Rutting vocal display in male impala (Aepyceros melampus) and overlap with alarm context.

BACKGROUND: The rutting vocal display of male impala Aepyceros melampus is unique for its complexity among ruminants. This study investigates bouts of rutting calls produced towards potential mates and rival males by free-ranging male impala in Namibia. In particular, a comparison of male rutting and alarm snorts is conducted, inspired by earlier findings of mate guarding by using alarm snorts in male topi Damaliscus lunatus. RESULTS: Rutting male impala produced 4-38 (13.5 ± 6.5) rutting calls per bout. We analyzed 201 bouts, containing in total 2709 rutting calls of five types: continuous roars produced within a single exhalation-inhalation cycle; interrupted roars including few exhalation-inhalation cycles; pant-roars distinctive by a pant-phase with rapidly alternating inhalations and exhalations; usual snorts lacking any roar part; and roar-snorts starting with a short roar part. Bouts mostly started and ended with usual snorts. Continuous roars were the shortest roars. The average duration of the exhalatory phase was longest in the continuous roars and shortest in the pant-roars. The average fundamental frequency (49.7-51.4 Hz) did not differ between roar types. Vocal tract length, calculated by using measurements of the first four vocal tract resonances (formants), ranged within 381-382 mm in all roar types. In the studied male impala, rutting snorts within bouts of rutting calls were longer and had higher values of the upper quartile in the call spectra than alarm snorts produced towards potential danger. CONCLUSIONS: Additional inhalations during the emission of the interrupted and pant-roars prolong their duration compared to the continuous roars but do not affect the fundamental frequency or the degree of larynx retraction while roaring. Alarm snorts are separated from one another by large intervals, whereas the intervals between rutting snorts within bouts are short. Sometimes, rutting snorts alternate with roars, whereas alarm snorts do not. Therefore, it is not the acoustic structure of individual snorts but the temporal sequence and the occasional association with another call type that defines snorts as either rutting or alarm snorts. The rutting snorts of male impala may function to attract the attention of receptive females and delay their departure from a male's harem or territory.

Vocal phenotype of male rutting roars and genetic markers delineate East European red deer (Cervus elaphus) from Central and West European populations.

This study investigates a population of red deer Cervus elaphus, founded by 10 individuals introduced in the nineteenth century from Germany to the Voronezh region of the European part of Southern Russia and then developed without further introductions. We characterize for the first time the vocal phenotype of the Voronezh red deer male rutting calls in comparison with similar data on the Pannonian (native Central European) and Iberian (native West European) red deer obtained by the authors during preceding studies. In addition, we provide for the first time the genetic data on Pannonian red deer. In Voronezh stags, the number of roars per bout (2.85 ± 1.79) was lower than in Pannonian (3.18 ± 2.17) but higher than in Iberian (2.11 ± 1.71) stags. In Voronezh stags, the duration of main (the longest within bouts) roars was longer (2.46 ± 1.14 s) than in Pannonian (1.13 ± 0.50 s) or Iberian (1.90 ± 0.50 s) stags. The maximum fundamental frequency of main roars was similar between Voronezh (175 ± 60 Hz) and Pannonian (168 ± 61 Hz) but higher in Iberian stags (223 ± 35 Hz). Mitochondrial cytochrome b gene analysis of red deer from the three study populations partially supports the bioacoustical data, of closer similarity between Voronezh and Pannonian populations. In contrast, microsatellite DNA analysis delineates Voronezh red deer from either Pannonian or Iberian red deer. We discuss that population bottlenecking might affect the acoustics of the rutting roars, in addition to genotype.

Savannah roars: The vocal anatomy and the impressive rutting calls of male impala (Aepyceros melampus) - highlighting the acoustic correlates of a mobile larynx.

A retractable larynx and adaptations of the vocal folds in the males of several polygynous ruminants serve for the production of rutting calls that acoustically announce larger than actual body size to both rival males and potential female mates. Here, such features of the vocal tract and of the sound source are documented in another species. We investigated the vocal anatomy and laryngeal mobility including its acoustical effects during the rutting vocal display of free-ranging male impala (Aepyceros melampus melampus) in Namibia. Male impala produced bouts of rutting calls (consisting of oral roars and interspersed explosive nasal snorts) in a low-stretch posture while guarding a rutting territory or harem. For the duration of the roars, male impala retracted the larynx from its high resting position to a low mid-neck position involving an extensible pharynx and a resilient connection between the hyoid apparatus and the larynx. Maximal larynx retraction was 108 mm based on estimates in video single frames. This was in good concordance with 91-mm vocal tract elongation calculated on the basis of differences in formant dispersion between roar portions produced with the larynx still ascended and those produced with maximally retracted larynx. Judged by their morphological traits, the larynx-retracting muscles of male impala are homologous to those of other larynx-retracting ruminants. In contrast, the large and massive vocal keels are evolutionary novelties arising by fusion and linear arrangement of the arytenoid cartilage and the canonical vocal fold. These bulky and histologically complex vocal keels produced a low fundamental frequency of 50 Hz. Impala is another ruminant species in which the males are capable of larynx retraction. In addition, male impala vocal folds are spectacularly specialized compared with domestic bovids, allowing the production of impressive, low-frequency roaring vocalizations as a significant part of their rutting behaviour. Our study expands knowledge on the evolutionary variation of vocal fold morphology in mammals, suggesting that the structure of the mammalian sound source is not always human-like and should be considered in acoustic analysis and modelling.

Altai pika (Ochotona alpina) alarm calls: individual acoustic variation and the phenomenon of call-synchronous ear folding behavior.

Non-hibernating pikas collect winter food reserves and store them in hay piles. Individualization of alarm calls might allow discrimination between colony members and conspecifics trying to steal food items from a colony pile. We investigated vocal posture, vocal tract length, and individual acoustic variation of alarm calls, emitted by wild-living Altai pikas Ochotona alpina toward a researcher. Recording started when a pika started calling and lasted as long as possible. The alarm call series of 442 individual callers from different colonies consisted of discrete short (0.073-0.157 s), high-frequency (7.31-15.46 kHz), and frequency-modulated calls separated by irregular intervals. Analysis of 442 discrete calls, the second of each series, revealed that 44.34% calls lacked nonlinear phenomena, in 7.02% nonlinear phenomena covered less than half of call duration, and in 48.64% nonlinear phenomena covered more than half of call duration. Peak frequencies varied among individuals but always fitted one of three maxima corresponding to the vocal tract resonance frequencies (formants) calculated for an estimated 45-mm oral vocal tract. Discriminant analysis using variables of 8 calls per series of 36 different callers, each from a different colony, correctly assigned over 90% of the calls to individuals. Consequently, Altai pika alarm calls are individualistic and nonlinear phenomena might further increase this acoustic individualization. Additionally, video analysis revealed a call-synchronous, very fast (0.13-0.23 s) folding, depression, and subsequent re-expansion of the pinna confirming an earlier report of this behavior that apparently contributes to protecting the hearing apparatus from damage by the self-generated high-intensity alarm calls.

Unusually high-pitched neonate distress calls of the open-habitat Mongolian gazelle (Procapra gutturosa) and their anatomical and hormonal predictors.

In neonate ruminants, the acoustic structure of vocalizations may depend on sex, vocal anatomy, hormonal profiles and body mass and on environmental factors. In neonate wild-living Mongolian gazelles Procapra gutturosa, hand-captured during biomedical monitoring in the Daurian steppes at the Russian-Mongolian border, we spectrographically analysed distress calls and measured body mass of 22 individuals (6 males, 16 females). For 20 (5 male, 15 female) of these individuals, serum testosterone levels were also analysed. In addition, we measured relevant dimensions of the vocal apparatus (larynx, vocal folds, vocal tract) in one stillborn male Mongolian gazelle specimen. Neonate distress calls of either sex were high in maximum fundamental frequency (800-900 Hz), but the beginning and minimum fundamental frequencies were significantly lower in males than in females. Body mass was larger in males than in females. The levels of serum testosterone were marginally higher in males. No correlations were found between either body mass or serum testosterone values and any acoustic variable for males and females analysed together or separately. We discuss that the high-frequency calls of neonate Mongolian gazelles are more typical for closed-habitat neonate ruminants, whereas other open-habitat neonate ruminants (goitred gazelle Gazella subgutturosa, saiga antelope Saiga tatarica and reindeer Rangifer tarandus) produce low-frequency (<200 Hz) distress calls. Proximate cause for the high fundamental frequency of distress calls of neonate Mongolian gazelles is their very short, atypical vocal folds (4 mm) compared to the 7-mm vocal folds of neonate goitred gazelles, producing distress calls as low as 120 Hz.

Remarkable vocal identity in wild-living mother and neonate saiga antelopes: a specialization for breeding in huge aggregations?

Saiga antelopes Saiga tatarica tatarica give birth in large aggregations, and offspring follow the herd soon after birth. Herding is advantageous as anti-predator strategy; however, communication between mothers and neonates is strongly complicated in large aggregations. Individual series of nasal and oral contact calls of mother and neonate saiga antelopes were selected from recordings made with automated recording systems placed near the hiding neonates on the saiga breeding grounds in Northern Kazakhstan during synchronized parturitions of 30,000 calving females. We used for comparison of the acoustic structure of nasal and oral contact calls 168 nasal calls of 18 mothers, 192 oral calls of 21 mothers, 78 nasal calls of 16 neonates, and 197 oral calls of 22 neonates. In the oral calls of either mothers or neonates, formant frequencies were higher and the duration was longer than in the nasal calls, whereas fundamental frequencies did not differ between oral and nasal calls. Discriminant function analysis (DFA) based on six acoustic variables, accurately classified individual identity for 99.4% of oral calls of 18 mothers, for 89.3% of nasal calls of 18 mothers, and for 94.4% of oral calls of 18 neonates. The average value of correct classification to individual was higher in mother oral than in mother nasal calls and in mother oral calls than in neonate oral calls; no significant difference was observed between mother nasal and neonate oral calls. Variables mainly responsible for vocal identity were the fundamental frequency and the second and third formants in either mothers or neonates, and in either nasal or oral calls. The high vocal identity of mothers and neonates suggests a powerful potential for the mutual mother-offspring recognition in dense aggregations of saiga antelopes as an important component of their survival strategy.

The power of oral and nasal calls to discriminate individual mothers and offspring in red deer, Cervus elaphus.

BACKGROUND: In most species, acoustical cues are crucial for mother-offspring recognition. Studies of a few species of ungulates showed that potential for individual recognition may differ between nasal and oral contact calls. RESULTS: Vocalizations of 28 hinds and 31 calves of farmed Iberian red deer (Cervus elaphus hispanicus) were examined with discriminant function analyses (DFA) to determine whether acoustic structure of their oral and nasal contact calls encodes information about the caller's identity. Contact calls were elicited by brief separation of individually identified animals by a distance over 10 m or by a bar fence. Both oral and nasal calls of both hinds and calves showed high potential to discriminate individuals. In hinds, individuality was significantly higher in the oral than in the nasal calls, whereas in calves, individuality was equally well expressed in both oral and nasal calls. For calves, the maximum fundamental frequency was higher and the duration was longer in oral calls than in nasal calls. For hinds, the maximum fundamental frequency and the duration were indistinguishable between oral and nasal calls. Compared to the pooled sample of oral and nasal calls, separate oral or nasal call samples provided better classifying accuracy to individual in either hinds or calves. Nevertheless, in both hinds and calves, even in the pooled sample of oral and nasal calls, the degree of individual identity was 2-3 times greater than expected by chance. For hinds that provided calls in both years, cross-validation of calls collected in 2012 with discriminant functions created with calls from 2011 showed a strong decrease of classifying accuracy to individual. CONCLUSIONS: These results suggest different potentials of nasal and oral calls to allow the discrimination of individuals among hinds, but not among red deer calves. The high potential of individual recognition even with the pooled sample of oral and nasal calls allows mother and young to remember only one set of acoustic variables for mutual vocal recognition. Poor between-year stability of individual characteristics of hind oral and nasal calls would require updating keys to individual recognition each calving season.

Vocal group signatures in the goitred gazelle Gazella subgutturosa.

The potential for vocal modification in mammals has recently been of great interest. This study focuses on the potential for vocal matching in juvenile and adolescent goitred gazelles Gazella subgutturosa that were group housed as part of an animal management programme. Two groups of animals (16 and 19 unrelated individuals, respectively) were recorded at two different ages, juvenile and adolescent, regarding 20-25 calls per individual per age; each group was evaluated in a separate year. Vocal similarity of group members compared to non-members was prominent in both ages, but higher in juveniles. Individual identity was prominent in both ages and higher in adolescents. The more prominent vocal indicators of group membership in juveniles could be related to their higher social dependence compared to adolescents. The more individualized calls of adolescents could be a mechanistic consequence of more stable growth at older age. Our results suggest vocal plasticity of goitred gazelles under social influences. These data add to recent evidence about domestic goat kids Capra hircus, suggesting that vocalizations of species that are not capable of imitation are more flexible than previously thought.

Relationship between acoustic traits of protesting cries of domestic kittens (Felis catus) and their individual chances for survival.

Domestic cat (Felis catus) mothers may rely on offspring cries to allocate resources in use of individuals with greater chances for survival and sacrifice the weak ones in case of impossibility to raise the entire large litter. Potential victims of this maternal strategy can enhance their chances of survival, by producing vocalizations with traits mimicking those of higher-quality offspring. We compared acoustic traits of 4990 cries produced during blood sampling by 57 two-week-old captive feral kittens (28 males, 29 females); 47 of them survived to 90 days of age and 10 died by reasons not related to traumas or aggression. No relationship was found between acoustic parameters and kitten survival, however, positive relationship was found between survival and body weight. The cries had moderate cues to individuality and lacked cues to sex. Body weight correlated positively with fundamental frequency and negatively with call rate, duration, peak frequency and power quartiles. We discuss that dishonesty of acoustic traits of kitten quality could develop as adaptation for misleading a mother from allocation resources between the weaker and stronger individuals, thus enhancing individual chances for survival for the weaker littermates. Physical constraint, as body weight, may prevent extensive developing the deceptive vocal traits.

Comparison of ultrasonic isolation calls of pure-breeding and interspecies hybrid Phodopus dwarf hamster pups.

In mammalian cross-species hybrids, parameters of voice calls, produced by vocal fold vibrations, are intermediate between parental species. Inheritance of ultrasonic calls, produced by whistle mechanism, is unstudied for hybrids. We examined 4000 pup ultrasonic isolation-induced calls for peak power of call fundamental frequency and for call duration in 4-8-day-old captive hamsters of four Study Groups: pure Phodopus sungorus; pure P. campbelli of two populations (Mongolian and Kosh-Agach) and hybrids between male P. sungorus and female P. campbelli (Kosh-Agach). All Study Groups produced two categories of ultrasonic calls: Low-Frequency centered around 41 kHz and High-Frequency centered around 60 kHz, but in different percentages. Between populations, only Low-Frequency calls were shorter and higher-frequency in Mongolian P. campbelli. Between species, only High-Frequency calls were shorter and higher-frequency in P. sungorus. In hybrids, Low-Frequency calls were shorter and lower-frequency than in either parental species, whereas High-Frequency calls were longer and lower-frequency in hybrids than in pure P. sungorus but similar with another parental species. We discuss that interspecific hybridization may give rise to offspring with new properties of ultrasonic calls.

Measuring airborne components of seismic body vibrations in a Middle-Asian sand-dwelling Insectivora species, the piebald shrew (Diplomesodon pulchellum).

Self-produced seismic vibrations have been found for some subterranean rodents but have not been reported for any Insectivora species, although seismic sensitivity has been confirmed for blind sand-dwelling chrysochlorid golden moles. Studying the vocal behaviour of captive piebald shrews, Diplomesodon pulchellum, we documented vibrations, apparently generated by the whole-body wall muscles, from 11 (5 male, 6 female) of 19 animals, placed singly on a drum membrane. The airborne waves of the vibratory drumming were digitally recorded and then analysed spectrographically. The mean frequency of vibration was 160.5 Hz. This frequency matched the periodicity of the deep sinusoidal frequency modulation (159.4 Hz) found in loud screech calls of the same subjects. The body vibration was not related to thermoregulation, hunger-related depletion of energy resources or fear, as it was produced by well-fed, calm animals, at warm ambient temperatures. We hypothesize that in the solitary, nocturnal, digging desert piebald shrew, body vibrations may be used for seismic exploration of substrate density, to avoid energy-costly digging of packed sand for burrowing and foraging. At the same time, the piercing quality of screech calls due to the deep sinusoidal frequency modulation, matching the periodicity of body vibration, may be important for agonistic communication in this species.

The potential to encode sex, age, and individual identity in the alarm calls of three species of Marmotinae.

In addition to encoding referential information and information about the sender's motivation, mammalian alarm calls may encode information about other attributes of the sender, providing the potential for recognition among kin, mates, and neighbors. Here, we examined 96 speckled ground squirrels (Spermophilus suslicus), 100 yellow ground squirrels (Spermophilus fulvus) and 85 yellow-bellied marmots (Marmota flaviventris) to determine whether their alarm calls differed between species in their ability to encode information about the caller's sex, age, and identity. Alarm calls were elicited by approaching individually identified animals in live-traps. We assume this experimental design modeled a naturally occurring predatory event, when receivers should acquire information about attributes of a caller from a single bout of alarm calls. In each species, variation that allows identification of the caller's identity was greater than variation allowing identification of age or sex. We discuss these results in relation to each species' biology and sociality.

Developmental changes of nasal and oral calls in the goitred gazelle Gazella subgutturosa, a nonhuman mammal with a sexually dimorphic and descended larynx.

In goitred gazelles (Gazella subgutturosa), sexual dimorphism of larynx size and position is reminiscent of the case in humans, suggesting shared features of vocal ontogenesis in both species. This study investigates the ontogeny of nasal and oral calls in 23 (10 male and 13 female) individually identified goitred gazelles from shortly after birth up to adolescence. The fundamental frequency (f0) and formants were measured as the acoustic correlates of the developing sexual dimorphism. Settings for LPC analysis of formants were based on anatomical dissections of 5 specimens. Along ontogenesis, compared to females, male f0 was consistently lower both in oral and nasal calls and male formants were lower in oral calls, whereas the first two formants of nasal calls did not differ between sexes. In goitred gazelles, significant sex differences in f0 and formants appeared as early as the second week of life, while in humans they emerge only before puberty. This result suggests different pathways of vocal ontogenesis in the goitred gazelles and in humans.

Polyphony of domestic dog whines and vocal cues to body size.

In domestic dogs Canis familiaris, vocal traits have been investigated for barks and growls, and the relationship between individual body size and vocal traits investigated for growls, with less corresponding information for whines. In this study, we examined the frequency and temporal traits of whines of 20 adult companion dogs (9 males, 11 females), ranging in body mass from 3.5 to 70.0 kg and belonging to 16 breeds. Dog whines (26-71 per individual, 824 in total) were recorded in conditioned begging contexts modeled by dog owners. Whines had 3 independent fundamental frequencies: the low, the high and the ultra-high that occurred singly as monophonic calls or simultaneously as 2-voice biphonic or 3-voice polyphonic calls. From the smallest to largest dog, the upper frequency limit varied from 0.24 to 2.13 kHz for the low fundamental frequency, from 2.95 to 10.46 kHz for the high fundamental frequency and from 9.99 to 23.26 kHz for the ultra-high fundamental frequency. Within individuals, the low fundamental frequency was lower in monophonic than in biphonic whines, whereas the high fundamental frequency did not differ between those whine types. All frequency variables of the low, high, and ultra-high fundamental frequencies correlated negatively with dog body mass. For duration, no correlation with body mass was found. We discuss potential production mechanisms and sound sources for each fundamental frequency; point to the acoustic similarity between high-frequency dog whines and rodent ultrasonic calls and hypothesize that ultra-high fundamental frequencies function to allow private, "tete-a-tete" communication between members of social groups.

Ontogeny of audible squeaks in yellow steppe lemming Eolagurus luteus: trend towards shorter and low-frequency calls is reminiscent of those in ultrasonic vocalization.

BACKGROUND: Rodents are thought to be produced their human-audible calls (AUDs, below 20 kHz) with phonation mechanism based on vibration of the vocal folds, whereas their ultrasonic vocalizations (USVs, over 20 kHz) are produced with aerodynamic whistle mechanism. Despite of different production mechanisms, the acoustic parameters (duration and fundamental frequency) of AUDs and USVs change in the same direction along ontogeny in collared lemming Dicrostonyx groenlandicus and fat-tailed gerbil Pachyuromys duprasi. We hypothesize that this unidirectional trend of AUDs and USVs is a common rule in rodents and test whether the AUDs of yellow steppe lemmings Eolagurus luteus would display the same ontogenetic trajectory (towards shorter and low-frequency calls) as their USVs, studied previously in the same laboratory colony. RESULTS: We examined for acoustic variables 1200 audible squeaks emitted during 480-s isolation-and-handling procedure by 120 individual yellow steppe lemmings (at 12 age classes from neonates to breeding adults, 10 individuals per age class, up to 10 calls per individual, each individual tested once). We found that the ontogenetic pathway of the audible squeaks, towards shorter and lower frequency calls, was the same as the pathway of USVs revealed during 120-s isolation procedure in a previous study in the same laboratory population. Developmental milestone for the appearance of mature patterns of the squeaks (coinciding with eyes opening at 9-12 days of age), was the same as previously documented for USVs. Similar with ontogeny of USVs, the chevron-like squeaks were prevalent in neonates whereas the squeaks with upward contour were prevalent after the eyes opening. CONCLUSION: This study confirms a hypothesis of common ontogenetic trajectory of call duration and fundamental frequency for AUDs and USVs within species in rodents. This ontogenetic trajectory is not uniform across species.

Non-individualistic ultrasonic and audible isolation calls throughout ontogeny in a rodent, Eolagurus luteus.

Acoustic individuality is present in diverse taxa of mammals and birds, becoming especially prominent in those age groups for which discriminating conspecifics by voice is critically important. This study compares, for the first time, the ontogenetic changes of acoustic individuality of ultrasonic and audible calls (USVs and AUDs) across 12 age-classes (from neonates to adults) in captive yellow steppe lemmings Eolagurus luteus. We found that, in this rodent species, the isolation-induced USVs and AUDs are not individually distinct at any age. We discuss that this result is unusual, because discriminating individuals by individualistic vocal traits may be important for such a social species as yellow steppe lemming. We also discuss the potential role of acoustic individuality in studies including rodent models.

Pup ultrasonic isolation calls of six gerbil species and the relationship between acoustic traits and body size.

Among Gerbillinae rodents, ultrasonic calls of adults of small-sized species are typically higher frequency than those of adults of large-sized species. This study investigates whether a similar relationship can be found in pups of six gerbil species (Dipodillus campestris, Gerbillus perpallidus, Meriones unguiculatus, Meriones vinogradovi, Sekeetamys calurus and Pachyuromys duprasi). We compared the average values of acoustic variables (duration, fundamental and peak frequency) of ultrasonic calls (20 calls per pup, 1200 in total) recorded from 6- to 10-day-old pups (10 pups per species, 60 in total) isolated for 2 min at 22°C and then weighed and measured for body variables. The longest calls (56 ± 33 ms) were found in the largest species, and the highest frequency calls (74.8 ± 5.59 kHz) were found in the smallest species. However, across species, call duration (ranging from 56 to 159 ms among species) did not display a significant relationship with pup body size; and, among frequency variables, only the minimum fundamental frequency depended on pup body size. Discriminant analysis assigned 100% of calls to the correct species. The effect of species identity on the acoustics was stronger than the effect of body size. We discuss these results with the hypotheses of acoustic adaptation, social complexity, hearing ranges and phylogeny.

Discomfort-related changes of call rate and acoustic variables of ultrasonic vocalizations in adult yellow steppe lemmings Eolagurus luteus.

Potential of ultrasonic vocalizations (USVs) to reflect a degree of discomfort of a caller is mostly investigated in laboratory rats and mice but poorly known in other rodents. We examined 36 (19 male, 17 female) adult yellow steppe lemmings Eolagurus luteus for presence of USVs during 8-min experimental trials including 2-min test stages of increasing discomfort: isolation, touch, handling and body measure. We found that 33 of 36 individuals vocalized at isolation stage, i.e., without any human impact. For 14 (6 male and 8 female) individuals, a repeated measures approach revealed that increasing discomfort from isolation to handling stages resulted in increase of call power quartiles and fundamental frequency, whereas call rate remained unchanged. We discuss that, in adult yellow steppe lemmings, the discomfort-related changes of USV fundamental frequency and power variables follow the same common rule as the audible calls of most mammals, whereas call rate shows a different trend. These data contribute to research focused on searching the universal acoustic cues to discomfort in mammalian USVs.

Vocal and physical phenotypes of calsyntenin2 knockout mouse pups model early-life symptoms of the autism spectrum disorder.

This study discovered a novel acoustic phenotype in Calsyntenin2 deficient knockout (Clstn2-KO) pups in the neurodevelopment period of 5-9 postnatal days (PND 5-9). The narrowband ultrasonic calls (nUSVs) were less complex (mostly one-note, shorter in duration and higher in peak frequency) in Clsnt2-KO than in wild-type (WT) C57BL/6 J pups. The wideband ultrasonic calls (wUSVs) were produced substantially more often by Clstn2-KO than WT pups. The clicks were longer in duration and higher in peak frequency and power quartiles in Clstn2-KO pups. The elevated discomfort due to additional two-minute maternal separation coupled with experimenter's touch, resulted in significantly higher call rates of both nUSVs and clicks in pups of both genotypes and sexes compared to the previous two-minute maternal separation, whereas the call rate of wUSVs was not affected. In Clstn2-KO pups, the prevalence of emission of wUSVs retained at both sex and both degrees of discomfort, thus providing a reliable quantitative acoustic indicator for this genetic line. Besides the acoustic differences, we also detected the increased head-to-body ratio in Clstn2-KO pups. Altogether, this study demonstrated that lack of such synaptic adhesion protein as calsyntenin2 affects neurodevelopment of vocalization in a mouse as a model organism.

Stability of acoustic individuality in the alarm calls of wild yellow ground squirrels Spermophilus fulvus and contrasting calls from trapped and free-ranging callers.

The questions of individuality and stability of cues to identity in vocal signals are of considerable importance from theoretical and conservation perspectives. While individuality in alarm calls has been reported for many sciurids, it is not well-documented that the vocal identity encoded in the alarm calls is stable between different encounters with predators. Previous studies of two obligate hibernating rodents, speckled ground squirrels Spermophilus suslicus, and yellow ground squirrels Spermophilus fulvus demonstrated that, after hibernation, most individuals could not be identified reliably by their alarm calls. Moreover, in most speckled ground squirrels, individual patterns of alarm calls changed progressively over as little as 2 weeks. However, these previous data have been obtained using the collection of alarm calls from trapped animals. Here, we examined ten free-ranging dye-marked yellow ground squirrels to determine whether their alarm calls retain the cues to individuality between two encounters of surrogate predators (humans), separated on average by 3 days. Discriminant function analysis showed that the alarm calls of individual yellow ground squirrels were very similar within a recording session, providing very high individual distinctiveness. However, in six of the ten animals, the alarm calls were unstable between recording sessions. Also, we examined ten dye-marked individuals for consistency of acoustic characteristics of their alarm calls between the encounters of humans, differing in techniques of call collection, from free-ranging vs trapped animals. We found differences only in two variables, both related to sound degradation in the environment. Data are discussed in relation to hypotheses explaining the adaptive utility of acoustic individuality in alarm calls.