Lighting intensity and photoperiod serves tailoring nitrate assimilation indices in red and green baby leaf lettuce.

BACKGROUND: Understanding plant responses to light quantity in indoor horticultural systems is important for optimising lettuce growth and metabolism as well as energy utilisation efficiency. Light intensity and photoperiod sufficient for normal plant growth parameters might be not efficient for nitrate assimilation. Therefore, this study explored and compared the effects of different light intensities (100-500 µmol m-2 s-1 ) and photoperiods (12-24 h) on the growth and nitrate assimilation in red and green leaf lettuce (Lactuca sativa L.). RESULTS: For efficient nitrate assimilation, 300-400 µmol m-2 s-1 photosynthetic photon flux density (PPFD) and 16-18 h photoperiod is necessary for red and green lettuces. The insufficient light quantity resulted in reduced growth and remarkable increase in nitrate and nitrite contents in both cultivars. Short photoperiods, similarly to low PPFD, growth parameters, chlorophyll indices and nitrate assimilation indices showed the shortage of photosynthetic products for normal plant physiological processes. Short photoperiods had the least pronounced effect on nitrate and nitrite contents in lettuce leaves. CONCLUSION: Light intensity was superior compared to photoperiods for efficient nitrate assimilation in both lettuce cultivars. Under short photoperiods, similarly to low intensity, growth parameters, chlorophyll index and nitrate assimilation indices showed a shortage of photosynthetic products for normal physiological processes. The free amino acid concentration increased, but it was not efficiently incorporated in proteins, as their level in lettuce was lower compared to those for moderate photoperiods. © 2019 Society of Chemical Industry.

The Effect of Ripening Stages on the Accumulation of Polyphenols and Antioxidant Activity of the Fruit Extracts of Solanum Species.

The aim of the research was to evaluate the influence of the ripening stage on the accumulation of polyphenols and antioxidant activity in fruits of Solanum species. The experiment included two factors: I-four different Solanum species (S. melanocerasum, S. nigrum, S. villosum, and S. retroflexum) and II-three ripening stages. High-performance liquid chromatography (HPLC) was used to analyze the individual phenolic compounds (flavonoids and phenolic acids), and the spectrophotometric method was applied to determine antioxidant activity. The results revealed that the accumulation of polyphenols and antioxidant activity in fruits of Solanum species depends on the stage of ripening and species. All studied Solanum species fruits had the highest content of total phenolic acid at ripening stage III and the greatest antioxidant activity at ripening stage I. Fully ripe fruits of S. melanocerasum contained significantly more total flavonoids, whereas S. nigrum contained significantly more total phenolic acids than other investigated Solanum species fruits. The significantly highest antioxidant activity was found in S. melanocerasum fruits at ripening stage I.

Changes in Pomological and Physical Parameters in Rosehips during Ripening.

Rosehips of various Rosa spp. are well known for having human health-promoting compounds like mineral nutrients, vitamins, fatty acids, and phenolic compounds. However, little is known about rosehip characteristics which describe the fruit quality and may indicate appropriate harvest times. Our study evaluated the pomological (width, length, and weight of fruits, flesh weight, and seed weight), texture, and CIE colour parameters (L*, a*, and b*), chroma (C), and hue angle (h°) of rosehip fruits of Rosa canina, Rosa rugosa, and genotypes of Rosa rugosa 'Rubra' and 'Alba', harvested at five ripening stages (I-V). The main results revealed that genotype and ripening stage significantly affected parameters. The significantly longest (R. canina) and widest fruits (R. Rugosa) were measured at ripening stage V. Genotypes of R. rugosa 'Rubra' and 'Alba' had significantly higher fruit and flesh weights at ripening stage V. Rosehips of all investigated genotypes expressed darkness (lower L*) during ripening, and had the highest hue angle h° values at ripening stage I while the lowest was at stage V. The significantly lowest skin elasticity of rosehips was found at stage V. However, R. canina was distinguished by the highest fruit skin elasticity and strength. As our results show, the desired pomological, colour, and texture features of various species and cultivars rosehips can be optimised according to the harvest time.

Vegetation Indices for Early Grey Mould Detection in Lettuce Grown under Different Lighting Conditions.

Early detection of pathogenic fungi in controlled environment areas can prevent major food production losses. Grey mould caused by Botrytis cinerea is often detected as an infection on lettuce. This paper explores the use of vegetation indices for early detection and monitoring of grey mould on lettuce under different lighting conditions in controlled environment chambers. The aim was focused on the potential of using vegetation indices for the early detection of grey mould and on evaluating their changes during disease development in lettuce grown under different lighting conditions. The experiment took place in controlled environment chambers, where day/night temperatures were 21 ± 2/17 ± 2 °C, a 16 h photoperiod was established, and relative humidity was 70 ± 10% under different lighting conditions: high-pressure sodium (HPS) and light-emitting diode (LED) lamps. Lettuces were inoculated by 7-day-old fungus Botrytis cinerea isolate at the BBCH 21. As a control, non-inoculated lettuces were grown under HPS and LEDs (non-inoculated). Then, the following were evaluated: Anthocyanin Reflectance Index 2 (ARI2); Carotenoid Reflectance Index 2 (CRI2); Structure Intensive Pigment Index (SIPI); Flavanol Reflectance Index (FRI); Greenness (G); Greenness 2 (G2); Redness (R); Blue (B); Blue Green Index 2 (BGI2); Browning Index 2 (BRI2); Lichtenthaler Index 1 (LIC1); Pigment Specific Simple Ratio (PSSRa and PSSRb); Gitelson and Merzlyak (GM1 and GM2); Zarco Tejada-Miller Index (ZMI); Normalized Difference Vegetation Index (NDVI); Simple Ratio (SR); Red-Eye Vegetation Stress Index (RVSI); Photochemical Reflectance Index (PRI); Photochemical Reflectance Index 515 (PRI515); Water Band Index (WBI); specific disease index for individual study (fD); Healthy Index (HI); Plant Senescence Reflectance (PSRI); Vogelmann Red Edge Index (VREI1); Red Edge Normalized Difference Vegetation Index (RENDVI); and Modified Red Edge Simple Ratio (MRESRI). Our results showed that the PSRI and fD vegetation indices significantly detected grey mould on lettuce grown under both lighting systems (HPS and LEDs) the day after inoculation. The results conclusively affirmed that NDVI, PSRI, HI, fD, WBI, RVSI, PRI, PRI515, CRI2, SIPI, chlorophyll index PSSRb, and coloration index B were identified as the best indicators for Botrytis cinerea infection on green-leaf lettuce (Lactuca sativa L. cv Little Gem) at the early stage of inoculated lettuce's antioxidative response against grey mould with a significant increase in chlorophyll indices.

Different LED Light Wavelengths and Photosynthetic Photon Flux Density Effect on Colletotrichum acutatum Growth.

The study aimed to evaluate the effect of different photon flux density (PFD) and light-emitting diodes (LED) wavelengths on strawberry Colletotrichum acutatum growth characteristics. The C. acutatum growth characteristics under the blue 450 nm (B), green 530 nm (G), red 660 nm (R), far-red 735 nm (FR), and white 5700 K (W) LEDs at PFD 50, 100 and 200 µmol m-2 s-1 were evaluated. The effect on C. acutatum mycelial growth evaluated by daily measuring until five days after inoculation (DAI). The presence of conidia and size (width and length) evaluated after 5 DAI. The results showed that the highest inhibition of fungus growth was achieved after 1 DAI under B and G at 50 µmol m-2 s-1 PFD. Additionally, after 1-4 DAI under B at 200 µmol m-2 s-1 PFD. The lowest conidia width was under FR at 50 µmol m-2 s-1 PFD and length under FR at 100 µmol m-2 s-1 PFD. Various LED light wavelengths influenced differences in C. acutatum colonies color. In conclusion, different photosynthetic photon flux densities and wavelengths influence C. acutatum growth characteristics. The changes in C. acutatum morphological and phenotypical characteristics could be related to its ability to spread and infect plant tissues. This study's findings could potentially help to manage C. acutatum by LEDs in controlled environment conditions.

The Influence of End-of-Day Blue Light on the Growth, Photosynthetic, and Metabolic Parameters of Lettuce at Different Development Stages.

This study evaluates the effect of end-of-day blue (EOD B) light on the physiological response of lettuce (Lactuca sativa, Lobjoits Green Cos) at different phenological development stages. Plants were grown in a controlled environment growth chamber (day/night temperature 21 ± 2 °C; relative air humidity 60 ± 5%) under the light of light-emitting diodes (LEDs) consisting of 5% blue (B; 450 nm), 85% red (R; 660 nm), and 10% green (G; 530 nm) photosynthetic photon flux density (PPFD) at 200 µmol m-2 s-1 for 16 h d-1 (BRG, control) for 8, 15, and 25 days (BBCH 12, BBCH 14, and BBCH 18, respectively). For the EOD B treatments, lettuce plants were additionally illuminated with 100% of B light at 30 and 60 µmol m-2 s-1 PPFD for 4 h d-1 (B30 and B60, respectively). The results show that EOD B light caused the elevated shoot elongation of lettuce plants regardless of their growth stages. However, leaf width increased only in more developed lettuce plants (BBCH 18). EOD B light negatively affected the development of new leaves and fresh weight, except for seedlings (BBCH 12). Most photosynthetic and spectral leaf indices also decreased when lettuce was treated with EOD B light, especially under the PPFD level of 60 µmol m-2 s-1. Moreover, the changes in metabolic parameters such as DPPH free radical activity, free proline content, and H+-ATPase activity in lettuce showed a plant response to unfavorable conditions to EOD B light.

Defense response of strawberry plants against Botrytis cinerea influenced by coriander extract and essential oil.

Essential oils and extracts are investigated in sustainable plant protection area lately. Alternative antifungal substances are especially relevant for major economic-relevance pathogens, like Botrytis cinerea (causal agent of strawberry grey mold), control. However, the reaction of plants to alternative protection with plant-origin products is currently unknown. Induced stress in plants causes changes in antioxidant and photosynthetic systems. The aim of the research was to determine the defense response of strawberry plants under application of coriander seed products. In the first step of the research, we determined coriander seed (Coriandrum sativum), black seed (Nigella sativa) and peppermint leaf (Menta × piperita) products' antifungal activity against B. cinerea in vitro. Secondly, we continued evaluation of antifungal activity under controlled environment on strawberry plants of the most effective coriander seed products. Additionally, we evaluated the antioxidant and photosynthetic parameters in strawberries, to examine the response of plants. Antifungal activity on strawberries was determined based on grey mold incidence and severity after application of coriander products. Impact on photosynthetic system was examined measuring photosynthetic rate, transpiration rate, stomatal conductance, and intercellular to ambient CO2 concentration. Strawberry leaves were collected at the end of the experiment to analyze the antioxidant response. The highest antifungal activity both in vitro and on strawberries had coriander seed essential oil, which decreased grey mold severity. Coriander extract increased the photosynthetic capacity and antioxidant response of strawberry plants, however had negative effect on suppression of grey mold. In most cases, the essential oil activated antioxidant response of strawberry plants lower than extract. Our study results provide no direct impact of increased photosynthetic capacity values and antifungal effect after treatment with natural oils. The highest concentrations of coriander essential oil and extract potentially demonstrated a phytotoxic effect.

Phenolic Compounds Content Evaluation of Lettuce Grown under Short-Term Preharvest Daytime or Nighttime Supplemental LEDs.

The study aimed to determine the changes in phenolic compounds content in lettuce (Lactuca sativa L. cv. Little Gem) depending on the preharvest short-term daytime or nighttime supplemental light-emitting diodes (LEDs) to high-pressure sodium lamps (HPS) lighting in a greenhouse during autumn and spring cultivation. Plants were grown in a greenhouse under HPS supplemented with 400 nm, 455 nm, 530 nm, 455 + 530 nm or 660 nm LEDs light for 4 h five days before harvest. Two experiments (EXP) were performed: EXP1-HPS, and LEDs treatment during daytime 6 PM-10 PM, and EXP2-LEDs treatment at nighttime during 10 AM-2 PM. LEDs' photosynthetic photon flux density (PPFD) was 50 and HPS-90 ± 10 µmol m-2 s-1. The most pronounced positive effect on total phenolic compounds revealed supplemental 400 and 455 + 530 nm LEDs lighting, except its application during the daytime at spring cultivation, when all supplemental LEDs light had no impact on phenolics content variation. Supplemental 400 nm LEDs applied in the daytime increased chlorogenic acid during spring and chicoric acid during autumn cultivation. 400 nm LEDs used in nighttime enhanced chlorogenic acid accumulation and rutin during autumn. Chicoric and chlorogenic acid significantly increased under supplemental 455 + 530 nm LEDs applied at daytime in autumn and used at nighttime-in spring. Supplemental LEDs application in the nighttime resulted in higher phenolic compounds content during spring cultivation and the daytime during autumn cultivation.

Regulation of the Photon Spectrum on Growth and Nutritional Attributes of Baby-Leaf Lettuce at Harvest and during Postharvest Storage.

The photon flux density (PFD) and spectrum regulate the growth, quality attributes, and postharvest physiology of leafy vegetables grown indoors. However, limited information is available on how a photon spectrum enriched with a broad range of different wavebands regulates these factors. To determine this, we grew baby-leaf lettuce 'Rouxai' under a PFD of 200 µmol m-2 s-1 provided by warm-white (WW; control) light-emitting diodes (LEDs) supplemented with either 30 µmol m-2 s-1 of ultraviolet-A (+UV30) or 50 µmol m-2 s-1 of blue (+B50), green (+G50), red (+R50), or WW (+WW50) light. We then quantified growth attributes and accumulated secondary metabolites at harvest and during storage in darkness at 5 °C. Additional +G50 light increased shoot fresh and dry weight by 53% and 59% compared to the control. Relative chlorophyll concentration increased under +UV30, +G50, and especially +B50. At harvest, +B50 increased total phenolic content (TPC) by 25% and anthocyanin content (TAC) by 2.0-fold. Additionally, +G50 increased antiradical activity (DPPH) by 29%. After each day of storage, TPC decreased by 2.9 to 7.1% and DPPH by 3.0 to 6.2%, while TAC degradation was less pronounced. Principal component analysis indicated a distinct effect of +G50 on the lettuce at harvest. However, concentrations of metabolites before and during storage were usually greatest under the +B50 and +R50 treatments.

The Effect of Monochromatic LED Light Wavelengths and Photoperiods on Botrytis cinerea.

Botrytis cinerea is a ubiquitous necrotrophic pathogen causing grey mould in economically important crops. Light effect in horticulture is undeniable and fungi also react to light. Selected specific light-emitting diodes (LEDs) and photoperiods can be used for fungal pathogen inhibition. This study aimed to evaluate how LED light wavelengths and photoperiods affect the growth parameters of B. cinerea. The morphological (mycelium appearance, sclerotia distribution) and phenotypic (conidia presence and size, mycelium growth rate, recovery) characteristics of the fungal pathogen B. cinerea were evaluated under royal blue 455 nm, blue 470 nm, cyan 505 nm, yellow 590 nm, and red 627 nm LED lights at various photoperiods (4, 8, 12, 16, 20, 24 h). The results revealed that the light conditions and photoperiods influenced the B. cinerea morphological and phenotypic characteristics. Overall, the highest B. cinerea inhibition was under yellow (590 nm) LED light at 4 and 8 h photoperiods. Conidia did not form under blue 455 nm at 8, 16, 20, and 24 h photoperiods. Therefore, it can be assumed that the phenotypic and morphological features of B. cinerea depend on the specific photoperiod and LED light wavelength. The results allowed an exploration of original research approaches, raised new scientific questions for further investigation, and suggested new green plant protection solutions.

Effect of Different Ratios of Blue and Red LED Light on Brassicaceae Microgreens under a Controlled Environment.

The consumption of microgreens has increased due to their having higher levels of bioactive compounds and mineral nutrients than mature plants. The lighting conditions during the cultivation of microgreens, if optimally selected, can have a positive effect by further increasing their nutritional value. Thus, our study aimed to determine the changes in mineral nutrients contents of Brassicaceae microgreens depending on different blue-red (B:R) light ratios in light-emitting diode (LED) lighting and to evaluate their growth and nutritional value according to different indexes. Experiments were performed in controlled environment growth chambers at IH LRCAF, 2020. Microgreens of mustard (Brassica juncea 'Red Lace') and kale (Brassica napus 'Red Russian') were grown hydroponically under different B:R light ratios: 0%B:100%R, 10%B:90%R, 25%B:75%R, 50%B:50%R, 75%B:25%R, and 100%B:0%R. A 220 µmol m-2 s-1 total photon flux density (TPFD), 18 h photoperiod, 21/17 ± 2 °C temperature and 60% ± 5% relative humidity in the growth chamber were maintained during cultivation. We observed that an increasing percentage of blue light in the LED illumination spectrum during growth was associated with reduced elongation in the microgreens of both species and had a positive effect on the accumulation of mostly macro- and micronutrients. However, different B:R light ratios indicate a species-dependent response to changes in growth parameters such as leaf area, fresh and dry mass, and optical leaf indexes such as for chlorophyll, flavonol, anthocyanin, and carotenoid reflectance.

The distinct impact of multi-color LED light on nitrate, amino acid, soluble sugar and organic acid contents in red and green leaf lettuce cultivated in controlled environment.

In this study we explore the effects of multi-colour LED lighting spectrum on nutritive primary metabolites in green ('Lobjoits green cos') and red ('Red cos') leaf lettuce (Lactuca sativa L.), cultivated in controlled environment. The basal lighting, consisting of blue 455 nm, red 627 and 660 nm and far red 735 nm LEDs, was supplemented with UV-A 380 nm, green 510 nm, yellow 595 nm or orange 622 nm LED wavelengths at total photosynthetic photon flux density of 300 µmol m-2 s-1. Supplemental lighting colours did not affect lettuce growth; however had distinct impact on nitrite, amino acid, organic acid, and soluble sugar contents. Orange, green and UV-A light had differential effects on red and green leaf lettuce metabolism and interplay with nutritional value and safety of lettuce production. The metabolic response was cultivar specific; however green light had reasonable impact on the contents of nutritive primary metabolites in red and green leaf lettuce.

Nutrient Levels in Brassicaceae Microgreens Increase Under Tailored Light-Emitting Diode Spectra.

To increase the nutritional value and levels of essential minerals in vegetable food, microgreens are promising targets. The metabolic processes of microgreens can be managed with different cultivation techniques, which include manipulating the properties of light derived by light-emitting diodes (LEDs). In this study Brassicaceae microgreens (kohlrabi Brassica oleracea var. gongylodes, broccoli Brassica oleracea, and mizuna Brassica rapa var. Japonica) were cultivated under different light spectral quality, and the metabolic changes insoluble sugars (hexoses and sucrose), ascorbic acid, ß-carotene, and contents of non-heme iron (Fe) and its connection with magnesium (Mg) or calcium (Ca) levels were monitored. Plants grew under the primary LED light spectrum (the combination of blue light at 447 nm, red at 638 and 665 nm, and far-red at 731 nm) or supplemented with LED green light at 520 nm, yellow at 595 nm, or orange at 622 nm. The photoperiod was 16 h, and a total PPFD of 300 µmol m-2 s-1 was maintained. Under supplemental yellow light at 595 nm, the content of soluble carbohydrates increased significantly in mizuna and broccoli. Under all supplemental light components, ß-carotene accumulated in mizuna, and ascorbic acid accumulated significantly in kohlrabi. Under supplemental orange light at 622 nm, Fe, Mg, and Ca contents increased significantly in all microgreens. The accumulation of Fe was highly dependent on promoters and inhibitors of Fe absorption, as demonstrated by the very strong positive correlations between Fe and Ca and between Fe and Mg in kohlrabi and broccoli, and the strong negative correlations between Fe and ß-carotene and between Fe and soluble carbohydrates in kohlrabi. Thus, the metabolic changes that occurred in treated microgreens led to increases in the contents of essential nutrients. Therefore, selected supplemental LED wavelengths can be used in the cultivation of Brassicaceae microgreens to preserve and increase the contents of specific nutritionally valuable metabolites.

Response of Mustard Microgreens to Different Wavelengths and Durations of UV-A LEDs.

Ultraviolet A (UV-A) light-emitting diodes (LEDs) could serve as an effective tool for improving the content of health-promoting bioactive compounds in plants in controlled-environment agriculture (CEA) systems. The goal of this study was to investigate the effects of UV-A LEDs at different wavelengths (366, 390, and 402 nm) and durations (10 and 16 h) on the growth and phytochemical contents of mustard microgreens (Brassica juncea L. cv. "Red Lion"), when used as supplemental light to the main LED lighting system (with peak wavelengths of 447, 638, 665, and 731 nm). Plants were grown for 10 days under a total photon flux density (TPFD) of 300 µmol m-2 s-1 and 16-h light/8-h dark period. Different UV-A wavelengths and irradiance durations had varied effects on mustard microgreens. Supplemental UV-A radiation did not affect biomass accumulation; however, the longest UV-A wavelength (402 nm) increased the leaf area of mustard microgreens, regardless of the duration of irradiance. The concentration of the total phenolic content and α-tocopherol mostly increased under 402-nm UV-A, while that of nitrates increased under 366- and 390-nm UV-A at both radiance durations. The contents of lutein/zeaxanthin and ß-carotene increased in response to the shortest UV-A wavelength (366 nm) at 10-h irradiance as well as longer UV-A wavelength (390 nm) at 16 h irradiance. The most positive effect on the accumulation of mineral elements, except iron, was observed under longer UV-A wavelengths at 16-h irradiance. Overall, these results suggest that properly composed UV-A LED parameters in LED lighting systems could improve the nutritional quality of mustard microgreens, without causing any adverse effects on plant growth.