Towards a standardization of biomethane potential tests.

Production of biogas from different organic materials is a most interesting source of renewable energy. The biomethane potential (BMP) of these materials has to be determined to get insight in design parameters for anaerobic digesters. Although several norms and guidelines for BMP tests exist, inter-laboratory tests regularly show high variability of BMPs for the same substrate. A workshop was held in June 2015, in Leysin, Switzerland, with over 40 attendees from 30 laboratories around the world, to agree on common solutions to the conundrum of inconsistent BMP test results. This paper presents the consensus of the intense roundtable discussions and cross-comparison of methodologies used in respective laboratories. Compulsory elements for the validation of BMP results were defined. They include the minimal number of replicates, the request to carry out blank and positive control assays, a criterion for the test duration, details on BMP calculation, and last but not least criteria for rejection of the BMP tests. Finally, recommendations on items that strongly influence the outcome of BMP tests such as inoculum characteristics, substrate preparation, test setup, and data analysis are presented to increase the probability of obtaining validated and reproducible results.

Computational modelling of extrusion process temperatures on the interactions between black soldier fly larvae protein and corn flour starch.

Insects such as the black soldier fly (BSF) are recently being studied as food sources to address concerns about how to meet the food demand of the growing world population, as conventional production lines for meat proteins are currently unsustainable sources. Studies have been conducted evaluating the use of insect proteins to produce extruded foods such as expanded snacks and meat analogues. However, this field of study is still quite new and not much has been studied beyond digestibility and growth performance. The purpose of this work was to evaluate the compatibility of protein extracted from BSF flour with corn flour starch within an extruded balanced shrimp feed model through molecular dynamics simulations, for which cohesive energy density and solubility parameter (δ) of both components were determined. The calculations' results for the protein molecule systems yielded an average δ of 14.961 MPa0.5, while the δ for starch was calculated to be 23.166 MPa0.5. The range of difference between both δ (10 > Î´ > 7) suggests that the interaction of the BSF protein with corn starch is of a semi-miscible nature. These results suggest that it is possible to obtain a stable starch-protein mixture through the extrusion process.

Material characterization and conditioning of cattle feedlot manure as feedstock for dry batch anaerobic digestion.

The focus of the study was to determine the suitability of cattle feedlot manure originating from clay-pack feedlots as a possible feedstock material for dry batch anaerobic digestion. Oedometer tests were carried out that measure the permeability and compressibility of the feedstock under practical conditions experienced in large-scale dry batch anaerobic digestion plants. Material characterization tests showed that feedlot manure was impermeable under compression and therefore unsuitable for percolation. Mixtures of feedlot manure, wood chips (3 %ww) and wheat straw (6 %ww) showed superior permeability under compression compared to feedlot manure alone with an 56% increased permeability. Further practical tests showed that dry digestion of feedlot manure mixtures led to methane yields of 99 mL/g VS which equals 86% of the material biochemical methane potential (BMP). High percolation rate and low inoculum recycle led to the highest specific methane yield (SMY) and digester productivity with implications on process design to reduce capital investment costs.

Material Characterization and Substrate Suitability Assessment of Chicken Manure for Dry Batch Anaerobic Digestion Processes.

Chicken manure is an agricultural residue material with a high biomass potential. The energetical utilization of this feedstock via anaerobic digestion is an interesting waste treatment option. One waste treatment technology most appropriate for the treatment of stackable (non-free-flowing) dry organic waste materials is the dry batch anaerobic digestion process. The aim of this study was to evaluate the substrate suitability of chicken manure from various sources as feedstock for percolation processes. Chicken manure samples from different housing forms were investigated for their chemical and physical material properties, such as feedstock composition, permeability under compaction and material compressibility. The permeability under compaction of chicken manure ranged from impermeable to sufficiently permeable depending on the type of chicken housing, manure age and bedding material used. Porous materials, such as straw and woodchips, were successfully tested as substrate additives with the ability to enhance material mixture properties to yield superior permeability and allow sufficient percolation. In dry anaerobic batch digestion trials at lab scale, the biogas generation of chicken manure with and without any structure material addition was investigated. Digestion trials were carried out without solid inoculum addition and secondary methanization of volatile components. The specific methane yield of dry chicken manure was measured and found to be 120 to 145 mL/g volatile solids (VS) and 70 to 75 mL/g fresh matter (FM), which represents approximately 70% of the methane potential based on fresh mass of common energy crops, such as corn silage.

The Influence of Pressure-Swing Conditioning Pre-Treatment of Cattle Manure on Methane Production.

Cattle manure is an agricultural residue, which could be used as source to produce methane in order to substitute fossil fuels. Nevertheless, in practice the handling of this slowly degradable substrate during anaerobic digestion is challenging. In this study, the influence of the pre-treatment of cattle manure with pressure-swing conditioning (PSC) on the methane production was investigated. Six variants of PSC (combinations of duration 5 min, 30 min, 60 min and temperature 160 °C, 190 °C) were examined with regards to methane yield in batch tests. PSC of cattle manure showed a significant increase up to 109% in the methane yield compared to the untreated sample. Kinetic calculations proved also an enhancement of the degradation speed. One PSC-variant (190 °C/30 min) and untreated cattle manure were chosen for comparative fermentation tests in continuously stirred tank reactors (CSTR) in lab-scale with duplicates. In the continuous test a biogas production of 428 mL/g volatile solids (VS) (54.2% methane) for untreated manure was observed and of 456 mL/g VS (53.7% methane) for PSC-cattle-manure (190 °C/30 min). Significant tests were conducted for methane yields of all fermentation tests. Furthermore, other parameters such as furfural were investigated and discussed.

Biomass potential analysis of aquatic biomass and challenges for its use as a nonconventional substrate in anaerobic digestion plants.

Aquatic macrophytes are important components of aquatic habitats. However, the overgrowth of aquatic plants can cause severe problems for the management of bodies of water. As a result, these plants must be removed and disposed of as waste. However, the usage of this biomass as a substrate in biogas plants would appear to be more beneficial. The present study shows the advantages and barriers to the use of harvested aquatic biomass for energy production in Germany. The results cover several aspects of this issue, such as the question of biomass potential, the quality of the harvested aquatic plants, and ensiling for the purpose of conservation for anaerobic digestion. In addition, the social aspects of the de-weeding in bodies of water are discussed.

Effect of particle size reduction and ensiling fermentation on biogas formation and silage quality of wheat straw.

The effect of ensiling fermentation and mechanical pretreatment on the methane yield of lignocellulosic biomass was investigated in order to determine the optimum pretreatment conditions for biogas production. Wheat straw was treated using the following techniques: mechanical disintegration by chopping and extruder-grinding to particle sizes of 2.0 and 0.2cm, respectively, and ensiling by 30% and 45% total solids with addition of enzymatic, chemical and biological silage additives individually and in combination. The total and volatile solid content, biochemical methane potential and products of silage fermentation of 32 variants were tested. The results indicate that the methane potential increased by 26% (from 179 to 244mLCH4g-1VS) by reducing particle size. The maximum methane potential of 275mLCH4g-1VS was obtained from silage with 30% total solids and extruder grinding. However, the effect of the addition of silage additives on the methane potential was limited.

Disintegration in the biogas sector--technologies and effects.

Pretreatment of organic material prior to anaerobic digestion is seen as an option to increase the overall efficiency of the process. An overview of physical, chemical, and biological disintegration (DT) of substrates in the biogas sector is given. The energy demands DT were surveyed. The technologies were evaluated by reference to the Technology Readiness Assessment Guide of the U.S. Department of Energy. The evaluation focuses on ligno-cellulosic substrates like straw. Data of a survey among biogas plant operators were analyzed regarding the prevalence of disintegration technology classes in Germany. Furthermore, biochemical methane potential tests were conducted in laboratory scale to determine the specific methane yields of un-/treated barley straw (thermal pressure hydrolysis (TPH)). A methane potential of 228 ml CH4/g VS was measured for untreated barley straw; and of 251 ml CH4/g VS for TPH-straw (190 °C, 30 min). The reaction rates in BMP were calculated between 0.0976 and 0.1443 d(-1).