RESUMO
Microalgae produce increased lipid content accompanied by a significant decrease in cell density with decreasing nitrate concentration. Magnetic fields (MF) have been reported as a factor that could accelerate metabolism and growth in microalgae culture. Thus, this study aimed to optimize the influence of MF and nitrate concentration (sodium nitrate, N) on the growth and lipid productivity of Nannochloropsis oculata. A single-factor experiment integrated with response surface methodology (RSM) via central composite design (CCD) was performed. The results showed that the maximum specific growth rate (0.24 d-1) and maximum lipid productivity (38â¯mgâ¯L-1 d-1) obtained in this study were higher than those of the control culture (by 166% and 103%, respectively). This study also found that the two-way interaction term MFâ¯×â¯N had a significant effect on cell growth but not on lipid production. It was concluded that to design appropriate MF for enhanced lipid productivity due to cell growth, further research must focus on developing an understanding of the relationship between the bioeffects of the magnetic field and the proteomic changes involved in lipid accumulation strategies. This approach would enable the design of conditions to obtain inexpensive high-value products from N. oculata.
Assuntos
Microalgas , Estramenópilas , Biomassa , Lipídeos , Campos Magnéticos , ProteômicaRESUMO
Hearing-protection devices (HPDs) are particularly important in protecting the hearing of workers. The aim of this study was to prevent hearing damage in workplaces in Taiwan. It was conducted to determine the actual sound attenuation of the personal attenuation rating (PAR) values when wearing HPDs via measurements from field microphones in workers' real ears (F-MIRE). Across 105 measurement trials for the Classic™ roll-down foam earplug HPDs worn by the workers, there were 23 cases of ineffective protection (including caution and fail); the proportion was 20% (including the first measurement and re-wear of HPDs after education and training). In addition, re-education and training in how to wear the HPDs was provided, improving wearing skills. A total of 29 testees wearing the Classic™ roll-down foam earplug HPDs failed to meet the pass standard for the first PAR test, and 6 of them improved and subsequently passed the PAR test. The improvement rate was 20%. These 23 testees switched to another HPD, namely Kneading-Free Push-Ins™ earplugs. From this group, 16 effective sound attenuation values were obtained, with an improvement rate of 70%. However, seven testees failed to pass the PAR test, and after education, training, and replacement of HPDs with different types, they still could not pass the PAR test. At that time, even if the UltraFit™ pre-molded earplugs were adopted again for wear and replacement, they were still unable to pass the PAR test. This HPD was eventually replaced with the PELTOR X4A Earmuff HPD and then tested again, with these HPDs finally passing the PAR test. In Taiwan, the use of fit testing has been increasing but it is not a common practice, and few studies on hearing-protection fit testing have been conducted in this country. The goal of this study was to gain more insight into the current hearing protection situation, including field attenuation of HPDs obtained by workers, the effects of training on improving the attenuation of HPDs after F-MIRE measurements, and the awareness of hearing health and motivation on the use of HPDs in a high-noise-level environment.
Assuntos
Perda Auditiva Provocada por Ruído , Ruído Ocupacional , Exposição Ocupacional , Dispositivos de Proteção das Orelhas , Desenho de Equipamento , Humanos , TaiwanRESUMO
According to the US Bureau of Labor Statistics (BLS), 882 people were killed or injured due to confined space accidents in 2011-2017. Occupational accident statistics published in 2008-2018 by the Taiwan Occupational Safety and Health Administration (OSHA, Taiwan) show that 70 people suffered from disasters and 52 were injured in the 64 accident reports involving confined spaces. In the US, on average, 126 people die each year in accidents related to confined spaces, and in Taiwan, an average of 8 people per year are casualties of accidents involving confined spaces, proving that it is an area of concern that cannot be neglected. When misjudgments occur in confined spaces, not only can people be hurt, but they can even lose their lives, and the risks associated with confined spaces can subsequently result in rescue personnel also being killed or injured. This study was conducted via the systematic causal analysis technique (SCAT), which was proposed and developed by the International Loss Control Institute (ILCI), with the intention of identifying the critical basic causes of the confined space accidents that have occurred over the years in the Taiwan area, in order to propose corresponding improvement strategies. After investigating the statistics in Taiwan, it was determined that hydrogen sulfide was involved in 45% of accidental deaths in confined spaces, followed by 11% involving carbon dioxide, 9% involving carbon monoxide, and 7% involving toluene. Additional analysis of non-standard acts identified "failure of operating procedures" as being involved in 27% of accidents, followed by 25% involving "improper personal protective equipment" and 23% involving "incorrect position". The analysis of non-standard conditions revealed that "dangerous workplace" was involved in 39% of accidents, "improper protective measures" in 30%, and "inadequate ventilation" in 27%. In accordance with our analysis results, it could be suggested that hazard prevention strategies for confined spaces, in addition to encouraging avoidance of non-standard acts by personnel, should also strive to improve these non-standard conditions. Otherwise, if not prevented deliberately and in a fundamental, relevant accidents will remain inevitable.
Assuntos
Acidentes de Trabalho , Espaços Confinados , Saúde Ocupacional , Humanos , Taiwan , VentilaçãoRESUMO
The effect of sludge retention time (SRT) on biomass, kinetic parameters, and stoichiometric parameters of ammonia-oxidizing bacteria (AOB) and nitrite-oxidizing bacteria (NOB) in anaerobic/anoxic/oxic (A(2)O) process were explored in this study. The results showed that the growth rate constants were 1.52, 1.22, and 0.85 day(-1), respectively, for AOB, those were 1.59, 1.19, and 0.87 day(-1), respectively, for NOB when SRT was 20, 10, and 5 days. The lysis rate constants of AOB and NOB were 0.14 and 0.09 day(-1), respectively. The yield coefficients were 0.23 and 0.22, respectively, for AOB and NOB. They did not change with SRT obviously. The biomass of AOB was 50.94, 26.35, and 14.68 mg L(-1), respectively, and the biomass of NOB was 116.77, 60.00, and 44.25 mg L(-1), respectively, at SRT of 20, 10, and 5 days. When SRT diminished from 20 to 5 days, the biomass of AOB and NOB diminished by 36.26 and 75.52 mg L(-1), respectively. The removal efficiency of NH4 (+)-N diminished by 68.9 %. The removal efficiency of total nitrogen diminished by 42.9 %.
Assuntos
Bactérias/metabolismo , Biomassa , Nitrificação , Esgotos/microbiologiaRESUMO
Antibiotics are widely used in livestock for infection treatment and growth promotion. Wastes from animal husbandry are a potential environmental source of antibiotic-insensitive pathogens, and the removal efficiency of the resistance genotypes in current wastewater treatment plants (WWTPs) is unknown. In this study, quantitative PCR was used for evaluating antibiotic resistance genes in wastewater treatment processes. Six wastewater treatment plants in different swine farms were included in this study, and five antibiotic resistance genes (ARGs) were tested for each treatment procedure. All of the tested ARGs including tetA, tetW, sulI, sulII, and blaTEM genes were detected in six swine farms with considerable amounts. The results showed that antibiotic resistance is prevalent in livestock farming. The ARG levels were varied by wastewater treatment procedure, frequently with the highest level at anaerobic treatment tank and lowest in the activated sludge unit and the effluents. After normalizing the ARG levels to 16S rRNA gene copies, the results showed that ARGs in WWTP units fluctuated partly with the quantity of bacteria. Regardless of its importance in biodegradation, the anaerobic procedure may facilitate bacterial growth thus increasing the sustainability of the antibiotic resistance genotypes. After comparing the copy numbers in influx and efflux samples, the mean removal efficiency of ARGs ranged between 33.30 and 97.56%. The results suggested that treatments in the WWTP could partially reduce the spread of antibiotic-resistant bacteria, and additional procedures such as sedimentation may not critically affect the removal efficiency.
Assuntos
Criação de Animais Domésticos , Resistência Microbiana a Medicamentos/genética , Genes Bacterianos , Eliminação de Resíduos Líquidos , Águas Residuárias/microbiologia , Animais , Reação em Cadeia da Polimerase em Tempo Real , SuínosRESUMO
Backside grinding (BG) wastewater treatment typically requires large quantities of chemicals, i.e. polyaluminum chloride (PAC) coagulant and produces considerable amounts of sludge, increasing the loading and cost of subsequent sludge treatment and disposal processes. This study investigated the effects of the addition of magnetic seeds (FeO*Fe(2)O(3)) of selected particle sizes and of optimized combinations of magnetic seeds and PAC on the aggregation of silica nanoparticles from BG wastewater and on the sedimentation time at various pH values. The results show that the turbidity of BG wastewater was significantly reduced by the magnetic aggregation treatment. The dosage of PAC combined with 2.49gL(-1) or 1.24gL(-1) of magnetic seeds was reduced by 83% (from 60 to 10mgL(-1)) compared to the conventional process of using only PAC as a coagulant. The turbidity of the BG wastewater, initially 1900-2500NTU, could also be successfully decreased about to 23NTU by the addition of 3.74gL(-1) magnetite (FeO*Fe(2)O(3)) only at pH 5 with an applied magnetic field of 1000G. Different coagulation conditions using magnetic seeds combined with coagulant resulted in different aggregation performances. The treatment performance was more effective by using two-stage dosing, in which magnetic seeds and PAC were added separately, than that with one-stage dosing, where the magnetic seeds and PAC were added simultaneously during rapid mixing. The two-stage dosing allowed for a reduction in the optimum dosage of magnetic seeds from 3.74gL(-1) to 2.49gL(-1) or 1.24gL(-1) without affecting performance when coupled with 0.01gL(-1) of PAC coagulant. The developed method effectively reduced the production of waste sludge.
Assuntos
Precipitação Química , Campos Magnéticos , Magnetismo/métodos , Nanopartículas/química , Eliminação de Resíduos Líquidos , Purificação da Água/métodos , Hidróxido de Alumínio/química , Floculação , Concentração de Íons de Hidrogênio , Metais/análise , Nanopartículas/ultraestrutura , Nefelometria e Turbidimetria , Reciclagem , Eletricidade Estática , Fatores de Tempo , Qualidade da Água , Difração de Raios XRESUMO
This study compared three forecasting models based on the mean absolute percentage errors (MAPE) of their accuracy in forecasting air pollution in a traffic tunnel: the Grey model (GM), the combination model used four sample point and five sample point prediction with GM (1,1)(GM(1,1)(4 + 5)), and the modified grey model (MGM). An MGM was combined using the four points of the original sequence using the original grey prediction GM (1,1) for short-term forecasting. The proposed method cannot only enhance the prediction accuracy of the original grey model, but can also solve the jump data forecasting problem something for which the original grey model is inappropriate. The MAPE was applied to the models, and the MGM found the proposed method to be simple and efficient. The MAPE of MGM, calculated over 3 h of forecasts, were as follows: 10.12 (Upwind), 10.07 (Middle) and 7.68 (Downwind) for CO; 10.79 (Upwind), 6.05 (Middle) and 5.98 (Downwind) for NOx, and 11.67 (Upwind), 7.32 (Middle) and 4.56 (Downwind) for NMHC. The MGM model results reveal that the combined forecasts can significantly decrease the overall forecasting error. Results of this demonstrate that MGM can accurately forecast air pollution in the Kaohsiung Chung-Cheng Tunnel.
Assuntos
Poluição do Ar/análise , Ar/normas , Emissões de Veículos , Previsões , Modelos TeóricosRESUMO
This study compared three forecasting methods based on their accuracy or absolute errors in forecasting air pollution in a traffic tunnel: the Grey model (GM), the Crank-Nicholson implicit scheme model, and the forecasting combination model (FCM). Three criteria, root mean square error (RMSE), the mean absolute error (MAE) and mean absolute percentage error (MAPE), were applied to the models and the FCM model displayed all of the characteristics of a good forecasting model. The correlation coefficient (r) for the FCM model equaled 0.94 (Upwind), 0.98 (Middle) and 0.98 (Downwind). This study indicated that FCM can be used to accurately forecast CO pollution in the Kaohsiung Cross Harbor Tunnel.