Functional central nervous system myelin repair in an adult mouse model of demyelination caused by proteolipid protein overexpression.

Two types of interventions to remyelinate the adult demyelinated central nervous system were investigated in heterozygous transgenic mice overexpressing the proteolipid protein gene. 1) A cocktail of trophic factors, "TS1," was directed toward the activation of the endogenous pool of neural progenitors to increase the number of myelinating oligodendrocytes (OL) in the brain. 2) A combinatorial approach in which OL progenitors were coinjected with TS1 into the corpus callosum of wild-type and He4e transgenic mice that displayed hindlimb paralysis. The levels of locomotor ability in these mice were evaluated after a single treatment. The data showed that a single administration of either one of the interventions had similar therapeutic effects, alleviating the symptoms of demyelination and leading to the recovery of hindlimb function. Histological and immunofluorescent examination of brain sections showed extensive remyelination that was sufficient to reverse hindlimb paralysis in transgenic mice. When the interventions were administered prior to hindlimb paralysis, He4e mice were able to walk up to 1 year of age without paralysis.

Effect of pentosanase and oxidases on the characteristics of doughs and the glutenin macropolymer (GMP).

Rheological characteristics of dough and glutenin macropolymer (GMP) extracted thereof were investigated. Three single enzymes, pentosanase (PP), glucoseoxidase (GLZ), and laccase (LAC), and their combinations were used. GLZ gave the least extensible and most resistant dough, and pentosanase/glucoseoxidase (PPGLZ) resulted in dough with improved extensibility. The enzymes improved gluten quality. The glutenin macropolymer (GMP) was characterized in terms of wet weight, protein content, pentosan association, and dynamic rheological properties. Enzymatic addition decreased the wet weight of GMP but increased the protein content. PP decreased the content of pentosans on the GMP, but single oxidases increased the content of pentosans associated with GMP. PP did not modify the elastic modulus (G') of the GMP, whereas GLZ increased G' by increasing the polymerization of proteins and LAC diminished G'. The combination PPGLZ produced a synergic increase of G'.

Isolation and identification of anaerobic bacteria from ovine foot rot in Spain.

A microbiological study was made of 125 Merino sheep showing clinical signs of foot rot. A total of 435 strictly anaerobic strains were isolated, belonging to the following genera: Bacteroides, Peptostreptococcus, Tissierella, Fusobacterium, Megasphaera, Eubacterium, Acidaminococcus, Clostridium, Peptococcus and Propionibacterium. Of the 35 species obtained, the following were found in more than 10 per cent of animals sampled: Bacteroides nodosus, B putredinis, B buccae, B ruminicola subspecies brevis, Tissierella praeacuta, Peptostreptococcus anaerobius and Megasphaera elsdenii. Six culture media were used for isolation. Agar brucella and agar brucella enriched with G-N anaerobe supplement proved to be the most efficient for isolating anaerobic bacteria.

Perceived health needs of inner-city street prostitutes: a preliminary study.

OBJECTIVE: To survey inner-city prostitutes' perceived health needs. METHODS: One hundred forty street prostitutes in Washington DC, were surveyed to determine their perceived health needs, in addition to levels of post traumatic stress disorder (PTSD). RESULTS: The final sample comprised 100 individuals, representing 3 subgroups of prostitutes, female, male, and transgender male. Major health needs included protection from physical and sexual assault, social support, counseling, addictions treatment, job training, and medical care. Over 42% of the population was identified as meeting established criteria for PTSD. CONCLUSION: Effective program development for inner-city prostitutes needs to acknowledge the presence of distinct subpopulations and the pervasive influence of PTSD on health status.

Drug polyconsumption is associated with increased synchronization of brain electrical-activity at rest and in a counting task.

Drug abusers typically consume not just one but several types of drugs, starting from alcohol and marijuana consumption, and then dramatically lapsing into addiction to harder drugs, such as cocaine, heroin, or amphetamine. The brain of drug abusers presents various structural and neurophysiological abnormalities, some of which may predate drug consumption onset. However, how these changes translate into modifications in functional brain connectivity is still poorly understood. To characterize functional connectivity patterns, we recorded Electroencephalogram (EEG) activity from 21 detoxified drug abusers and 20 age-matched control subjects performing a simple counting task and at rest activity. To evaluate the cortical brain connectivity network we applied the Synchronization Likelihood algorithm. The results showed that drug abusers had higher synchronization levels at low frequencies, mainly in the θ band (4-8 Hz) between frontal and posterior cortical regions. During the counting task, patients showed increased synchronization in the ß (14-35 Hz), and γ (35-45 Hz) frequency bands, in fronto-posterior and interhemispheric temporal regions. Taken together 'slow-down' at rest and task-related 'over-exertion' could indicate that the brain of drug abusers is suffering from a premature form of ageing. Future studies will clarify whether this condition can be reversed following prolonged periods of abstinence.

[Early gastric cancer in the remnant stomach. Case report]. / Cancer gastrico precoz en la boca anastomotica. Presentacion de un caso.

Patients with partial gastrectomy are a High risk group to develop gastric cancer. Risk is higher in remnant estomach after partial gastrectomy by malignancy than benign disease. We present a case of a male patient, 64 years old, who was admitted in our Hospital with Hypovolaemia. We found during Endoscopic examination early gastric Cancer IIc + III type in the site of anastomosis. Partial gastrectomy was performed in this patient by Duodenal ulcer 11 years ago.

Comparative in vitro susceptibility of Bacteroides and Fusobacterium isolated from footrot in sheep to 28 antimicrobial agents.

The agar dilution method was used to determine the bacteriostatic activity of 28 antimicrobial agents against 141 strains to the genus Bacteroides and 29 strains from the genus Fusobacterium. All organisms were isolated from clinical cases of ovine footrot. The strains were isolated from 125 Merino sheep, over a period of 2 years, from January 1987 to December 1988. The three ureidopenicillins studied (azlocillin, mezlocillin and piperacillin) proved to be the most effective antimicrobial agents. Chloramphenicol, metronidazole and tinidazole effectively inhibited the growth of Bacteroides spp., while phosphomycin was active against Fusobacterium spp.

[Pancreatic pseudocyst. A review of 10 years]. / Pseudoquiste pancreático. 10 años de revisión.

In the hope of finding those patients whose pancreas pseudocyst resolution were spontaneous, we undertook a retrospective review of such cases admitted at the Hospital Central de San Cristobal y Hospital Patrocinio Peñuela Ruiz (from IVSS), with the diagnosis of acute pancreatitis. From 619 patients with pancreatitis, 40 developed a pseudocyst (5.78%). The most frequent ethiology was biliary disease (47.5%) and abdominal pain in 87.5%, the most common symptom. Abdominal ultrasonography was the best diagnostic aid. Spontaneous resolution occur in 24 cases (60%) in juntion with the normalization of seric and urinary amylases values, the size of the cyst in these patients was less than 5 cms. Sixteen patients needed surgery, in 8 of them the seric amylase value remained high and in 3 cases this value was normal but with a cyst size more than five cms. Internal drainage in 11, external in 4 and surgical resection in one. There was no deaths in this review.

In-vitro antimicrobial susceptibility of Bacteroides and Fusobacterium isolated from footrot in goats.

The agar dilution method was used to determine the antimicrobial activity of 17 antimicrobial agents against 132 strains belonging to the genus Bacteroides and 25 strains from the genus Fusobacterium, all isolated from 120 clinical cases of caprine footrot between October 1987 and November 1988. Josamycin, chloramphenicol and rifampin proved to be the most effective antibiotics in vitro. Significant resistance was found to the other antimicrobial agents studied.

Susceptibilities of Bacteroides and Fusobacterium spp. from foot rot in goats to 10 beta-lactam antibiotics.

The agar dilution method was used to determine the bacteriostatic activities of 10 beta-lactam antibiotics against 132 strains belonging to the genus Bacteroides and 25 strains belonging to the genus Fusobacterium, all isolated from clinical cases of caprine foot rot. The three ureidopenicillins studied proved to be the most effective antimicrobial agents.

Obligately anaerobic bacterial species isolated from foot-rot lesions in goats.

Lesions showing clinical signs of foot-rot from 120 goats were cultured on six selective media during October 1987 to November 1988. A total of 582 strictly anaerobic microorganisms belonging to 50 different species was isolated and identified. The anaerobes most frequently isolated belonged to the following genera: Bacteroides (80%), Peptostreptococcus (63.6%), Megasphaera (40%), Fusobacterium (29.2%), Clostridium (22.5%), Propionibacterium (12.5%), Eubacterium (11.7%) and Leptotrichia (10.8%). Percentages for Acidaminococcus, Peptococcus, Tissierella, Wolinella and Veillonella were below 10%. The following species were identified in 10% or more of cases: Peptostreptococcus anaerobius (61.7%), Bacteroides buccae (51.7%), Bacteroides nodosus (42.5%), Megasphaera elsdenii (40%), Bacteroides ruminicola subsp. brevis (22.5%), Fusobacterium necrophorum (19.2%), Leptotrichia buccalis (11.7%) and Clostridium perfringens (10%). Lower percentages were obtained for the remaining species. The efficiency and selectivity of the six culture media used for isolation are discussed.

[Splenic abscess, a diagnostic and therapeutic problem]. / El absceso esplénico, un problema diagnóstico y terapeútico.

Splenic abscess remains a rare entity, reserved for patients with predisposing factors or immunosuppressive conditions. In our 13 patients in which a splenic abscess was diagnosed pre- or intra-operatively, an average of 14 days past before the diagnosis was made. Main symptoms were fever, upper left abdominal pain, auscultatory left basal anomalies and splenomegaly. Culture from the spleen material included E. coli, Staphylococcus, Proteus, Salmonella and Streptococcus v. Although splenectomy has been considered the standard procedure for this pathology, we treated our last three patients by means of Computed Tomography Percutaneous Drainage (C.T.G.P.D.). There was no need for splenectomy after this procedure and there were no complications associated with the C. T. G. P. D.

Colangiografia fluoroscópica transoperatoria: una mejor alternativa en cirugía laparoscópica / Fluoroscopic transoperative cholangiograpy: a better alternative in laparoscopy surgery

La endoscolecistectomia es la cirugía que se realiza más frecuentemente. La colangiografía trasoperatoria se realiza en todo paciente con sospecha de coledocolitiasis. La colangiografía estática convencional es usada entre los cirujanos de vías biliares. Nuestro objetivo es dar a conocer las ventajas de la fluoroscopia tales como menor irradiación, reducción del tiempo operatorio, mejor relación costo beneficio, mayor fidelidad para el diagnóstico de espasmo del esfinter de Oddi así como la facilidad para descartar artefactos. Presentamos la experiencia en 13 pacientes sometidos a endocolecistectomía en el Centro Clínico San Cristobal a quienes se les realizó colangiografía fluoroscópica transoperatoria