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Environ Microbiol ; 10(2): 339-53, 2008 Feb.
Artigo em Inglês | MEDLINE | ID: mdl-17903215

RESUMO

On a global basis, peatlands are a major reserve of carbon (C). Hydrological changes can affect the decomposition processes in peatlands and in turn can alter their C balance. Since 1959, a groundwater extraction plant has generated a water-level gradient at our study site that has gradually changed part of the wet fen into a dry peatland forest. The average water-level drawdown of the gradient (from a pristine 9 cm to 26 cm in the dry end) is close to an estimate predicted by an increase in mean global temperature of 3 degrees C. We studied the total microbial community of the aerobic surface peat in four locations along the gradient through phospholipid fatty acid and PCR-DGGE methods. Additionally, field measurements of soil respiration showed a threefold increase in the C-emission rate at the driest location compared with the wettest one, indicating enhanced decomposition. Also, both fungal and bacterial biomass increased in the drier locations. At the species level, the fungal community changed due to water-level drawdown whereas actinobacteria were less sensitive to drying. The majority of fungal sequences were similar to ectomycorrhizal (ECM) fungi, which dominated throughout the gradient. Our results indicate that ECM fungi might act as important facultative decomposers in organic-rich environments such as peatlands.


Assuntos
Actinobacteria/crescimento & desenvolvimento , Ecossistema , Fungos/crescimento & desenvolvimento , Consumo de Oxigênio , Microbiologia do Solo , Solo/análise , Actinobacteria/classificação , Actinobacteria/genética , Actinobacteria/isolamento & purificação , Aerobiose , DNA Bacteriano/análise , DNA Fúngico/análise , DNA Espaçador Ribossômico/análise , Eletroforese em Gel de Ágar , Ácidos Graxos/análise , Fungos/classificação , Fungos/genética , Fungos/isolamento & purificação , Dados de Sequência Molecular , Fosfolipídeos/análise , Reação em Cadeia da Polimerase , Análise de Componente Principal , RNA Ribossômico 16S/genética , Análise de Sequência de DNA , Água
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