Core-Shell Reactor Partitioning Enzyme and Prodrug by ZIF-8 for NADPH-Sensitive In Situ Prodrug Activation.

Enzyme-prodrug therapies have shown unique advantages in efficiency, selectivity, and specificity of in vivo prodrug activation. However, precise spatiotemporal control of both the enzyme and its substrate at the target site, preservation of enzyme activity, and in situ substrate depletion due to low prodrug delivery efficiency continue to be great challenges. Here, we propose a novel core-shell reactor partitioning enzyme and prodrug by ZIF-8, which integrates an enzyme with its substrate and increases the drug loading capacity (DLC) using a prodrug as the building ligand to form a Zn-prodrug shell. Cytochrome P450 (CYP450) is immobilized in ZIF-8, and the antitumor drug dacarbazine (DTIC) is coordinated and deposited in its outer layer with a high DLC of 43.6±0.8 %. With this configuration, a much higher prodrug conversion efficiency of CYP450 (36.5±1.5 %) and lower IC50 value (26.3±2.6 µg/mL) are measured for B16-F10 cells with a higher NADPH concentration than those of L02 cells and HUVECs. With the tumor targeting ability of hyaluronic acid, this core-shell enzyme reactor shows a high tumor suppression rate of 96.6±1.9 % and provides a simple and versatile strategy for enabling in vivo biocatalysis to be more efficient, selective, and safer.

[Study on the theory and experiments of radiative parameters of the Eu(3+) ion in transparent optical resin].

The optical properties of a transparent optical resin (HMA/ST) containing ternary rare earth complex are reported. Based on Judd-Ofelt (J-O) theory, the J-O parameters were calculated to be omega2 = 10.139 4 x 10(-20) cm2, omega4 = 3.810 9 x 10(-20) cm2, omega6 = 9.050 7 x 10(-20) cm2 by using the emission spectrum of optical resin containing Eu (TTA)3 phen-0.31 wt%. The J-O parameters were used to calculate the total radiative transition rate (456.6 s(-1)) and radiative lifetime (2 190.1 micros) of the excited state 5D0. The stimulated emission cross-sections a and the fluorescence branch ratio beta parameters for the transitions of 5D0 --> 7F(J), (where J' = 1, 2, 4 and 6) were also evaluated. By analyzing the calculated J-O parameters, it is concluded that the excited state of Eu3+ in optical resin has a long radiative lifetime and large emission cross-section, which shows that the optical resin containing rare earth complex can be used for stimulated emission amplification or as a laser material.

[Genetic Indentification of An Individual with A102Bw33 Blood Group Gene Subtypes of ABO Variant].

OBJECTIVE: To investigate and indentify the molecular characteristics of a sample serologically identified as ABw subgroup. METHODS: The individual was confirmed by standard serological techniques. The genotyping and sequencing were performed using polymerase chain reaction-sequence specific primer (PCR-SSP), direct sequencing and gene cloning for exon 6 and exon 7 of ABO locus respectively. RESULT: Both A and B antigen were detected on red blood cells of the proband and anti-B antibody was detected in his serum. PCR-SSP showed that the sample gene was A1B phemotype. DNA sequencing showed 297A/G, 467C/T, 526C/G, 657C/T, 703A/G, 803G/C and 930G/A heterozygote in exon 6 to exon 7. After cloning and sequencing, 2 alleles A102 and Bw33 were obtained. The sequence of Bw33 allele had one nucleotide change (A to C) at position 796 compared with that of B101 allele. The nucleotide in this B allele at site 796 is a "C" characteristic of the A form of the allele. CONCLUSION: A>C at nt796 of α-1, 3 galactosyltransferase gene can result in Bw33 phenotype with the anti-B antibody in serum.

[Surgical treatment of thoracic tuberculosis with one stage posterior debridement and bone grafting fusion and internal fixation].

OBJECTIVE: To investigate the effect and indication of one stage posterior debridement and bone grafting fusion and internal fixation for thoracic tuberculosis. METHODS: From January 2005 to May 2011,12 patients with thoracic tuberculosis were treated with one stage posterior debridement and pedicle screw fixation combined with regular anti-tuberculosis treatment before and after operation. There were 7 males and 5 females,with an average age of 45 years and average course of 15 months. Information of operative time, blood loss, bony fusion, local kyphosis and neurologic functional were evaluated. RESULTS: All infective focus were thoroughly removed and bone graft obtained fusion. The mean of operative time and blood loss were 170 min (120-210 min) and 510 ml (200-1 000 ml),respectively. Cobb angle from (28.7 +/- 9.2) degrees preoperatively decreased to (8.2 +/- 3.5) degrees postoperatively(P<0.05). No kyphosis correction loss,tubercular recurrence or failure of internal fixation was found. According to Frankel grade to evaluate neurological function, all patients arrived to grade E. CONCLUSION: One stage posterior debridement and bone grafting fusion and internal fixation is an effective method in treating thoracic tuberculosis. It has advantages such as thorough debridement, short operative time, less blood loss, more kyphosis correction and higher bony fusion rate.

[Expression of neutrophil adhesion molecule CD11b as an early diagnostic marker for neonatal sepsis].

OBJECTIVE: Neonatal sepsis is a common disease and the sepsis-related mortality rate is still high. Until now, there has no ideal diagnostic marker to early identify neonatal sepsis. Expression of neutrophil adhesion molecule CD(11b) was showed as the earlier reaction to the infection/inflammation, and may be applied as an early diagnostic marker for sepsis. This study was to investigate this antigen for early diagnosis of neonatal sepsis related to bacterial infection. METHODS: According to clinical symptoms, signs and four indices (WBC, PLT, plasma CRP and ratio of I/T), fifty-one neonates with established or suspected sepsis were allocated retrospectively into two groups of sepsis [n = 23, gestational age of (38.3 +/- 2.4) weeks, postnatal age of (12.7 +/- 8.8) days, body weight: (3.1 +/- 0.8) kg] and suspected sepsis [n = 28, gestational age of (38.8 +/- 1.6) weeks, postnatal age of (11.7 +/- 7.3) days, body weight: (3.3 +/- 0.6) kg]. Fifteen healthy neonates were served as controls [gestational age: (38.5 +/- 1.4) weeks, postnatal age: (8.2 +/- 5.5) days, body weight: (3.3 +/- 0.3) kg]. CD(11b) was quantified with the whole blood flow cytometry and direct immunofluorescence technique. RESULTS: The expressions of neutrophil CD(11b) in neonates with sepsis and suspected sepsis were (320 +/- 189) MFI and (456 +/- 213) MFI, respectively, which was lower than that of controls [(1,090 +/- 338) MFI, t = -9.01 and -7.56, respectively; P < 0.001]. The expression of CD(11b) was lower in neonates with sepsis than that with suspected sepsis (t = -2.39, P < 0.05). The expression of CD(11b) in neonates with CRP >or= 30 mg/L was (211 +/- 164) MFI, which was lower than those with CRP < 30 mg/L [(505 +/- 265) MFI, t = 2.64, P < 0.05]. The detection of CD(11b) (