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RATIONALE: Exposure to particulate matter is associated with risk of cardiovascular events, possibly through endothelial dysfunction, and indoor air may be most important. OBJECTIVES: We investigated effects of controlled exposure to indoor air particles on microvascular function (MVF) as the primary endpoint and biomarkers of inflammation and oxidative stress as secondary endpoints in a healthy elderly population. METHODS: A total of 21 nonsmoking couples participated in a randomized, double-blind, crossover study with two consecutive 48-hour exposures to either particle-filtered or nonfiltered air (2,533-4,058 and 7,718-12,988 particles/cm(3), respectively) in their homes. MEASUREMENTS AND MAIN RESULTS: MVF was assessed noninvasively by measuring digital peripheral artery tone after arm ischemia. Secondary endpoints included hemoglobin, red blood cells, platelet count, coagulation factors, P-selectin, plasma amyloid A, C-reactive protein, fibrinogen, IL-6, tumor necrosis factor-alpha, protein oxidation measured as 2-aminoadipic semialdehyde in plasma, urinary 8-iso-prostaglandin F(2alpha), and blood pressure. Indoor air filtration significantly improved MVF by 8.1% (95% confidence interval, 0.4-16.3%), and the particulate matter (diameter < 2.5 mum) mass of the indoor particles was more important than the total number concentration (10-700 nm) for these effects. MVF was significantly associated with personal exposure to iron, potassium, copper, zinc, arsenic, and lead in the fine fraction. After Bonferroni correction, none of the secondary biomarkers changed significantly. CONCLUSIONS: Reduction of particle exposure by filtration of recirculated indoor air for only 48 hours improved MVF in healthy elderly citizens, suggesting that this may be a feasible way of reducing the risk of cardiovascular disease.
Assuntos
Poluição do Ar em Ambientes Fechados/prevenção & controle , Aterosclerose/prevenção & controle , Monitoramento Ambiental , Filtros Microporos , Material Particulado/toxicidade , Vasculite/prevenção & controle , Administração por Inalação , Fatores Etários , Idoso , Poluição do Ar em Ambientes Fechados/análise , Aterosclerose/sangue , Aterosclerose/etiologia , Aterosclerose/patologia , Intervalos de Confiança , Estudos Cross-Over , Método Duplo-Cego , Feminino , Filtração/métodos , Humanos , Mediadores da Inflamação/sangue , Exposição por Inalação , Masculino , Pessoa de Meia-Idade , Dióxido de Nitrogênio/análise , Estresse Oxidativo , Valor Preditivo dos Testes , Valores de Referência , Medição de Risco , Sensibilidade e Especificidade , Fatores Sexuais , Vasculite/sangue , Vasculite/etiologiaRESUMO
Particulate air pollution is associated with increased risk of pulmonary diseases and detrimental outcomes related to the cardiovascular system, including altered vessel functions. This study's objective was too evaluate the effects of ambient particle exposure on the blood-gas permeability, lung function and Clara cell 16 (CC16) protein release in healthy young subjects. Twenty-nine nonsmokers participated in a randomized, two-factor crossover study with or without biking exercise for 180 min and with 24-h exposure to particle-rich (6169-15,362 particles/cm(3); 7.0-11.6 microg/m(3) PM(2.5); 7.5-15.8 microg/m(3) PM(10-2.5)) or filtered (91-542 particles/cm(3)) air collected above a busy street. The clearance rate of aerosolized (99m)Tc-labeled diethylenetriamine pentaacetic acid ((99m)Tc-DTPA) was measured as an index for the alveolar epithelial membrane integrity and permeability of the lung blood-gas barrier after rush-hour exposure. Lung function was assessed using body plethysmography, flow-volume curves, and measurements of the diffusion capacity of carbon monoxide. CC16 was measured in plasma and urine as another marker of alveolar integrity. Particulate matter exposure had no significant effect on the epithelial membrane integrity using the methods available in this study. Exercise increased the clearance rate of (99m)Tc-DTPA indicated by a 6.8% (95% CI: 0.4-12.8%) shorter half-life and this was more pronounced in men than women. Neither particulate matter exposure nor exercise had an effect on the concentration of CC16 in plasma and urine or on the static and dynamic volumes or ventilation distribution of the lungs. The study thus demonstrates increased permeability of the alveolar blood-gas barrier following moderate exercise, whereas exposure to ambient levels of urban air particles has no detectable effects on the alveolar blood-gas barrier or lung function.
Assuntos
Barreira Alveolocapilar/efeitos dos fármacos , Permeabilidade da Membrana Celular/efeitos dos fármacos , Exposição Ambiental , Pulmão/efeitos dos fármacos , Pulmão/fisiologia , Material Particulado/análise , Adulto , Ar/análise , Ciclismo , Estudos Cross-Over , Interpretação Estatística de Dados , Feminino , Meia-Vida , Humanos , Masculino , Taxa de Depuração Metabólica/fisiologia , Tamanho da Partícula , Alvéolos Pulmonares/citologia , Alvéolos Pulmonares/fisiologia , Testes de Função Respiratória/métodos , Fatores Sexuais , Software , Manejo de Espécimes/métodos , Pentetato de Tecnécio Tc 99m/administração & dosagem , Pentetato de Tecnécio Tc 99m/metabolismo , Uteroglobina/sangue , Uteroglobina/urinaRESUMO
BACKGROUND: Particulate air pollution is associated with increased risk of cardiovascular events although the involved mechanisms are poorly understood. The objective of the present study was to investigate the effects of controlled exposure to ambient air fine and ultrafine particles on microvascular function and biomarkers related to inflammation, haemostasis and lipid and protein oxidation. METHODS: Twenty-nine subjects participated in a randomized, two-factor crossover study with or without biking exercise for 180 minutes and with 24 hour exposure to particle rich (number concentrations, NC: 11600 +/- 5600 per cm3, mass concentrations: 13.8 +/- 7.4 mug/m3 and 10.5 +/- 4.8 mug/m3 for PM10-2.5 and PM2.5, respectively) or particle filtered (NC: 555 +/- 1053 per cm3) air collected above a busy street. Microvascular function was assessed non-invasively by measuring digital peripheral artery tone following arm ischemia. Biomarkers included haemoglobin, red blood cells, platelet count, coagulation factors, C-reactive protein, fibrinogen, interleukin-6, tumour necrosis factor alpha, lag time to copper-induced oxidation of plasma lipids and protein oxidation measured as 2-aminoadipic semialdehyde in plasma. RESULTS: No statistically significant differences were observed on microvascular function or the biomarkers after exposure to particle rich or particle filtered air. CONCLUSION: This study indicates that exposure to air pollution particles at outdoor concentrations is not associated with detectable systemic inflammation, lipid or protein oxidation, altered haemostasis or microvascular function in young healthy participants.
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BACKGROUND: Particulate matter, especially ultrafine particles (UFPs), may cause health effects through generation of oxidative stress, with resulting damage to DNA and other macromolecules. OBJECTIVE: We investigated oxidative damage to DNA and related repair capacity in peripheral blood mononuclear cells (PBMCs) during controlled exposure to urban air particles with assignment of number concentration (NC) to four size modes with average diameters of 12, 23, 57, and 212 nm. DESIGN: Twenty-nine healthy adults participated in a randomized, two-factor cross-over study with or without biking exercise for 180 min and with exposure to particles (NC 6169-15362/cm(3)) or filtered air (NC 91-542/cm(3)) for 24 hr. METHODS: The levels of DNA strand breaks (SBs), oxidized purines as formamidopyrimidine DNA glycolase (FPG) sites, and activity of 7,8-dihydro-8-oxoguanine-DNA glycosylase (OGG1) in PBMCs were measured by the Comet assay. mRNA levels of OGG1, nucleoside diphosphate linked moiety X-type motif 1 (NUDT1), and heme oxygenase-1 (HO1) were determined by real-time reverse transcriptase-polymerase chain reaction. RESULTS: Exposure to UFPs for 6 and 24 hr significantly increased the levels of SBs and FPG sites, with a further insignificant increase after physical exercise. The OGG1 activity and expression of OGG1, NUDT1, and HO1 were unaltered. There was a significant dose-response relationship between NC and DNA damage, with the 57-nm mode as the major contributor to effects. Concomitant exposure to ozone, nitrogen oxides, and carbon monoxide had no influence. CONCLUSION: Our results indicate that UFPs, especially the 57-nm soot fraction from vehicle emissions, causes systemic oxidative stress with damage to DNA and no apparent compensatory up-regulation of DNA repair within 24 hr.
Assuntos
Poluentes Atmosféricos/toxicidade , Dano ao DNA , Leucócitos Mononucleares/efeitos dos fármacos , Estresse Oxidativo , Material Particulado/toxicidade , Adulto , Monóxido de Carbono/toxicidade , Estudos Cross-Over , DNA Glicosilases/genética , DNA Glicosilases/metabolismo , Reparo do DNA , Enzimas Reparadoras do DNA/genética , Exercício Físico , Feminino , Guanina/metabolismo , Heme Oxigenase-1/genética , Humanos , Leucócitos Mononucleares/metabolismo , Masculino , Óxidos de Nitrogênio/toxicidade , Ozônio/toxicidade , Tamanho da Partícula , Monoéster Fosfórico Hidrolases/genética , RNA Mensageiro/metabolismo , Emissões de Veículos/toxicidadeRESUMO
This review is based on the results obtained from the Danish AMAP programme for the Arctic atmosphere during the 1990s. The purpose of the programme is to quantify the pollution, apportion source contributions, follow the trends, and identify midlatitude source areas and transport pathways. The project has been carried out in North Greenland as integrated monitoring, which is an interacting combination of field measurements and model calculations of atmospheric transport and transformation in the Northern Hemisphere. At the monitoring site at Station Nord the large and seasonally recurrent variations in the pollutant concentrations are testimony to the influence in this region of the phenomenon of Arctic Haze. These results can only be understood in terms of long range transport from distant pollution sources. The measurements also comprise a large number of particle-born elements. These results are used to build receptor models, which show that the ambient concentrations and their variations to a high degree can be explained by the influence of only four source types of both natural and anthropogenic nature. The challenging phenomena of atmospheric ozone and mercury depletion around Polar sunrise have been studied at Station Nord over several years. The results show that these two phenomena are closely connected, presumably through photochemical reactions with atmospheric halogens released from sea ice. A large-scale Eulerian model system for the Northern Hemisphere has been developed in this AMAP project. The validity of the model is illustrated by comparisons between measured and calculated air concentrations. The model has been used to calculate both the vertical distribution and the atmospheric depositions for several pollutants at various locations in Greenland and split into quantified contributions from different and geographically distant source areas. Mercury deposition estimates for the Northern Hemisphere are also presented. They show that the mercury depletion events are accompanied by very intense depositions to land and sea of reactive mercury that may seriously affect the Arctic ecosystems. Finally, measurement and model data are combined to demonstrate, despite considerable meteorological noise, that ambient air concentrations in North-eastern Greenland have decreased during the last decade. Quantified trends, attributable to emission reductions in distant source areas, are presented for several pollutants.
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Poluentes Atmosféricos/análise , Ecossistema , Mercúrio/análise , Modelos Teóricos , Movimentos do Ar , Poluentes Atmosféricos/efeitos adversos , Animais , Regiões Árticas , Groenlândia , Mercúrio/efeitos adversosRESUMO
Epidemiological studies have consistently shown relation between exposure to particulate matter in urban air and cardiovascular and pulmonary morbidity and mortality. Some recent population studies suggest that the ultrafine fraction of particulate matter generated by motorised vehicles is particularly damaging. Animal studies and cell culture experiments support this hypothesis and address the possible mechanisms of action. Inhalation of particulate matter, especially ultrafine particles, causes oxidative stress and inflammation in lung tissue, which affects the lung cells per se, as well as the cardiovascular system.
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Poluentes Atmosféricos/efeitos adversos , Doenças Cardiovasculares/mortalidade , Pneumopatias/mortalidade , Emissões de Veículos/efeitos adversos , Animais , Doenças Cardiovasculares/etiologia , Humanos , Pneumopatias/etiologia , Tamanho da Partícula , Pneumonia/etiologia , Pneumonia/mortalidade , Fatores de RiscoRESUMO
INTRODUCTION: Ambient air pollution is a risk factor for mortality and morbidity. The aim of this study was to quantify the health effects related to particulate matter (PM10) in Denmark. MATERIALS AND METHODS: We used relative risk estimates in relation to PM10 based on the epidemiological literature. Population exposure to PM10 was calculated. Cases attributable to PM10 were estimated for mortality, cardiovascular and respiratory hospital admissions, chronic bronchitis, acute bronchitis, restricted activity days, and asthma attacks. Moreover, we attempted to estimate health-related gains from equipping all heavy-duty vehicles in Denmark with particle filters. RESULTS: We estimated the average population PM10 exposure to be about 22 micrograms/m3, about one third of which can be attributed to natural (not man-made) PM10. The number of cases per year attributable to the estimated exposure included about 5,000 deaths, about 5,000 hospital admissions, about 5,000 cases of chronic bronchitis, about 17,000 cases of acute bronchitis, about 200,000 asthma attacks, and about three million restricted activity days. The health-related gains from installing particle filters on all heavy-duty vehicles in Denmark are uncertain; the estimates for mortality ranged from 22 to 1,250, depending on the assumptions. DISCUSSION: Although air pollution constitutes only a minor risk factor on the individual level, it seems to be a major public health problem. The particle characteristics responsible for the estimated health effects are not well understood. The ultrafine particle fraction may cause a much greater impact on health than indicated by the mass.
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Poluentes Atmosféricos/efeitos adversos , Doenças Cardiovasculares/etiologia , Exposição Ambiental/efeitos adversos , Doenças Respiratórias/etiologia , Medição de Risco/métodos , Adolescente , Adulto , Asma/epidemiologia , Asma/etiologia , Asma/mortalidade , Bronquite/epidemiologia , Bronquite/etiologia , Bronquite/mortalidade , Doenças Cardiovasculares/epidemiologia , Doenças Cardiovasculares/mortalidade , Criança , Dinamarca/epidemiologia , Feminino , Humanos , Masculino , Tamanho da Partícula , Doenças Respiratórias/epidemiologia , Doenças Respiratórias/mortalidadeRESUMO
The present investigation, carried out as a case study in a typical major city situated in a European coal combustion region (Krakow, Poland), aims at quantifying the impact on the urban air quality of residential heating by coal combustion in comparison with other potential pollution sources such as power plants, industry, and traffic. Emissions were measured for 20 major sources, including small stoves and boilers, and the particulate matter (PM) was analyzed for 52 individual compounds together with outdoor and indoor PM10 collected during typical winter pollution episodes. The data were analyzed using chemical mass balance modeling (CMB) and constrained positive matrix factorization (CMF) yielding source apportionments for PM10, B(a)P, and other regulated air pollutants namely Cd, Ni, As, and Pb. The results are potentially very useful for planning abatement strategies in all areas of the world, where coal combustion in small appliances is significant. During the studied pollution episodes in Krakow, European air quality limits were exceeded with up to a factor 8 for PM10 and up to a factor 200 for B(a)P. The levels of these air pollutants were accompanied by high concentrations of azaarenes, known markers for inefficient coal combustion. The major culprit for the extreme pollution levels was demonstrated to be residential heating by coal combustion in small stoves and boilers (>50% for PM10 and >90% B(a)P), whereas road transport (<10% for PM10 and <3% for B(a)P), and industry (4-15% for PM10 and <6% for B(a)P) played a lesser role. The indoor PM10 and B(a)P concentrations were at high levels similar to those of outdoor concentrations and were found to have the same sources as outdoors. The inorganic secondary aerosol component of PM10 amounted to around 30%, which for a large part may be attributed to the industrial emission of the precursors SO2 and NOx.
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Poluentes Atmosféricos/análise , Arsênio/análise , Calefação/efeitos adversos , Metais Pesados/análise , Material Particulado/análise , Poluição do Ar/análise , Carvão Mineral , Habitação , Polônia , UrbanizaçãoRESUMO
An association between particulate air pollution and morbidity and mortality is well established. However, little is known about which sources of particulate matter contribute most to the adverse health effects. Identification of responsible sources would merit more efficient control. For a 6-year period (01 January 1999 to 31 December 2004), we examined associations between urban background PM(10) in the presence of gaseous pollutants (CO, NO(2)) and hospital admissions due to cardiovascular and respiratory disease in the elderly (age>/=65), and asthma in children (age 5-18) in Copenhagen, Denmark. We further studied associations between fractions of PM(10) assigned to six sources (biomass, secondary, oil, crustal, sea salt, and vehicle) and admissions during a 1(1/2) -year campaign. We used Poisson generalized additive time-series model adjusted for season, day of the week, public holidays, influenza epidemics, grass pollen, school holidays, and meteorology, with up to 5 days lagged air pollution exposure. We found positive associations between PM(10) and the three health outcomes, with strongest associations for asthma. The PM(10) effect remained robust in the presence of CO and NO(2). We found different PM(10) sources to be variably associated with different outcomes: crustal and secondary sources showed strongest associations with cardiovascular, biomass with respiratory, and vehicle with asthma admissions. These novel results may merit future research of potential mechanism, whereas at present, no single PM(10) source can be attributed to all morbidity.
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Poluição do Ar em Ambientes Fechados/análise , Exposição Ambiental/análise , Hospitalização/estatística & dados numéricos , Adolescente , Idoso , Poluição do Ar em Ambientes Fechados/efeitos adversos , Asma/epidemiologia , Asma/etiologia , Criança , Pré-Escolar , Dinamarca/epidemiologia , Exposição Ambiental/efeitos adversos , Humanos , Análise de Componente Principal , Doenças Respiratórias/epidemiologia , Doenças Respiratórias/etiologia , Medição de RiscoRESUMO
Atmospheric mercury depletion episodes (AMDEs) were studied at Station Nord, Northeast Greenland, 81 degrees 36' N, 16 degrees 40' W, during the Arctic Spring. Gaseous elemental mercury (GEM) and ozone were measured starting from 1998 and 1999, respectively, until August 2002. GEM was measured with a TEKRAN 2735A automatic mercury analyzer based on preconcentration of mercury on a gold trap followed by detection using fluorescence spectroscopy. Ozone was measured by UV absorption. A scatter plot of GEM and ozone concentrations confirmed that also at Station Nord GEM and ozone are linearly correlated during AMDEs. The relationship between ozone and GEM is further investigated in this paper using basic reaction kinetics (i.e., Cl, ClO, Br, and BrO have been suggested as reactants for GEM). The analyses in this paper show that GEM in the Arctic troposphere most probably reacts with Br. On the basis of the experimental results of this paper and results from the literature, a simple parametrization for AMDE was included into the Danish Eulerian Hemispheric Model (DEHM). In the model, GEM is converted linearly to reactive gaseous mercury (RGM) over sea ice with temperature below -4 degrees C with a lifetime of 3 or 10 h. The new AMDE parametrization was used together with the general parametrization of mercury chemistry [Petersen, G.; Munthe, J.; Pleijel, K.; Bloxam, R.; Vinod Kumar, A. Atmos. Environ. 1998, 32, 829-843]. The obtained model results were compared with measurements of GEM at Station Nord. There was good agreement between the start and general features periods with AMDEs, although the model could not reproduce the fast concentration changes, and the correlation between modeled and measured values decreased from 2000 to 2001 and further in 2002. The modeled RGM concentrations over the Arctic in 2000 were found to agree well with the temporal and geographical variability of the boundary column of monthly average BrO observed by the GOME satellite. Scenario calculations were performed with and without AMDEs. For the area north of the Polar Circle, the mercury deposition increases from 89 tons/year for calculations without an AMDE to 208 tons/year with the AMDE. The 208 tons/year represent an upper limit for the mercury load to the Artic.