Nanoparticle-mediated cancer cell therapy: basic science to clinical applications.

Every sixth person in the world dies due to cancer, making it the second leading severe cause of death after cardiovascular diseases. According to WHO, cancer claimed nearly 10 million deaths in 2020. The most common types of cancers reported have been breast (lung, colon and rectum, prostate cases), skin (non-melanoma) and stomach. In addition to surgery, the most widely used traditional types of anti-cancer treatment are radio- and chemotherapy. However, these do not distinguish between normal and malignant cells. Additional treatment methods have evolved over time for early detection and targeted therapy of cancer. However, each method has its limitations and the associated treatment costs are quite high with adverse effects on the quality of life of patients. Use of individual atoms or a cluster of atoms (nanoparticles) can cause a paradigm shift by virtue of providing point of sight sensing and diagnosis of cancer. Nanoparticles (1-100 nm in size) are 1000 times smaller in size than the human cell and endowed with safer relocation capability to attack mechanically and chemically at a precise location which is one avenue that can be used to destroy cancer cells precisely. This review summarises the extant understanding and the work done in this area to pave the way for physicians to accelerate the use of hybrid mode of treatments by leveraging the use of various nanoparticles.

Probiotic potential of Streptomyces levis strain HFM-2 isolated from human gut and its antibiofilm properties against pathogenic bacteria.

BACKGROUND: Antimicrobial resistance (AMR) is a serious worldwide public health concern that needs immediate action. Probiotics could be a promising alternative for fighting antibiotic resistance, displaying beneficial effects to the host by combating diseases, improving growth, and stimulating the host immune responses against infection. This study was conducted to evaluate the probiotic, antibacterial, and antibiofilm potential of Streptomyces levis strain HFM-2 isolated from the healthy human gut. RESULTS: In vitro antibacterial activity in the cell-free supernatant of S. levis strain HFM-2 was evaluated against different pathogens viz. K. pneumoniae sub sp. pneumoniae, S. aureus, B. subtilis, VRE, S. typhi, S. epidermidis, MRSA, V. cholerae, M. smegmatis, E. coli, P. aeruginosa and E. aerogenes. Further, the ethyl acetate extract from S. levis strain HFM-2 showed strong biofilm inhibition against S. typhi, K. pneumoniae sub sp. pneumoniae, P. aeruginosa and E. coli. Fluorescence microscopy was used to detect biofilm inhibition properties. MIC and MBC values of EtOAc extract were determined at 500 and 1000 µg/mL, respectively. Further, strain HFM-2 showed high tolerance in gastric juice, pancreatin, bile, and at low pH. It exhibited efficient adhesion properties, displaying auto-aggregation (97.0%), hydrophobicity (95.71%, 88.96%, and 81.15% for ethyl acetate, chloroform and xylene, respectively), and showed 89.75%, 86.53%, 83.06% and 76.13% co-aggregation with S. typhi, MRSA, S. pyogenes and E. coli, respectively after 60 min of incubation. The S. levis strain HFM-2 was susceptible to different antibiotics such as tetracycline, streptomycin, kanamycin, ciprofloxacin, erythromycin, linezolid, meropenem, amikacin, gentamycin, clindamycin, moxifloxacin and vancomycin, but resistant to ampicillin and penicillin G. CONCLUSION: The study shows that S. levis strain HFM-2 has significant probiotic properties such as good viability in bile, gastric juice, pancreatin environment, and at low pH; proficient adhesion properties, and antibiotic susceptibility. Further, the EtOAc extract of Streptomyces levis strain HFM-2 has a potent antibiofilm and antibacterial activity against antibacterial-resistant clinical pathogens.

Biosynthesis of silver nanoparticle from flower extract of Dillenia indica and its efficacy as antibacterial and antioxidant.

Dillenia indica is a medicinal tree of the Dilleniaceae and its flower extract was used for the synthesis of silver nanoparticle (AgNPs). The optimal conditions for AgNPs synthesis were as such: 2 mM AgNO3, pH 4.5 and 48-h reaction time. The characteristic band of AgNPs was observed at the wavelength of 435 nm by UV-visible spectroscopic study. Fourier-transform infrared (FTIR) analysis depicted the involvement of several functional groups of plant extracts in the synthesis of AgNPs. Nanoparticles were mostly spherical shaped and uniformly distributed, when observation was made by Transmission electron microscopy (TEM). Energy Dispersive X-Ray (EDX) showed absorption peak approximately at 3 keV thus confirmed the presence of silver metal in AgNP. X-ray diffraction (XRD) investigation and selected area electron diffraction (SAED) patterns showed the crystalline nature of the AgNPs. Dynamic light scattering (DLS) analysis exhibited average size of the nanoparticles as 50.17 nm with a polydispersity index (PDI) value of 0.298. The zeta potential of nanoparticles was observed as -24.9 mV. To assess antibacterial activity, both AgNPs alone or its combination with the antibiotic were tried against six pathogenic bacteria. The combination of AgNPs with antibiotic was maximum effective against Shigella boydii (16.07 ± 0.35) and Klebsiella pneumoniae (15.03 ± 0.20). AgNPs alone showed maximum inhibition for both Gram-positive bacteria: methicillin-resistant Staphylococcus aureus (19.97 ± 0.20 mm) and Enterococcus faecium (19.80 ± 0.15 mm). Maximum inhibition of Enterobactor cloacae and Pseudomonas aeruginosa was observed by antibiotic taken alone. Evaluation through 2,2-diphenyl-1-picrylhydrazyl (DPPH) and DNA nicking assays demonstrated the antioxidant capabilities of the nanoparticles.

Nanomaterials for diabetes: diagnosis, detection and delivery.

537 million people worldwide suffer from diabetes mellitus, a problem of glucose management that is related to a number of major health risks, including cardiovascular diseases. There is a need for new, efficient formulations of diabetic medications to address this condition and its related consequences because existing treatments have a number of drawbacks and limits. This encouraged the development of treatment plans to get around some of these restrictions, like low therapeutic drug bioavailability or patients' disobedience to existing therapies. Approaches based on nanotechnology have a lot of promise to enhance the treatment of diabetic patients. In order to manage blood glucose, this review article highlights recent developments and explores the potential applications of different materials (polymeric, ceramic, dendrimers, etc.) as nanocarriers for the delivery of insulin and other antidiabetic medications. Using an injectable and acid-degradable polymeric network produced by the electrostatic interaction of oppositely charged dextran nanoparticles loaded with insulin and glucose-specific enzymes, we reviewed a glucose-mediated release approach for the self-regulated delivery of insulin, in which, after a degradable nano-network was subcutaneously injected into type 1 diabetic mice,in vivoexperiments confirmed that these formulations improved glucose management. In addition, a discussion of silica-based nanocarriers, their potential for treating diabetes and controlling blood glucose levels, and an explanation of the role of dendrimers in diabetes treatment have been covered. This is done by utilizing the properties of silica nanoparticles, such as their tuneable particle and pore size, surface chemistry, and biocompatibility. The article summarized the significance of nanomaterials and their uses in the diagnosis and treatment of diabetes overall, illuminating the field's potential and outlining its prospects for the future.

Retraction: Metal-ion batteries for electric vehicles: current state of the technology, issues and future perspectives.

[This retracts the article DOI: 10.1039/D1NA00214G.].

Comparative Evaluation of Horizontal Lip Position With Ricketts Esthetic Plane in Preschool and School-Going Children of Bankura, West Bengal: A Craniofacial Anthropometric Study.

Introduction The horizontal lip position and esthetic plane are two important parameters to define facial beauty, and these factors are always given importance in children undergoing fixed orthodontic therapy. The purpose of this study was to evaluate horizontal lip position in primary and mixed dentition children with class I occlusion and to analyze its association with gender among preschool and schoolchildren of villages in the Bankura district in West Bengal. Materials and methods Researchers screened 437 children for the study and selected those who met the inclusion criteria. A total of 407 children were segregated: 201 children aged three to five years with the flush terminal plane and mesial step in primary teeth and 206 children aged seven to eleven years with class I occlusion in mixed dentition were selected from schools in villages in Bankura district, West Bengal, India. The subjects were instructed to hold the head in the natural head position by looking straight, and points were marked on the nose and chin tip, respectively. A metallic ruler was placed from nose to chin, representing Rickett's esthetic line. The horizontal lip distance to the esthetic plane of both upper and lower lips was measured as a linear distance from the most anterior part of the lip to the metallic ruler. The data were recorded, compared with gender, and statistically analyzed using the Chi-square test using the Statistical Package of Social Sciences software (SPSS version 19.0, 2015, IBM Corp., Armonk, NY). Results The most prevalent horizontal lip distance for both upper and lower lips to the esthetic line for primary and mixed dentition in the Bankura region of West Bengal was category I, where the lip is seen beyond the E plane, followed by category II, where lips are at a horizontal distance in the range of 0-1.5 mm from the esthetic line. A significant correlation of lip position with Rickets aesthetic plane was also illustrious with gender in primary dentition, but a non-significant association with gender was noted in mixed dentition. Conclusion Children with class I occlusion from the Bankura district of West Bengal showed a higher percentage of lip position beyond the esthetic plane in both primary and mixed dentition, which is not in line with the inference of Ricketts's studies on the Caucasian population. There is a definite association between gender and horizontal lip distance in Ricketts esthetic plane. Protrusive upper lips were seen more in males, and retrusive lips were seen more in females. Clinical significance  The horizontal lip position with reference to Ricketts esthetic plane has been documented in the literature for adults and teens undergoing fixed orthodontic treatment. However, there is no study done to define these measurements in preschool and school-going children, which can assist in determining future esthetic profiles and in preparing a protocol for early age interceptive orthodontics along with aesthetic rehabilitation of the anterior area of the mouth.

Synergistic Coassembly of Folic Acid-Based Supramolecular Polymer with a Covalent Polymer Toward Fabricating Functional Antibacterial Biomaterials.

Supramolecular biomaterials can recapitulate the structural and functional facets of the native extracellular matrix and react to biochemical cues, leveraging the unique attributes of noncovalent interactions, including reversibility and tunability. However, the low mechanical properties of supramolecular biomaterials can restrict their utilization in specific applications. Combining the advantages of supramolecular polymers with covalent polymers can lead to the fabrication of tailor-made biomaterials with enhanced mechanical properties/degradability. Herein, we demonstrate a synergistic coassembled self-healing gel as a multifunctional supramolecular material. As the supramolecular polymer component, we chose folic acid (vitamin B9), an important biomolecule that forms a gel comprising one-dimensional (1D) supramolecular polymers. Integrating polyvinyl alcohol (PVA) into this supramolecular gel alters its ultrastructure and augments its mechanical properties. A drastic improvement of complex modulus (G*) (∼3674 times) was observed in the folic acid-PVA gel with 15% w/v PVA (33215 Pa) compared with the folic acid gel (9.04 Pa). The coassembled hydrogels possessed self-healing and injectable/thixotropic attributes and could be printed into specific three-dimensional (3D) shapes. Synergistically, the supramolecular polymers of folic acid also improve the toughness, durability, and ductility of the PVA films. A nanocomposite of the gels with silver nanoparticles exhibited excellent catalytic efficiency and antibacterial activity. The folic acid-PVA coassembled gels and films also possessed high cytocompatibility, substantiated by the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) and live-dead assays. Taken together, the antibacterial and cell-adhesive attributes suggest potential applications of these coassembled biomaterials for tissue engineering and wound healing.

Antioxidant and chemoprotective potential of Streptomyces levis strain isolated from human gut.

In the current study, Streptomyces levis strain HFM-2 has been isolated from healthy human gut. Streptomyces sp. HFM-2 was identified based on the polyphasic approach that included cultural, morphological, chemotaxonomical, phylogenetic, physiological, and biochemical characteristics. 16S rRNA gene sequence of strain HFM-2 exhibited 100% similarity with Streptomyces levis strain 15423 (T). The EtOAc extract of Streptomyces levis strain HFM-2 showed potential antioxidant activity, along with 69.53 ± 0.19%, 64.76 ± 0.13%, and 84.82 ± 0.21% of scavenging activity for ABTS, DPPH, and superoxide radicals, respectively at 600 µg/mL. The IC50 values i.e. 50% scavenging activity for DPPH, ABTS, and superoxide radicals were achieved at 497.19, 388.13, and 268.79 (µg/mL), respectively. The extract's reducing power and total antioxidant capacity were determined to be 856.83 ± 0.76 and 860.06 ± 0.01 µg AAE/mg of dry extract, respectively. In addition, the EtOAc extract showed protection against DNA damage from oxidative stress caused by Fenton's reagent, and cytotoxic activity against HeLa cervical cancer, Skin (431) cancer, Ehrlich-Lettre Ascites-E (EAC) carcinoma, and L929 normal cell lines. The IC50 values against HeLa, 431 skin, and EAC carcinoma cell lines were found to be 50.69, 84.07, and 164.91 µg/mL, respectively. The EtOAc extract showed no toxicity  towards L929 normal cells. In addition, flow cytometric analysis exhibited reduced mitochondrial membrane potential (MMP), and enhanced levels of reactive oxygen species (ROS). The EtOAc extract was chemically analyzed using GCMS to determine the components executing its bioactivities.

Fabrication and testing of a multifunctional SiO2@ZnO core-shell nanospheres incorporated polymer coating for sustainable marine transport.

We report the development of a coating system relying on the incorporation of SiO2@ZnO core-shell nanospheres in polyurethane media as a novel approach to achieve longevity and sustainability in marine transport. This polymeric coating showed significant improvement in surface abrasion resistance, the transition from a hydrophilic state to a hydrophobic state (~ 125.2° ± 2°), improved antifungal, antibacterial and antialgae effects which make the proposed coating ideal to protect steel surfaces against biofouling. To substantiate our claims, we performed X-ray diffraction, Transmission electron microscopy, Fourier transform infrared spectroscopy, scanning acoustic microscopy, Thermogravimetric analysis (TGA), contact angle measurements, antimicrobial (antialgal, antibacterial, antifungal) tests and Taber abrasion tests (ASTM D1044 and D4060) to highlight the mechanical and biological functionality as well as the bonding configuration of this coating. The wear analysis of the Taber abraded coating using SEM and optical microscopy showed significant improvement in the adhesion and shear resistance achieved by the SiO2@ZnO core-shell nanospheres incorporated PU coating which was a contrasting feature compared to using PU alone. The overall investigations we performed led us to find out that the addition of 4% (wt.) SiO2@ZnO core-shell nanoparticles to the PU media deposited on the low carbon steel surface demonstrated remarkable antimicrobial performance with almost no bacterial growth, significant reductions in growth for algae to about 90% and fungus to about 95%.

Insecticidal potential of endophytic Streptomyces sp. against Zeugodacus cucurbitae (Coquillett) (Diptera: Tephritidae) and biosafety evaluation.

Fruit flies of Tephritidae family pose a serious threat to cultivation of fruits and vegetables across the world. Among them, melon fruit fly, Zeugodacus cucurbitae (Coquillett) (Diptera: Tephritidae) is a devastating pest of plants from Cucurbitaceae family. In a rising concern about the harmful effects associated with the use of chemical insecticides and development of resistance in pest insects, safer pest management strategies such as, use of biopesticides of microbial origin are being contemplated. Therefore, the present study aimed to evaluate the insecticidal potential of Streptomyces sp. SP5 protein extract against Z. cucurbitae. MTT assay, Ames mutagenicity, DNA nicking, and comet assay were conducted to determine the biosafety of protein extract. Second instar larvae of Z. cucurbitae were treated with various concentrations (1, 100, 200, 300, 400, and 500 µg/ml) of Streptomyces sp. SP5 protein extract. The protein extract showed significant larvicidal effects with LC50 value of 308.92 µg/ml. The percentage of adults emerged declined with increase in concentration. There was significant prolongation in developmental durations of the larvae. Various morphological aberrations in the form of deformed adults and pupae and decline in pupal weight were also observed. The nutritional physiology of the treated larvae was also adversely affected. The results from biosafety evaluation revealed antimutagenic and non-toxic nature of Streptomyces sp. proteins. This study indicates that Streptomyces sp. SP5 has the potential to be used as an ecologically safe biocontrol agent against Z. cucurbitae.

"Triple Tooth Synodontia" of Primary Incisors in Triangular Configuration: A Rare Aberrant Case Report.

Aim: This case report aims to present triple tooth synodontia of primary teeth and its management. Background: Synodontia refers to the fusion of teeth and is a morphological developmental dental aberration. This anomaly is also acknowledged with different terminologies like fusion, germination, and concrescence. Synodontia with two teeth is not uncommon however it is found sporadic in primary dentition. This type of anomaly can include two or more teeth; if the number is two, it is called as double tooth and if three, is called as triple tooth or triplication defect or triploid tooth. Case Description: In this article, we report an unusual case of triplication of primary teeth occurring unilaterally between the upper deciduous right central, lateral incisor and supernumerary tooth. The triple tooth was extracted under local anesthesia and was sectioned at three levels that are coronal, middle, and cervical one-third, and analyzed by Cone-beam Computerized Tomography (CBCT) respectively. The coronal segment revealed three individual pulp chambers, middle third and apical third revealed one single unified pulp chamber. Conclusion: Triple tooth in a triangular configuration with mixture of incomplete fusion at the coronal portion and cervical third and complete fusion at middle and apical third of root is an elusive anomaly. Clinical significance: This aberrant fusion of two deciduous incisors with supernumerary tooth had been documented as rare anomaly, so its early diagnosis and management protocol is imperative to comprehend. How to cite this article: Ahuja V, Verma J, Bhargava A, et al. "Triple Tooth Synodontia" of Primary Incisors in Triangular Configuration: A Rare Aberrant Case Report. Int J Clin Pediatr Dent 2022;15(6):779-783.

Reconnoitering the Association of Gingival Melanin Pigmentation With Skin Color, Age, and Sex in Pre-School Children of Hazaribag: A Cross-Sectional Study.

Introduction The purpose of this study was to compare and evaluate the association of gingival melanin pigmentation with age, sex, and skin tone in pre-school children of Hazaribag, Jharkhand, India. Materials and methods The study included 310 children aged 3 to 6 years, who were divided according to skin color into the following: group I: Fair, group II: wheatish, group III: brown; and group IV: dark. The children were selected using a census method where all participants fulfilling inclusion criteria were selected from the outpatient department of the Department of Pediatric and Preventive Dentistry, Hazaribag College of Dental Sciences and Hospital, Hazaribag. The subjects were further grouped into different age groups of 3-4 years, 4-5 years, 5-6 years, and 6 years. Subjects were examined in natural daylight, and gingival melanin pigmentation was assessed using the Dummett-Gupta Pigmentation Index. The scoring was done for each arch segment. These categorical data were analyzed using the chi-square test. Results Gingival pigmentation was found to increase with increasing age, whereas female subjects showed less pigmentation than males. Majority of the children had wheatish complexion, and a significant positive correlation was found between skin tone and gingival pigmentation; the darker the skin tone, the darker was the gingival pigmentation. Anatomically, greater pigmentation was found in the anterior than posterior region of arches. Greater pigmentation was found in the labial than lingual region; greater pigmentation was also reported in the mandible than maxilla. Conclusion There was a positive correlation of gingival melanin pigmentation with age, sex, and skin tone in pre-school children. Clinical significance The association of gingival pigmentation with skin color had been documented, but very few studies have been published on adults and children on this subject. However, there is no study that evaluates the association of gingival melanin pigmentation with age, sex, and skin tone of children of pre-school age.

Metal-ion batteries for electric vehicles: current state of the technology, issues and future perspectives.

The batteries based on metals-ions have the potential to meet the future needs of electric vehicle (EV) applications. This article reviews the key technological developments and scientific challenges of a broad range of Li-ion, Mg-ion and Al-ion batteries for electric vehicles. The fundamental configurations and corresponding reaction mechanisms of metal-ion strategies are tangibly discussed in this review article. After a brief revision of the fundamentals, the performance is analysed among Li-ion, Mg-ion and Al-ion battery technologies. The key parameters for the present compilation are the abundance, the volumetric capacity, the gravimetric capacity, the cycling life, cost and safety. Further, it summarizes the recycling methodologies, strengths and limitations of these batteries. Finally, future directions of all these batteries are highlighted and discussed.

COVID-19 infection: Disease detection and mobile technology.

BACKGROUND: A pneumonia outbreak of unknown etiology took place in Wuhan, Hubei province, China and spread quickly worldwide in December 2019. Chinese Center for Disease Control and Prevention identified a novel beta-coronavirus called 2019-nCoV, now officially known as severe acute respiratory syndrome coronavirus 2 (SARS-CoV2) that is responsible for the pandemic. The coronavirus COVID-19 affected 215 countries and territories around the world and more than 99 hundred thousand people at present (Nature Nanotechnology, 2020). At present, there are no specific vaccines or treatments available for COVID-19. However, there are many ongoing clinical trials evaluating potential treatments. At this time the experts recommend precautions such as social distancing, hand washing, and wearing face masks to reduce disease transmission. This review article aims to improve the readers' awareness towards the important role of mobile technology for SARS-CoV-2. METHODOLOGY: To achieve this objective, we performed a COVID-19 literature review from various sources that include data from the published articles as well as World Health Organization reports on coronavirus disease and how mobile technology is useful to fight against this disease. RESULTS: Mobile technology can be helpful in mapping disease spread and provides an easy way to provide awareness that promotes safety and adoption of necessary precautions to mitigate and stop community transmission. CONCLUSION: The spread rate of COVID-19 is very high and until now no vaccines are available to control this disease. To this end we should leverage other avenues such as digital technologies to protect ourselves from this disease. Mobile technology such as smartphones are playing an important role in this pandemic, by launching apps to track coronavirus infected people. These apps are very easy to use and provide self-isolation guidelines as well as other safety tips.

Super protective anti-bacterial coating development with silica-titania nano core-shells.

In the present study, we have developed an anti-bacterial as well as mechanically-strengthened super protective coating material, which can be used as a marine antifouling paint. In this research, silica, titania and silica-titania core-shell nanoparticles were individually prepared via sol-gel and peptization processes. The idea behind the synthesis of core-shell nanoparticles was to utilize the mechanical strength of silica and the antimicrobial property of TiO2 together. These nanoparticles were characterized via dynamic light scattering, UV-Visible spectroscopy, X-ray diffraction, scanning electron microscopy, energy-dispersive X-ray spectroscopy, transmission electron microscopy and X-ray photoelectron spectroscopy. Coating formulations were developed with two types of model binders, i.e., solvent-based polyurethane and water-based poly-acrylic, containing all nanoparticles individually at various concentrations for a better comparative study. These coating formulations were applied onto mild steel for anti-bacterial testing that was performed against Escherichia coli and Bacillus. The nanoparticle concentration was varied from 1% (wt) to 6% (wt). The best anti-bacterial result was obtained with 4% (wt) of silica-titania core-shell nanoparticles prepared via the peptization process among all the nanoparticles. The scratch testing was performed successfully using an Erichsen scratch tester; the formulated PU coating passed up-to 20 N load with good adhesion, impact resistance, flexibility and has shown satisfactory anti-corrosion performance.

Exendin-4 attenuates brain mitochondrial toxicity through PI3K/Akt-dependent pathway in amyloid beta (1-42)-induced cognitive deficit rats.

Alzheimer's disease (AD) is a progressive neurodegenerative disorder characterized by memory loss, disorientation and gradual deterioration of intellectual ability. In the pharmacotherapy of AD, the mitochondrial protective activity of Exendin-4 in experimental studies is yet to be established though its effectiveness is demonstrated in these patients. Therefore, the mitochondria protective activity of Exendin-4 (5 µg/kg, i.p.) was investigated in hippocampus and pre-frontal cortex (PFC) of AD-like animals. The amyloid beta (Aß) was injected through bilateral intracerebroventricular route into lateral ventricles to induce AD-like manifestations in the male rats. Exendin-4 significantly attenuated Aß-induced memory-deficits in the Morris water maze and Y-maze test protocols. Exendin-4 significantly decreased Aß-induced increase in the level of Aß in both brain regions. Exendin-4 significantly increased Aß-induced decrease in acetylcholine level and activity of cholineacetyl transferase in all brain regions. Moreover, Exendin-4 significantly decreased Aß-induced increase in the activity of acetylcholinestrase in both the brain regions. E4 significantly increased Aß-induced decrease in mitochondrial function, integrity, respiratory control rate and ADP/O in all brain regions. Further, Exendin-4 significantly decreased Aß-induced increase in the mitochondrial complex enzyme-I, IV and V activities in all brain regions. Furthermore, Exendin-4 significantly increased Aß-induced decrease in the level of phosphorylated Akt and the ratio of phosphorylated Akt to Akt in both brain regions. However, LY294002 diminished the therapeutic effects of Exendin-4 on behavioral, biochemical and molecular observations in AD-like animals. Pearson's analysis showed that the attributes of mitochondrial dysfunction (MMP and RCR) exhibited significant correlation to the loss in memory formation, level of Aß and cholinergic dysfunction in these animals. Thus, it can be speculated that Exendin-4 may mitigate AD-like manifestations including mitochondrial toxicity perhaps through PI3K/Akt-mediated pathway in the experimental animals. Hence, Exendin-4 could be a potential therapeutic alternative candidate in the management of AD.

Development of coating formulation with silica-titania core-shell nanoparticles against pathogenic fungus.

In the present study, we developed an antifungal coating formulation using silica, titania and silica-titania core-shell nanoparticles individually. The idea behind the synthesis of core-shell nanoparticles was to use the mechanical strength of silica and the antimicrobial property of TiO2 together. These nanoparticles were characterized by dynamic light scattering, transmission electron microscopy, scanning electron microscopy, EDX, FTIR and X-ray diffraction. Silica nanoparticles of 92 nm were prepared by the sol-gel process, while TiO2 nanoparticles and nano-core-shells were prepared through the peptization process with a size of 77 and 144 nm separately. The antifungal effect of the prepared nanoparticles was observed in potato dextrose agar media using the concentration of nanoparticles at 1 wt%. These nanoparticles were incorporated in two types of binder, polyurethane and polyacrylic, with the same concentration of nanoparticles. Coatings were applied on tiles, dried and tested against pathogenic fungus, and fungus growth reduction was observed up to 7-10 days. Coatings developed with TiO2 nanoparticles have shown good growth reduction of pathogenic fungus, but coatings formulated with silica-titania core-shell nanoparticles killed the fungus fusarium completely and have shown around 90% growth reduction for acremonium species also.

SBS vs Inhouse Recycling Methods-An Invitro Evaluation.

INTRODUCTION: In today's world of economic crisis it is not feasible for an orthodontist to replace each and every debonded bracket with a new bracket- quest for an alternative thrives Orthodontist. The concept of recycling bracket for its reuse has evolved over a period of time. Orthodontist can send the brackets to various commercial recycling companies for recycling, but it's impractical as these are complex procedures and require time and usage of a new bracket would seem more feasible. Thereby, in-house methods have been developed. The aim of the study was to determine the SBS (Shear Bond Strength) and to compare, evaluate the efficiency of in house recycling methods with that of the SBS of new brackets. MATERIALS AND METHODS: Five in-house-recycling procedures-Adhesive Grinding Method, Sandblasting Method, Thermal Flaming Method, Buchman method and Acid Bath Method were used in the present study. Initial part of the study included the use of UV/Vis spectrophotometer where in the absorption level of base of new stainless steel bracket is compared with the base of a recycled bracket. The difference seen in the UV absorbance can be attributed to the presence of adhesive remnant. For each recycling procedure the difference in UV absorption is calculated. New stainless steel brackets and recycled brackets were tested for its shear bond strength with Instron testing machine. Comparisons were made between shear bond strength of new brackets with that of recycled brackets. The last part of the study involved correlating the findings of UV/Vis spectrophotometer with the shear bond strength for each recycling procedure. RESULTS: Among the recycled brackets the Sandblasting technique showed the highest shear bond strength (19.789MPa) and the least was shown by the Adhesive Grinding method (13.809MPa). CONCLUSION: The study concludes that sand blasting can be an effective choice among the 5 in house methods of recycling methods.