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Sustainable effect of a nitrogen-fixing bacterium Sinorhizobium meliloti on nodulation and photosynthetic traits (phenomenological fluxes) in four leguminous plants species under low moisture stress (20-25% soil moisture content) environment was studied. Sinorhizobium meliloti was isolated from fenugreek (Trigonella foenum-graecum) root nodules, and later, it was cultured and purified. Nodulation and photosynthetic ability in the presence of S. meliloti were tested in four leguminous plant species, that is, kidney bean (cv. lobia-2000), black bean (cv. NM-97), mung bean (cv. NM-2006) and chickpea (cv. Pb-2008). Plants of each species were grown in sterilized soil that was previously treated with 25 ml suspension containing S. meliloti at 41 × 106 CFU ml-1 kg-1 pot. One-month-old plants were subjected to low soil moisture stress conditions for 15 days, and soil moisture contents were maintained to 20-25% throughout the experimental period. The ability to fix nitrogen, nodule formation, and their subsequent effect on phenomenological fluxes in low moisture treated legumes were studied.
Assuntos
Cicer/microbiologia , Medicago sativa/microbiologia , Phaseolus/microbiologia , Nodulação/fisiologia , Sinorhizobium meliloti/metabolismo , Vigna/microbiologia , Nitrogênio/metabolismo , Fixação de Nitrogênio/fisiologia , Fenótipo , Fotossíntese/fisiologia , Solo , Estresse Fisiológico , SimbioseRESUMO
Background: Mathematical modeling provides grounds for understanding scientific systems theoretically. It serves as a guide for experimentalists in determining directions of investigation. Recently, the Covid-19 pandemic has caused disturbances in almost every walk of life. Scientists have played their role and have continued research on the effects of the pandemic. Various mathematical models have been used in different branches of science (Djilali et al. in Phys Scr 96 12 124016, 2021; Math Biosci Eng 18(6):8245-8256, 2021; Zeb et al. in Alex Eng J 61(7):5649-5665). Well-established mathematical models give results close to those obtained by experiments. The Weakest Bound Electron Potential Model is one such model, which explains hydrogen-like atoms and ions. This model has been used extensively for hydrogen-like atoms and ions to calculate energies of Rydberg levels and ionization energies. This model has been used extensively for hydrogen-like atoms and ions to calculate energies of Rydberg levels and ionization energies. Results: This paper presents the energies of the Rydberg series, 2s2ns, and 2s2np of Li I, calculated using WBEPM. The energies are used to calculate transition probabilities from np to 2s, 3s, 4s, and 5s levels. The transition probabilities are compared with corresponding values in published data where available. The agreement with known values is good; most of the transition probabilities calculated in this work are new. A computer program was developed to find the value of the dipole matrix element. The calculations were further verified by calculating the lifetimes of some low-lying levels. Conclusions: Four series of Li I have been studied, and energies of the Rydberg levels in the series were calculated. The energies then are used to calculate transition probabilities from np to ms transitions, where m = 2, 3, 4, & 5 and n = 1-15. The results are compared where available. An excellent agreement with previously published data shows the reliability of calculations. Most of the transition probabilities are new.
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It is well known that egg storage reduces embryo performance, but the fundamental reasons for reduced embryo quality remain unclear. The objective of this study was to investigate possible cellular and molecular mechanisms that might reduce embryo quality after egg storage. Broiler hatching eggs were obtained from the Ross 308 broiler strain, divided into 2 groups, and stored (4 and 14 d) under the same temperature and humidity conditions. Samples of the eggs were used to assess embryo quality by determining daily embryo weight (wet and dry) from 4 to 21 d of incubation. To understand possible cellular and molecular mechanisms that might affect embryo quality, blastoderms (unincubated embryos) were isolated from a sample of eggs from each storage group, dissociated into single cells, and subjected to flow cytometry analysis to differentiate between viable, apoptotic, and necrotic cell populations. Quantitative real-time PCR analysis was used to compare the expression of selected apoptotic genes (Bcl-2 homologous antagonist/killer gene, Bcl-2-associated X gene, Bcl-2-related ovarian killer gene, B-cell lymphoma 2 gene, and B-cell lymphoma xL gene) in blastoderms and embryos (6 d old after incubation). Data were analyzed by the MIXED model procedure of SAS (SAS Institute, Cary, NC), with significance set at P ≤ 0.05. After covariance analysis of initial egg weights, the results showed decreased daily embryo weights (wet and dry), an indication of decreased embryo quality that could affect hatch quality. In addition, a decrease in blastodermal cell viability was associated with an increased percentage of apoptotic cell deaths (P < 0.0001). Expression of pro-apoptotic genes (Bcl-2 homologous antagonist/killer gene, Bcl-2-associated X gene, and Bcl-2-related ovarian killer gene) were upregulated at the blastodermal level as the storage duration increased, but all genes were downregulated after 6 d of incubation. This suggests that an increase in egg storage duration could activate mechanisms of apoptotic cell death at the blastodermal level, which may be one of the molecular mechanisms that leads to reduced daily embryonic weight during incubation. Experimental controls capable of reducing the cellular and molecular mechanisms of egg storage should be used to increase embryo quality.
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Embrião de Galinha/fisiologia , Galinhas/fisiologia , Óvulo/fisiologia , Animais , Blastoderma/citologia , Morte Celular , Sobrevivência Celular , Embrião de Galinha/citologia , Regulação da Expressão Gênica no Desenvolvimento/fisiologia , Óvulo/citologia , RNA/metabolismo , Fatores de TempoRESUMO
It was previously reported that peripheral blood mononuclear cells (PBMC) from the patients with psoriasis vulgaris (PV) showed a reduced proliferative response in vitro to the stimulation of a lyophilized preparation of penicillin-treated low virulence Su-strain of Streptococcus pyogenes group 3, OK-432. In this study, at first it was examined whether OK-432 acts as a superantigen. By analyzing the usage of Vbeta T-cell receptor (TCR) of proliferating T cells stimulated with OK-432, it was found that OK-432 stimulated preferentially Vbeta2 TCR-bearing T cells. Next, to find differences in in vitro responses of PBMC among various types of sterile pustular dermatoses such as pustulosis palmaris et plantaris (PPP), acrodermatitis continua of Hallopeau (AC), and generalized pustular psoriasis (GPP), the proliferative responses of PBMC obtained from these patients under the stimulation of OK-432 were compared. When the PBMC was stimulated with interleukin (IL)-2, no significant difference was found in their proliferative responses among those obtained from the patients with these sterile pustular dermatoses, PV or healthy controls. However, like those from PV patients, PBMC from AC and GPP patients showed significantly smaller responses to OK-432 than those from the healthy controls. In contrast, the proliferative responses of PBMC from the patients with PPP to OK-432 was comparable to those from healthy controls. These results, in addition to its unique clinical and histopathological characteristics, suggest that PPP has a different pathogenetic background from that underlying PV, AC, or GPP.
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Acrodermatite/sangue , Monócitos/efeitos dos fármacos , Monócitos/patologia , Picibanil/farmacologia , Psoríase/sangue , Divisão Celular/efeitos dos fármacos , Células Cultivadas , Humanos , Pessoa de Meia-Idade , Receptores de Antígenos de Linfócitos T alfa-beta/fisiologia , Valores de Referência , Linfócitos T/efeitos dos fármacos , Linfócitos T/fisiologiaRESUMO
This article describes the sensitivity and specificity of troponin I when compared to creatine kinase-MB (CK-MB) and electrocardiography (ECG) for diagnosing acute myocardial infarction (AMI). Two different lower levels for defining positive results with troponin I were evaluated. A retrospective study of 153 patients who presented to the emergency department of a community hospital supplied the pool of patients for this study. Patients included in this study were those for whom a CK-MB was ordered. The majority of these patients were evaluated for chest pain or symptoms suggesting an acute cardiac event. Of the 153 patients studied, CK-MB results were positive in 91 (59%) patients; ECG revealed AMI in 72 (47%) patients. There were 103 (67%) patients who had either positive CK-MB or ECG results. Ninety (59%) patients had a troponin I level greater than 2.0 ng/mL, and 18 (12%) patients had a troponin I level between 0.6 and 2.0 ng/mL. Seven patients whose troponin I level was between 0.6 and 2.0 ng/mL had negative CK-MB and ECG results. Therefore, 11 patients with troponin I between 0.6 and 2.0 ng/mL had AMI. Five patients with positive troponin I results (> 2.0 ng/mL) had negative CK-MB and ECG results. When a troponin I level greater than 0.6 ng/mL was used as a positive value, compared to CK-MB and ECG using either time zero or time 6 hours, the sensitivity was 94% and specificity was 81%. When troponin I greater than 2.0 ng/mL was used to define a positive test, the sensitivity was 85% and specificity was 91% when compared to CK-MB and ECG.
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Creatina Quinase/sangue , Eletrocardiografia , Infarto do Miocárdio/diagnóstico , Troponina I/sangue , Adulto , Idoso , Estudos de Avaliação como Assunto , Feminino , Humanos , Masculino , Pessoa de Meia-Idade , Infarto do Miocárdio/sangue , Estudos Retrospectivos , Sensibilidade e EspecificidadeRESUMO
With rising public expectations and demands for increased accountability, hospitals will be even more challenged by their local communities to implement strategies such as those developed at SMH to enable community participation that assists the hospital in serving its local population into the future. In response to unique health needs of its local community, SMH created an Inner City Health (ICH) Program in the early 1990s to provide comprehensive inpatient, ambulatory, and community outreach services. To build on its philosophy to reduce barriers to health for disadvantaged populations, the ICH Research Program, the only one of its kind in Canada, was also established. This paper will describe and explore the formalised infrastructure between SMH and the community; its evolution, as well as SMH's involvement with its community in context of the hospital's relationships with its Board of Directors, the Toronto teaching hospital system, media and advocacy groups. The role of ICH research in influencing evidence-based clinical practice, innovations in health science education and government policy will also be discussed as part of the SMH's hospital-community infrastructure.
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Relações Comunidade-Instituição , Hospitais Comunitários/organização & administração , Serviços Urbanos de Saúde/organização & administração , CanadáRESUMO
This brief report explores the direction being pursued by hospitals interested in outsourcing non-core activities within the pharmacy department. Private sector logistics companies are looking to position themselves in the drug product supply chain to facilitate seamless transfers of drug products, ordering information and payments between drug manufacturers and hospitals. Opportunities for implementing consolidated purchasing, unit dosing, just-in-time inventory and electronic commerce systems are discussed.
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Serviços Contratados/organização & administração , Sistemas de Medicação no Hospital/organização & administração , Serviço de Farmácia Hospitalar/organização & administração , Comércio , Redes de Comunicação de Computadores , Eficiência Organizacional , Relações Interinstitucionais , Ontário , Sistemas Automatizados de Assistência Junto ao Leito , Setor Privado , Setor Público , Serviço Hospitalar de ComprasRESUMO
Health services restructuring demands that hospitals consider innovative ways of providing patient care, including services within the clinical support departments such as medical imaging, diagnostic laboratories and pharmacy. This Brief Report describes the result of work carried out a St. Michael's Hospital to explore options for public-private management partnerships within the Department of Medical Imaging. Various partnership models and opportunities for cost containment, revenue generation and strategic positioning are discussed.