[Vasodilatory action of FK 409 on bovine retinal arteries--analysis by a microvessel perfusion system].

PURPOSE: We investigated the vasodilatory action of FK 409 on bovine retinal arteries using a microvessel perfusion system in vitro. MATERIALS AND METHODS: Bovine retinal arteries were obtained from bovine eyes. The arteries, 3 mm long and 110 microns in diameter, were attached to the microvessel perfusion system. The arteries were perfused with Tyrode solution containing 10(-6) M U46619, followed by 10(-6) M U46619 and FK 409 (10(-3) M or 10(-4) M). We also perfused the arteries with carboxy-2-phenyl-4,4,5,5-tetramethylimidazoline-1-oxyl 3-oxide. The inner diameter of the arteries was measured under a microscope and analyzed by computer. RESULTS: FK 409 prevented contraction of bovine retinal arteries induced by perfusion of 10(-6) M U46619 when the arteries were perfused with 10(-3) to 10(-4) M FK 409. Addition of 100 microM PTIO, an nitric oxide (NO)-specific extinction agent, into the mixture of U46619 and FK 409 hadan antagonistic effect on the contractive action of FK 409. CONCLUSION: These results suggest that FK 409 has a dilatational effect on bovine retinal arteries and that the dilatational action of FK 409 is possibly produced by NO.

[Comparison of surface light scattering of acrylic intraocular lenses made by lathe-cutting and cast-molding methods--long-term observation and experimental study].

PURPOSE: To compare the long-term clinical and experimental results of soft acrylic intraocular lenses(IOLs) manufactured by the lathe-cut(LC) method and by the cast-molding(CM) method. METHODS: This was a retrospective study of 20 patients(22 eyes) who were examined in a 5- and 7-year follow-up study. Sixteen eyes were implanted with polyacrylic IOLs manufactured by the LC method and 6 eyes were implanted with polyacrylic IOLs manufactured by the CM method. Postoperative measurements included best corrected visual acuity, contrast sensitivity, biomicroscopic examination, and Scheimpflug slit-lamp images to evaluate surface light scattering. Scanning electron microscopy and three-dimensional surface analysis were conducted. RESULTS: At 7 years, the mean visual acuity was 1.08 +/- 0.24 (mean +/- standard deviation) in the LC group and 1.22 +/- 0.27 in the CM group. Surface light-seatter was 12.0 +/- 4.0 computer compatible tapes(CCT) in the LC group and 37.4 +/- 5.4 CCT in the CM group. Mean surface roughness was 0.70 +/- 0.07 nm in the LC group and 6.16 +/- 0.97 nm in the CM group. CONCLUSION: Acrylic IOLs manufactured by the LC method are more stable in long-termuse.