Myc-binding protein orthologue interacts with AKAP240 in the central pair apparatus of the Chlamydomonas flagella.

BACKGROUND: Flagella and cilia are fine thread-like organelles protruding from cells that harbour them. The typical '9 + 2' cilia confer motility on these cells. Although the mechanistic details of motility remain elusive, the dynein-driven motility is regulated by various kinases and phosphatases. A-kinase anchoring proteins (AKAPs) are scaffolds that bind to a variety of such proteins. Usually, they are known to possess a dedicated domain that in vitro interacts with the regulatory subunits (RI and RII) present in the cAMP-dependent protein kinase (PKA) holoenzyme. These subunits conventionally harbour contiguous stretches of a.a. residues that reveal the presence of the Dimerization Docking (D/D) domain, Catalytic interface domain and cAMP-Binding domain. The Chlamydomonas reinhardtii flagella harbour two AKAPs; viz., the radial spoke AKAP97 or RSP3 and the central pair AKAP240. Both these were identified on the basis of their RII-binding property. Interestingly, AKAP97 binds in vivo to two RII-like proteins (RSP7 and RSP11) that contain only the D/D domain. RESULTS: We found a Chlamydomonas Flagellar Associated Protein (FAP174) orthologous to MYCBP-1, a protein that binds to organellar AKAPs and Myc onco-protein. An in silico analysis shows that the N-terminus of FAP174 is similar to those RII domain-containing proteins that have binding affinities to AKAPs. Binding of FAP174 was tested with the AKAP97/RSP3 using in vitro pull down assays; however, this binding was rather poor with AKAP97/RSP3. Antibodies were generated against FAP174 and the cellular localization was studied using Western blotting and immunoflourescence in wild type and various flagella mutants. We show that FAP174 localises to the central pair of the axoneme. Using overlay assays we show that FAP174 binds AKAP240 previously identified in the C2 portion of the central pair apparatus. CONCLUSION: It appears that the flagella of Chlamydomonas reinhardtii contain proteins that bind to AKAPs and except for the D/D domain, lack the conventional a.a. stretches of PKA regulatory subunits (RSP7 and RSP11). We add FAP174 to this growing list.

Prevalence and pattern of proximity of maxillary posterior teeth to maxillary sinus with mucosal thickening: A cone beam computed tomography based retrospective study.

Context: Odontogenic sinusitis is a prevalent but frequently unrecognized condition, and periapical pathologies of maxillary posterior teeth are accused as one of the main causative factors. Aim: This study aimed to evaluate the relationship between the periapical status of maxillary posterior teeth and its proximity to the maxillary sinus floor in the occurrence of incidental sinus pathologies using cone-beam computed tomography (CBCT). Methodology: CBCT scans of 118 patients of age range 18-77 years were evaluated retrospectively to determine the relationship of maxillary posterior teeth to sinus floor in which vertical relationship was assessed using modified Kwak's classification and periapical status using CBCT periapical index. Statistical analysis was performed using SPSS statistics software. Results: Of all 227 sinuses examined, 56.8% showed pathological changes, with mucosal thickening being the most common. More than 50% (50.2%) of sinuses were associated with periapical lesions of at least one maxillary posterior tooth based on evidence of pathological mucosal thickening. The presence of pathologic mucosal thickening was also significantly related (P < 0.05) to the presence of periapical pathologies. There was a significant association between tooth position and pathological sinus mucosal thickening, especially with second molars, first molars, and second premolars, respectively (P < 0.05). Second molar involvement was the most significant (P < 0.05). Conclusion: The present study established a positive association between periapical disease status of maxillary posteriors and maxillary sinus mucosal thickening. Maxillary second premolar and first and second molars pathologies can significantly affect the maxillary sinus compared to other maxillary posterior tooth. CBCT proved to be an efficient imaging modality in detecting these changes.

Résumé Contexte: La sinusite odontogène est une affection fréquente mais souvent méconnue, et les pathologies périapicales des dents postérieures maxillaires sont accusés comme l'un des principaux facteurs causals. Objectif: Cette étude visait à évaluer la relation entre le statut périapical du maxillaire dents postérieures et sa proximité avec le plancher du sinus maxillaire dans la survenue de pathologies sinusales accidentelles à l'aide du calcul par faisceau conique tomographie (CBCT). Méthodologie: Les scans CBCT de 118 patients âgés de 18 à 77 ans ont été évalués rétrospectivement pour déterminer la relation entre les dents postérieures maxillaires et le plancher sinusal dans laquelle la relation verticale a été évaluée à l'aide de la classification de Kwak modifiée et état périapical à l'aide de l'index périapical CBCT. L'analyse statistique a été réalisée à l'aide du logiciel de statistiques SPSS. Résultats: Sur les 227 sinus examinés, 56.8 % présentaient des modifications pathologiques, l'épaississement de la muqueuse étant le plus fréquent. Plus de 50 % (50.2 %) des sinus étaient associée à des lésions périapicales d'au moins une dent postérieure maxillaire sur la base de preuves d'un épaississement pathologique de la muqueuse. La presence d'épaississement muqueux pathologique était également significativement lié (P < 0.05) à la présence de pathologies périapicales. Il y avait un important association entre la position des dents et l'épaississement pathologique de la muqueuse des sinus, en particulier avec les deuxièmes molaires, les premières molaires et les deuxièmes prémolaires, respectivement (P < 0.05). L'atteinte de la deuxième molaire était la plus importante (P < 0.05). Conclusion: La présente étude a établi un bilan positif association entre l'état de la maladie périapicale des postérieurs maxillaires et l'épaississement de la muqueuse du sinus maxillaire. Deuxième prémolaire maxillaire et les pathologies des premières et deuxièmes molaires peuvent affecter de manière significative le sinus maxillaire par rapport aux autres dents postérieures maxillaires. Le CBCT s'est avéré être une modalité d'imagerie efficace pour détecter ces changements. Mots-clés: Tomodensitométrie à faisceau conique, épaississement de la muqueuse du sinus maxillaire, sinusite odontogène, pathologie périapicale.

Migratory behaviour of Brown planthopper, Nilaparvata lugens (Stål) (Hemiptera: Delphacidae), in India as inferred from genetic diversity and reverse trajectory analysis.

The brown planthopper, Nilaparvata lugens (Stål) is a major sucking insect pest of rice. This insect has long been considered as migratory; however, its route in India is still unknown. Hence, to find out its migration route genetic diversity, genetic structure and gene flow of 16 N. lugens populations from major rice growing regions of India was studied based on mitochondrial cytochrome oxidase I (COI). The results revealed a high genetic homogeneity among the populations on the basis of genetic diversity statistics and neutrality tests. There was a prevalence of a single major haplotype across the country. No spatial relevance was found with the genetic structure of the populations indicating presence of excessive gene flow among them. Extensive gene flow among populations was also confirmed with the presence of higher number of immigrants in North, Central, and East India. To further clarify the migration sources, 48 h air-mass reverse trajectory was performed for Varanasi just aftermath of cyclones Amphan and Yaas, which disclosed Eastern/Northeastern states along with Bangladesh and Myanmar as the possible source areas. Overall, the results revealed a single panmictic homogeneous population of N. lugens in India with extensive gene flow as a consequence of their migration. These findings will help in better forecasting enabling efficient regional management of this important rice pest. Supplementary Information: The online version contains supplementary material available at 10.1007/s13205-022-03337-6.

Actual applications of magnetic resonance imaging in dentomaxillofacial region.

Magnetic resonance imaging (MRI) is a versatile imaging modality utilized in various medical fields. Specifically used for evaluation of soft tissues, with non-ionizing radiation and multiplanar sections that has provided great guidance to diagnosis. Nowadays, use of MRI in dental practice is becoming more pervasive, especially for the evaluation of head-and-neck cancer, detection of salivary gland lesions, lymphadenopathy, and temporomandibular joint disorders. Understanding the basic principles, its recent advances, and multiple applications in dentomaxillofacial region helps significantly in the diagnostic decision making. In this article, the principle of MRI and its recent advances are reviewed, with further discussion on the appearance of various maxillofacial pathosis.

A Rare Case Of Shprintzen-Goldberg Syndrome.

Shprintzen-Goldberg syndrome is a relatively rare congenital connective tissue type of disorder with a constellation of dysmorphic features including craniosynostosis, craniofacial, skeletal, cardiovascular and neurological abnormalities. We present the case-report of a 5-year-old boy with Shprintzen-Goldberg syndrome and a brief review of literature pertaining to this condition. The patients with Shprintzen-Goldberg syndrome show a considerable phenotypic overlap with other craniosynostosis syndromes. So, a meticulous evaluation of these patients should be performed for a prudent diagnosis. Since these patients present with multiple systemic conditions,a multidisciplinary approach should be planned for their management.

Knowledge and attitude toward COVID-19 and dental treatment - Its availability and treatment satisfaction during the pandemic among adult population - An online survey.

BACKGROUND: Corona virus disease 2019 (COVID-19) is a highly infectious disease primarily spreading through droplet infection which require significant restriction and modification in dental treatment. This study aims to assess the knowledge and attitude of adult population toward COVID-19 and dental treatment and to assess the availability of dental treatment and patient satisfaction during the time of pandemic. MATERIALS AND METHODS: An online survey was conducted using self-designed electronic questionnaire consisting knowledge and attitude about COVID-19 and dental scenario and questions regarding availability of dental treatment received during the time of pandemic. Knowledge scores were assessed on a maximum total score ranged from 0 to 8, and descriptive statistics were done for questions related to attitudes and dental treatment availability. RESULTS: A total of 495 participants with mean age of 36.6 years participated in the study. The mean knowledge score was 7.12 ± 0.99 and the participants were found to have good awareness and positive attitude regarding COVID-19 were as considerable number of participants were unaware about the risk associated with dental treatment as well as restrictions imposed on dental procedures. About 18% of participants experienced one or other form of dental complaints during the lockdown period. 4% of participants had no accessibility to any form of treatment. CONCLUSIONS: Public awareness is to be improved regarding risk of cross infection that can be associated with dental treatment and public should be motivated to use virtual facilities like tele-dentistry so that no dental emergencies is left untreated, at the same time avoiding any sort of unnecessary hospital visit during the pandemic time.

Craniofacial Langerhans Cell Histiocytosis: A Rare Case Report.

Langerhans cell histiocytosis (LCH) is a rare haematological disorder characterised by accumulation and abnormal proliferation of bone marrow-derived Langerhans cells and mature eosinophils. The clinical presentation of patients with LCH may be localised or systemic, depending on the site and the degree of involvement. LCH may involve any bone, but the most commonly involved bones are long bones, pelvis, ribs, skull, vertebrae, and facial bones. In head and neck region, frontal and parietal bones are commonly involved followed by the jaws, with a predilection for mandible. In this report, we present a case of LCH involving the craniofacial bones in a 10-year boy, who presented initially with multiple ulcerative lesions on gingiva. Key Words: Craniofacial, Langerhans cell histiocytosis, Gingiva.

Radiographic evaluation of remodeling of mandible in adult South Indian population: Implications in forensic science.

INTRODUCTION: Panoramic X-ray is an imagiological examination produced by a technique that exhibits images of the facial structure present in the upper and lower dental arches, as well as the support structures in the diagnosis of problems that require a broad view of the oral-maxillofacial complex, such as the assessment of traumatisms, extensive lesions, dental development, tooth retention, and growth anomalies. Many morphological and anatomical changes are exhibited by the mandible with the advancement of age and changes in gender, as well as dentoalveolar condition of the patient, and it can be assessed with the help of such radiograph, thus playing an important role in personal identification in forensic dentistry. AIMS: This study aimed to evaluate the changes in gonial angle (GA), antegonial angle (AGA), and antegonial depth (AGD) in adult mandible with regard to age, gender, and dental status using panoramic radiographs. SETTINGS AND DESIGN: This cross-sectional retrospective study was done using hospital records. SUBJECTS AND METHODS: A total of 100 panoramic radiographs were evaluated. The images were grouped into three age groups (40-70) of 10 years each. GA, AGA, and AGD were digitally measured from the radiographs. STATISTICAL ANALYSIS USED: Descriptive statistics of the variables were expressed in percentage, frequencies, mean, and standard deviations. Statistical differences between the variables were explored using independent sample t-test and one-way ANOVA. The significant level was set as 0.05. RESULTS: There were no significant changes in GA, AGA, and AGD with age and dental status of the patients. Males had significantly smaller GA and AGA than females. Understandably, AGD was significantly greater in males than females. CONCLUSIONS: Remodeling changes of the mandible with respect to the age groups and dental status studied were minimal. However, gender-related differences in ante AGA, depth, and GA were significant, which can be used as a tool in forensic identification.

Genetic homogeneity in South American tomato pinworm, Tuta absoluta: a new invasive pest to oriental region.

South American tomato leaf miner, Tuta absoluta (Meyrick, 1917) (Lepidoptera: Gelechiidae), is an important invasive pest of Tomato which invaded India and Nepal in 2014 and 2016, respectively. In the present study, samples from five localities of India and one from Nepal were used for the investigation of genetic diversity of T. absoluta by employing a fragment in the mtDNA gene-encoding cytochrome oxidase I (COI). Based on the partial COI gene, high genetic homogeneity was detected in T. absoluta populations of India and Nepal with rest of the world. Less nucleotide diversity (π 0.00137) was also detected in the populations of T. absoluta from different countries. This is first attempt to analyze molecular data for this new invasive species from India and Nepal.

Comparative evaluation of antidiabetic potential of partially purified bioactive fractions from four medicinal plants in alloxan-induced diabetic rats.

BACKGROUND: Syzygiumcumini, Terminaliachebula, Trigonellafoenumgraecum and Salvadora persica are medicinally important plants well known for their pharmacological activities. AIM: The aim of this study is to compare the antidiabetic potential of partially purified bioactive fractions isolated from four targeted medicinal plants in diabetic rats. METHODS: Alloxan was administered (125 mg/kg, IP) in albino Wistar rats to produce diabetes. The partially purified bioactive fractions, namely S. cumini tannin fraction (ScTF), T. foenumgraecum (Fenugreek) saponin fraction (FgSF), T. chebula flavonoid fraction (TcFF) and S. persica flavonoid fraction (SpFF), were administered to diabetic rats with the dose of 100 mg/kg, per oral (PO) and the effect of the fractions on body weight, liver glycogen and serum glucose were studied up to 15 days. RESULTS: The results have indicated that diabetic rats treated with fractions showed a statistically significant (P < 0.05) decrease in serum glucose and increase in body weight and liver glycogen. Among ScTF, FgSF, TcFF and SpFF possesse better hypoglycemic activity in all models. CONCLUSION: The present investigation reveals that flavonoid isolated from S. persica is useful in the management of diabetes mellitus because of ability to regulate glucose level and reduce related complications.

Cell death does not herald epithelial involution ("atrophy") in oral sub mucous fibrosis: a TEM study.

The disease progression in oral submucous fibrosis (OSF) seems to be in a biphasic manner, along both fibroblastic and keratinocytic lineages. The epithelial malignancy is considered to be a sequel of connective tissue changes. "Atrophy" of epithelium makes it amenable to the effect of oral carcinogens. This concept looks rather simplistic in the light of the current understanding of epithelial cell biology. So the concept of epithelial "atrophy" needs redressal against the backdrop of recent investigations. 12 cases of clinically and histologically advanced OSF cases (M:F = 4:8) who were habitual areca-quid chewers comprise the study group. 5 (M:F=1:4) non-OSF, non-areca-nut chewing healthy volunteers, constituted the control group. Biopsy was done and the sections were processed for light and electron microscopy. Cell countings were made based on established criteria for apoptosis and necrosis under the high resolution of a TEM and electron micrographs were taken. The Apoptotic Index (A1) calculated for the diseased mucosa was 3.0 +/- 1.3 and for the control was 2.1 +/- 1.5 (X2 = 1.21, df= 1, p>0.05). The necrotic indices (NI) were respectively, 2.5 +/- 0.6 and 2.0 +/- 1.3 (X2 = 0.24, df = 1, p>0.05). The Absolute Cell death Index (ACI), which is the cumulative figure of apoptosis and necrosis indices, was 5.5 and 4.2 respectively for diseased and normal samples (X2 = 1.8, df = 1, p>0.05). The inability to compute an increased ACI in OSF epithelium, when compared to normal, goes against the concept of epithelial "atrophy". Atrophy envisages an increased apoptotic cell death of keratinocytes, induced by the same signals that caused atrophy and this may contribute to loss of cell of an entire organ. This basic concept in pathology seems to be unfounded with disease. This prompted us for an alternative concept in favour of a reduced proliferation index of the adult stem cell compartment of the oral epithelium. So this study favours the concept of epithelial hypoplasia, rather than atrophy, which causes thinning of surface epithelium in advanced OSF.