene, myelin protein zero (Mpz). Transgenic mice containing extra copies of Mpz manifested a dose-dependent, dysmyelinating neuropathy, ranging from transient perinatal hypomyelination to arrested myelination and impaired sorting of axons by Schwann cells. Myelination was restored by breeding the transgene into the Mpz-null background, demonstrating that dysmyelination does not result from a structural alteration or Schwann cell-extrinsic effect of the transgenic P(0) glycoprotein. Mpz mRNA overexpression ranged from 30-700%, whereas an increased level of P(0) protein was detected only in nerves of low copy-number animals. Breeding experiments placed the threshold for dysmyelination between 30 and 80% Mpz overexpression. These data reveal new points in nerve development at which Schwann cells are susceptible to increased gene dosage, and suggest a novel basis for hereditary neuropathy.
Assuntos
Doenças Desmielinizantes/congênito , Dosagem de Genes , Proteína P0 da Mielina/biossíntese , Proteína P0 da Mielina/genética , Doenças do Sistema Nervoso Periférico/congênito , Animais , Western Blotting , Doenças Desmielinizantes/genética , Doenças Desmielinizantes/metabolismo , Doenças Desmielinizantes/patologia , Regulação da Expressão Gênica , Camundongos , Camundongos Endogâmicos BALB C , Camundongos Transgênicos , Proteína Básica da Mielina/biossíntese , Proteína Básica da Mielina/genética , Proteínas da Mielina/biossíntese , Proteínas da Mielina/genética , Especificidade de Órgãos , Doenças do Sistema Nervoso Periférico/genética , Doenças do Sistema Nervoso Periférico/metabolismo , Doenças do Sistema Nervoso Periférico/patologia , Isoformas de Proteínas/biossíntese , Isoformas de Proteínas/genética , RNA Mensageiro/biossíntese , Reação em Cadeia da Polimerase Via Transcriptase Reversa , Células de Schwann/metabolismo , Células de Schwann/ultraestrutura , Nervo Isquiático/metabolismo , Nervo Isquiático/ultraestruturaRESUMO
POU proteins have been shown to transcriptionally active cell-specific genes and to participate in the determination of cell fate. It is therefore thought that these proteins function in development through the stable activation of genes that define specific developmental pathways. Evidence is provided here for an alternative mode of action. The primary structure of SCIP, a POU protein expressed by developing Schwann cells of the peripheral nervous system, was deduced and SCIP activity was studied. Both in normal development and in response to nerve transection, SCIP expression was transiently activated only during the period of rapid cell division that separates the premyelinating and myelinating phases of Schwann cell differentiation. In cotransfection assays, SCIP acted as a transcriptional repressor of myelin-specific genes.
Assuntos
Genes Homeobox/fisiologia , Proteínas do Tecido Nervoso/fisiologia , Proteínas Repressoras/fisiologia , Células de Schwann/citologia , Fatores de Transcrição/fisiologia , Sequência de Aminoácidos , Animais , Diferenciação Celular/genética , Clonagem Molecular , AMP Cíclico/fisiologia , Regulação da Expressão Gênica , Biblioteca Gênica , Genes Homeobox/genética , Dados de Sequência Molecular , Bainha de Mielina/metabolismo , Proteínas do Tecido Nervoso/genética , Fator 6 de Transcrição de Octâmero , Ratos , Proteínas Repressoras/genética , Células de Schwann/metabolismo , Fatores de Transcrição/genética , TransfecçãoRESUMO
Humoral immunity is important for protection against viral infection and neutralization of extracellular virus, but clearance of virus from infected tissues is thought to be mediated solely by cellular immunity. However, in a SCID mouse model of persistent alphavirus encephalomyelitis, adoptive transfer of hyperimmune serum resulted in clearance of infectious virus and viral RNA from the nervous system, whereas adoptive transfer of sensitized T lymphocytes had no effect on viral replication. Three monoclonal antibodies to two different epitopes on the E2 envelope glycoprotein mediated viral clearance. Treatment of alphavirus-infected primary cultured rat neurons with these monoclonal antibodies to E2 resulted in decreased viral protein synthesis, followed by gradual termination of mature infectious virion production. Thus, antibody can mediate clearance of alphavirus infection from neurons by restricting viral gene expression.
Assuntos
Alphavirus/fisiologia , Anticorpos Monoclonais/uso terapêutico , Sistema Nervoso Central/microbiologia , Encefalomielite/imunologia , Imunoterapia Adotiva , Neurônios/microbiologia , Linfócitos T/imunologia , Infecções por Togaviridae/imunologia , Alphavirus/imunologia , Alphavirus/isolamento & purificação , Animais , Sistema Nervoso Central/imunologia , Encefalomielite/microbiologia , Encefalomielite/terapia , Camundongos , Camundongos Endogâmicos , Camundongos SCID , Neurônios/imunologia , RNA Viral/isolamento & purificação , Infecções por Togaviridae/terapia , Replicação ViralRESUMO
Alzheimer's disease is characterized by widespread deposition of amyloid in the central nervous system. The 4-kilodalton amyloid beta protein is derived from a larger amyloid precursor protein and forms amyloid deposits in the brain by an unknown pathological mechanism. Except for aged nonhuman primates, there is no animal model for Alzheimer's disease. Transgenic mice expressing amyloid beta protein in the brain could provide such a model. To investigate this possibility, the 4-kilodalton human amyloid beta protein was expressed under the control of the promoter of the human amyloid precursor protein in two lines of transgenic mice. Amyloid beta protein accumulated in the dendrites of some but not all hippocampal neurons in 1-year-old transgenic mice. Aggregates of the amyloid beta protein formed amyloid-like fibrils that are similar in appearance to those in the brains of patients with Alzheimer's disease.
Assuntos
Doença de Alzheimer/genética , Peptídeos beta-Amiloides/genética , Encéfalo/metabolismo , Doença de Alzheimer/patologia , Peptídeos beta-Amiloides/análise , Animais , Sequência de Bases , Encéfalo/patologia , DNA/genética , Hipocampo/ultraestrutura , Humanos , Camundongos , Camundongos Transgênicos , Microscopia Eletrônica , Dados de Sequência Molecular , Neurofibrilas/ultraestrutura , Sondas de Oligonucleotídeos , Mapeamento por RestriçãoRESUMO
Chemokines (pro-inflammatory chemoattractant cytokines) are expressed in pathological conditions of the central nervous system (CNS). Previous studies suggested that the CNS is relatively resistant to leukocyte diapedesis after chemokine injection, leaving their functional role unresolved. The CNS function of N51/KC, a neutrophil-selective chemokine, was addressed by expressing N51/KC under control of the myelin basic protein (MBP) promoter in transgenic (tg) mice (MBP-N51/KC mice). CNS-specific N51/KC expression produced remarkable neutrophil infiltration into perivascular, meningeal, and parenchymal sites, demonstrating that this chemokine exerts the multiple functions in vivo required to recruit leukocytes into the CNS. MBP-N5 1/KC mice represent an incisive model for the molecular dissection of neutrophil entry into the CNS. Unexpectedly, MBP-N51/KC mice developed a neurological syndrome of pronounced postural instability and rigidity at high frequency beginning at 40 days of age, well after peak chemokine expression. 68/182 mice in one tg fine were found dead before one year of age, with prominent neurological symptoms premortem in 26 (38%). Florid microglial activation and blood-brain barrier disruption without dysmyelination were the major neuropathological alterations. Late-onset neurological symptoms in MBP-N51/KC mice may indicate unanticipated consequences of CNS chemokine expression.
Assuntos
Quimiocinas CXC , Fatores Quimiotáticos/biossíntese , Citocinas/biossíntese , Substâncias de Crescimento/biossíntese , Peptídeos e Proteínas de Sinalização Intercelular , Doenças do Sistema Nervoso/fisiopatologia , Neuroglia/fisiologia , Neutrófilos/fisiologia , Oligodendroglia/fisiologia , Animais , Astrócitos/patologia , Sequência de Bases , Encéfalo/patologia , Encéfalo/fisiopatologia , Quimiocina CXCL1 , Quimiocinas , Fatores Quimiotáticos/genética , Citocinas/genética , Primers do DNA , Feminino , Substâncias de Crescimento/genética , Íntrons , Masculino , Camundongos , Camundongos Endogâmicos C57BL , Camundongos Endogâmicos DBA , Camundongos Endogâmicos ICR , Camundongos Transgênicos , Microscopia Eletrônica , Dados de Sequência Molecular , Proteína Básica da Mielina/biossíntese , Proteína Básica da Mielina/genética , Doenças do Sistema Nervoso/genética , Doenças do Sistema Nervoso/patologia , Neuroglia/patologia , Neutrófilos/patologia , Neutrófilos/ultraestrutura , Oligodendroglia/patologia , Reação em Cadeia da Polimerase , Postura , Regiões Promotoras Genéticas , Proteínas Recombinantes de Fusão/biossíntese , Mapeamento por RestriçãoRESUMO
Fragile X-associated tremor/ataxia syndrome (FXTAS) is an adult-onset neurodegenerative disorder that affects carriers, principally males, of premutation alleles (55-200 CGG repeats) of the fragile X mental retardation 1 (FMR1) gene. Clinical features of FXTAS include progressive intention tremor and gait ataxia, accompanied by characteristic white matter abnormalities on MRI. The neuropathological hallmark of FXTAS is an intranuclear inclusion, present in both neurons and astrocytes throughout the CNS. Prior to the current work, the nature of the associations between inclusion loads and molecular measures (e.g. CGG repeat) was not defined. Post-mortem brain and spinal cord tissue has been examined for gross and microscopic pathology in a series of 11 FXTAS cases (males, age 67-87 years at the time of death). Quantitative counts of inclusion numbers were performed in various brain regions in both neurons and astrocytes. Inclusion counts were compared with specific molecular (CGG repeat, FMR1 mRNA level) and clinical (age of onset, age of death) parameters. In the current series, the three most prominent neuropathological characteristics are (i) significant cerebral and cerebellar white matter disease, (ii) associated astrocytic pathology with dramatically enlarged inclusion-bearing astrocytes prominent in cerebral white matter and (iii) the presence of intranuclear inclusions in both brain and spinal cord. The pattern of white matter pathology is distinct from that associated with hypertensive vascular disease and other diseases of white matter. Spongiosis was present in the middle cerebellar peduncles in seven of the eight cases in which those tissues were available for study. There is inclusion formation in cranial nerve nucleus XII and in autonomic neurons of the spinal cord. The most striking finding is the highly significant association between the number of CGG repeats and the numbers of intranuclear inclusions in both neurons and astrocytes, indicating that the CGG repeat is a powerful predictor of neurological involvement in males, both clinically (age of death) and neuropathologically (number of inclusions).
Assuntos
Astrócitos/ultraestrutura , Ataxia/patologia , Síndrome do Cromossomo X Frágil/patologia , Corpos de Inclusão Intranuclear/ultraestrutura , Neurônios/ultraestrutura , Tremor/patologia , Idade de Início , Idoso , Idoso de 80 Anos ou mais , Análise de Variância , Ataxia/genética , Encéfalo/patologia , Estudos de Casos e Controles , Contagem de Células , Proteína do X Frágil da Deficiência Intelectual/genética , Síndrome do Cromossomo X Frágil/genética , Humanos , Masculino , Medula Espinal/patologia , Tremor/genética , Expansão das Repetições de TrinucleotídeosRESUMO
We describe a major glial cell population in the central nervous system (CNS) that can be identified by the expression of 2 cell surface molecules, the NG2 proteoglycan and the alpha receptor for platelet-derived growth factor (PDGF alphaR). In vitro and in the developing brain in vivo, NG2 and PDGF alphaR are expressed on oligodendrocyte progenitor cells but are down-regulated as the progenitor cells differentiate into mature oligodendrocytes. In the mature CNS, numerous NG2+/PDGF alphaR+ cells with extensive arborization of their cell processes are found ubiquitously long after oligodendrocytes are generated. NG2+ cells in the mature CNS do not express antigens specific to mature oligodendrocytes, astrocytes, microglia, or neurons, suggesting that they are a novel population of glial cells. Recently NG2+ cells in the adult CNS have been shown to undergo proliferation and morphological changes in response to a variety of stimuli, such as demyelination and inflammation, suggesting that they are dynamic cells capable of responding to changes in the environment. Furthermore, high levels of NG2+ and PDGF alphaR are expressed on oligodendroglioma cells, raising the possibility that the NG2+/PDGF alphaR+ cells in the mature CNS contribute to glial neoplasm.