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1.
Nat Immunol ; 9(8): 908-16, 2008 Aug.
Artigo em Inglês | MEDLINE | ID: mdl-18604211

RESUMO

Autophagy, an evolutionally conserved homeostatic process for catabolizing cytoplasmic components, has been linked to the elimination of intracellular pathogens during mammalian innate immune responses. However, the mechanisms underlying cytoplasmic infection-induced autophagy and the function of autophagy in host survival after infection with intracellular pathogens remain unknown. Here we report that in drosophila, recognition of diaminopimelic acid-type peptidoglycan by the pattern-recognition receptor PGRP-LE was crucial for the induction of autophagy and that autophagy prevented the intracellular growth of Listeria monocytogenes and promoted host survival after this infection. Autophagy induction occurred independently of the Toll and IMD innate signaling pathways. Our findings define a pathway leading from the intracellular pattern-recognition receptors to the induction of autophagy to host defense.


Assuntos
Autofagia , Drosophila/imunologia , Drosophila/metabolismo , Imunidade Inata/imunologia , Listeria/imunologia , Peptidoglicano/metabolismo , Animais , Ácido Diaminopimélico , Drosophila/genética , Drosophila/crescimento & desenvolvimento , Listeria/citologia , Peptidoglicano/imunologia , Receptores Toll-Like/imunologia
2.
Proc Natl Acad Sci U S A ; 112(18): 5809-14, 2015 May 05.
Artigo em Inglês | MEDLINE | ID: mdl-25901322

RESUMO

The innate immune system is the first line of defense encountered by invading pathogens. Delayed and/or inadequate innate immune responses can result in failure to combat pathogens, whereas excessive and/or inappropriate responses cause runaway inflammation. Therefore, immune responses are tightly regulated from initiation to resolution and are repressed during the steady state. It is well known that glycans presented on pathogens play important roles in pathogen recognition and the interactions between host molecules and microbes; however, the function of glycans of host organisms in innate immune responses is less well known. Here, we show that innate immune quiescence and strength of the immune response are controlled by host glycosylation involving a novel UDP-galactose transporter called Senju. In senju mutants, reduced expression of galactose-containing glycans resulted in hyperactivation of the Toll signaling pathway in the absence of immune challenges. Genetic epistasis and biochemical analyses revealed that Senju regulates the Toll signaling pathway at a step that converts Toll ligand Spatzle to its active form. Interestingly, Toll activation in immune-challenged wild type (WT) flies reduced the expression of galactose-containing glycans. Suppression of the degalactosylation by senju overexpression resulted in reduced induction of Toll-dependent expression of an antimicrobial peptide, Drosomycin, and increased susceptibility to infection with Gram-positive bacteria. These data suggest that Senju-mediated galactosylation suppresses undesirable Toll signaling activation during the steady state; however, Toll activation in response to infection leads to degalactosylation, which raises the immune response to an adequate level and contributes to the prompt elimination of pathogens.


Assuntos
Proteínas de Drosophila/fisiologia , Drosophila melanogaster/imunologia , Glicosilação , Imunidade Inata , Proteínas de Transporte de Monossacarídeos/metabolismo , Proteínas de Transporte de Monossacarídeos/fisiologia , Animais , Animais Geneticamente Modificados , Proteínas de Drosophila/genética , Proteínas de Drosophila/metabolismo , Epistasia Genética , Galactose/química , Sistema Imunitário , Lectinas/química , Proteínas de Transporte de Monossacarídeos/genética , Mutação , Polissacarídeos/química , Recombinação Genética , Receptores Toll-Like/metabolismo
3.
Genes Cells ; 20(6): 521-42, 2015 Jun.
Artigo em Inglês | MEDLINE | ID: mdl-25940448

RESUMO

Glycan structures are synthesized by a series of reactions conducted by glycosylation-related (GR) proteins such as glycosyltransferases, glycan-modifying enzymes, and nucleotide-sugar transporters. For example, the common core region of glycosaminoglycans (GAGs) is sequentially synthesized by peptide-O-xylosyltransferase, ß1,4-galactosyltransferase I, ß1,3-galactosyltransferase II, and ß1,3-glucuronyltransferase. This raises the possibility that functional impairment of GR proteins involved in synthesis of the same glycan might result in the same phenotypic abnormality. To examine this possibility, comprehensive silencing of genes encoding GR and proteoglycan core proteins was conducted in Drosophila. Drosophila GR candidate genes (125) were classified into five functional groups for synthesis of GAGs, N-linked, O-linked, Notch-related, and unknown glycans. Spatiotemporally regulated silencing caused a range of malformed phenotypes that fell into three types: extra veins, thick veins, and depigmentation. The clustered phenotypes reflected the biosynthetic pathways of GAGs, Fringe-dependent glycan on Notch, and glycans placed at or near nonreducing ends (herein termed terminal domains of glycans). Based on the phenotypic clustering, CG33145 was predicted to be involved in formation of terminal domains. Our further analysis showed that CG33145 exhibited galactosyltransferase activity in synthesis of terminal N-linked glycans. Phenotypic clustering, therefore, has potential for the functional prediction of novel GR genes.


Assuntos
Inativação Gênica , Família Multigênica , Fenótipo , Interferência de RNA , Animais , Drosophila , Glicosilação , Glicosiltransferases/metabolismo , Dados de Sequência Molecular , Polissacarídeos/genética
4.
BMC Biol ; 13: 81, 2015 Oct 01.
Artigo em Inglês | MEDLINE | ID: mdl-26437768

RESUMO

BACKGROUND: The diversification of immune systems during evolution involves the expansion of particular gene families in given phyla. A better understanding of the metazoan immune system requires an analysis of the logic underlying such immune gene amplification. This analysis is now within reach due to the ease with which we can generate multiple mutations in an organism. In this paper, we analyze the contribution of the three Drosophila prophenoloxidases (PPOs) to host defense by generating single, double and triple mutants. PPOs are enzymes that catalyze the production of melanin at the site of infection and around parasites. They are the rate-limiting enzymes that contribute to the melanization reaction, a major immune mechanism of arthropods. The number of PPO-encoding genes is variable among insects, ranging from one in the bee to ten in the mosquito. RESULTS: By analyzing mutations alone and in combination, we ascribe a specific function to each of the three PPOs of Drosophila. Our study confirms that two PPOs produced by crystal cells, PPO1 and PPO2, contribute to the bulk of melanization in the hemolymph, upon septic or clean injury. In contrast, PPO3, a PPO restricted to the D. melanogaster group, is expressed in lamellocytes and contributes to melanization during the encapsulation process. Interestingly, another overlapping set of PPOs, PPO2 and PPO3, achieve melanization of the capsule upon parasitoid wasp infection. CONCLUSIONS: The use of single or combined mutations allowed us to show that each PPO mutant has a specific phenotype, and that knocking out two of three genes is required to abolish fully a particular function. Thus, Drosophila PPOs have partially overlapping functions to optimize melanization in at least two conditions: following injury or during encapsulation. Since PPO3 is restricted to the D. melanogaster group, this suggests that production of PPO by lamellocytes emerged as a recent defense mechanism against parasitoid wasps. We conclude that differences in spatial localization, immediate or late availability, and mode of activation underlie the functional diversification of the three Drosophila PPOs, with each of them having non-redundant but overlapping functions.


Assuntos
Catecol Oxidase/genética , Proteínas de Drosophila/genética , Drosophila melanogaster/enzimologia , Drosophila melanogaster/imunologia , Precursores Enzimáticos/genética , Imunidade Inata , Animais , Catecol Oxidase/metabolismo , Proteínas de Drosophila/metabolismo , Drosophila melanogaster/genética , Drosophila melanogaster/parasitologia , Precursores Enzimáticos/metabolismo , Feminino , Imunidade Inata/genética , Vespas/fisiologia
5.
PLoS Genet ; 6(12): e1001254, 2010 Dec 23.
Artigo em Inglês | MEDLINE | ID: mdl-21203496

RESUMO

Glycosylation plays crucial regulatory roles in various biological processes such as development, immunity, and neural functions. For example, α1,3-fucosylation, the addition of a fucose moiety abundant in Drosophila neural cells, is essential for neural development, function, and behavior. However, it remains largely unknown how neural-specific α1,3-fucosylation is regulated. In the present study, we searched for genes involved in the glycosylation of a neural-specific protein using a Drosophila RNAi library. We obtained 109 genes affecting glycosylation that clustered into nine functional groups. Among them, members of the RNA regulation group were enriched by a secondary screen that identified genes specifically regulating α1,3-fucosylation. Further analyses revealed that an RNA-binding protein, second mitotic wave missing (Swm), upregulates expression of the neural-specific glycosyltransferase FucTA and facilitates its mRNA export from the nucleus. This first large-scale genetic screen for glycosylation-related genes has revealed novel regulation of fucTA mRNA in neural cells.


Assuntos
Proteínas de Drosophila/genética , Proteínas de Drosophila/metabolismo , Drosophila melanogaster/metabolismo , Genômica , Animais , Drosophila melanogaster/genética , Glicosilação , Sistema Nervoso/metabolismo , Especificidade de Órgãos
6.
Cell Struct Funct ; 37(1): 55-63, 2012.
Artigo em Inglês | MEDLINE | ID: mdl-22251795

RESUMO

The Golgi apparatus is an intracellular organelle playing central roles in post-translational modification and in the secretion of membrane and secretory proteins. These proteins are synthesized in the endoplasmic reticulum (ER) and transported to the cis-, medial-and trans-cisternae of the Golgi. While trafficking through the Golgi, proteins are sequentially modified with glycan moieties by different glycosyltransferases. Therefore, it is important to analyze the glycosylation function of the Golgi at the level of cisternae. Markers widely used for cis-, medial- and trans-cisternae/trans Golgi network (TGN) in Drosophila are GM130, 120 kDa and Syntaxin16 (Syx16); however the anti-120 kDa antibody is no longer available. In the present study, Drosophila Golgi complex-localized glycoprotein-1 (dGLG1) was identified as an antigen recognized by the anti-120 kDa antibody. A monoclonal anti-dGLG1 antibody suitable for immunohistochemistry was raised in rat. Using these markers, the localization of glycosyltransferases and nucleotide-sugar transporters (NSTs) was studied at the cisternal level. Results showed that glycosyltransferases and NSTs involved in the same sugar modification are localized to the same cisternae. Furthermore, valuable functional information was obtained on the localization of novel NSTs with as yet incompletely characterized biochemical properties.


Assuntos
Proteínas de Drosophila/metabolismo , Drosophila melanogaster/metabolismo , Complexo de Golgi/metabolismo , Receptores de Fatores de Crescimento de Fibroblastos/biossíntese , Sialoglicoproteínas/biossíntese , Animais , Anticorpos Monoclonais , Transporte Biológico , Biomarcadores , Células Cultivadas , Drosophila melanogaster/citologia , Glicosilação , Glicosiltransferases/metabolismo , Complexo de Golgi/ultraestrutura , Imuno-Histoquímica , Processamento de Proteína Pós-Traducional , Ratos , Receptores de Fatores de Crescimento de Fibroblastos/imunologia , Sialoglicoproteínas/imunologia
7.
J Neurosci ; 30(32): 10703-19, 2010 Aug 11.
Artigo em Inglês | MEDLINE | ID: mdl-20702701

RESUMO

Recent studies have demonstrated protective roles for autophagy in various neurodegenerative disorders, including the polyglutamine diseases; however, the role of autophagy in retinal degeneration has remained unclear. Accumulation of activated rhodopsin in some Drosophila mutants leads to retinal degeneration, and although it is known that activated rhodopsin is degraded in endosomal pathways in normal photoreceptor cells, the contribution of autophagy to rhodopsin regulation has remained elusive. This study reveals that activated rhodopsin is degraded by autophagy in collaboration with endosomal pathways to prevent retinal degeneration. Light-dependent retinal degeneration in the Drosophila visual system is caused by the knockdown or mutation of autophagy-essential components, such as autophagy-related protein 7 and 8 (atg-7/atg-8), or genes essential for PE (phosphatidylethanolamine) biogenesis and autophagosome formation, including Phosphatidylserine decarboxylase (Psd) and CDP-ethanolamine:diacylglycerol ethanolaminephosphotransferase (Ept). The knockdown of atg-7/8 or Psd/Ept produced an increase in the amount of rhodopsin localized to Rab7-positive late endosomes. This rhodopsin accumulation, followed by retinal degeneration, was suppressed by overexpression of Rab7, which accelerated the endosomal degradation pathway. These results indicate a degree of cross talk between the autophagic and endosomal/lysosomal pathways. Importantly, a reduction in rhodopsin levels rescued Psd knockdown-induced retinal degeneration. Additionally, the Psd knockdown-induced retinal degeneration phenotype was enhanced by Ppt1 inactivation, which causes infantile neuronal ceroid lipofuscinosis, implying that autophagy plays a significant role in its pathogenesis. Collectively, the current data reveal that autophagy suppresses light-dependent retinal degeneration in collaboration with the endosomal degradation pathway and that rhodopsin is a key substrate for autophagic degradation in this context.


Assuntos
Autofagia/fisiologia , Células Fotorreceptoras de Invertebrados/metabolismo , Degeneração Retiniana/prevenção & controle , Rodopsina/metabolismo , Animais , Animais Geneticamente Modificados , Modelos Animais de Doenças , Drosophila , Proteínas de Drosophila/genética , Proteínas de Drosophila/metabolismo , Endossomos/metabolismo , Endossomos/ultraestrutura , Regulação da Expressão Gênica/genética , Regulação da Expressão Gênica/fisiologia , Proteínas de Fluorescência Verde/genética , Marcação In Situ das Extremidades Cortadas/métodos , Larva , Luz/efeitos adversos , Lisossomos/metabolismo , Lisossomos/ultraestrutura , Proteínas de Membrana/genética , Proteínas de Membrana/metabolismo , Microscopia Eletrônica de Transmissão/métodos , Microscopia Imunoeletrônica/métodos , Mutação/genética , Proteínas do Tecido Nervoso/genética , Proteínas do Tecido Nervoso/metabolismo , Células Fotorreceptoras de Invertebrados/ultraestrutura , Interferência de RNA/fisiologia , Degeneração Retiniana/etiologia , Degeneração Retiniana/genética , Rodopsina/genética , Estatísticas não Paramétricas , Tioléster Hidrolases , Fatores de Tempo , Proteínas rab de Ligação ao GTP/metabolismo , proteínas de unión al GTP Rab7
8.
J Cell Biol ; 174(6): 773-8, 2006 Sep 11.
Artigo em Inglês | MEDLINE | ID: mdl-16966419

RESUMO

Specific sulfation sequence of heparan sulfate (HS) contributes to the selective interaction between HS and various proteins in vitro. To clarify the in vivo importance of HS fine structures, we characterized the functions of the Drosophila HS 2-O and 6-O sulfotransferase (Hs2st and Hs6st) genes in FGF-mediated tracheal formation. We found that mutations in Hs2st or Hs6st had unexpectedly little effect on tracheal morphogenesis. Structural analysis of mutant HS revealed not only a loss of corresponding sulfation, but also a compensatory increase of sulfation at other positions, which maintains the level of HS total charge. The restricted phenotypes of Hsst mutants are ascribed to this compensation because FGF signaling is strongly disrupted by Hs2st; Hs6st double mutation, or by overexpression of 6-O sulfatase, an extracellular enzyme which removes 6-O sulfate groups without increasing 2-O sulfation. These findings suggest that the overall sulfation level is more important than strictly defined HS fine structures for FGF signaling in some developmental contexts.


Assuntos
Drosophila melanogaster/embriologia , Fatores de Crescimento de Fibroblastos/metabolismo , Heparitina Sulfato/metabolismo , Sulfotransferases/metabolismo , Traqueia/embriologia , Animais , Drosophila melanogaster/genética , Drosophila melanogaster/metabolismo , Feminino , Regulação da Expressão Gênica no Desenvolvimento/fisiologia , Regulação Enzimológica da Expressão Gênica/fisiologia , Heparitina Sulfato/química , Masculino , Estrutura Molecular , Mutação/genética , Organogênese/genética , Transdução de Sinais/fisiologia , Sulfotransferases/genética , Ésteres do Ácido Sulfúrico/metabolismo , Traqueia/citologia , Traqueia/metabolismo
9.
Neuron ; 50(6): 855-67, 2006 Jun 15.
Artigo em Inglês | MEDLINE | ID: mdl-16772168

RESUMO

Axon pruning is a common phenomenon in neural circuit development. Previous studies demonstrate that the engulfing action of glial cells is essential in this process. The underlying molecular mechanisms, however, remain unknown. We show that draper (drpr) and ced-6, which are essential for the clearance of apoptotic cells in C. elegans, function in the glial engulfment of larval axons during Drosophila metamorphosis. The drpr mutation and glia-specific knockdown of drpr and ced-6 by RNA interference suppress glial engulfment, resulting in the inhibition of axon pruning. drpr and ced-6 interact genetically in the glial action. Disruption of the microtubule cytoskeleton in the axons to be pruned occurs via ecdysone signaling, independent of glial engulfment. These findings suggest that glial cells engulf degenerating axons through drpr and ced-6. We propose that apoptotic cells and degenerating axons of living neurons are removed by a similar molecular mechanism.


Assuntos
Apoptose/fisiologia , Axônios/fisiologia , Proteínas de Caenorhabditis elegans/fisiologia , Proteínas de Drosophila/fisiologia , Proteínas de Membrana/fisiologia , Metamorfose Biológica/fisiologia , Fosfoproteínas/fisiologia , Animais , Animais Geneticamente Modificados , Proteínas Reguladoras de Apoptose , Drosophila , Regulação da Expressão Gênica no Desenvolvimento/fisiologia , Rede Nervosa , Ratos
10.
Neuron ; 52(6): 997-1009, 2006 Dec 21.
Artigo em Inglês | MEDLINE | ID: mdl-17178403

RESUMO

Local control of mRNA translation modulates neuronal development, synaptic plasticity, and memory formation. A poorly understood aspect of this control is the role and composition of ribonucleoprotein (RNP) particles that mediate transport and translation of neuronal RNAs. Here, we show that staufen- and FMRP-containing RNPs in Drosophila neurons contain proteins also present in somatic "P bodies," including the RNA-degradative enzymes Dcp1p and Xrn1p/Pacman and crucial components of miRNA (argonaute), NMD (Upf1p), and general translational repression (Dhh1p/Me31B) pathways. Drosophila Me31B is shown to participate (1) with an FMRP-associated, P body protein (Scd6p/trailer hitch) in FMRP-driven, argonaute-dependent translational repression in developing eye imaginal discs; (2) in dendritic elaboration of larval sensory neurons; and (3) in bantam miRNA-mediated translational repression in wing imaginal discs. These results argue for a conserved mechanism of translational control critical to neuronal function and open up new experimental avenues for understanding the regulation of mRNA function within neurons.


Assuntos
Proteínas de Drosophila/fisiologia , Proteína do X Frágil da Deficiência Intelectual/metabolismo , Neurônios/metabolismo , Proteínas de Ligação a RNA/metabolismo , Ribonucleoproteínas/fisiologia , Animais , Animais Geneticamente Modificados , Northern Blotting , Western Blotting/métodos , Caspases/metabolismo , Células Cultivadas , Sistema Nervoso Central/citologia , Dendritos/metabolismo , Dendritos/fisiologia , Drosophila , Proteínas de Drosophila/metabolismo , Exorribonucleases/metabolismo , Olho/metabolismo , Olho/ultraestrutura , Proteínas de Fluorescência Verde/metabolismo , Imuno-Histoquímica/métodos , Larva , MicroRNAs/metabolismo , Microscopia Eletrônica de Varredura/métodos , Neurônios/citologia , Biossíntese de Proteínas/fisiologia , Transporte Proteico/fisiologia , Complexo de Inativação Induzido por RNA/metabolismo
11.
PLoS Biol ; 5(11): e315, 2007 Nov.
Artigo em Inglês | MEDLINE | ID: mdl-18044989

RESUMO

Animal circadian clocks are based on multiple oscillators whose interactions allow the daily control of complex behaviors. The Drosophila brain contains a circadian clock that controls rest-activity rhythms and relies upon different groups of PERIOD (PER)-expressing neurons. Two distinct oscillators have been functionally characterized under light-dark cycles. Lateral neurons (LNs) that express the pigment-dispersing factor (PDF) drive morning activity, whereas PDF-negative LNs are required for the evening activity. In constant darkness, several lines of evidence indicate that the LN morning oscillator (LN-MO) drives the activity rhythms, whereas the LN evening oscillator (LN-EO) does not. Since mutants devoid of functional CRYPTOCHROME (CRY), as opposed to wild-type flies, are rhythmic in constant light, we analyzed transgenic flies expressing PER or CRY in the LN-MO or LN-EO. We show that, under constant light conditions and reduced CRY function, the LN evening oscillator drives robust activity rhythms, whereas the LN morning oscillator does not. Remarkably, light acts by inhibiting the LN-MO behavioral output and activating the LN-EO behavioral output. Finally, we show that PDF signaling is not required for robust activity rhythms in constant light as opposed to its requirement in constant darkness, further supporting the minor contribution of the morning cells to the behavior in the presence of light. We therefore propose that day-night cycles alternatively activate behavioral outputs of the Drosophila evening and morning lateral neurons.


Assuntos
Relógios Biológicos/fisiologia , Encéfalo/citologia , Ritmo Circadiano/fisiologia , Drosophila/fisiologia , Neurônios/citologia , Fotoperíodo , Animais , Comportamento Animal , Encéfalo/metabolismo , Proteínas de Drosophila/genética , Proteínas de Drosophila/metabolismo , Expressão Gênica , Genótipo , Luz , Atividade Motora/fisiologia , Neurônios/metabolismo , Neuropeptídeos/genética , Neuropeptídeos/metabolismo , Proteínas Nucleares/genética , Proteínas Nucleares/metabolismo , Organismos Geneticamente Modificados , Proteínas Circadianas Period , Transdução de Sinais
12.
Mol Biol Cell ; 18(4): 1519-29, 2007 Apr.
Artigo em Inglês | MEDLINE | ID: mdl-17314398

RESUMO

ATF-2 is a member of the ATF/CREB family of transcription factors that is activated by stress-activated protein kinases such as p38. To analyze the physiological role of Drosophila ATF-2 (dATF-2), we generated dATF-2 knockdown flies using RNA interference. Reduced dATF-2 in the fat body, the fly equivalent of the mammalian liver and adipose tissue, decreased survival under starvation conditions. This was due to smaller triglyceride reserves of dATF-2 knockdown flies than control flies. Among multiple genes that control triglyceride levels, expression of the Drosophila PEPCK (dPEPCK) gene was strikingly reduced in dATF-2 knockdown flies. PEPCK is a key enzyme for both gluconeogenesis and glyceroneogenesis, which is a pathway required for triglyceride synthesis via glycerol-3-phosphate. Although the blood sugar level in dATF-2 knockdown flies was almost same as that in control flies, the activity of glyceroneogenesis was reduced in the fat bodies of dATF-2 knockdown flies. Thus, reduced glyceroneogenesis may at least partly contribute to decreased triglyceride stores in the dATF-2 knockdown flies. Furthermore we showed that dATF-2 positively regulated dPEPCK gene transcription via several CRE half-sites in the PEPCK promoter. Thus, dATF-2 is critical for regulation of fat metabolism.


Assuntos
Tecido Adiposo/metabolismo , Fatores de Transcrição de Zíper de Leucina Básica/metabolismo , Proteínas de Drosophila/metabolismo , Drosophila/metabolismo , Fator 2 Ativador da Transcrição , Tecido Adiposo/citologia , Animais , Animais Geneticamente Modificados , Fatores de Transcrição de Zíper de Leucina Básica/genética , Tamanho Celular , Drosophila/genética , Proteínas de Drosophila/genética , Metabolismo Energético , Regulação da Expressão Gênica , Masculino , Camundongos , Fosfoenolpiruvato Carboxiquinase (ATP)/genética , Fosfoenolpiruvato Carboxiquinase (ATP)/metabolismo , Interferência de RNA , Inanição , Taxa de Sobrevida , Transcrição Gênica , Triglicerídeos/metabolismo
13.
Curr Biol ; 16(16): 1678-83, 2006 Aug 22.
Artigo em Inglês | MEDLINE | ID: mdl-16920632

RESUMO

Neurons are highly polarized cells with distinct subcellular compartments, including dendritic arbors and an axon. The proper function of the nervous system relies not only on correct targeting of axons, but also on development of neuronal-class-specific geometry of dendritic arbors [1-4]. To study the intercellular control of the shaping of dendritic trees in vivo, we searched for cell-surface proteins expressed by Drosophila dendritic arborization (da) neurons [5-7]. One of them was Neuroglian (Nrg), a member of the Ig superfamily ; Nrg and vertebrate L1-family molecules have been implicated in various aspects of neuronal wiring, such as axon guidance, axonal myelination, and synapse formation [9-12]. A subset of the da neurons in nrg mutant embryos exhibited deformed dendritic arbors and abnormal axonal sprouting. Our functional analysis in a cell-type-selective manner strongly suggested that those da neurons employed Nrg to interact with the peripheral glia for suppressing axonal sprouting and for forming second-order dendritic branches. At least for the former role, Nrg functioned in concert with the intracellular adaptor protein Ankyrin (Ank) [13]. Thus, the neuron-glia interaction that is mediated by Nrg, together with Ank under some situations, contributes to axonal and dendritic morphogenesis.


Assuntos
Anquirinas/metabolismo , Axônios/fisiologia , Moléculas de Adesão Celular Neuronais/metabolismo , Dendritos/fisiologia , Proteínas de Drosophila/metabolismo , Drosophila/embriologia , Sistema Nervoso/embriologia , Neuroglia/metabolismo , Animais , Anquirinas/genética , Moléculas de Adesão Celular Neuronais/genética , Proteínas de Drosophila/genética , Componentes do Gene , Sistema Nervoso/citologia , Sistema Nervoso/metabolismo
14.
Curr Biol ; 16(15): 1531-7, 2006 Aug 08.
Artigo em Inglês | MEDLINE | ID: mdl-16887350

RESUMO

Differentiated cells assume complex shapes through polarized cell migration and growth. These processes require the restricted organization of the actin cytoskeleton at limited subcellular regions. IKK epsilon is a member of the IkappaB kinase family, and its developmental role has not been clear. Drosophila IKK epsilon was localized to the ruffling membrane of cultured cells and was required for F actin turnover at the cell margin. In IKK epsilon mutants, tracheal terminal cells, bristles, and arista laterals, which require accurate F actin assembly for their polarized elongation, all exhibited aberrantly branched morphology. These phenotypes were sensitive to a change in the dosage of Drosophila inhibitor of apoptosis protein 1 (DIAP1) and the caspase DRONC without apparent change in cell viability. In contrast to this, hyperactivation of IKK epsilon destabilized F actin-based structures. Expression of a dominant-negative form of IKK epsilon increased the amount of DIAP1. The results suggest that at the physiological level, IKK epsilon acts as a negative regulator of F actin assembly and maintains the fidelity of polarized elongation during cell morphogenesis. This IKK epsilon function involves the negative regulation of the nonapoptotic activity of DIAP1.


Assuntos
Actinas/metabolismo , Apoptose/fisiologia , Diferenciação Celular/fisiologia , Proteínas de Drosophila/metabolismo , Drosophila/fisiologia , Quinase I-kappa B/metabolismo , Proteínas Inibidoras de Apoptose/metabolismo , Morfogênese/fisiologia , Animais , Western Blotting , Testes Genéticos , Histocitoquímica , Microscopia de Fluorescência , Traqueia/citologia , Traqueia/crescimento & desenvolvimento
15.
Curr Biol ; 16(8): 808-13, 2006 Apr 18.
Artigo em Inglês | MEDLINE | ID: mdl-16631589

RESUMO

Unlike mammalian Toll-like Receptors, the Drosophila Toll receptor does not interact directly with microbial determinants but is rather activated upon binding a cleaved form of the cytokine-like molecule Spatzle (Spz). During the immune response, Spz is thought to be processed by secreted serine proteases (SPs) present in the hemolymph that are activated by the recognition of gram-positive bacteria or fungi . In the present study, we have used an in vivo RNAi strategy to inactivate 75 distinct Drosophila SP genes. We then screened this collection for SPs regulating the activation of the Toll pathway by gram-positive bacteria. Here, we report the identification of five novel SPs that function in an extracellular pathway linking the recognition proteins GNBP1 and PGRP-SA to Spz. Interestingly, four of these genes are also required for Toll activation by fungi, while one is specifically associated with signaling in response to gram-positive bacterial infections. These results demonstrate the existence of a common cascade of SPs upstream of Spz, integrating signals sent by various secreted recognition molecules via more specialized SPs.


Assuntos
Proteínas de Drosophila/metabolismo , Drosophila/imunologia , Serina Endopeptidases/fisiologia , Receptores Toll-Like/metabolismo , Animais , Proteínas de Transporte/metabolismo , Enterococcus faecalis , Infecções por Bactérias Gram-Positivas/imunologia , Micrococcus luteus , Interferência de RNA , Serina Endopeptidases/genética
16.
J Cell Biol ; 166(7): 1069-79, 2004 Sep 27.
Artigo em Inglês | MEDLINE | ID: mdl-15452147

RESUMO

Heparan sulfate (HS) regulates the activity of various ligands and is involved in molecular recognition events on the cell surface and in the extracellular matrix. Specific binding of HS to different ligand proteins depends on the sulfation pattern of HS. For example, the interaction between antithrombin and a particular 3-O sulfated HS motif is thought to modulate blood coagulation. However, a recent study of mice defective for this modification suggested that 3-O sulfation plays other biological roles. Here, we show that Drosophila melanogaster HS 3-O sulfotransferase-b (Hs3st-B), which catalyzes HS 3-O sulfation, is a novel component of the Notch pathway. Reduction of Hs3st-B function by transgenic RNA interference compromised Notch signaling, producing neurogenic phenotypes. We also show that levels of Notch protein on the cell surface were markedly decreased by loss of Hs3st-B. These findings suggest that Hs3st-B is involved in Notch signaling by affecting stability or intracellular trafficking of Notch protein.


Assuntos
Proteínas de Drosophila/metabolismo , Drosophila melanogaster/metabolismo , Heparitina Sulfato/metabolismo , Proteínas de Membrana/metabolismo , Transdução de Sinais/fisiologia , Sulfotransferases/metabolismo , Sequência de Aminoácidos , Animais , Animais Geneticamente Modificados , Sequência de Bases/genética , Membrana Celular/genética , Membrana Celular/metabolismo , DNA Complementar/análise , DNA Complementar/genética , Regulação para Baixo/genética , Proteínas de Drosophila/genética , Proteínas de Drosophila/isolamento & purificação , Drosophila melanogaster/embriologia , Drosophila melanogaster/genética , Matriz Extracelular/genética , Matriz Extracelular/metabolismo , Regulação da Expressão Gênica no Desenvolvimento/genética , Membranas Intracelulares/metabolismo , Proteínas de Membrana/genética , Dados de Sequência Molecular , Fenótipo , Ligação Proteica/genética , Transporte Proteico/genética , Interferência de RNA , Receptores Notch , Transdução de Sinais/genética , Sulfotransferases/genética , Sulfotransferases/isolamento & purificação
17.
Glycobiology ; 18(12): 1094-104, 2008 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-18824562

RESUMO

T antigen (Galbeta1-3GalNAcalpha1-Ser/Thr), the well-known tumor-associated antigen, is a core 1 mucin-type O-glycan structure that is synthesized by core 1 beta1,3-galactosyltransferase (C1beta3GalT), which transfers Gal from UDP-Gal to Tn antigen (GalNAcalpha1-Ser/Thr). Three putative C1beta3GalTs have been identified in Drosophila. However, although all three are expressed in embryos, their roles during embryogenesis have not yet been clarified. In this study, we used P-element inserted mutants to show that CG9520, one of the three putative C1beta3GalTs, synthesizes T antigen expressed on the central nervous system (CNS) during embryogenesis. We also found that T antigen was expressed on a subset of the embryonic hemocytes. CG9520 mutant embryos showed the loss of T antigens on the CNS and on a subset of hemocytes. Then, the loss of T antigens was rescued by precise excision of the P-element inserted into the CG9520 gene. Our data demonstrate that T antigens expressed on the CNS and on a subset of hemocytes are synthesized by CG9520 in the Drosophila embryo. In addition, we found that the number of circulating hemocytes was reduced in third instar larvae of CG9520 mutant. We, therefore, named the CG9520 gene Drosophila core 1 beta1,3-galactosyltransferase 1 because it is responsible for the synthesis and function of T antigen in vivo.


Assuntos
Antígenos Glicosídicos Associados a Tumores/biossíntese , Proteínas de Drosophila/genética , Drosophila/embriologia , Drosophila/enzimologia , Galactosiltransferases/genética , Hemócitos/metabolismo , Animais , Antígenos Glicosídicos Associados a Tumores/imunologia , Sistema Nervoso Central/metabolismo , Drosophila/genética , Embrião não Mamífero/enzimologia , Histocitoquímica , Mutação , Filogenia
18.
Gene ; 425(1-2): 64-8, 2008 Dec 01.
Artigo em Inglês | MEDLINE | ID: mdl-18775483

RESUMO

The Tol2 element is a transposon found from a genome of a vertebrate, a small teleost medaka fish. Tol2 encodes a gene for a transposase which is active in vertebrate animals so far tested; for instance, in fish, frog, chicken and mammals, and transgenesis methods using Tol2 have been developed in these model vertebrates. However, it has not been known whether Tol2 can transpose in animals other than vertebrates. Here we report transposition of Tol2 in an invertebrate Drosophila melanogaster. First, we injected a transposon donor plasmid containing a Tol2 construct and mRNA encoding the Tol2 transposase into Drosophila eggs, and found that the Tol2 construct could be excised from the plasmid. Second, we crossed the injected flies, raised the offspring, and found that the Tol2 construct was integrated into the genome of germ cells and transmitted to the next generation. Finally, we constructed a Tol2 construct containing the white gene and injected the transposon donor plasmid and the transposase mRNA into fertilized eggs from the white mutant. We analyzed their offspring, and found that G1 flies with wild type red eyes could be obtained from 35% of the injected fly. We cloned and sequenced 34 integration loci from these lines and showed that these insertions were indeed created through transposition and distributed throughout the genome. Our present study demonstrates that the medaka fish Tol2 transposable element does not require vertebrate-specific host factors for its transposition, and also provides a possibility that Tol2 may be used as a new genetic tool for transgenesis and genome analysis in Drosophila.


Assuntos
Elementos de DNA Transponíveis , Drosophila melanogaster/genética , Técnicas de Transferência de Genes , Oryzias/genética , Animais , Clonagem Molecular , Transfecção
19.
Genetics ; 177(2): 1233-7, 2007 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-17660557

RESUMO

We identified the causal genetic variation for the difference in the thoracic trident pigmentation intensity between two wild-derived strains of Drosophila melanogaster. It was found to be the difference in expression level of ebony, which codes for an enzyme in the melanin-synthesis pathway and has pleiotropic effects on vision and behavior.


Assuntos
Proteínas de Ligação a DNA/genética , Proteínas de Drosophila/genética , Drosophila melanogaster/genética , Variação Genética , Pigmentação/genética , Tórax , Animais , Sequência de Bases , Genes de Insetos , Dados de Sequência Molecular
20.
Mol Cell Biol ; 25(8): 3140-50, 2005 Apr.
Artigo em Inglês | MEDLINE | ID: mdl-15798200

RESUMO

Growth, patterning, and apoptosis are mutually interactive during development. For example, cells that select an abnormal fate in a developing field are frequently removed by apoptosis. An important issue in this process that needs to be resolved is the mechanism used by cells to discern their correct fate from an abnormal fate. In order to examine this issue, we developed an animal model that expresses the dioxin receptor homolog Spineless (Ss) ectopically in the Drosophila wing. The presence of mosaic clones ectopically expressing ss results in a local transformation of organ identity, homeosis, from wing into a leg or antenna. The cells with misspecified fates subsequently activate c-Jun N-terminal kinase to undergo apoptosis in an autonomous or nonautonomous manner depending on their position within the wing, suggesting that a cell-cell interaction is, at least in some cases, involved in the detection of misspecified cells. Similar position dependence is commonly observed when various homeotic genes controlling the body segments are ectopically expressed. The autonomous and nonautonomous apoptosis caused by ss is regulated by a novel leucine-rich repeat family transmembrane protein, Fish-lips (Fili) that interacts with surrounding normal cells. These data support a mechanism in which the lack of some membrane proteins helps to recognize the presence of different cell types and direct these cells to an apoptotic fate in order to exclude them from the normal developing field.


Assuntos
Apoptose/fisiologia , Padronização Corporal/fisiologia , Proteínas de Drosophila/genética , Proteínas de Drosophila/metabolismo , Proteínas de Drosophila/fisiologia , Drosophila melanogaster/crescimento & desenvolvimento , Proteínas de Membrana/genética , Proteínas de Membrana/fisiologia , Receptores de Hidrocarboneto Arílico/metabolismo , Motivos de Aminoácidos , Sequência de Aminoácidos , Animais , Apoptose/genética , Padronização Corporal/genética , Drosophila melanogaster/genética , Drosophila melanogaster/fisiologia , Extremidades/crescimento & desenvolvimento , Expressão Gênica , Proteínas de Repetições Ricas em Leucina , Dados de Sequência Molecular , Mutação/genética , Estrutura Terciária de Proteína , Proteínas/genética , Proteínas/fisiologia , RNA Mensageiro/análise , RNA Mensageiro/metabolismo , Receptores de Hidrocarboneto Arílico/genética , Asas de Animais/crescimento & desenvolvimento
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