Performance, meat quality, meat mineral contents and caecal microbial population responses to humic substances administered in drinking water in broilers.

This study was conducted to examine the effect of different levels of humic substances (HS) administered in drinking water on caecal microflora and mineral composition and colour characteristics of breast and thigh meats and the growth performance, carcass and gastrointestinal tract (GIT) traits of broiler chicks. A total of 480 3-d-old broiler chickens were randomly allocated to 4 treatments with 4 cages per treatment and 30 bird (15 males and 15 females) chicks per cage. All birds were fed on commercial basal diet. The control birds (HS0) received drinking water with no additions, whereas birds in the other treatment groups received a drinking water with 7.5 (HS7.5), 15.0 (HS15.0) and 22.5 (HS22.5) g/kg HS. Mush feed were provided on an ad libitum basis. Body weight and feed intake of broilers were determined at d 0, 21, and 42, and feed conversion ratio was calculated. On d 42, 4 broilers (2 males and 2 females) from each cage were slaughtered and the breast and thigh meats were collected for mineral composition and quality measurements. Performance, carcass and GIT traits and caecal microbial population of broiler chicks at d 42 were not affected by the dietary treatments. The lightness (L*) of breast and thigh meat decreased in broilers supplemented with 15 and 22.5 g/kg HS in drinking water. Although the redness (a*) of breast meat increased, yellowness of thigh meat decreased in broilers supplemented with 15 and 22.5 g/kg HS in drinking water (P < 0.05). In conclusion, the 15 and 22.5 g/kg HS administration in drinking water can be applied for broiler chicks to maintain growth performance and improve meat quality without changing caecal microflora.

Evaluation of meat quality from 3 turkey genotypes reared with or without outdoor access.

To evaluate color [lightness (L*), redness (a*), and yellowness (b*)], water-holding capacity (WHC), and pH values, and for proximate analysis of breast and thigh meats from slow-growing (Bronze; B), fast-growing (Hybrid; H), and medium-growing (crosses; H × B) turkey genotypes raised with or without outdoor access, 36 turkeys (2 females and 2 males from each replicate) per housing system or 12, 16, and 8 turkeys per B, H, and H × B genotype, slaughtered at 17 and 21 wk of age, respectively, were used. Therefore, data were analyzed as a factorial arrangement (2 × 3 × 2 × 2) of treatments. All birds were provided with the same starter, grower, and finisher feeds. Muscle samples were collected at 12 h postmortem for evaluation of meat quality and proximate analysis. Outdoor access increased the a* value and protein content of the breast muscle (P < 0.05) and the b* value of the thigh muscle (P < 0.01). The B and H genotypes had higher (P < 0.01) L* values for the breast meat than did the H × B genotype, whereas the B genotype had lower a* (P < 0.01) and pH (P < 0.01) values for the breast meat or a higher (P < 0.05) pH value for the thigh muscle compared with the H genotype. The breast meat of the B genotype was more yellow (P < 0.01) than that of the H and H × B genotype. Thigh meat from the H genotype had a higher L* value and a lower a* value than did thigh meat from the other genotypes (P < 0.01). Thigh meat from the H × B genotype was higher in protein and lower in fat than was thigh meat from the B and H genotypes, respectively (P < 0.05). No interaction effect of housing system and genotype was observed on the parameters studied (P > 0.05). These results show that housing system did not affect the main quality parameters (pH, water-holding capacity, and L* values) of either muscle, and that genotype created more differences in terms of these parameters.

Effects of humic substances supplementation provided through drinking water on performance, carcass traits and meat quality of broilers.

The present study aimed at evaluating the effects of different doses of humic substances (HS) in waterer pan on broiler performances and meat quality. For the trial, 480 chicks (ROSS 308) were allocated into four groups (HS0, HS150, HS300 and HS450) of 120 equally mixed-sex birds, each receiving a drinking water supplemented with 0, 150, 300 and 450 ppm of a humic acid (provided by 0.0%, 0.5%, 1.0% and 1.5% of HS, v/v) respectively. At the age of 21 and 42 days, feed consumption and live weight were taken. At the age of 42 days, 16 birds per treatment were slaughtered, dressing percentages, digestive tract traits were evaluated and samples of breast (pectoralis major) and thigh (iliotibialis) meat were taken to evaluate the changes in colour characteristics (CIE L*, a* and b*) and chemical composition. The body weight gain increased by the HS300 in relation to the HS0 and HS450, and decreased by the HS450 in relation to the HS0. The feed consumption of birds in the HS450 and feed efficiency of birds in the HS300 were lower and higher than those in the other treatments respectively. The carcass weight of broilers in the HS150 and HS300 were higher than those of in the HS0 and HS450. The carcass weight decreased by the HS450 in relation to the other treatments. The dressing percentage increased by the HS150 in relation to the HS450. The HS450 resulted in breast and thigh meat colour that were lighter than those found in the HS0 birds. The HS450 increased fat content of thigh meat in relation to the HS0. It was concluded that the humic acid supplementation at 300 ppm and 450 ppm appears to have a measurable impact on live performance improving feed efficiency and lightness of breast and thigh meat colours in broilers respectively.

Radiometric characterization of zeolite minerals used in many industries and assessment of radiological risks.

Zeolites are important additives materials which are widely used in different industries such as construction, paper, paint, agriculture, textile, and fertilizer. In this study, radiometric characterization of natural zeolite samples collected from four zeolite open quarries in Western Anatolia in Turkey were determined using a gamma-ray spectrometry with high purity germanium detector. The radiological risks due to the use of zeolite samples as raw materials in cement and concrete production was assessed for adult people by estimating activity concentration index and annual effective dose due to external exposure in indoor. Also, annual effective radiation dose in outdoor and the corresponding lifetime cancer risk were estimated for quarry workers. Average activity concentrations of 226Ra, 232Th and 40K measured in seventy-seven zeolite samples were found as 85 ±â€¯4, 129 ±â€¯2 and 1030 ±â€¯24 Bq kg-1, respectively. The radon emanation factor and radon mass exhalation rate of zeolite samples varied from 1 to 9% with an average of 4% and 1.8-15.1 µBq kg-1 s-1 with an average of 7.2 ±â€¯0.3 µBq kg-1 s-1, respectively. The data were compared with criteria or limit values. The results revealed that usage of the examined zeolite samples as building raw materials would not cause any significant radiological risk.

Assessment of the natural radioactivity and radiological hazards in Turkish cement and its raw materials.

The natural radioactivity due to presence of (226)Ra, (232)Th and (40)K radionuclides in raw materials, intermediate products (clinker) and end products (22 different cement types) was measured using a gamma-ray spectrometry with HPGe detector. The specific radioactivity of (226)Ra, (232)Th and (40)K in the analyzed cement samples ranged from 12.5+/-0.3 to 162.5+/-1.7Bqkg(-1) with a mean of 40.5+/-26.7Bqkg(-1), 6.7+/-0.3 to 124.9+/-2.5Bqkg(-1) with a mean of 26.1+/-18.9Bqkg(-1) and 64.4+/-2.3 to 679.3+/-18.2Bqkg(-1) with a mean of 267.1+/-102.4Bqkg(-1), respectively. The radium equivalent activity (Ra(eq)), the gamma-index, the emanation coefficient, the (222)Rn mass exhalation rate and the indoor absorbed dose rate were estimated for the radiation hazard of the natural radioactivity in all samples. The calculated Ra(eq) values of cement samples (37.2+/-8.7-331.1+/-15.5Bqkg(-1) with a mean of 98.3+/-53.8) are lower than the limit of 370Bqkg(-1) set for building materials. The Ra(eq) values were compared with the corresponding values for cement of different countries. The mean indoor absorbed dose rate is slightly higher than the population-weighted average of 84nGyh(-1).

Radiological significance of cement used in building construction in Turkey.

The activity concentration of (226)Ra, (232)Th and (40)K in seven cement types from different factories and grinding plants were measured using a gamma ray spectrometry with HPGe detector. The average activity concentrations observed in the studied cement samples (all from 141 samples) were 40.0 +/- 27.1, 28.0 +/- 20.9 and 248.3 +/- 95.0 Bq kg(-1) for (226)Ra, (232)Th and (40)K, respectively. The radium equivalent activity (Ra(eq)), the representative level index, the indoor absorbed dose rate and the corresponding annual effective dose were estimated for the potential radiological hazard of the cement. The Ra(eq) values were compared with the corresponding values for cement of different countries. The mean indoor absorbed dose rate (87.4 +/- 48.5 nGy h(-1)) is slightly higher than the population-weighted average of 84 nGy h(-1), whereas the corresponding effective dose rate (0.4 +/- 0.2 mSv y(-1)) is lower than the dose criterion of 1 mSv y(-1). The obtained results indicate no significant radiological hazards arise from using Turkish cement in building construction.

Radiological evaluation of the use of clay brick and pumice brick as a structural building material.

In this study, clay brick (CBRICK) and pumice brick (PBRICK) samples used as structural material in the construction of dwellings, schools, workplaces and factories in Turkey were compared with each other from a radiological viewpoint. The activity concentrations of 226Ra, 232Th and 40K naturally occurring in CBRICK and PBRICK samples collected from different regions of Turkey were determined by using a gamma-ray spectrometer with a high purity germanium detector (HPGe). The average activity concentrations of 226Ra, 232Th and 40K in CBRICK and PBRICK samples were found as 35.4 ±â€¯3.3, 37.5 ±â€¯2.4 and 593.7 ±â€¯42.7 Bq kg-1 and 81.9 ±â€¯2.5, 65.8 ±â€¯6.0 and 1066.0 ±â€¯46.6 Bq kg-1, respectively. The radon surface exhalation rate (EXS) and radon mass exhalation rate (EXM) of CBRICK and PBRICK samples were measured by using an active radon gas analyzer with an accumulation container. The average value of EXS and EXM of CBRICK and PBRICK samples found as 45.9 ±â€¯2.9 mBq m-2 h-1 and 3.7 ±â€¯2.9 mBq kg-1 h-1 and 100.9 ±â€¯4.7 mBq m-2 h-1 and 9.9 ±â€¯0.5 mBq kg-1 h-1, respectively. Radiological parameters related to external and internal exposure to members of the public such as the radiation protection index, alpha index, and indoor absorbed gamma radiation dose rate and the corresponding annual effective dose from external exposure, annual effective dose from inhalation of radon, and the lifetime cancer risk were estimated for CBRICK and PBRICK samples. The results were compared with each other and with the international recommended limits or criteria. The results reveal that the average values measured and estimated for CBRICK samples are approximately two times lower than those measured and estimated for PBRICK samples. Thus, from the radiological viewpoint, clay brick is preferable to pumice brick as a structural material in the building sector.

Torsade de pointes associated with moxifloxacin: a rare but potentially fatal adverse event.

Torsade de pointes occuring due to a long QT interval is a rare but potentially fatal arrhythmia. Acquired long QT develops most commonly because of drugs that prolong ventricular repolarization. It has been reported that fluoroquinolone antimicrobials prolong the corrected QT interval but rarely cause torsade de pointes. A patient with torsade de pointes risk factors (female sex, advanced age, extreme bradycardia and renal failure) who developed the condition on the fourth day of 400 mg/day of oral moxifloxacin treatment is presented. After the moxifloxacin was stopped, the corrected QT interval normalized and a permanent cardiac pacemaker was implanted. During 11 months of follow-up, arrhythmia did not recur.

Natural radioactivity measurement in pumice samples used raw materials in Turkey.

The activity concentrations of 232Th, 226Ra, and 40K in different pumice samples have been determined by high-resolution gamma-ray spectrometry using a 110% HpGe detector. The radium equivalent activities (Raeq), external hazard index (Hex), and internal hazard index (Hin) associated with the natural radionuclides and representative level index (Igamma r) are calculated to assess the radiation hazard of the natural radioactivity in the pumice samples. The mean values of the measured radioactivity concentrations of 232Th, 226Ra, and 40K for pumice samples from the region of lakes (ROL) are 232.4+/-8.0, 196.9+/-7.8, and 1325.8+/-20.4 Bqkg(-1) and for pumice samples from Cukurova region (CR) 16.3+/-4.0, 16.1+/-4.9, and 479.7+/-170.4 Bqkg(-1), respectively. The calculated Raeq values vary from 435.9+/-12.5 to 883.6+/-41.5 Bqkg(-1) with a mean of 630.9+/-20.2 Bqkg(-1) for the ROL samples and from 49.7+/-3.3 to 101.9+/-7.2 Bqkg(-1) with a mean of 76.3+/-23.7 Bqkg(-1) for the CR samples. For the ROL samples, Raeq are above the limit of 370 Bqkg(-1), equivalent to external gamma dose of 1.5 mSv yr(-1), recommended for the safe use of construction materials by NEA-OECD, while for the CR samples, Raeq values are lower than the limit.

Environmental evaluation of natural radioactivity in soil near a lignite-burning power plant in Turkey.

Natural radionuclides are released into the environment together with fly ash from the coal-burning power plant and cause an increase in the natural radioactivity in environmental samples. The study concerns to the evaluation the influence of Kangal lignite-burning power plant (LBPP) with a power of 457 MWe, which has been in operation since 1989, on natural radionuclide a concentration in surface soil samples around it. Activity concentrations of natural radionuclides (226Ra, 232Th, 40K and 222Rn) in the soil samples, and emanation coefficient (EC) and mass (ERM) and surface (ERS) exhalation rate of radon were determined by using a gamma-ray spectrometer with an HPGe detector. The average values of 226Ra, 232Th, 40K and 222Rn, EC, ERM and ERS were found as 37±5, 17±3, 222±30Bqkg-1 and 9±1kBqm-3, 12%, 12.1 µBq kg-1 s-1 and 7.1mBqm-2 s-1, respectively. Absorbed gamma dose rate in outdoor air and the corresponding effective dose rate from external exposure and excess lifetime cancer risk were estimated to evaluate radiological hazards for human population. The results revealed that the Kangal LBPP has caused a small increment in 226Ra concentration in the studied area. No influence was observed for 232Th and 40K.

Determination of natural radioactivity by gross alpha and beta measurements in ground water samples.

In this study, the activity concentrations of the gross α and ß in ground water samples collected from the different drilled wells in Nevsehir province were measured to assess annual effective dose due to the ingestion of the water samples. Nevsehir province is one of the major cities of Cappadocia Region which is a popular tourist destination as it has many areas with unique geological, historic, and cultural features. Sampling and measurements were carried out in the autumn of 2011 and the spring of 2012. The values of the activity concentrations of the gross α and ß measured in the water samples ranged from 80 to 380 mBq L(-1) with a mean of 192 mBq L(-1) and 120-3470 mBq L(-1) with a mean of 579 mBq L(-1) respectively. All values of the gross α were lower than the limit value of 500 mBq L(-1) while two ground water samples were found to have gross ß activity concentrations of greater than 1000 mBq L(-1). Therefore two water samples were the subject of further radioisotope-specific analysis. The obtained result indicated that the elevated activity concentrations of the gross ß in these water samples are dominated by (40)K activity. Annual effective doses ranged from 0.04 to 0.20 mSv y(-1).

Investigation of the activity level and radiological impacts of naturally occurring radionuclides in blast furnace slag.

The activity level and possible radiological impacts of naturally occurring radionuclides on the health of workers and members of the public, as a result of utilisation of blast furnace slag (BFS) samples as a substitute for aggregate in road construction were investigated by using a gamma-ray spectrometer and potential exposure scenarios given in Radiation Protection 122. The mean activity concentrations of the (226)Ra, (232)Th and (40)K in BFS samples were found to be 152.4, 54.9 and 183.1 Bq kg(-1), respectively. These values are compared with typical values measured in BFS samples from the European Union countries, which are 270, 70 and 240 Bq kg(-1) for (226)Ra, (232)Th and (40)K, respectively. The values of radium equivalent activity index calculated for BFS samples were within the recommended safety limits. The highest total annual effective doses evaluated as 0.9 and 0.4 mSv y(-1) for members of the public and workers, respectively, were lower than the annual limit of 1 mSv y(-1).

A survey of distribution of terrestrial radionuclides in surface soil samples in and around the Osmaniyeprovince, Turkey.

The purpose of this survey was to determine the distribution of terrestrial radionuclides ((238)U, (232)Th and (40)K) in surface soil samples collected from different locations in and around Osmaniye and assess the radiological implications of outdoor external exposure. The activity concentrations of (238)U, (232)Th and (40)K in soil samples ranged from 2.6 to 38.1 Bq kg(-1) with a mean of 10.4±0.7 Bq kg(-1), 3.7-40.5 Bq kg(-1) with a mean of 12.2±0.7 Bq kg(-1) and 30.0-639.7 Bq kg(-1) with a mean of 243.4±12.8 Bq kg(-1), respectively. The concentrations of these radionuclides, presented in this article, were compared with those obtained from similar studies related to Turkey reported in the literature as well as earth's crust average values. The results of the activity concentrations were used to assess the external absorbed gamma dose rate (GDR) in outdoor air and the annual effective dose (AED). The outdoor absorbed GDRs fluctuate from 5.2 to 53.6 nGy h(-1) with a mean of 22.4±9.5. The corresponding mean AED is found to be 0.03 mSv.

Radioactivity measurement of primordial radionuclides in and dose evaluation from marble and glazed tiles used as covering building materials in Turkey.

Measurements of the natural radioactivity arising from primordial radionuclides ((226)Ra, (232)Th and (40)K) in marble and glazed tile samples used covering building materials in Turkey were carried out by gamma-ray spectrometer with a high purity germanium detector. The mean activity concentrations of the (226)Ra, (232)Th and (40)K in marble and glazed tile samples were found as 8.2, 5.5 and 58.1 Bq kg(-1) and 81.2, 65.4 and 450.1 Bq kg(-1), respectively. The radiation doses received by occupants of buildings in which the sample marble and glazed tiles might be used are estimated using measured activity concentrations of constituent primordial radionuclides and dose conversion factors evaluated by the European Commission from models of tile use. Results obtained are presented for each radionuclide, analysed and compared with relevant national and international legislation, guidance and report, and with the results obtained from other studies. Results show that the use of such decorative building materials in the construction of domestic homes or workplaces in Turkey is unlikely to lead to any significant radiation exposure to the occupants.

Car-borne survey of natural background gamma dose rate in Çanakkale region, Turkey.

Natural background gamma radiation was measured along roads in the environs of Çanakkale region by using a car-borne spectrometer system with a plastic gamma radiation detector. In addition, activity concentrations of ²³8U, ²²6Ra, ²³²Th and 4°K in soil samples from the Çanakkale region were determined by using a gamma spectrometer with an HPGe detector. A total of 92,856 data of the background gamma dose rate were collected for the Çanakkale region. The background gamma dose rate of the Çanakkale region was mapped using ArcGIS software, applying the geostatistical inverse distance-weighted method. The average and population-weighted average of the gamma dose are 55.4 and 40.6 nGy h⁻¹, respectively. The corresponding average annual effective dose to the public ranged from 26.6 to 96.8 µSv.

Natural radionuclide content and radiological hazard associated with usage of quartzite sand samples from Ovacik-Silifke-Mersin open pit as building material in Turkey.

Activity concentrations of (238)U, (232)Th, (226)Ra and (40)K in quartzite sand samples collected from the Ovacik-Silifke-Mersin open pit located in the East Mediterranean region of Turkey were determined by using a gamma-ray spectrometry technique. The mean activity concentrations of the (238)U, (232)Th, (226)Ra and (40)K in quartzite sand samples were found as 81.7 +/- 22.9 Bq kg(-1), 6.3 +/- 2.8 Bq kg(-1), 77.5 +/- 24.3 Bq kg(-1)and 140.0 +/- 124.1 Bq kg(-1), respectively. The gamma index (I(gamma)), the internal exposure index (I(alpha)), the indoor absorbed dose rate (D(in)) and the corresponding annual effective dose (H(in)) were evaluated for the public exposure to radiological hazard arising due to the use of quartzite sand samples as building material. The values of I(gamma), I(alpha), D(in) and H(in) ranged from 0.20 to 0.75, with a mean of 0.34 +/- 0.11, 0.23 to 0.77 with a mean of 0.39 +/- 0.12, 58.27 to 201.51 nGy h(-1) with a mean of 93.33 +/- 27.63 nGy h(-1) and 0.29 to 0.99 mSv with a mean of 0.46 +/- 0.14 mSv, respectively.

Measurement of the natural radioactivity in building materials used in Ankara and assessment of external doses.

A total of 183 samples of 20 different commonly used structural and covering building materials were collected from housing and other building construction sites and from suppliers in Ankara to measure the natural radioactivity due to the presence of (226)Ra, (232)Th and (40)K. The measurements were carried out using gamma-ray spectrometry with two HPGe detectors. The specific activities of the different building materials studied varied from 0.5 +/- 0.1 to 144.9 +/- 4.9 Bq kg(-1), 0.6 +/- 0.2 to 169.9 +/- 6.6 Bq kg(-1) and 2.0 +/- 0.1 to 1792.3 +/- 60.8 Bq kg(-1) for (226)Ra, (232)Th and (40)K, respectively. The results show that the lowest mean values of the specific activity of (226)Ra, (232)Th and (40)K are 0.8 +/- 0.5, 0.9 +/- 0.4 and 4.1 +/- 1.4 Bq kg(-1), respectively, measured in travertine tile while the highest mean values of the specific activity of the same radionuclides are 78.5 +/- 18.1 (ceramic wall tile), 77.4 +/- 53.0 (granite tile) and 923.4 +/- 161.0 (white brick), respectively. The radium equivalent activity (Ra(eq)), the gamma-index, the indoor absorbed dose rate and the corresponding annual effective dose were evaluated to assess the potential radiological hazard associated with these building materials. The mean values of the gamma-index and the estimated annual effective dose due to external gamma radiation inside the room for structural building materials ranged from 0.15 to 0.89 and 0.2 to 1.1 mSv, respectively. Applying criteria recently recommended for building materials in the literature, four materials meet the exemption annual dose criterion of 0.3 mSv, five materials meet the annual dose limit of 1 mSv and only one material slightly exceeds this limit. The mean values of the gamma-index for all building materials were lower than the upper limit of 1.

How previous angina influences early prognosis of patients with acute myocardial infarction.

BACKGROUND: There is little information about how previous angina influences the complications of myocardial infarction and also contradictory results have been reported. OBJECTIVE: To compare the risk factors for myocardial infarction, complications, performance of left ventricle, and coronary angiography findings of patients who had suffered acute myocardial infarction with those for patients who had not. METHODS: We studied 600 patients diagnosed to have suffered acute myocardial infarction. Patients are grouped into those having previously had angina for at least 1 month preceding acute myocardial infarction (group I, n = 308 patients; 223 men and 85 women, mean age 60.4 +/- 10.6 years) and those who had not had angina (group II, n = 292 patients; 221 men and 71 women, mean age 58 +/- 9 years). The risk factors, complications (cardiogenic shock, heart failure, disturbances of rhythm and conduction, cardiac rupture and death), left-ventricle ejection fraction, and echocardiography and coronary angiographic findings during hospitalization are compared. RESULTS: There was no difference with respect to localization of myocardial infarction (anterior, inferior, and non-Q) between groups I and II (P> 0.05). Hypertension in members of group I was higher (P < 0.05). There was no statistically significant difference with respect to diabetes mellitus, hypercholesterolemia and cigarette smoking (P > 0.05). Heart failure (P< 0.05), cardiogenic shock (P< 0.01), incidence of ventricular premature systole > 3/min (P< 0.001) and atrial fibrillation (P< 0.05) were seen more prevalently in group II than they were in group I. There was no difference between the two groups with respect to bundle-branch blockage and third-degree atrioventricular blockage. Incidences of ventricular fibrillation, rupture of interventricular septum (IVS) and death in hospital were higher in group II (6.2 versus 3.6%, 6.2 versus 3.2%, 2.1 versus 0.6%) but were not statistically significant. Coronary angiography detected no statistically significant difference with respect to disease in left main coronary artery, and one-vessel and two-vessel disease; but three-vessel disease was significantly more prevalent in group II (P < 0.01). CONCLUSION: Heart failure, cardiogenic shock, arrhythmia (more than three VPS within 1 min and atrial fibrillation), and three-vessel disease detected by coronary angiography were found more often in the myocardial infarct patients without previous angina and these differences were statistically significant. In-hospital mortality and cardiac rupture were also found more commonly in this group and ejection fractions measured by echocardiography were found to be less, but these differences were statistically insignificant.

Spontaneous clearance of hepatitis C virus infection post-liver transplantation is associated with rapidly changing quasispecies: a single case report.

Hepatitis C virus (HCV) clearance post-liver transplantation is uncommon. This is a case report of a patient who, after liver transplantation, developed cholestatic hepatitis characterized by severe graft dysfunction, in conjunction with high viral load. This was, however, followed by viral clearance and normalization of allograft function. The clinical features of this case and the quasispecies patterns during the illness and the clearance periods are described. In addition, management implications in terms of immunosuppressive therapy are discussed.