4-Azidocinnoline-Cinnoline-4-amine Pair as a New Fluorogenic and Fluorochromic Environment-Sensitive Probe.

A new type of fluorogenic and fluorochromic probe based on the reduction of weakly fluorescent 4-azido-6-(4-cyanophenyl)cinnoline to the corresponding fluorescent cinnoline-4-amine was developed. We found that the fluorescence of 6-(4-cyanophenyl)cinnoline-4-amine is strongly affected by the nature of the solvent. The fluorogenic effect for the amine was detected in polar solvents with the strongest fluorescence increase in water. The environment-sensitive fluorogenic properties of cinnoline-4-amine in water were explained as a combination of two types of fluorescence mechanisms: aggregation-induced emission (AIE) and excited state intermolecular proton transfer (ESPT). The suitability of an azide-amine pair as a fluorogenic probe was tested using a HepG2 hepatic cancer cell line with detection by fluorescent microscopy, flow cytometry, and HPLC analysis of cells lysates. The results obtained confirm the possibility of the transformation of the azide to amine in cells and the potential applicability of the discovered fluorogenic and fluorochromic probe for different analytical and biological applications in aqueous medium.

Concerns and Challenges Related to Sputnik V Vaccination Against the Novel COVID-19 Infection in the Russian Federation: The Role of Mental Health, and Personal and Social Issues as Targets for Future Psychosocial Interventions.

Background: Vaccine hesitancy causes serious difficulties in vaccination campaigns in many countries. The study of the population's attitude toward vaccination and detection of the predictive important individual psychological and social factors defining the vaccination necessity perception will allow elaborating promoting vaccination adherence measures. Objectives: The aim of this research was to study COVID-19 threat appraisal, fear of COVID-19, trust in COVID-19 information sources, COVID-19 conspiracy beliefs, and the relationship of sociodemographic variables to COVID-19 preventive behavior. Methods: We carried out a cohort cross-sectional study of the population's attitude toward vaccination against the novel COVID-19 coronavirus infection, using a specially designed questionnaire for an online survey. Totally, there were 4,977 respondents, ranging in age from 18 to 81 years. Statistical assessment was carried out using the SPSS-11 program. Results: There were different attitudes toward vaccination. Among respondents, 34.2% considered vaccination to be useful, 31.1% doubted its effectiveness, and 9.9% considered vaccination unnecessary. The survey indicated that 7.4% of respondents were indifferent to the vaccine, while 12.2% deemed it to be dangerous. Nearly one-third (32.3%) of respondents indicated that they did not plan to be vaccinated, while another third (34.0%) would postpone their decision until more comprehensive data on the results and effectiveness of vaccination were available. Only 11.6% of the respondents were vaccinated at the time of the study. Young people were less focused on vaccination compared to middle-aged and elderly people. Receiving information concerning COVID-19 vaccination from healthcare workers and scientific experts was associated with greater vaccination acceptance. Conclusion: The study results showed that vaccination attitudes interacted with individuals' mental health and various sociodemographic factors. Insofar as reports of physicians and experts are essential for shaping attitudes to vaccination, the study results inform the selection of target groups in need of particular psychosocial interventions to overcome their vaccine hesitancy.