Comparison of NIRS exercise intensity thresholds with maximal lactate steady state, critical power and rowing performance.

This study aimed to compare the intensity of deoxygenated haemoglobin concentration ([HHb]) and tissue saturation index (TSI) breakpoints ([HHb]-BP and TSI-BP) with maximal lactate steady state (MLSS) and critical power (CP), and to describe their association with 2000-m rowing ergometer performance. Fourteen male rowers performed on a rowing ergometer: I) a discontinuous incremental test with 3-min stages (INC3); II) a continuous incremental test with 1-min stages (INC1); III) constant workload tests to determine MLSS; and IV) performance tests of 500 m, 1000 m, 2000 m and 6000 m to determine CP.CP (257 ± 39 W; 3.79 ± 4.1 L · min-1) was higher than [HHb]-BP3 (205 ± 26 W; 3.48 ± 2.9 L · min-1), [HHb]-BP1 (207 ± 27 W; 3.27 ± 3.2 L · min-1), and TSI-BP3 (218 ± 31 W; 3.51 ± 3.0 L · min-1), but not higher than TSI-BP1 (222 ± 34 W; 3.43 ± 3.2 L · min-1). MLSS (187 ± 26 W; 3.33 ± 3.2 L · min-1) was lower than TSI-BP3 and TSI-BP1 for power output, but not different in any comparison for ⩒O2. The limits of agreement for power output and ⩒O2 suggest poor agreement among these thresholds. The low level of agreement compromises the use of [HHb]-BP and TSI-BP for estimating MLSS and CP; therefore, these thresholds should not be considered interchangeable.

Near-infrared spectroscopy-derived muscle V̇O2${\dot{V}_{{{\rm{O}}_{\rm{2}}}}}$ kinetics after moderate running exercise in healthy males: reliability and associations with parameters of aerobic fitness.

NEW FINDINGS: What is the central question of this study? What is the reliability of near-infrared spectroscopy-derived muscle oxygen uptake ( mV̇O2${\rm{m}}{\dot{V}_{{{\rm{O}}_{\rm{2}}}}}$ ) kinetics following running exercise and what is the relationship between the time constant of mV̇O2${\rm{m}}{\dot{V}_{{{\rm{O}}_{\rm{2}}}}}$ off-kinetics and parameters of aerobic fitness? What is the main finding and its importance? The time constant of mV̇O2${\rm{m}}{\dot{V}_{{{\rm{O}}_{\rm{2}}}}}$ kinetics in gastrocnemius following moderate running exercise presents good to excellent reliability. In addition, it was well correlated with parameters of aerobic fitness, such as maximal speed of an incremental test, ventilatory threshold and pulmonary V̇O2${\dot{V}_{{{\rm{O}}_{\rm{2}}}}}$ on-kinetics. Therefore, near-infrared spectroscopy-derived muscle oxidative capacity together with other physiological measurements may allow a concomitant local and systemic analysis of the components of the oxidative system. ABSTRACT: Near-infrared spectroscopy-derived muscle oxygen uptake ( mV̇O2${\rm{m}}{\dot{V}_{{{\rm{O}}_{\rm{2}}}}}$ ) kinetics following single-joint exercise has been used to assess muscle oxidative capacity. However, little evidence is available on the use of this technique following whole-body exercise. Therefore, this study aimed to assess the reliability of the NIRS-derived mV̇O2${\rm{m}}{\dot{V}_{{{\rm{O}}_{\rm{2}}}}}$ kinetics following running exercise and to investigate the relationship between the time constant of mV̇O2${\rm{m}}{\dot{V}_{{{\rm{O}}_{\rm{2}}}}}$ off-kinetics ( τmV̇O2$\tau {\rm{m}}{\dot{V}_{{{\rm{O}}_{\rm{2}}}}}$ ) and parameters of aerobic fitness. After an incremental test to determine V̇O2max${\dot{V}_{{{\rm{O}}_{\rm{2}}}{\rm{max}}}}$ , first (VT1 ) and second (VT2 ) ventilatory thresholds, and maximal speed (Smax ), 13 males (age = 21 ± 4 years; V̇O2max${\dot{V}_{{{\rm{O}}_{\rm{2}}}{\rm{max}}}}$  = 55.9 ± 3.4 ml kg-1  min-1 ) performed three sets (two on the first day and one on a subsequent day) of two repetitions of 6-min running exercise at 90%VT1 . The pulmonary V̇O2${\dot{V}_{{{\rm{O}}_{\rm{2}}}}}$ ( pV̇O2${\rm{p}}{\dot{V}_{{{\rm{O}}_{\rm{2}}}}}$ ) on-kinetics and mV̇O2${\rm{m}}{\dot{V}_{{{\rm{O}}_{\rm{2}}}}}$ off-kinetics in gastrocnemius were assessed. τmV̇O2$\tau {\rm{m}}{\dot{V}_{{{\rm{O}}_{\rm{2}}}}}$ presented no systematic change and satisfactory reliability (the standard error of the measurement (SEM) and intraclass correlation coefficient of 4.21 s and 0.49 for between transitions; and 2.65 s and 0.74 averaging τmV̇O2$\tau {\rm{m}}{\dot{V}_{{{\rm{O}}_{\rm{2}}}}}$ within each time set), with no difference (P > 0.3) between the within- (SEM = 2.92 s) and between-day variability (SEM = 2.78 s and 2.19 s between first vs. third set, and second vs. third set, respectively). τmV̇O2$\tau {\rm{m}}{\dot{V}_{{{\rm{O}}_{\rm{2}}}}}$ (28.5 ± 4.17 s) correlated significantly (P < 0.05) with Smax (r = -0.66), VT1 (r = -0.64) and time constant of the p V̇O2${\dot{V}_{{{\rm{O}}_{\rm{2}}}}}$ on-kinetics (r = 0.69). These findings indicate that NIRS-derived mV̇O2${\rm{m}}{\dot{V}_{{{\rm{O}}_{\rm{2}}}}}$ kinetics in the gastrocnemius following moderate running exercise is a useful and reliable parameter to assess muscle oxidative capacity.

Similar Time Near VO2max Regardless of Work Rate Manipulation in Cycling Interval Training.

The current study aimed to compare time spent above 90% V̇O2max (tV̇O2max) during 3 work-matched interval training protocols comprising 8×60-second exercise efforts with decreasing, increasing, or constant work rate distribution within each exercise interval. Ten healthy male subjects (age: 27.6±5.0 years; V̇O2max: 3.82±0.52 L•min-1) performed an incremental test to determine V̇O2max and peak power output (P max). During visits 2, 3, and 4, three work-matched interval training sessions comprising 8×60 s efforts: 60 s active recovery with the power output held constant (100%Pmax; ITCON), decreasing (from 110 to 90%Pmax; ITDEC), or increasing (from 90 to 110%Pmax; ITINC) linearly throughout each work interval. Time sustained above 90% of V̇O2max (tV̇O2max) or HRmax (tHRmax), blood lactate concentrations (BLC) and rating of perceived exertion (RPE) were measured. The tV̇O2max (ITCON: 274±132; ITDEC: 313±102; ITINC: 310±113 s, P=0.37), tHRmax (ITCON: 396±180; ITDEC: 441±207; ITINC: 390±212 s, P=0.47), BLC (P=0.73), and final RPE (P=0.75) were similar among protocols. In conclusion, work-matched interval training induced similar time near V̇O2max and associated physiological responses regardless of work rate manipulation.

Is Bilateral Deficit in Handgrip Strength Associated With Performance in Specific Judo Tasks?

ABSTRACT: Turnes, T, Silva, BA, Kons, RL, and Detanico, D. Is bilateral deficit in handgrip strength associated with performance in specific judo tasks? J Strength Cond Res 36(2): 455-460, 2022-The aim of this study was to identify the existence of bilateral deficit (BD) on maximal handgrip strength during standing and seated positions in judo athletes and to correlate this with judo-specific tasks. Nineteen male judo athletes (age 22.1 ± 4.6 years) performed unilateral and bilateral handgrip strength tests in seated and standing postures to calculate bilateral index (BI). Athletes then performed the Judogi Grip Strength Test (dynamic and isometric modes) and Special Judo Fitness Test. The comparisons between bilateral and unilateral handgrip strength for each position, weight categories, or training experience (novice <10 years and advanced >10 years) were made by paired and unpaired t-tests. The sum of bilateral forces was significantly lower than unilateral forces at standing (unilateral: 105 ± 20 vs. bilateral: 102 ± 21 kgf, BI: -3.0 ± 5.9%, p = 0.043), but not at seated position (unilateral: 100 ± 19 vs. bilateral: 98 ± 19 kgf, BI: -2.1 ± 7.3%, p = 0.230), indicating BD only at standing position. Pearson coefficient correlations were not significant between judo tests and BI, with similar performance in judo tests between athletes with BD and bilateral facilitation (p > 0.05). Significant BD was observed at standing position in advanced (-4.5 ± 7.1%, p = 0.049) but not in novice athletes (-0.9 ± 3.8%, p = 0.592). We concluded that BI in maximal handgrip strength test was not related with judo-specific tests. However, the BD occurred only at standing position, suggesting that postural stability may contribute for this phenomenon.

A-Mode Ultrasound Reliability in Fat and Muscle Thickness Measurement.

ABSTRACT: Ribeiro, G, de Aguiar, RA, Penteado, R, Lisbôa, FD, Raimundo, JAG, Loch, T, Meira, Â, Turnes, T, and Caputo, F. A-mode ultrasound reliability in fat and muscle thickness measurement. J Strength Cond Res 36(6): 1610-1617, 2022-This study aimed to verify the reliability of the BodyMetrix portable A-mode ultrasound in measuring fat and muscle tissue thickness. Thirty physically active men participated in daily body composition evaluations. The evaluations comprised 2 techniques: (a) graphic technique (GTBM), which measured the fat thickness at 9 body sites (abdomen, axillary, biceps brachii, calf, chest, subscapular, suprailiac, thigh, and triceps brachii), and (b) imaging technique (ITBM), which simultaneously measured the fat and muscle thickness of 6 body surfaces (abdomen, biceps brachii, chest, thigh, trapezius, and triceps brachii). Regarding GTBM, relative reliability was moderate to excellent (intraclass correlation coefficient [ICC]: 0.81-0.98), whereas absolute reliability was acceptable for abdomen, calf, chest, subscapular, suprailiac, and triceps brachii (coefficient of variation [CV]: 6.9-8.8%) but high for axillary, biceps brachii, and thigh (CV: 12.0-17.4%) in measuring fat thicknesses. Concerning ITBM, relative reliability was good to excellent (ICC: 0.93-0.99 and 0.90-0.98), whereas absolute reliability was acceptable (CV: 3.0-9.2% and 3.5-5.9%) in measuring fat and muscle thickness, respectively. These findings suggest that the, GTBM was only reliable in measuring fat thickness of abdomen, calf, chest, subscapular, suprailiac, and triceps brachii, whereas ITBM was reliable in measuring both fat and muscle thickness in all regions, but showed better reliability values in measuring muscle than fat thickness.

Effects of Nonperiodized and Linear Periodized Combined Training on Health-Related Physical Fitness in Adults With Obesity: A Randomized Controlled Trial.

ABSTRACT: Streb, AR, Passos da Silva, R, Leonel, LdS, Possamai, LT, Gerage, AM, Turnes, T, and Del Duca, GF. Effects of nonperiodized and linear periodized combined training on health-related physical fitness in adults with obesity: a randomized controlled trial. J Strength Cond Res 36(9): 2628-2634, 2022-The aim of this randomized controlled trial study was to compare the effects of 16 weeks of linear periodized and nonperiodized combined training (CT) in cardiorespiratory fitness, muscle strength, and body composition indicators of adults with obesity. Thirty-four obese adults of both sexes (36.6 ± 4.4 years; body mass index, 32.9 ± 2.7 kg·m -2 ) were divided into nonperiodized (NG; n = 8), linear periodized (PG; n = 11), and control (CG; n = 15) groups. The NG and PG groups performed 3 weekly sessions of CT over 16 weeks in different ways. Anthropometric measures, maximal strength for leg press and barbell bench press, maximal oxygen uptake (V̇ o2 max), and ventilatory thresholds were determined before and after intervention. The generalized estimation equation was used, with the applied level of significance for the interaction of 0.10 and the isolated effect of time or group or both of 0.05. Significant and similar increases were observed in the 1-repetition maximum test for bench press (NG: 48.8 ± 5.7 to 55.0 ± 6.1 kg; PG: 48.7 ± 5.7 to 53.8 ± 5.9 kg; p = 0.001) and leg press (NG: 235.2 ± 18.7 to 268.3 ± 19.7 kg; PG: 223.1 ± 25.3 to 253.3 ± 23.1 kg; p = 0.05) in trained groups. Relative V̇ o2 max improved only in PG (27.8 ± 1.3 to 32.0 ± 1.4 mL·kg·min -1 ; p = 0.05), while ventilatory thresholds improved in NG and CG ( p = 0.004 and p = 0.06). There was an increase in body mass in CG (97.6 ± 3.4 to 99.1 ± 2.9 kg) and NG (92.5 ± 5.4 to 93.5 ± 5.4 kg; p = 0.05). Combined training improved maximal upper-body and lower-body strength, regardless of periodization. However, for improvement in V̇ o2 max, linear periodization may be superior to nonperiodization in obese adults.

Acute Cardiopulmonary, Metabolic, and Neuromuscular Responses to Severe-Intensity Intermittent Exercises.

Lisbôa, FD, Raimundo, JAG, Salvador, AF, Pereira, KL, Turnes, T, Diefenthaeler, F, Oliveira, MFMd, and Caputo, F. Acute cardiopulmonary, metabolic, and neuromuscular responses to severe-intensity intermittent exercises. J Strength Cond Res 33(2): 408-416, 2019-The purpose of this study was to compare cardiopulmonary, neuromuscular, and metabolic responses to severe-intensity intermittent exercises with variable or constant work rate (CWR). Eleven cyclists (28 ± 5 years; 74 ± 7 kg; 175 ± 5 cm; 63 ± 4 ml·kg·min) performed the following tests until exhaustion on separate days: (a) an incremental test; (b) in random order, 2 CWR tests at 95 and 110% of the peak power for the determination of critical power (CP); (c) 2-4 tests for the determination of the highest power that still permits the achievement of maximal oxygen uptake (PHIGH); and (d) 2 random severe-intensity intermittent exercises. The last 2 sessions consisted of a CWR exercise performed at PHIGH or a decreasing work rate (DWR) exercise from PHIGH until 105% of CP. Compared with CWR, DWR presented higher time to exhaustion (635 ± 223 vs. 274 ± 65 seconds), time spent above 95% of V[Combining Dot Above]O2max (t95% V[Combining Dot Above]O2max) (323 ± 227 vs. 98 ± 65 seconds), and O2 consumed (0.97 ± 0.41 vs. 0.41 ± 0.11 L). Electromyography amplitude (root mean square [RMS]) decreased for DWR but increased for CWR during each repetition. However, RMS and V[Combining Dot Above]O2 divided by power output (RMS/PO and V[Combining Dot Above]O2/PO ratio) increased in every repetition for both protocols, but to a higher extent and slope for DWR. These findings suggest that the higher RMS/PO and V[Combining Dot Above]O2/PO ratio in association with the longer exercise duration seemed to have been responsible for the higher t95% V[Combining Dot Above]O2max observed during severe DWR exercise.

Impact of ischaemia-reperfusion cycles during ischaemic preconditioning on 2000-m rowing ergometer performance.

PURPOSE: Although ischaemic preconditioning (IPC), induced by cycles of transient limb ischaemia and reperfusion, seems to improve exercise performance, the optimal duration of ischaemia-reperfusion cycles is not established. The present study investigated the effect of ischaemia-reperfusion duration within each IPC cycle on performance in a 2000-m rowing ergometer test. METHODS: After incremental and familiarization tests, 16 trained rowers (mean ± SD: age, 24 ± 11 years; weight, 74.1 ± 5.9 kg; [Formula: see text] peak, 67.2 ± 7.4 mL·kg-1·min-1) were randomly submitted to a 2000-m rowing test preceded by intermittent bilateral cuff inflation of the lower limbs with three cycles of ischaemia-reperfusion, lasting 5 min (IPC-5) or 10 min (IPC-10) at 220 or 20 mmHg (control). Power output, [Formula: see text], heart rate, blood lactate concentration, pH, ratings of perceived exertion (RPE), and near-infrared spectroscopy-derived measurements of the vastus lateralis muscle were continuously recorded. RESULTS: No differences among treatments were found in the 2000-m test (control: 424 ± 17; IPC-5: 425 ± 16; IPC-10: 424 ± 17 s; P = 0.772). IPC-10 reduced the tissue saturation index and oxy-haemoglobin concentration during exercise compared with control. The power output during the last 100-m segment was significantly lower with IPC-10. The IPC treatments increased the heart rate over the first 500 m and decreased the pH after exercise. No alterations were observed in [Formula: see text], blood lactate, or RPE among the trials. CONCLUSION: In conclusion, IPC does not improve the 2000-m rowing ergometer performance of trained athletes regardless of the length of ischaemia-reperfusion cycles.

Interval training in the boundaries of severe domain: effects on aerobic parameters.

PURPOSE: Although time spent at VO2max (t@VO2max) has been suggested as an optimal stimulus for the promotion of greater VO2max improvements, scientific findings supporting this notion are surprisingly still lacking. To investigate this, the present study described t@VO2max in two different severe-intensity interval training regimens and compared its effects on aerobic indexes after a 4-week intervention. METHODS: Twenty-one recreational cyclists performed an incremental exercise test and six time-to-exhaustion tests on four different days to determine VO2max, lactate threshold (LT), critical power (CP) and the highest intensity (IHIGH) and lowest exercise duration (TLOW) at which VO2max was attained. Subjects were assigned to the lower (LO, n = 11, 4 × 5 min at 105% CP, 1 min recovery) or the upper severe-intensity training groups (UP, n = 10, 8 × 60% TLOW at 100% IHIGH, 1:2 work:recovery ratio). t@VO2max was measured during the first and last training sessions. RESULTS: A significantly higher t@VO2max was elicited in the UP during training sessions in comparison with the LO group (P < 0.05), and superior improvements were observed in VO2max (change in measure ± 95% confidence interval) (6.3 ± 1.9 vs. 3.3 ± 1.8%, P = 0.034 for interaction terms) and LT (54.8 ± 11.8 vs. 27.9 ± 11.3%, P = 0.023 for interaction terms). The other aerobic indexes were similarly improved between the groups. CONCLUSION: The present results demonstrated that UP training produced superior gains in VO2max and LT in comparison with LO training, which may be associated with the higher t@VO2max.

Short-term interval training at both lower and higher intensities in the severe exercise domain result in improvements in V̇O2 on-kinetics.

PURPOSE: Although high-intensity interval training (HIT) seems to promote greater improvements in aerobic parameters than continuous training, the influence of exercise intensity on [Formula: see text] on-kinetics remains under investigation. METHODS: After an incremental test, twenty-one recreationally trained cyclists performed several time-to-exhaustion tests to determine critical power (CP), and the highest intensity (I HIGH), and the lowest exercise duration (T LOW) at which [Formula: see text] is attained during constant exercise. Subjects also completed a series of step transitions to moderate- and heavy-intensity work rates to determine pulmonary [Formula: see text] on-kinetics. Surface electromyography (EMG) of vastus lateralis muscle and blood lactate accumulation (∆BLC) was measured during heavy exercise. Subjects were assigned to one of two 4-week work-matched training groups: the lower [105 % CP: n = 11; 4 × 5 min at 105 % CP (218 ± 39 W), 1 min recovery] or the upper [I HIGH: n = 10; 8 × 100 % I HIGH (355 ± 60 W), 1:2 work:recovery ratio] intensity of the severe exercise domain. RESULTS: The two interventions were similarly effective in reducing the phase II [Formula: see text] time constant during moderate (105 % CP: 34 ± 13 to 25 ± 8 s; I HIGH: 31 ± 9 to 23 ± 6 s) and heavy exercise (105 % CP: 25 ± 7 to 18 ± 5 s; I HIGH: 27 ± 7 to 16 ± 5 s) and in reducing the amplitude of [Formula: see text] slow component, EMG amplitude, and ∆BLC during heavy exercise. CONCLUSION: In conclusion, the short-term adjustments in response to step transitions to moderate and heavy exercise were independent of training intensity within the severe exercise domain.

Fast-start strategy increases the time spent above 95 %VO2max during severe-intensity intermittent running exercise.

This study aimed to use the intermittent critical velocity (ICV) model to individualize intermittent exercise and analyze whether a fast-start strategy could increase the time spent at or above 95 %VO(2max) (t95VO(2max)) during intermittent exercise. After an incremental test, seven active male subjects performed three intermittent exercise tests until exhaustion at 100, 110, and 120 % of the maximal aerobic velocity to determine ICV. On three occasions, the subjects performed an intermittent exercise test until exhaustion at 105 % (IE105) and 125 % (IE125) of ICV, and at a speed that was initially set at 125 %ICV but which then decreased to 105 %ICV (IE125-105). The intermittent exercise consisted of repeated 30-s runs alternated with 15-s passive rest intervals. There was no difference between the predicted and actual Tlim for IE125 (300 ± 72 s and 284 ± 76 s) and IE105 (1,438 ± 423 s and 1,439 ± 518 s), but for IE125-105 the predicted Tlim underestimated the actual Tlim (888 ± 211 s and 1,051 ± 153 s, respectively). The t95VO(2max) during IE125-105 (289 ± 150 s) was significantly higher than IE125 (113 ± 40 s) and IE105 (106 ± 71 s), but no significant differences were found between IE125 and IE105. It can be concluded that predicting Tlim from the ICV model was affected by the fast-start protocol during intermittent exercise. Furthermore, fast-start protocol was able to increase the time spent at or above 95 %VO2max during intermittent exercise above ICV despite a longer total exercise time at IE105.

Prediction of Exercise Tolerance in the Severe and Extreme Intensity Domains by a Critical Power Model.

This study aimed to assess the predictive capability of different critical power (CP) models on cycling exercise tolerance in the severe- and extreme-intensity domains. Nineteen cyclists (age: 23.0 ± 2.7 y) performed several time-to-exhaustion tests (Tlim) to determine CP, finite work above CP (W'), and the highest constant work rate at which maximal oxygen consumption was attained (IHIGH). Hyperbolic power-time, linear power-inverse of time, and work-time models with three predictive trials were used to determine CP and W'. Modeling with two predictive trials of the CP work-time model was also used to determine CP and W'. Actual exercise tolerance of IHIGH and intensity 5% above IHIGH (IHIGH+5%) were compared to those predicted by all CP models. Actual IHIGH (155 ± 30 s) and IHIGH+5% (120 ± 26 s) performances were not different from those predicted by all models with three predictive trials. Modeling with two predictive trials overestimated Tlim at IHIGH+5% (129 ± 33 s; p = 0.04). Bland-Altman plots of IHIGH+5% presented significant heteroscedasticity by all CP predictions, but not for IHIGH. Exercise tolerance in the severe and extreme domains can be predicted by CP derived from three predictive trials. However, this ability is impaired within the extreme domain.

Muscle Oxidative Capacity in Vivo Is Associated With Physiological Parameters in Trained Rowers.

Purpose: The muscle oxygen uptake (mV˙O2) kinetics following exercise, measured by near-infrared spectroscopy, has been used as a functional evaluation of muscle oxidative metabolism. This study aimed to determine the mV˙O2 off-kinetics and verify the relationship of the recovery rate of mV˙O2 (k) with time-trial performance and different aerobic parameters in trained rowers. Methods: Eleven male rowers (age: 20 ± 3 years; V˙O2max: 4.28 ± 0.35 L·min-1) used a rowing ergometer to perform (I) an incremental test to determine the maximal oxygen uptake (V˙O2max) and peak power output (Ppeak); (II) several visits to determine maximal lactate steady state (MLSS); and (III) a 2000-m rowing ergometer performance test. Also, one test to determine mV˙O2 off-kinetics of the vastus lateralis muscle using a repeated arterial occlusions protocol. Results: The mV˙O2 generated a good monoexponential fit (R2 = 0.960 ± 0.030; SEE = 0.041 ± 0.018%.s-1). The k of mV˙O2 (2.06 ± 0.58 min-1) was associated with relative V˙O2max (r = 0.79), power output at MLSS (r = 0.76), and Ppeak (r = 0.83); however, it was not related with 2000-m rowing performance (r = -0.38 to 0.52; p > .152). Conclusion: These findings suggest that although not associated with rowing performance, the mV˙O2 off-kinetics determined after a submaximal isometric knee extension may be a practical and less-exhaustive approach than invasive responses and incremental tests to assess the muscle oxidative metabolism during a training program.

1) The present study showed that the NIRS-derived mV˙O2 off-kinetics from the vastus lateralis can be used as a functional tool of skeletal muscle oxidative metabolism in rowers.2) The results demonstrated that the recovery rate of mV˙O2 was well correlated with important aerobic parameters such as V˙O2max, maximal lactate steady state, and peak power output during an incremental test.

Intracellular shuttle: the lactate aerobic metabolism.

Lactate is a highly dynamic metabolite that can be used as a fuel by several cells of the human body, particularly during physical exercise. Traditionally, it has been believed that the first step of lactate oxidation occurs in cytosol; however, this idea was recently challenged. A new hypothesis has been presented based on the fact that lactate-to-pyruvate conversion cannot occur in cytosol, because the LDH enzyme characteristics and cytosolic environment do not allow the reaction in this way. Instead, the Intracellular Lactate Shuttle hypothesis states that lactate first enters in mitochondria and only then is metabolized. In several tissues of the human body this idea is well accepted but is quite resistant in skeletal muscle. In this paper, we will present not only the studies which are protagonists in this discussion, but the potential mechanism by which this oxidation occurs and also a link between lactate and mitochondrial proliferation. This new perspective brings some implications and comes to change our understanding of the interaction between the energy systems, because the product of one serves as a substrate for the other.

Agreement of maximal lactate steady state with critical power and physiological thresholds in rowing.

The aim of this study was threefold: (a) to compare the maximal lactate steady state (MLSS) with critical power (CP); (b) to describe the relationship of MLSS with rowing performances; and (c) to verify the agreement of MLSS with several exercise intensity thresholds in rowers. Fourteen male rowers (mean [SD]: age = 26 [13] years; height = 1.82 [0.05] m; body mass = 81.0 [7.6] kg) performed on a rowing ergometer: (I) discontinuous incremental test with 3 min stages and 30-s recovery intervals (INC3min); (II) continuous incremental test with 60-s stages (INC1min); (III) two to four constant workload tests to determine MLSS; and (IV) performance tests of 500, 1000, 2000 and 6000 m to determine CP. Twenty-seven exercise intensity thresholds based on blood lactate, heart rate and ventilatory responses were determined by incremental tests, and then compared with MLSS. CP (257 [38] W) was higher than MLSS (187 [25] W; p < 0.001), with a very large mean difference (37%), large typical error of estimate (14%) and moderate correlation (r = 0.48). Despite the correlations between MLSS and most intensity thresholds (r > 0.70), all presented low correspondence (TEE > 5%), with a lower bias found between MLSS and the first intensity thresholds (-12.5% to 4.1%). MLSS was correlated with mean power during 500 m (r = 0.65), 1000 m (r = 0.86) and 2000 m (r = 0.78). In conclusion, MLSS intensity is substantially lower than CP and presented low agreement with 27 incremental-derived thresholds, questioning their use to estimate MLSS during rowing ergometer exercise.Highlights MLSS was substantially lower than CP in rowing exercise with a mean difference of 37%, much larger than the difference commonly found in running and cycling exercise (i.e., ?10%).A clear disagreement was reported between MLSS and 27 physiological thresholds determined in different incremental tests.There is a positive association of MLSS with 500, 1000 and 2000 m rowing ergometer performance tests.

Effects of linear periodization of combined training on quality of life of adults with obesity: a blind randomized controlled trial.

This study aimed to compare the effect of 16-weeks of combining aerobic and strength training with a linear increase or fixed intensity on the health-related quality of life (HRQoL) of obese adults. This single-blinded clinical trial involved adults with obesity (BMI ≥ 30 kg/m2), randomized into control (CG), fixed intensity (FG), or linear increase (LG) groups. The FG and LG performed 16 weeks of combined (aerobic + strength) training for 60 min, three times a week. The FG performed aerobic exercises between 50 and 59% of the heart rate reserve (HRres) and strength at 10-12 maximum repetitions (RM). The LG started with 40-49% of HRres and 12-14 RM and progressively increased the intensity (50-59% and 10-12 RM; 60-69% and 8-10 RM). The HRQoL was assessed using the SF-36 questionnaire. Generalized estimation equations and mean differences (∆) were used. Of the 69 participants (23 per group), 36 completed the intervention (CG = 13, FG = 9, and LG = 14). A significant difference was observed in the time of the physical function, with superiority in the training groups (CG: ∆ = 1.2 vs. FG and LG, respectively: ∆ = 10.0). The mental health component and mental health domain showed significant differences for the FG (∆ = 30.2 and ∆ = 23.1, respectively). In conclusion, the combined training improved physical functioning. Specifically, fixed-intensity training effectively enhanced mental health indicators.Trial Registration: This study is registered at www.ensaiosclinicos.gov.br/ (No. RBR-3c7rt3), Date of registration: 07/02/2018.

Time Spent Near V˙O2max During Different Cycling Self-Paced Interval Training Protocols.

PURPOSE: Cyclists may increase exercise intensity by prolonging exercise duration and/or shortening the recovery period during self-paced interval training, which could impact the time spent near V˙O2max. Thus, the main objective of this study was to compare the time spent near V˙O2max during 4 different self-paced interval training sessions. METHODS: After an incremental test, 11 cyclists (mean [SD]: age = 34.4 [6.2] y; V˙O2max=55.7 [7.4] mL·kg-1·min-1) performed in a randomized order 4 self-paced interval training sessions characterized by a work-recovery ratio of 4:1 or 2:1. Sessions comprised 4 repetitions of 4 minutes of cycling with 1 minute (4/1) or 2 minutes (4/2) of active recovery or 8 minutes of cycling with 2 minutes (8/2) or 4 minutes (8/4) of active recovery. Time spent at 90% to 94% (t90V˙O2max), ≥95% (t95V˙O2max), and 90% to 100% V˙O2max (tV˙O2max) was analyzed in absolute terms and relative to the total work duration. Power output, heart rate, blood lactate, and rating of perceived exertion were compared. RESULTS: The 8/4 session provided higher absolute tV˙O2max and t95V˙O2max than 8/2 (P = .015 and .029) and 4/1 (P = .002 and .047). The 4/2 protocol elicited higher relative tV˙O2max (47.7% [26.9%]) and t95V˙O2max (23.5% [22.7%]) than 4/1 (P = .015 and .028) and 8/2 (P < .01). Session 4/2 (275 [23] W) elicited greater mean power output (P < .01) than 4/1 (261 [27] W), 8/4 (250 [25] W), and 8/2 (234 [23] W). CONCLUSIONS: Self-paced interval training composed of 4-minute and 8-minute work periods efficiently elicit tV˙O2max, but protocols with a work-recovery ratio of 2:1 (ie, 4/2 and 8/4) could be prioritized to maximize tV˙O2max.

Mechanical power during an incremental test can be estimated from 2000-m rowing ergometer performance.

BACKGROUND: This study aimed to identify the relationship between the mean power output of 2000-m rowing ergometer performance with the peak power output obtained during an incremental test, in addition to verifying the possibility of using the 2000-m mean power as an intensity associated with V̇O2max. METHODS: Nineteen rowers (age 25.5±10.6 years; V̇O2peak: 65.4±5.8 mL/kg/min) completed an incremental test followed by a 2000-m familiarization test (day 1) and a 2000-m rowing ergometer test (day 2). During the incremental test, the peak power output (PV̇O2peak) and the exercise intensity thresholds were determined. The maximal oxygen uptake (V̇O2peak) was analyzed in both tests. RESULTS: The PV̇O2peak (284.8±44.7 W) presented the highest correlation coefficient (r=0.978) with 2000-m mean power (284.2±49.9 W) and was the only one included in stepwise regression explaining 96% of 2000-m mean power. V̇O2peak was lower in the incremental test (4.69±0.61 L/min) compared with the 2000-m rowing ergometer test (4.86±0.63 L/min; P=0.01) with a small bias limits of agreement (0.16±0.53 L/min). CONCLUSIONS: 2000-m mean power is a valid tool to estimate the PV̇O2peak and allow the achievement of V̇O2peak. Furthermore, the PV̇O2peak was the most important predictor of performance.

Similar maximal oxygen uptake assessment from a step cycling incremental test and verification tests on the same or different day.

The present study aimed to compare maximal oxygen uptake of a step incremental test with time to exhaustion verification tests (TLIM) performed on the same or different day. Nineteen recreationally trained cyclists (age: 23 ± 2.7 years; maximal oxygen uptake: 48.0 ± 5.8 mL·kg-1·min-1) performed 3 maximal tests as follows: (i) same day: an incremental test with 3-min stages followed by a TLIM at 100% of peak power output of the incremental test (TLIM-SAME) interspaced by 15 min; and (ii) different day: a TLIM at 100% of peak power output of the incremental test (TLIM-DIFF). The maximal oxygen uptake was determined for the 3 tests. The maximal oxygen uptake was not different among the tests (incremental: 3.83 ± 0.41; TLIM-SAME: 3.72 ± 0.42; TLIM-DIFF: 3.75 ± 0.41 L·min-1; P = 0.951). Seven subjects presented a variability greater than ±3% in both verification tests compared with the incremental test. The same-day verification test decreased the exercise tolerance (240 ± 38 vs. 310 ± 36 s) compared with TLIM-DIFF (P < 0.05). In conclusion, the incremental protocol is capable of measuring maximal oxygen uptake because similar values were observed in comparison with verification tests. Although the need for the verification phase is questionable, the additional tests are useful to evaluate individual variability. Novelty Step incremental test is capable of measuring maximal oxygen uptake with similar values during TLIM on the same or different day. Although the necessity of the verification phase is questionable, it can allow the determination of variability in maximal oxygen uptake.

Comparative effects of two heat acclimation protocols consisting of high-intensity interval training in the heat on aerobic performance and thermoregulatory responses in exercising rats.

Acclimation resulting from low- to moderate-intensity physical exertion in the heat induces several thermoregulatory adaptations, including slower exercise-induced increases in core body temperature. However, few studies have investigated the thermoregulatory adaptations induced by high-intensity interval training (HIIT) protocols. Thus, the present study aimed to compare the adaptations in rats' thermoregulatory parameters and aerobic performance observed after two different heat acclimation regimens consisting of HIIT protocols performed in a hot environment. Twenty-three adult male Wistar rats were initially subjected to an incremental-speed exercise at 32°C until they were fatigued and then randomly assigned to one of the following three heat acclimation strategies: passive heat exposure without any exercise (untrained controls-UN; n = 7), HIIT performed at the maximal aerobic speed (HIIT100%; n = 8) and HIIT performed at a high but submaximal speed (HIIT85%; n = 8). Following the two weeks of interventions, the rats were again subjected to a fatiguing incremental exercise at 32°C, while their colonic temperature (TCOL) was recorded. The workload performed by the rats and their thermoregulatory efficiency were calculated. After the intervention period, rats subjected to both HIIT protocols attained greater workloads (HIIT100%: 313.7 ± 21.9 J vs. HIIT85%: 318.1 ± 32.6 J vs. UN: 250.8 ± 32.4 J; p < 0.01) and presented a lower ratio between the change in TCOL and the distance travelled (HIIT100%: 4.95 ± 0.42°C/km vs. HIIT85%: 4.33 ± 0.59°C/km vs. UN: 6.14 ± 1.03°C/km; p < 0.001) when compared to UN rats. The latter finding indicates better thermoregulatory efficiency in trained animals. No differences were observed between rats subjected to the two HIIT regimens. In conclusion, the two HIIT protocols induce greater thermoregulatory adaptations and performance improvements than passive heat exposure. These adaptations do not differ between the two training protocols investigated in the present study.