Your browser doesn't support javascript.
loading
Mostrar: 20 | 50 | 100
Resultados 1 - 1 de 1
Filtrar
Mais filtros

Base de dados
Ano de publicação
Tipo de documento
Intervalo de ano de publicação
1.
Elife ; 102021 07 16.
Artigo em Inglês | MEDLINE | ID: mdl-34270411

RESUMO

Extracellular electrophysiology and two-photon calcium imaging are widely used methods for measuring physiological activity with single-cell resolution across large populations of cortical neurons. While each of these two modalities has distinct advantages and disadvantages, neither provides complete, unbiased information about the underlying neural population. Here, we compare evoked responses in visual cortex recorded in awake mice under highly standardized conditions using either imaging of genetically expressed GCaMP6f or electrophysiology with silicon probes. Across all stimulus conditions tested, we observe a larger fraction of responsive neurons in electrophysiology and higher stimulus selectivity in calcium imaging, which was partially reconciled by applying a spikes-to-calcium forward model to the electrophysiology data. However, the forward model could only reconcile differences in responsiveness when restricted to neurons with low contamination and an event rate above a minimum threshold. This work established how the biases of these two modalities impact functional metrics that are fundamental for characterizing sensory-evoked responses.


Assuntos
Eletrofisiologia/métodos , Neurônios/fisiologia , Animais , Cálcio , Sinalização do Cálcio , Genótipo , Camundongos , Camundongos Transgênicos , Neurônios/citologia , Córtex Visual/citologia , Córtex Visual/fisiologia
SELEÇÃO DE REFERÊNCIAS
DETALHE DA PESQUISA