RESUMO
Research involving human participants indicates that memories of recently eaten meals limit how much is eaten during subsequent eating episodes; yet, the brain regions that mediate the inhibitory effects of ingestion-related memory on future intake are largely unknown. We hypothesize that dorsal hippocampal (dHC) neurons, which are critical for episodic memories of personal experiences, mediate the inhibitory effects of ingestion-related memory on future intake. Our research program aimed at testing this hypothesis has been influenced in large part by our mentor James McGaugh and his research on posttraining manipulations. In the present study, we used an activity-guided optogenetic approach to test the prediction that if dHC glutamatergic neurons limit future intake through a process that requires memory consolidation, then inhibition should increase subsequent intake when given soon after the end of a meal but delayed inhibition should have no effect. Viral vectors containing CaMKIIα-eArchT3.0-eYFP and fiber optic probes were placed in the dHC of male Sprague-Dawley rats. Compared to intake on a day when no inhibition was given, postmeal inhibition of dHC glutamatergic neurons given for 10 min after the end of a saccharin meal increased the likelihood that rats would consume a second meal 90 min later and significantly increased the amount of saccharin solution consumed during that next meal when the neurons were no longer inhibited. Importantly, delayed inhibition given 80 min after the end of the saccharin meal did not affect subsequent intake of saccharin. Given that saccharin has minimal postingestive gastric consequences, these effects are not likely due to the timing of interoceptive visceral cues generated by the meal. These data show that dHC glutamatergic neural activity is necessary during the early postprandial period for limiting future intake and suggest that these neurons inhibit future intake by consolidating the memory of the preceding meal.
Assuntos
Comportamento Alimentar/fisiologia , Hipocampo/fisiologia , Memória/fisiologia , Neurônios/fisiologia , Período Pós-Prandial/fisiologia , Animais , Proteína Quinase Tipo 2 Dependente de Cálcio-Calmodulina/genética , Ácido Glutâmico/metabolismo , Interocepção , Consolidação da Memória/fisiologia , Plasticidade Neuronal/fisiologia , Optogenética , RatosRESUMO
Since epigenetic modifications are a key driver for cellular differentiation, the regulation of these modifications is tightly controlled. Interestingly, recent studies have revealed metabolic regulation for epigenetic modifications in pluripotent cells. As metabolic differences are prominent between naive (pre-implantation) and primed (post-implantation) pluripotent cells, the epigenetic changes regulated by metabolites has become an interesting topic of analysis. In this review we discuss how combinatorial metabolic activities drive the developmental progression through early pluripotent stages.
Assuntos
Blastocisto/citologia , Cromatina/genética , Células-Tronco Embrionárias/citologia , Epigênese Genética , Regulação da Expressão Gênica no Desenvolvimento , Metaboloma , Células-Tronco Pluripotentes/citologia , Animais , Blastocisto/metabolismo , Diferenciação Celular , Células-Tronco Embrionárias/metabolismo , Humanos , Células-Tronco Pluripotentes/metabolismoRESUMO
Excitotoxicity, a form of neuronal injury in which excessive activation of glutamate receptors results in cellular calcium overload, has been implicated in the pathogenesis of Alzheimer disease (AD), although direct evidence is lacking. Mutations in the presenilin-1 (PS1) gene on chromosome 14 are causally linked to many cases of early-onset inherited AD (refs. 5,6). We generated PS1 mutant mice (PS1M146VKI) that express the PS1 M146V targeted allele at normal physiological levels. Although PS1M146VKI mice have no overt mutant phenotype, they are hypersensitive to seizure-induced synaptic degeneration and necrotic neuronal death in the hippocampus. Cultured hippocampal neurons from PS1M146VKI mice have increased vulnerability to death induced by glutamate, which is correlated with perturbed calcium homeostasis, increased oxidative stress and mitochondrial dysfunction. Agents that suppress calcium influx or release and antioxidants protect neurons against the excitotoxic action of the PS1 mutation. These findings establish a direct link between a genetic defect that causes AD and excitotoxic neuronal degeneration, and indicate new avenues for therapeutic intervention in AD patients.
Assuntos
Hipocampo/citologia , Ácido Caínico/toxicidade , Proteínas de Membrana/fisiologia , Neurônios/efeitos dos fármacos , Animais , Ácido Glutâmico/farmacologia , Proteínas de Membrana/genética , Camundongos , Camundongos Endogâmicos C57BL , Camundongos Knockout , Presenilina-1RESUMO
Interleukin 7 (IL-7) stimulates the proliferation of B cell progenitors, thymocytes, and mature T cells through an interaction with a high affinity receptor (IL-7R) belonging to the hematopoietin receptor superfamily. We have further addressed the role of IL-7 and its receptor during B and T cell development by generating mice genetically deficient in IL-7R. Mutant mice display a profound reduction in thymic and peripheral lymphoid cellularity. Analyses of lymphoid progenitor populations in IL-7R-deficient mice define precisely those developmental stages affected by the mutation and reveal a critical role for IL-7R during early lymphoid development. Significantly, these studies indicate that the phase of thymocyte expansion occurring before the onset of T cell receptor gene rearrangement is critically dependent upon, and mediated by the high affinity receptor for IL-7.
Assuntos
Antígenos CD , Interleucina-7/fisiologia , Linfócitos/fisiologia , Receptores de Interleucina/fisiologia , Animais , Antígenos CD4/análise , Antígenos CD8/análise , Feminino , Leucossialina , Masculino , Camundongos , Camundongos Endogâmicos C57BL , Receptores de Interleucina/deficiência , Receptores de Interleucina-2/fisiologia , Receptores de Interleucina-7 , Sialoglicoproteínas/análiseRESUMO
To reveal how cells exit human pluripotency, we designed a CRISPR-Cas9 screen exploiting the metabolic and epigenetic differences between naïve and primed pluripotent cells. We identify the tumor suppressor, Folliculin(FLCN) as a critical gene required for the exit from human pluripotency. Here we show that FLCN Knock-out (KO) hESCs maintain the naïve pluripotent state but cannot exit the state since the critical transcription factor TFE3 remains active in the nucleus. TFE3 targets up-regulated in FLCN KO exit assay are members of Wnt pathway and ESRRB. Treatment of FLCN KO hESC with a Wnt inhibitor, but not ESRRB/FLCN double mutant, rescues the cells, allowing the exit from the naïve state. Using co-immunoprecipitation and mass spectrometry analysis we identify unique FLCN binding partners. The interactions of FLCN with components of the mTOR pathway (mTORC1 and mTORC2) reveal a mechanism of FLCN function during exit from naïve pluripotency.
Assuntos
Alvo Mecanístico do Complexo 1 de Rapamicina/metabolismo , Alvo Mecanístico do Complexo 2 de Rapamicina/metabolismo , Via de Sinalização Wnt/fisiologia , Fatores de Transcrição de Zíper de Leucina e Hélice-Alça-Hélix Básicos/genética , Fatores de Transcrição de Zíper de Leucina e Hélice-Alça-Hélix Básicos/metabolismo , Sistemas CRISPR-Cas/genética , Sistemas CRISPR-Cas/fisiologia , Linhagem Celular , Estrona/genética , Estrona/metabolismo , Humanos , Imunoprecipitação , Alvo Mecanístico do Complexo 1 de Rapamicina/genética , Alvo Mecanístico do Complexo 2 de Rapamicina/genética , Proteômica , Receptores de Estrogênio/genética , Receptores de Estrogênio/metabolismo , Via de Sinalização Wnt/genéticaRESUMO
To examine the in vivo function of presenilin-1 (PS1), we selectively deleted the PS1 gene in excitatory neurons of the adult mouse forebrain. These conditional knockout mice were viable and grew normally, but they exhibited a pronounced deficiency in enrichment-induced neurogenesis in the dentate gyrus. This reduction in neurogenesis did not result in appreciable learning deficits, indicating that addition of new neurons is not required for memory formation. However, our postlearning enrichment experiments lead us to postulate that adult dentate neurogenesis may play a role in the periodic clearance of outdated hippocampal memory traces after cortical memory consolidation, thereby ensuring that the hippocampus is continuously available to process new memories. A chronic, abnormal clearance process in the hippocampus may conceivably lead to memory disorders in the mammalian brain.
Assuntos
Precursor de Proteína beta-Amiloide/análogos & derivados , Hipocampo/crescimento & desenvolvimento , Proteínas de Membrana/deficiência , Proteínas de Membrana/genética , Memória/fisiologia , Prosencéfalo/crescimento & desenvolvimento , Doença de Alzheimer/genética , Precursor de Proteína beta-Amiloide/metabolismo , Animais , Química Encefálica/genética , Eletrofisiologia , Hipocampo/patologia , Transtornos da Memória/genética , Transtornos da Memória/patologia , Transtornos da Memória/fisiopatologia , Camundongos , Camundongos Endogâmicos C57BL , Camundongos Endogâmicos CBA , Camundongos Knockout , Camundongos Transgênicos , Neurônios/patologia , Presenilina-1 , Prosencéfalo/patologia , RNA Mensageiro/genética , RNA Mensageiro/metabolismoRESUMO
Spinocerebellar ataxia type 7 (SCA7) is an autosomal dominant disorder caused by a CAG repeat expansion. To determine the mechanism of neurotoxicity, we produced transgenic mice and observed a cone-rod dystrophy. Nuclear inclusions were present, suggesting that the disease pathway involves the nucleus. When yeast two-hybrid assays indicated that cone-rod homeobox protein (CRX) interacts with ataxin-7, we performed further studies to assess this interaction. We found that ataxin-7 and CRX colocalize and coimmunoprecipitate. We observed that polyglutamine-expanded ataxin-7 can dramatically suppress CRX transactivation. In SCA7 transgenic mice, electrophoretic mobility shift assays indicated reduced CRX binding activity, while RT-PCR analysis detected reductions in CRX-regulated genes. Our results suggest that CRX transcription interference accounts for the retinal degeneration in SCA7 and thus may provide an explanation for how cell-type specificity is achieved in this polyglutamine repeat disease.
Assuntos
Núcleo Celular/metabolismo , Proteínas de Homeodomínio/antagonistas & inibidores , Proteínas do Tecido Nervoso/fisiologia , Proteínas Nucleares/fisiologia , Peptídeos/química , Transativadores/antagonistas & inibidores , Repetições de Trinucleotídeos , Fatores Etários , Animais , Ataxina-7 , Linhagem Celular , Núcleo Celular/ultraestrutura , Modelos Animais de Doenças , Eletrorretinografia , Proteínas do Olho/química , Proteínas do Olho/genética , Proteínas do Olho/fisiologia , Perfilação da Expressão Gênica , Genes Sintéticos , Proteínas de Homeodomínio/fisiologia , Humanos , Substâncias Macromoleculares , Camundongos , Camundongos Transgênicos , Proteínas do Tecido Nervoso/química , Proteínas do Tecido Nervoso/deficiência , Proteínas do Tecido Nervoso/genética , Proteínas Nucleares/química , Proteínas Nucleares/genética , Células Fotorreceptoras de Vertebrados/metabolismo , Príons/genética , Regiões Promotoras Genéticas , Ligação Proteica , Degeneração Retiniana/genética , Degeneração Retiniana/metabolismo , Ataxias Espinocerebelares/genética , Ataxias Espinocerebelares/metabolismo , Transmissão Sináptica , Transativadores/fisiologia , Ativação Transcricional , Transfecção , Transgenes , Técnicas do Sistema de Duplo-HíbridoRESUMO
Mutations at the Werner helicase locus (WRN) are responsible for the Werner syndrome (WS). WS patients prematurely develop an aged appearance and various age-related disorders. We have generated transgenic mice expressing human WRN with a putative dominant-negative mutation (K577M-WRN). Primary tail fibroblast cultures from K577M-WRN mice showed three characteristics of WS cells: hypersensitivity to 4-nitroquinoline-1-oxide (4NQO), reduced replicative potential, and reduced expression of the endogenous WRN protein. These data suggest that K577M-WRN mice may provide a novel mouse model for the WS.
Assuntos
DNA Helicases/genética , Genes Dominantes , Síndrome de Werner/genética , 4-Nitroquinolina-1-Óxido/análogos & derivados , 4-Nitroquinolina-1-Óxido/farmacologia , Alelos , Animais , Divisão Celular , Regulação para Baixo , Exodesoxirribonucleases , Humanos , Camundongos , Camundongos Transgênicos , Fenótipo , Quinolonas/farmacologia , RecQ Helicases , Helicase da Síndrome de WernerRESUMO
Electrolytic lesions were placed in the medial cerebellar nucleus in tree shrews (Tupaia glis) or in fibers issuing from this nucleus. Brains and spinal cords were processed according to Fink-Heimer procedure following survival times of 2-7 days. In control animals lesions were placed in the cerebellar cortex and, in one case, in the olfactory bulb. Degenerating fibers were seen entering the cervical spinal cord and continuing to thoracic and lumbar levels. The projection is relatively profuse in the cervical cord, becoming sparse as the fibers proceed to more caudal levels. Fibers run in the lateral funiculus, predominantly contralateral to the lesion. Some fibers are observed to travel directly through the intermediate gray matter of the spinal cord. Preterminal degeneration is seen primarily in the intermediate gray of the spinal cord. Results are discussed in relation to typical locomotor behavior of tree shrew.
Assuntos
Núcleos Cerebelares/anatomia & histologia , Medula Espinal/anatomia & histologia , Animais , Tronco Encefálico/anatomia & histologia , Córtex Cerebelar/anatomia & histologia , Dominância Cerebral/fisiologia , Locomoção , Degeneração Neural , Fibras Nervosas/ultraestrutura , Vias Neurais/anatomia & histologia , TupaiidaeRESUMO
Two hundred fifty-one ovine embryos were frozen in different levels of glycerol (1.0, 1.4, 2.0 or 2.8M) and thawed into one of four sucrose levels (0, 0.25, 1.0 or 2.0M) to determine the optimal glycerol-sucrose combination for one-step, in-straw thawing. Sucrose was toxic at low glycerol levels and mandatory at high levels. The 1.0M sucrose level with either 1.4 or 2.0M glycerol was optimal for one-step cryoprotectant removal.
Assuntos
Modalidades de Fisioterapia , Esquizofrenia Infantil/terapia , Pré-Escolar , Humanos , MasculinoRESUMO
Transgenic animals are a powerful tool in the exploration of the interaction of specific genes with the environment and/or toxins to understand the relation between biochemical and metabolic pathways and toxic effect. This overview discusses DNA construct design, methods for production of transgenic animals, including pronuclear fusion, gene targeting and chimera production, animal husbandry, and colony management.
Assuntos
Camundongos Transgênicos , Testes de Toxicidade/métodos , Toxicologia/métodos , Criação de Animais Domésticos , Animais , Regulação da Expressão Gênica/efeitos dos fármacos , Técnicas Genéticas , Humanos , Camundongos , Modelos Animais , Medição de RiscoRESUMO
The ability of bovine oocytes to undergo parthenogenetic activation using either a Ca++-Mg++-H+ ionophore (A23187) or electric shock was investigated, as a prelude to understanding activation potential following nuclear transfer into ooplasm. Oocytes were collected from slaughterhouse ovaries by aspiration of 1-5-mm follicles. The time of placement into maturation medium was noted, and maturational age (time in culture) measured from that point. After exposure to activating conditions eggs were cultured for a further 12-16 hours, fixed, and stained with aceto-orcein. Oocytes that progressed to telophase or pronuclear formation were considered activated. Concentrations of A23187 ranging from 100 pM to 100 microM showed that 1-100 microM levels resulted in 94-100% activation at 30 hours maturation. Frequency of activation differed from controls (no ionophore) at 100 nM (49%; P less than 0.05). With A23187 maximum response occurred between 26 and 30 hours of maturation (77% and 92%, respectively). A short pulse electric shock, capable of causing oocyte membrane fusion, gave similar results relative to maturational age (82% and 90% activation for 26 and 30 hours, respectively). Therefore, maximum response to the two activating stimuli occurred in oocytes at similar maturational ages. Exposure to activating conditions prior to onset of activating ability (18 hours) followed by another exposure at 26 hours showed that the oocytes were still fully able to activate upon reaching maturational activation competence. Because cytochalasin B is present in the medium used for nuclear transfer, oocytes were incubated with cytochalasin B prior to exposure to an activating stimulus. Frequency of activation was similar to the control treatment (61% and 73%). The effect of mechanical stress of cytoplasm removal and replacement by electrofusion on activation was also not significant. Overall, maturational age of the oocyte was the main determinant of activation ability.
Assuntos
Envelhecimento/fisiologia , Oócitos/fisiologia , Animais , Calcimicina/farmacologia , Bovinos , Estimulação Elétrica , Feminino , Oócitos/efeitos dos fármacosRESUMO
Bovine cumulus-oocyte complexes (COC) (n = 4230) were used in this study to assess the effects of culture method, hormonal supplementation, and cumulus cell concentration on maturation, fertilization and development of resulting embryos. Five treatments were evaluated. 1) 10 COC/50-microliter drops under oil in TCM 199 supplemented with 10% heat-treated fetal calf serum, follicle-stimulating hormone (0.5 microgram/ml), luteinizing hormone (5 micrograms/ml), and estradiol-17 beta (1 microgram/ml); 2) as in 1 without hormones; 3) as in 1 but in 3 ml TCM-199 in petri dishes without paraffin oil; 4) as in 2 but only 1 COC/50-microliter drop; and 5) as in 1 but with denuded oocytes. After 24 h maturation, the frequencies of oocytes reaching metaphase II were 98, 84, 92, 93, and 87%, respectively, for the five treatments. In the same order, percentages of normal fertilization were 73, 70, 62, 81, and 62%, and the frequencies of embryos containing two or more blastomeres at 65 h postinsemination were 69, 82, 66, 51, and 43%. The same five treatments were used in a second study in which 3,199 oocytes were fertilized, allowed to cleave in vitro to the 2- to 3-cell stage (42 h postinsemination), and transferred to oviducts of sheep (one treatment/oviduct) for 4 days. The frequencies of morulae or blastocytes obtained were 28, 18, 23, 24, and 11% for the five treatments, respectively. After nonsurgical transfer to bovine recipients (n = 8) using fresh or frozen-thawed embryos, three pregnancies past 50 days were obtained. Only one went to term with the birth of a live heifer calf.
Assuntos
Bovinos/fisiologia , Fertilização in vitro/veterinária , Oócitos/fisiologia , Animais , Células Cultivadas , FemininoRESUMO
Although an excitotoxic mechanism of neuronal injury has been proposed to play a role in chronic neurodegenerative disorders such as Alzheimer's disease, and neurotrophic factors have been put forward as potential therapeutic agents, direct evidence is lacking. Taking advantage of the fact that mutations in the presenilin-1 (PS1) gene are causally linked to many cases of early-onset inherited Alzheimer's disease, we generated PS1 mutant knock-in mice and directly tested the excitotoxic and neurotrophic hypotheses of Alzheimer's disease. Primary hippocampal neurons from PS1 mutant knock-in mice exhibited increased production of amyloid beta-peptide 42/43 and increased vulnerability to excitotoxicity, which occurred in a gene dosage-dependent manner. Neurons expressing mutant PS1 exhibited enhanced calcium responses to glutamate and increased oxyradical production and mitochondrial dysfunction. Pretreatment with either basic fibroblast growth factor or activity-dependent neurotrophic factor protected neurons expressing mutant PS1 against excitotoxicity. Both basic fibroblast growth factor and activity-dependent neurotrophic factor stabilized intracellular calcium levels and abrogated the increased oxyradical production and mitochondrial dysfunction otherwise caused by the PS1 mutation. Our data indicate that neurotrophic factors can interrupt excitotoxic neurodegenerative cascades promoted by PS1 mutations.
Assuntos
Doença de Alzheimer/genética , Doença de Alzheimer/prevenção & controle , Peptídeos beta-Amiloides/genética , Regulação da Expressão Gênica , Hipocampo/metabolismo , Proteínas de Membrana/genética , Neurônios/metabolismo , Mutação Puntual , Substituição de Aminoácidos , Peptídeos beta-Amiloides/biossíntese , Animais , Cálcio/metabolismo , Células Cultivadas , Cruzamentos Genéticos , Feminino , Radicais Livres/metabolismo , Ácido Glutâmico/farmacologia , Humanos , Peroxidação de Lipídeos , Masculino , Proteínas de Membrana/metabolismo , Camundongos , Camundongos Endogâmicos C57BL , Camundongos Endogâmicos , Camundongos Transgênicos , Mitocôndrias/metabolismo , Neurônios/efeitos dos fármacos , Neurotoxinas/toxicidade , Presenilina-1 , Espécies Reativas de Oxigênio/metabolismoRESUMO
Many cases of early-onset inherited Alzheimer's disease (AD) are caused by mutations in the presenilin-1 (PS1) gene. Overexpression of PS1 mutations in cultured PC12 cells increases their vulnerability to apoptosis-induced trophic factor withdrawal and oxidative insults. We now report that primary hippocampal neurons from PS1 mutant knock-in mice, which express the human PS1M146V mutation at normal levels, exhibit increased vulnerability to amyloid beta-peptide toxicity. The endangering action of mutant PS1 was associated with increased superoxide production, mitochondrial membrane depolarization, and caspase activation. The peroxynitrite-scavenging antioxidant uric acid and the caspase inhibitor benzyloxycarbonyl-Val-Ala-Asp-fluoromethyl ketone protected hippocampal neurons expressing mutant PS1 against cell death induced by amyloid beta-peptide. Increased oxidative stress may contribute to the pathogenic action of PS1 mutations, and antioxidants may counteract the adverse property of such AD-linked mutations.
Assuntos
Peptídeos beta-Amiloides/fisiologia , Caspases/metabolismo , Hipocampo/citologia , Proteínas de Membrana/metabolismo , Neurônios/metabolismo , Superóxidos/metabolismo , Doença de Alzheimer/genética , Peptídeos beta-Amiloides/toxicidade , Animais , Sobrevivência Celular/efeitos dos fármacos , Células Cultivadas , Ativação Enzimática , Hipocampo/efeitos dos fármacos , Humanos , Imuno-Histoquímica , Peroxidação de Lipídeos/efeitos dos fármacos , Peroxidação de Lipídeos/fisiologia , Proteínas de Membrana/genética , Camundongos , Camundongos Knockout , Mutação , Neurônios/efeitos dos fármacos , Presenilina-1 , Espécies Reativas de Oxigênio/metabolismoRESUMO
The differentiation of precursor cells into neurons or astrocytes in the developing brain has been thought to be regulated in part by growth factors. We show here that neural precursors isolated from the developing forebrain of mice that are deficient in the gene for the low-affinity leukemia inhibitory factor receptor (LIFR-/-) fail to generate astrocytes expressing glial fibrillary acidic protein (GFAP) when cultured in vitro. Precursors from mice heterozygous for the null allele show normal levels of GFAP expression. These findings support the in vivo findings that show extremely low levels of GFAP mRNA in brains of embryonic day 19 LIFR-/- mice. In addition, monolayers of neural cells from LIFR-/- mice are far less able to support the neuronal differentiation of normal neural precursors than are monolayers from heterozygous or wild-type animals, indicating that endogenous signaling through the LIFR is required for the expression of both functional and phenotypic markers of astrocyte differentiation. LIFR-/- precursors are not irreversibly blocked from differentiating into astrocytes: they express GFAP after long-term passaging or stimulation with bone morphogenetic protein-2. These findings strongly implicate the LIF family of cytokines in the regulation of astrocyte differentiation and indeed the LIF-deficient animals show a significant reduction in the number of GFAP cells in the hippocampus. However, because this reduction is only partial it suggests that LIF may not be the predominant endogenous ligand signaling through the LIFR.
Assuntos
Astrócitos/citologia , Inibidores do Crescimento/deficiência , Interleucina-6 , Linfocinas/deficiência , Prosencéfalo/citologia , Receptores de Citocinas/deficiência , Células-Tronco/citologia , Fator de Crescimento Transformador beta , Animais , Astrócitos/metabolismo , Proteína Morfogenética Óssea 2 , Proteínas Morfogenéticas Ósseas/farmacologia , Diferenciação Celular , Células Clonais , Células Epiteliais/citologia , Proteína Glial Fibrilar Ácida/biossíntese , Inibidores do Crescimento/genética , Heterozigoto , Hipocampo/citologia , Hipocampo/metabolismo , Homozigoto , Fator Inibidor de Leucemia , Subunidade alfa de Receptor de Fator Inibidor de Leucemia , Linfocinas/genética , Camundongos , Camundongos Endogâmicos C57BL , Camundongos Endogâmicos CBA , Camundongos Mutantes , Neurônios/citologia , Prosencéfalo/embriologia , Prosencéfalo/crescimento & desenvolvimento , RNA Mensageiro/análise , Receptores de Citocinas/genética , Receptores de OSM-LIF , Transdução de Sinais , Células-Tronco/metabolismoRESUMO
Inductive signals cause conversion of mesenchyme into epithelia during the formation of many organs. Yet a century of study has not revealed the inducing molecules. Using a standard model of induction, we found that ureteric bud cells secrete factors that convert kidney mesenchyme to epithelia that, remarkably, then form nephrons. Purification and sequencing of one such factor identified it as leukemia inhibitory factor (LIF). LIF acted on epithelial precursors that we identified by the expression of Pax2 and Wnt4. Other IL-6 type cytokines acted like LIF, and deletion of their shared receptor reduced nephron development. In situ, the ureteric bud expressed LIF, and metanephric mesenchyme expressed its receptors. The data suggest that IL-6 cytokines are candidate regulators of mesenchymal to epithelial conversion during kidney development.
Assuntos
Indução Embrionária , Epitélio/embriologia , Inibidores do Crescimento/fisiologia , Linfocinas/fisiologia , Mesoderma/fisiologia , Néfrons/embriologia , Ureter/embriologia , Animais , Antígenos CD/metabolismo , Receptor gp130 de Citocina , Proteínas de Ligação a DNA/metabolismo , Fator 2 de Crescimento de Fibroblastos/metabolismo , Inibidores do Crescimento/genética , Inibidores do Crescimento/isolamento & purificação , Interleucina-6/metabolismo , Túbulos Renais/embriologia , Fator Inibidor de Leucemia , Subunidade alfa de Receptor de Fator Inibidor de Leucemia , Ligantes , Linfocinas/genética , Linfocinas/isolamento & purificação , Glicoproteínas de Membrana/metabolismo , Camundongos , Fator de Transcrição PAX2 , Proteínas Proto-Oncogênicas/metabolismo , Ratos , Receptores de Citocinas/metabolismo , Receptores de OSM-LIF , Proteínas Recombinantes/metabolismo , Proteínas Recombinantes/farmacologia , Fator de Transcrição STAT3 , Transativadores/metabolismo , Fatores de Transcrição/metabolismo , Ureter/citologia , Proteínas Wnt , Proteína Wnt4RESUMO
The low-affinity receptor for leukemia inhibitory factor (LIFR) interacts with gp130 to induce an intracellular signal cascade. The LIFR-gp130 heterodimer is implicated in the function of diverse systems. Normal placentation is disrupted in LIFR mutant animals, which leads to poor intrauterine nutrition but allows fetuses to continue to term. Fetal bone volume is reduced greater than three-fold and the number of osteoclasts is increased six-fold, resulting in severe osteopenia of perinatal bone. Astrocyte numbers are reduced in the spinal cord and brain stem. Late gestation fetal livers contain relatively high stores of glycogen, indicating a metabolic disorder. Hematologic and primordial germ cell compartments appear normal. Pleiotropic defects in the mutant animals preclude survival beyond the day of birth.