RESUMO
Using the rapid filtration technique to investigate Ca2+ movements across the sarcoplasmic reticulum (SR) membrane, we compare the initial phases of Ca2+ release and Ca2+ uptake in malignant hyperthermia susceptible (MHS) and normal (N) pig SR vesicles. Ca2+ release is measured from passively loaded SR vesicles. MHS SR vesicles present a 2-fold increase in the initial rate of calcium release induced by 0.3 microM Ca2+ (20.1 +/- 2.1 vs. 6.3 +/- 2.6 nmol mg-1 s-1). Maximal Ca2+ release is obtained with 3 microM Ca2+. At this optimal concentration, rate of Ca2+ efflux in absence of ATP is 55 and 25 nmol mg-1 s-1 for MHS and N SR, respectively. Ca(2+)-induced Ca2+ release is inhibited by Mg2+ in a dose-dependent manner for both MHS and N pig SR vesicles (K1/2 = 0.2 mM). Caffeine (5 mM) and halothane (0.01% v/v) increase the Ca2+ sensitivity of Ca(2+)-induced Ca2+ release. ATP (5 mM) strongly enhances the rate of Ca2+ efflux (to about 20-40-fold in both MHS and N pig SR vesicles). Furthermore, both types of vesicles do not differ in their high-affinity site for ryanodine (Kd = 12 nM and Bmax = 6 pmol/mg), lipid content, ATPase activity and initial rate of Ca2+ uptake (0.948 +/- 0.034 vs. 0.835 +/- 0.130 mumol mg-1 min-1 for MHS and N SR, respectively). Our results show that MH syndrome is associated to a higher rate of Ca2+ release in the earliest phase of the calcium efflux.
Assuntos
Cálcio/metabolismo , Hipertermia Maligna/metabolismo , Retículo Sarcoplasmático/metabolismo , Adenosina Trifosfatases/metabolismo , Animais , Transporte Biológico , Cafeína/farmacologia , Canais de Cálcio/efeitos dos fármacos , Canais de Cálcio/metabolismo , Filtração , Halotano/farmacologia , Cinética , Magnésio/farmacologia , Rianodina/metabolismo , Retículo Sarcoplasmático/efeitos dos fármacos , Retículo Sarcoplasmático/enzimologia , SuínosRESUMO
Skinned fibre experiments were conducted to determine if guanine nucleotide-binding proteins play a role in excitation-contraction coupling of skeletal muscle. By itself, the GTP-gamma S, a non hydrolysable GTP analogue was unable to induce calcium release from the sarcoplasmic reticulum, even at concentrations as high as 500 microM. However, calcium- or caffeine-induced calcium releases were enhanced by GTP-gamma S in micromolar concentrations. This response was blocked by GDP-beta S or Pertussis toxin. 32P-ADP-ribosylation catalysed by Pertussis toxin, radiolabelled G-protein alpha subunits in the range of 40 kDa on membrane subcellular fractions of rat skeletal muscle. Using Western blot analysis with antibodies raised against the bovine transducin, G-proteins were identified in frog and rat skeletal muscle subcellular fractions. In most of the muscle fractions (plasma membrane, T-tubules, triads, sarcoplasmic reticulum), the anti-beta subunit antibodies recognized a 36 kDa protein which comigrated with transducin beta subunit. It appears therefore that some of the G-proteins identified by ADP-ribosylation or immunostaining in several subcellular fractions from skeletal muscle, are implicated in the modulation of calcium release from sarcoplasmic reticulum. These results suggest that a Pertussis toxin sensitive G-protein is present at the loci of E-C coupling, and that it serves to regulate the calcium release.
Assuntos
Cálcio/metabolismo , Proteínas de Ligação ao GTP/fisiologia , Músculos/metabolismo , Retículo Sarcoplasmático/metabolismo , Adenosina Difosfato Ribose/metabolismo , Animais , Cafeína/farmacologia , Bovinos , Guanosina 5'-O-(3-Tiotrifosfato) , Guanosina Difosfato/análogos & derivados , Guanosina Difosfato/farmacologia , Guanosina Trifosfato/análogos & derivados , Guanosina Trifosfato/farmacologia , Immunoblotting , Técnicas In Vitro , Contração Muscular/efeitos dos fármacos , Contração Muscular/fisiologia , Toxina Pertussis , Rana esculenta , Ratos , Frações Subcelulares/metabolismo , Tionucleotídeos/farmacologia , Transducina/análise , Fatores de Virulência de Bordetella/farmacologiaRESUMO
Using whole cell patch clamp recordings on unfertilized eggs of the ascidian Ciona intestinalis, we are able to detect ryanodine receptors within the oocytes. Our approach is based on measurements of the voltage-activated inward calcium currents. Two types of Ca2+ currents have been described on the oocyte membrane of Ciona: a low threshold slowly activating current, and a high threshold faster one. We show here that caffeine induces a decrease in the intensity of the Ca2+ currents, when applied either externally or internally from the mouth of a patch pipette. Caffeine application mimics fertilization which transiently decreases the high threshold Ca2+ current density during density during the first meiotic cycle. Ryanodine (> 1 nM) has an effect similar to caffeine. This partial decrease in Ca2+ current density elicited by caffeine or ryanodine is prevented by intracellular application of the calcium chelator BAPTA, then imputable to calcium release. In summary, the depolarization-induced Ca2+ current intensity allows monitoring of an intracellular calcium store which is sensitive to low concentrations of ryanodine in Ciona oocytes. Further identification of a ryanodine receptor was obtained by immunological staining with antibodies against mammalian skeletal muscle ryanodine receptor. Ryanodine receptors were asymmetrically localized in the cortex of Ciona eggs. We discuss the methodological relevance of our patch-clamp approach, in connection with the possible biological role of such a ryanodine receptor in the early stages of development.
Assuntos
Canais de Cálcio/análise , Cálcio/metabolismo , Proteínas Musculares/análise , Oócitos/fisiologia , Rianodina/farmacologia , Animais , Cafeína/farmacologia , Canais de Cálcio/metabolismo , Quelantes/farmacologia , Ciona intestinalis , Ácido Egtázico/análogos & derivados , Ácido Egtázico/farmacologia , Fertilização , Proteínas Musculares/metabolismo , Oócitos/química , Técnicas de Patch-Clamp , Canal de Liberação de Cálcio do Receptor de RianodinaRESUMO
In striated muscles, excitation-contraction coupling is mediated by the functional interplay between dihydropyridine receptor L-type calcium channels (DHPR) and ryanodine receptor calcium-release channel (RyR). Although significantly different molecular mechanisms are involved in skeletal and cardiac muscles, bidirectional cross-talk between the two channels has been described in both tissues. In the present study using surface plasmon resonance spectroscopy, we demonstrate that both RyR1 and RyR2 can bind to structural elements of the C-terminal cytoplasmic domain of alpha(1C). The interaction is restricted to the CB and IQ motifs involved in the calmodulin-mediated Ca(2+)-dependent inactivation of the DHPR, suggesting functional interactions between the two channels.
Assuntos
Canais de Cálcio Tipo L/metabolismo , Cálcio/metabolismo , Músculo Esquelético/metabolismo , Miocárdio/metabolismo , Canal de Liberação de Cálcio do Receptor de Rianodina/metabolismo , Sequência de Aminoácidos , Animais , Sítios de Ligação , Canais de Cálcio Tipo L/química , Dados de Sequência Molecular , Ligação Proteica , Coelhos , Ressonância de Plasmônio de SuperfícieRESUMO
Calcium release from sarcoplasmic reticulum was investigated using skinned fibers isolated from rabbit semitendinosus and psoas muscles, representative of slow and fast fibers, respectively. In both types of fibers, halothane at the concentration of 0.03% (v/v) enhanced the Ca2(+)-induced calcium release. In the absence of cytoplasmic free Ca2+, halothane induced calcium release in a dose-dependent manner, with a similar sensitivity for both semitendinosus and psoas fibers. These results are discussed in connection with muscular diseases such as malignant hyperthermia in which the crisis is triggered during anesthesia by halothane.
Assuntos
Cálcio/metabolismo , Halotano/farmacologia , Músculos/metabolismo , Retículo Sarcoplasmático/metabolismo , Trifosfato de Adenosina/farmacologia , Animais , Cálcio/farmacologia , Cinética , Músculos/efeitos dos fármacos , Coelhos , Retículo Sarcoplasmático/efeitos dos fármacosRESUMO
The striated remotor muscle of the lobster antenna has an extraordinarily profuse sarcoplasmic reticulum as shown by electron microscopy. Gel electrophoresis reveals a simple protein composition in which the Ca2+-ATPase predominates. Vesicles of sarcoplasmic reticulum (SR) from this remotor are shown to operate Ca2+ binding, Ca2+ transport, and Ca2+-activated hydrolysis of ATP with an usual efficiency (2 Ca2+ transported per ATP hydrolysed, 4 mumol ATP hydrolysed/mg protein/min). Skinned fiber experiments were performed. They indicate behaviour of the remotor expected from observations by EM and gel electrophoresis: contraction of low maximal intensity under Ca2+ excitation, long internal diffusion time due to the large volume of SR to be crossed, and large Ca2+ content released in a caffeine-sensitive manner.
Assuntos
ATPases Transportadoras de Cálcio/metabolismo , Músculos/ultraestrutura , Retículo Sarcoplasmático/ultraestrutura , Animais , Cinética , Microscopia Eletrônica , Músculos/enzimologia , Nephropidae , Retículo Sarcoplasmático/enzimologiaRESUMO
The different membrane systems and proteins involved in the control of intracellular calcium movements in the skeletal muscle cell are described. These include the sarcoplasmic reticulum, that Ca(++)-ATPase sarcoplasmic reticular calcium pump, transverse tubules, calcium channels, and the ryanodine receptor protein. The significance of these systems is shown clearly in the myopathies, where the main errors involved do not concern the contractile system, but the command and control mechanisms.
Assuntos
Canais de Cálcio/metabolismo , Cálcio/metabolismo , Contração Muscular , Músculos/citologia , ATPases Transportadoras de Cálcio/metabolismo , Di-Hidropiridinas , Humanos , Músculos/metabolismo , Doenças Musculares/fisiopatologia , Miofibrilas/metabolismo , Receptores de Superfície Celular/isolamento & purificação , Rianodina , Retículo Sarcoplasmático/metabolismoAssuntos
Cloroplastos , Pigmentos Biológicos , Proteínas de Plantas , Plantas , Ar , Clorofila , Magnetismo , Matemática , Conformação Molecular , Rotação Ocular , Espectrofotometria , Análise Espectral , UltrassomAssuntos
Pigmentos da Retina , Rodopsina , Cristalografia , Detergentes , Deutério , Micelas , Peso Molecular , NêutronsAssuntos
Células Fotorreceptoras/ultraestrutura , Segmento Externo da Célula Bastonete/ultraestrutura , Animais , Bovinos , Escuridão , Luz , Ranidae , Rodopsina/análise , Espectrofotometria/instrumentação , Espectrofotometria/métodos , Espectrofotometria Infravermelho/métodos , Espectrofotometria Ultravioleta/métodosRESUMO
Before synaptogenesis, early excitability implicating voltage-dependent and transmitter-activated channels is known to be crucial for neuronal development. We previously showed that preplate (PP) neurons of the mouse neocortex express functional Na(+) channels as early as embryonic day 12. In this study, we investigated the role of these Na(+) channels in signaling during early development. In the neocortex of embryonic-day-13 mice, activation of Na(+) channels with veratridine induced a large Ca(2+) response throughout the neocortex, even in cell populations that lack the Na(+) channel. This Na(+)-dependent Ca(2+) activity requires external Ca(2+) and is completely blocked by inhibitors of Na(+)/Ca(2+) exchangers. Moreover, veratridine-induced Ca(2+) increase coincides with a burst of exocytosis in the PP. In parallel, we show that Na(+) channel stimulation enhances glutamate secretion in the neocortical wall. Released glutamate triggers further Ca(2+) response in PP and ventricular zone, as indicated by the decreased response to veratridine in the presence of alpha-amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid receptor and NMDA-receptor inhibitors. Therefore, the combined activation of the Na(+) channel and the Na(+)/Ca(2+) exchanger triggers Ca(2+) signaling in the PP neurons, leading to glutamate secretion, which amplifies the signal and serves as an autocrine/paracrine transmitter before functional synapses are formed in the neocortex. Membrane depolarization induced by glycine receptors activation could be one physiological activator of this Na(+) channel-dependent pathway.
Assuntos
Cálcio/química , Ácido Glutâmico/metabolismo , Canais de Sódio/química , Sódio/química , Animais , Ácido Aspártico/química , Encéfalo/metabolismo , Cálcio/metabolismo , Exocitose , Glicina/química , Camundongos , Camundongos Endogâmicos C57BL , Modelos Biológicos , N-Metilaspartato/química , Neocórtex/metabolismo , Neocórtex/patologia , Neurônios/metabolismo , Receptores de AMPA/metabolismo , Receptores de Glicina/metabolismo , Receptores de N-Metil-D-Aspartato/química , Sódio/metabolismo , Software , Taurina/química , Fatores de Tempo , Veratridina/farmacologia , Ácido gama-Aminobutírico/metabolismoRESUMO
Two voltage-dependent calcium currents have been described in unfertilized eggs of the ascidian Ciona intestinalis: a low threshold, slowly activating current, and a high threshold fast one. According to the classical criteria for classification of calcium currents, they both share some of the features of L-like and T-like currents. We have studied these two calcium currents further by measuring their sensitivity to permeant ions, temperature and inhibitors. Both currents were sensitive to relatively high concentrations of nitrendipine, which was a selective blocker of the low threshold channel. The lanthanide ion gadolinium was a potent blocker of the low threshold current, and cadmium preferentially inhibited the high threshold current. The two calcium currents were regulated in a different manner after fertilization. The density of the high threshold current remained relatively constant, while the low threshold current was lost by the time of first cleavage. This loss following fertilization is similar to the loss of a low threshold sodium current in fertilized eggs of the ascidian Boltenia villosa. Block of the cell cycle with various compounds did not prevent loss of the low threshold calcium current. This observation adds weight to the hypothesis that a loss of excitability is a general property of early development. We conclude that fertilization can differentially modulate channel populations before first cleavage. The mechanism by which this occurs in the ascidian embryo has yet to be discovered.
Assuntos
Canais de Cálcio/fisiologia , Ciona intestinalis/embriologia , Embrião não Mamífero/fisiologia , Animais , Cádmio/farmacologia , Ciona intestinalis/fisiologia , Feminino , Gadolínio/farmacologia , Nitrendipino/farmacologia , Oócitos/fisiologia , Canais de Sódio/fisiologiaRESUMO
In ascidian oocytes, numerous calcium signaling events occur at fertilization which contribute to resume and complete meiosis, and determine the three embryonic axes. The main ooplasmic and intracellular calcium channels at work in the calcium signaling of the one-cell embryo have different roles and fates when the first mitosis begins. By whole-cell patch-clamp recording, we observed different families of these calcium channels in the blastomeres of Phallusia mammillata two-cell ascidian embryos. Membrane capacitance has been measured to evaluate the oocyte and blastomere surface area, allowing certification of the exact time of cell division. At the two-cell stage, no difference was observed in the density of voltage-dependent calcium channels in each blastomere, or in the ryanodine-sensitive calcium stores. In contrast, a bilateral asymmetry was recorded for the ooplasmic channels responsible for calcium entry after calcium store depletion: they could be activated only in the blastomere not wearing the polar bodies. The same laterality was observed in the InsP3-induced internal calcium release. Moreover, this asymmetry included a one-way communication in the InsP3-dependent calcium signaling between the two blastomeres. These results enhance the understanding of the early steps of development, and underscore the interest for ascidians in studies of polarity patterning.
Assuntos
Blastômeros/fisiologia , Sinalização do Cálcio/fisiologia , Inositol 1,4,5-Trifosfato/metabolismo , Animais , Canais de Cálcio/metabolismo , Canais de Cálcio/fisiologia , Fase de Clivagem do Zigoto/fisiologia , Urocordados/embriologiaRESUMO
Variations in capacitance or cell surface area were recorded on patch-clamped eggs of the ascidian Ciona intestinalis between the resumption of meiosis and the first mitotic cleavages. The membrane surface area increased within the first minutes after fertilization and then oscillated in phase with the cell cycles of the two meiotic divisions and first mitotic cleavage. With drugs, we generated two opposite situations (removal and insertion) or artificial variation in capacitance. In unfertilized eggs, cytochalasin induced a drop in capacitance linked to a decrease in calcium current intensity and specifically disturbed membrane removal linked to the first meiotic division cycle. It left unaffected the following cycles, in agreement with previous results that only the first meiosis cycle is microfilament dependent. In fertilized eggs, membrane removal at each cycle was hindered by emetine, an inhibitor of protein synthesis. The resulting membrane extrusion was observed in sections by electron microscopy and was linked to an increase in calcium current intensity. These fluctuations in surface area never involved the microtubule network, since nocodazole had no effect on any cycle. The fluctuations of membrane surface area after meiosis resumption in phase with cell cycles in Ciona oocytes paralleled the pattern previously described in the ascidian Boltenia villosa. This may reflect the mechanism by which the oocyte regulates, with possibly different mediators at each cycle, the connection between cell surface and internal membrane networks. This interrelation includes the insertion and removal of ion channels necessary to developmental control.
Assuntos
Ciclo Celular/fisiologia , Oócitos/citologia , Animais , Canais de Cálcio/efeitos dos fármacos , Canais de Cálcio/fisiologia , Ciclo Celular/efeitos dos fármacos , Membrana Celular/efeitos dos fármacos , Membrana Celular/ultraestrutura , Ciona intestinalis , Citocalasina B/farmacologia , Emetina/farmacologia , Feminino , Fertilização , Técnicas In Vitro , Meiose , Potenciais da Membrana/efeitos dos fármacos , Microscopia Eletrônica , Mitose , Oócitos/efeitos dos fármacos , Oócitos/fisiologia , Fatores de TempoRESUMO
The conductance change after fertilization in the oocyte of the ascidian Ciona intestinalis has been followed by the whole cell patch-clamp technique. Two new inward currents, which are absent in unfertilized eggs, are elicited by hyperpolarization from a holding potential of +20 mV, which is the resting potential soon after fertilization. These currents reach their maximum level during the first meiotic division cycle, and then decrease in intensity, becoming almost undetectable at the 2-cell stage. These currents are most easily seen at high concentrations of barium. At least one, and likely both, of these currents appears to be carried by Ca ions. One of the currents is blocked by low concentrations of gadolinium; the other one is blocked by higher concentrations, although gadolinium at these levels does not block fertilization and the associated early depolarizing jump of the eggs. Thus these currents are not carried by channels that mediate the fertilization current. However, gadolinium blocks normal transition to 2-cell stage and blocks current oscillations synchronous to free calcium oscillations that occur normally in eggs around meiosis II. The electrical signature of calcium-release activated currents, taken together with these findings, suggests that these inward currents ensure a calcium entry pathway throughout meiosis. A plausible function of these currents may be to refill the Ca stores that are depleted after fertilization and that are required to progress into mitotic cell division. This interpretation is reinforced by experiments on unfertilized eggs with intracellular Ca stores depleted by thapsigargin, where both the newly described currents are observed.
Assuntos
Cálcio/metabolismo , Fertilização , Meiose , Óvulo/metabolismo , Urocordados/embriologia , Animais , ATPases Transportadoras de Cálcio/antagonistas & inibidores , Embrião não Mamífero , Inibidores Enzimáticos/farmacologia , Gadolínio/farmacologia , Transporte de Íons , Cinética , Potenciais da Membrana , Óvulo/efeitos dos fármacos , Óvulo/fisiologia , Terpenos/farmacologia , TapsigarginaRESUMO
Fertilization in the ascidians triggers an activation wave of calcium release followed by intracellular calcium oscillations synchronous with periodic membrane potential excursions during the completion of the meiotic cell cycle. Fertilization also causes a fast decrease in the egg plasma membrane depolarization-activated calcium current and a large increase in capacitance thought to represent membrane addition to the egg surface. We have analyzed the temporal and causal relationships between these changes in the eggs of Phallusia mammillata using whole-cell patch-clamp recording while simultaneously imaging calcium with fura-2 dextran. We have defined the role of ryanodine receptor (RyR) and InsP3 receptor (InsP3R) during fertilization and meiosis by looking at the effects of InsP3, cyclic ADP ribose (cADPR), and ryanodine in perfused oocytes. We show that InsP3 (10 microM perfused through the patch pipette) is able to trigger sustained oscillations in intracellular calcium concentration in unfertilized oocytes, resembling those recorded in fertilized egg completing meiosis. In addition the sustained oscillations resulting from InsP3 perfusion in unfertilized oocytes are sufficient to cause the emission of both polar bodies. In contrast, ryanodine or cADPR never trigger detectable calcium signal in perfused oocytes. Instead, nanomolar concentrations of ryanodine or cADPR cause a capacitance change, implying a net insertion of membrane to the oocyte surface, and trigger a fast decrease in the depolarization-activated calcium current. Both changes are similar to the changes in conductance and capacitance naturally observed following fertilization. These effects, although not associated with measurable calcium signals, are abolished by coperfusion of the calcium chelator BAPTA. In contrast to ryanodine or cADPR, sustained perfusion of the oocyte with nanomolar concentrations of InsP3 causes no capacitance change and a slow and moderate decrease in calcium current. Our observations on inseminated patch-clamped eggs further indicate that membrane insertion, which starts 15-20 sec after the onset of the membrane conductance change at fertilization, can be altered by interfering with the RyR. Our results imply that, in ascidians, as in some mammals, RyR and InsP3R play distinct roles during fertilization.
Assuntos
Canais de Cálcio/fisiologia , Proteínas de Ligação a Calmodulina/metabolismo , Fertilização/fisiologia , Inositol 1,4,5-Trifosfato/metabolismo , Proteínas Musculares/fisiologia , Receptores Citoplasmáticos e Nucleares/fisiologia , Animais , Cálcio/metabolismo , Canais de Cálcio/metabolismo , Eletrofisiologia , Feminino , Receptores de Inositol 1,4,5-Trifosfato , Masculino , Meiose , Proteínas Musculares/metabolismo , Oócitos/fisiologia , Técnicas de Patch-Clamp , Receptores Citoplasmáticos e Nucleares/metabolismo , Canal de Liberação de Cálcio do Receptor de Rianodina , Transdução de Sinais , Interações Espermatozoide-Óvulo/fisiologia , UrocordadosRESUMO
Fertilization of oocytes incites numerous changes relying on Ca(2+) signaling. In inseminated ascidian eggs, an increase in the egg surface membrane, monitored by a change in electrical capacitance, is recorded at the onset of meiosis resumption. This membrane addition to the cell surface is controlled by calcium release through a ryanodine receptor (RyR), sensitive to cyclic ADP-ribose. Using confocal microscopy analysis of ascidian oocytes immunostained with anti-RyR antibody, we show here that this calcium channel is asymmetrically located in the vegetal cortical zone. Interestingly, the increase in cell capacitance occurring at fertilization is correlated with a fluorescent signal, imaged by the marker of vesicle trafficking FM 1-43, located close to the RyR region. Two putative partners of RyR, namely an FKBP-like protein and a calmodulin, are identified in these oocyte extracts by detection of enzyme activity and PCR amplification. Both are necessary to sustain ryanodine receptor activity in these oocytes since the membrane insertion triggered by fertilization is inhibited by the FKBP ligand rapamycin and by a calmodulin antagonist peptide. These findings suggest that exocytosis in ascidian eggs is triggered at fertilization by a functional Ca(2+) release unit operating as a complex of several proteins, including a calmodulin and an immunophilin, around the intracellular calcium channel itself.
Assuntos
Calmodulina/fisiologia , Fertilização , Imunofilinas/fisiologia , Oócitos/fisiologia , Canal de Liberação de Cálcio do Receptor de Rianodina/fisiologia , Urocordados/fisiologia , Sequência de Aminoácidos , Animais , Cálcio/fisiologia , Exocitose , Feminino , Dados de Sequência Molecular , Técnicas de Patch-ClampRESUMO
Different patterns of voltage-dependent ion currents are present in mature eggs and in early embryos of the ascidian Boltenia villosa, as if each ion current is regulated in a different manner between fertilization and the early cleavages of embryogenesis. The ion currents appear and/or disappear with precise timing suggesting that they play important roles at specific times during early development. We investigated changes in three voltage-dependent ion currents (an inwardly rectifying chloride current, a calcium current, and a sodium current) and membrane surface area over time between the resumption of meiosis (with fertilization or activation) and the first mitotic cleavage. Using time-lapse video recordings made during whole-cell patch-clamp experiments, we were able to correlate electrophysiological changes with morphological changes and cell cycle related events. Between fertilization and first cleavage, INa was lost exponentially, the density of ICa remained relatively constant, and the amplitudes of both ICl and membrane surface area fluctuated in time with the cell cycle. ICl and surface area increased whenever the cell began dividing--with the polar body extrusions and the formation of the first cleavage furrow. This suggested that the values of ICl and surface area were largest during interphase and smallest during M-phase of each cell cycle. This hypothesis was supported by an experiment in which entry into M-phase was blocked in fertilized eggs by inhibiting protein synthesis. This prevented the decreases of ICl and surface area but allowed the increases to occur normally. Patterns of change in ion currents are current specific and, as is the case with ICl, are tightly correlated with developmental events.
Assuntos
Urocordados/fisiologia , Animais , Cálcio/metabolismo , Cloretos/metabolismo , Eletrofisiologia , Feminino , Masculino , Meiose/fisiologia , Mitose/fisiologia , Sódio/metabolismo , Interações Espermatozoide-Óvulo/fisiologiaRESUMO
A new, nonenzymatically treated preparation of amphibian sarcolemmal blebs has been used to study the regulation of skeletal muscle ATP-sensitive K+ [K(ATP)] channels. When a frog skeletal muscle fiber is split in half in a Ca(2+)-free relaxing solution, large hemispherical membrane blebs appear spontaneously within minutes without need for Ca(2+)-induced contraction or enzymatic treatment. These blebs readily formed gigaseals with patch pipettes, and excised inside-out patches were found to contain a variety of K+ channels. Most prominent were K(ATP) channels similar to those found in the surface membrane of other muscle and nonmuscle cells. These channels were highly selective for K+, had a conductance of approximately 53 pS in 140 mM K+, and were blocked by internal ATP. The presence of these channels in most patches implies that split-fiber blebs are made up, at least in large part, of sarcolemmal membrane. In this preparation, K(ATP) channels could be rapidly and reversibly blocked by glibenclamide (0.1-10 microM) in a dose-dependent manner. These channels were sensitive to ATP in the micromolar range in the absence of Mg. This sensitivity was noticeably reduced in the presence of millimolar Mg, most likely because of the ability of Mg2+ ions to bind ATP. Our data therefore suggest that free ATP is a much more potent inhibitor of these channels than MgATP. Channel sensitivity to ATP was significantly reduced by ADP in a manner consistent with a competition between ADP, a weak inhibitor, and ATP, a strong inhibitor, for the same inhibitory binding sites. These observations suggest that the mechanisms of nucleotide regulation of skeletal muscle and pancreatic K(ATP) channels are more analogous than previously thought.
Assuntos
Difosfato de Adenosina/farmacologia , Trifosfato de Adenosina/farmacologia , Magnésio/farmacologia , Músculos/ultraestrutura , Canais de Potássio/fisiologia , Sarcolema/ultraestrutura , Trifosfato de Adenosina/antagonistas & inibidores , Animais , Relação Dose-Resposta a Droga , Glibureto/farmacologia , Potenciais da Membrana/efeitos dos fármacos , Potenciais da Membrana/fisiologia , Músculos/efeitos dos fármacos , Músculos/fisiologia , Canais de Potássio/efeitos dos fármacos , Canais de Potássio/ultraestrutura , Rana esculenta , Sarcolema/efeitos dos fármacos , Sarcolema/fisiologiaRESUMO
ADP-ribosyl cyclase catalyzes the synthesis of two structurally and functionally different Ca2+ releasing molecules, cyclic ADP-ribose (cADPR) from beta-NAD and nicotinic acid-adenine dinucleotide phosphate (NAADP) from beta-NADP. Their Ca2+-mobilizing effects in ascidian oocytes were characterized in connection with that induced by inositol 1,4,5-trisphosphate (InsP3). Fertilization of the oocyte is accompanied by a decrease in the oocyte Ca2+ current and an increase in membrane capacitance due to the addition of membrane to the cell surface. Both of these electrical changes could be induced by perfusion, through a patch pipette, of nanomolar concentrations of cADPR or its precursor, beta-NAD, into unfertilized oocytes. The changes induced by beta-NAD showed a distinctive delay consistent with its enzymatic conversion to cADPR. The cADPR-induced changes were inhibited by preloading the oocytes with a Ca2+ chelator, indicating the effects were due to Ca2+ release induced by cADPR. Consistently, ryanodine (at high concentration) or 8-amino-cADPR, a specific antagonist of cADPR, but not heparin, inhibited the cADPR-induced changes. Both inhibitors likewise blocked the membrane insertion that normally occurred at fertilization consistent with it being mediated by a ryanodine receptor. The effects of NAADP were different from those of cADPR. Although NAADP induced a similar decrease in the Ca2+ current, no membrane insertion occurred. Moreover, pretreatment of the oocytes with NAADP inhibited the post-fertilization Ca2+ oscillation while cADPR did not. A similar Ca2+ oscillation could be artificially induced by perfusing into the oocytes a high concentration of InsP3 and NAADP could likewise inhibit such an InsP3-induced oscillation. This work shows that three independent Ca2+ signaling pathways are present in the oocytes and that each is involved in mediating distinct changes associated with fertilization. The results are consistent with a hierarchical organization of Ca2+ stores in the oocyte.