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The development of methods that allow a structural interpretation of linear and non-linear vibrational spectra is of great importance, both for spectroscopy and for optimizing force-field quality. The experimentally measured signals are ensemble averages over all accessible configurations, which complicates spectral calculations. To account for this, we present a recipe for calculating vibrational amide-I spectra of proteins based on metadynamics molecular dynamics simulations. For each frame, a one-exciton Hamiltonian is set up for the backbone amide groups, in which the couplings are estimated with the transition-charge coupling model for non-nearest neighbors, and with a parametrized map of ab initio calculations that give the coupling as a function of the dihedral angles for nearest neighbors. The local-mode frequency variations due to environmental factors such as hydrogen bonds are modelled by exploiting the linear relationship between the amide C-O bond length and the amide-I frequency. The spectra are subsequently calculated while taking into account the equilibrium statistical weights of the frames that are determined using a previously-published reweighting procedure. By implementing all these steps in an efficient Fortran code, the spectra can be averaged over very large amounts of structures, thereby extensively covering the phase space of proteins. Using this recipe, the spectral responses of 2.5 million frames of a metadynamics simulation of the miniprotein Trp-cage are averaged to reproduce the experimental temperature-dependent IR spectra very well. The spectral calculations provide new insight into the origin of the various spectral signatures (which are typically challenging to disentangle in the congested amide-I region), and allow for a direct structural interpretation of the experimental spectra and for validation of the molecular dynamics simulations of ensembles.
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Urea is believed to have been essential to the synthesis of prebiotic nucleotides and thereby the RNA or DNA of the first lifeforms. Models suggesting that life began in wet-dry cycles around shallow aquatic ponds imply that reactants such as urea were exposed to deep ultraviolet irradiation from the young sun. Detrimental photodissociation of urea induced by deep UV excitation potentially challenges these models. We here follow the primary deep ultraviolet photochemistry of aqueous urea. The data show that urea is barely excited at 200â nm due to weak ultraviolet absorption. The likelihood of photodissociation is further reduced by strong intra-molecular coupling of the CN and CO stretch vibrations accompanied by an efficient dissipation of the excitation energy to the surrounding water molecules mitigated by urea-water hydrogen bonds. We find that 54±5 % of the excited urea molecules dissociate. Reactions between the photoproducts and surrounding solvent molecules form carbamic acid or the carbamate anions within 0.6â ps. The molecules that do not dissociate return to the electronic ground state in 2â ps. Interestingly, the photodissociation processes of urea in the aqueous phase is different from earlier reported reactions observed following the VUV photolysis of urea in noble gas matrices and highlight the potential influence of water on the prebiotic photochemistry.
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Lysine-leucine (LK) peptides have been used as model systems and platforms for 2D material design for decades. LK peptides are amphiphilic sequences designed to bind and fold at hydrophobic surfaces through hydrophobic leucine side chains and hydrophilic lysine side chains extending into the aqueous subphase. The hydrophobic periodicity of the sequence dictates the secondary structure at the interface. This robust design makes them ideal candidates for controlling interfacial chemistry. This study presents the de novo design and characterization of two novel peptides: LRα14 and LHα14, which substitute lysine with arginine and histidine, respectively, in the helical LKα14 sequence. This modification is intended to expand the LK peptide platform to a new basic interfacial chemistry. We explore the stability of the new LRα14 and LHα14 designs with respect to changes in pH and salt concentration in bulk solution and at the interface using circular dichroism (UV-CD) and vibrational sum-frequency generation spectroscopy, respectively. Notably, the structural stability of the peptides remains unaffected across a wide range of pH and ionic strength values. At the same time, the variation of side-chain chemistry leads to a wide spectrum of interfacial water structures. By extension of the LK platform to include arginine and histidine, this study broadens the toolbox for designing tailored interfacial chemistries with applications in material and biomedical sciences.
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Diatoms, unicellular marine organisms, harness short peptide repeats of the protein silaffin to transform silicic acid into biosilica nanoparticles. This process has been a white whale for material scientists due to its potential in biomimetic applications, ranging from medical to microelectronic fields. Replicating diatom biosilicification will depend on a thorough understanding of the silaffin peptide structure during the reaction, yet existing models in the literature offer conflicting views on peptide folding during silicification. In our study, we employed two-dimensional infrared spectroscopy (2DIR) within the amide I region to determine the secondary structure of the silaffin repeat unit 5 (R5), both pre- and post-interaction with silica. The 2DIR experiments are complemented by molecular dynamics (MD) simulations of pure R5 reacting with silicate. Subsequently, theoretical 2DIR spectra calculated from these MD trajectories allowed us to compare calculated spectra with experimental data, and to determine the diverse structural poses of R5. Our findings indicate that unbound R5 predominantly forms ß-strand structures alongside various atypical secondary structures. Post-silicification, there's a noticeable shift: a decrease in ß-strands coupled with an increase in turn-type and bend-type configurations. We theorize that this structural transformation stems from silicate embedding within R5's hydrogen-bond network, prompting the peptide backbone to contract and adapt around the biosilica precursors.
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Diatomáceas , Simulação de Dinâmica Molecular , Espectrofotometria Infravermelho , Diatomáceas/química , Estrutura Secundária de Proteína , Peptídeos/química , Fragmentos de Peptídeos , Precursores de ProteínasRESUMO
The susceptibility of aqueous dipeptides to photodissociation by deep ultraviolet irradiation is studied by femtosecond spectroscopy supported by density functional theory calculations. The primary photodynamics of the aqueous dipeptides of glycyl-glycine (gly-gly), alalyl-alanine (ala-ala), and glycyl-alanine (gly-ala) show that upon photoexcitation at a wavelength of 200 nm, about 10% of the excited dipeptides dissociate by decarboxylation within 100 ps, while the rest of the dipeptides return to their native ground state. Accordingly, the vast majority of the excited dipeptides withstand the deep ultraviolet excitation. In those relatively few cases, where excitation leads to dissociation, the measurements show that deep ultraviolet irradiation breaks the Cα-C bond rather than the peptide bond. The peptide bond is thereby left intact, and the decarboxylated dipeptide moiety is open to subsequent reactions. The experiments indicate that the low photodissociation yield and in particular the resilience of the peptide bond to dissociation are due to rapid internal conversion from the excited state to the ground state, followed by efficient vibrational relaxation facilitated by intramolecular coupling among the carbonate and amide modes. Thus, the entire process of internal conversion and vibrational relaxation to thermal equilibrium on the dipeptide ground state occurs on a time scale of less than 2 ps.
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Dipeptídeos , Raios Ultravioleta , Dipeptídeos/química , Análise Espectral , Íons , AlaninaRESUMO
When a nanoparticle (NP) is introduced into a biological environment, its identity and interactions are immediately attributed to the dense layer of proteins that quickly covers the particle. The formation of this layer, dubbed the protein corona, is in general a combination of proteins interacting with the surface of the NP and a contest between other proteins for binding sites either at the surface of the NP or upon the dense layer. Despite the importance for surface engineering and drug development, the molecular mechanisms and structure behind interfacial biomolecule action have largely remained elusive. We use ultrafast sum frequency scattering (SFS) spectroscopy to determine the structure and the mode of action by which these biomolecules interact with and manipulate interfaces. The majority of work in the field of sum frequency generation has been done on flat model interfaces. This limits some important membrane properties such as membrane fluidity and dimensionalityâimportant factors in biomolecule-membrane interactions. To move toward three-dimensional (3D) nanoscopic interfaces, we utilize SFS spectroscopy to interrogate the surface of 3D lipid monolayers, which can be used as a model lipid-based nanocarrier system. In this study, we have utilized SFS spectroscopy to follow the action of lysozyme. SFS spectra in the amide I region suggest that there is lysozyme at the interface and that the lysozyme induces an increased lipid monolayer order. The binding of lysozyme with the NP is demonstrated by an increase in acyl chain order determined by the ratio of the CH3 symmetric and CH2 symmetric peak amplitudes. Furthermore, the lipid headgroup orientation s-PO2- change strongly supports lysozyme insertion into the lipid layer causing lipid disruption and reorientation. Altogether, with SFS, we have made a huge stride toward understanding the binding and structure change of proteins within the protein corona.
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Fosfolipídeos , Coroa de Proteína , Fosfolipídeos/química , Muramidase/química , Análise Espectral/métodos , Proteínas/químicaRESUMO
We study the primary photolysis dynamics of aqueous carbonate, CO32-(aq), and hydrogen carbonate, HCO3-(aq), when they are excited at λ = 200 nm. The photolysis is recorded with sub-picosecond time resolution using UV pump-Vis probe and UV pump-IR probe transient absorption spectroscopy and interpreted with the aid of density functional theory calculations. When CO32- is excited via single photon absorption at λ = 200 nm, Φ(t = 20 ps) = 82 ± 5% of the excited di-anions either detach an electron or dissociate. The electron detachment takes place from the excited state in t < 1 ps and forms ground state CO3Ë- and eaq-. Dissociation occurs from both the electronic ground and excited states of CO32-. Dissociation from the CO32- excited state is assisted by water molecules and forms CO2Ë-, OHË and OH-. The dissociation occurs both directly from the Franck-Condon region in t < 1 ps and indirectly with a time constant of τ = 13.9 ± 0.5 ps as the excited state relaxes. Dissociation of vibrationally excited CO32- molecules in the electronic ground state is also assisted by water molecules and forms CO2 and two OH- anions. The dissociation and subsequent vibrational relaxation of CO2 occur with a time constant of τ = 10.2 ± 0.5 ps. The residual 1 - Φ(t = 20 ps) = 18 ± 5% of the excited CO32- di-anions return by internal conversion to the equilibrated CO32- ground state with a time constant of τ = 4.0 ± 0.4 ps. The extinction coefficient of aqueous hydrogen carbonate HCO3-(aq) at λ = 200 nm is an order of magnitude smaller than that of carbonate, so even though the hydrogen carbonate anions dominate the carbonate di-anions in the hydrogen carbonate solution, the primary photolysis of hydrogen carbonate is obscured by the photo-products of carbonate. Hence, we are unable to assess the primary photolysis of hydrogen carbonate. However, the weak one-photon absorption facilitates two-photon ionization of water, which forms hydronium, H3O+, cations. The sudden increase in the acidity induced by two-photon ionization protonates the ground state hydrogen carbonate molecules, thus offering a rare spectroscopic glimpse of aqueous carbonic acid.
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Two-dimensional (2D) materials have attracted attention for potential applications in light harvesting, catalysis, and molecular electronics. Mineral proteins involved in hard tissue biogenesis can produce 2D structures with high fidelity by using sustainable production routes. This study shows that a peptide mimic based on the catalytic triad of the marine sponge protein silicatein catalyzes the formation of nanometer thin and stable sheets of silicon dioxide and titanium dioxide.
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Poríferos , Dióxido de Silício , Animais , Catepsinas/química , Catepsinas/metabolismo , Peptídeos/metabolismo , Dióxido de Silício/química , TitânioRESUMO
The mechanical properties of biomaterials are dictated by the interactions and conformations of their building blocks, typically proteins. Although the macroscopic behavior of biomaterials is widely studied, our understanding of the underlying molecular properties is generally limited. Among the noninvasive and label-free methods to investigate molecular structures, infrared spectroscopy is one of the most commonly used tools because the absorption bands of amide groups strongly depend on protein secondary structure. However, spectral congestion usually complicates the analysis of the amide spectrum. Here, we apply polarized two-dimensional (2D) infrared spectroscopy (IR) to directly identify the protein secondary structures in native silk films cast from Bombyx mori silk feedstock. Without any additional peak fitting, we find that the initial effect of hydration is an increase of the random coil content at the expense of the helical content, while the ß-sheet content is unchanged and only increases at a later stage. This paper demonstrates that 2D-IR can be a valuable tool for characterizing biomaterials.
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Bombyx , Fibroínas , Animais , Seda/química , Bombyx/química , Fibroínas/química , Espectrofotometria Infravermelho , Materiais Biocompatíveis , Amidas , Espectroscopia de Infravermelho com Transformada de FourierRESUMO
Ice active bacteria can catalyze water freezing at high subzero temperatures using ice nucleating proteins (INPs) located at their outer cell walls. INPs are the most effective ice nucleators known and are of significant interest for agriculture, climate research, and freeze/antifreeze technologies. The aggregation of INPs into large ice nucleation sites is a key step for effective ice nucleation. It has been proposed that ice active bacteria can drive the aggregation of INPs and thereby trigger ice nucleation. However, the mechanism of INP aggregate assembly and the molecular processes behind the activation are still unclear. Both biochemical pathways and activation through electrostatics have been proposed based on experiments with lysed ice active bacteria. For a more direct view on the assembly of INPs, we follow the structure and water interactions of a synthetic model INP of the well-studied ice bacterium Pseudomonas syringae at the air-water interface as a function of the subphase pH. By combining sum frequency generation spectroscopy with two-dimensional infrared spectra, we conclude that self-assembly and electrostatic interactions drive the formation of ordered INP structures capable of aligning interfacial water.
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Proteínas da Membrana Bacteriana Externa , Gelo , Bactérias/metabolismo , Proteínas da Membrana Bacteriana Externa/química , Proteínas da Membrana Bacteriana Externa/metabolismo , Congelamento , Eletricidade Estática , Água/químicaRESUMO
The fascinating adhesion of gecko to virtually any material has been related to surface interactions of myriads of spatula at the tips of gecko feet. Surprisingly, the molecular details of the surface chemistry of gecko adhesion are still largely unknown. Lipids have been identified within gecko adhesive pads. However, the location of the lipids, the extent to which spatula are coated with lipids, and how the lipids are structured are still open questions. Lipids can modulate adhesion properties and surface hydrophobicity and may play an important role in adhesion. We have therefore studied the molecular structure of lipids at spatula surfaces using near-edge X-ray absorption fine structure imaging. We provide evidence that a nanometre-thin layer of lipids is present at the spatula surfaces of the tokay gecko (Gekko gecko) and that the lipids form ordered, densely packed layers. Such dense, thin lipid layers can effectively protect the spatula proteins from dehydration by forming a barrier against water evaporation. Lipids can also render surfaces hydrophobic and thereby support the gecko adhesive system by enhancement of hydrophobic-hydrophobic interactions with surfaces.
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Lagartos , Sensilas , Adesividade , Animais , Metabolismo dos Lipídeos , Lipídeos/química , Lagartos/metabolismo , Proteínas , Sensilas/metabolismoRESUMO
Proteins at interfaces play important roles in cell biology, immunology, bioengineering, and biomimetic material design. Many biological processes are based on interfacial protein action, ranging from cellular communication to immune responses and the protein-driven mineralization of bone. Despite the importance of interfacial proteins, comparatively little is known about their structure. The standard methods for studying crystalline or solution-phase proteins (X-ray diffraction and NMR spectroscopy) are not well-suited for studying proteins at interfaces, and for these proteins we still lack a corresponding technique that can provide the same level of structural resolution. This is not surprising in view of the challenges involved in probing the structure of proteins within monomolecular films assembled at a very thin interface in situ. Vibrational sum-frequency generation (SFG) spectroscopy has the potential to overcome this challenge and investigate the structure and dynamics of proteins at interfaces at the molecular level with subpicosecond time resolution. While SFG studies were initially limited to simple model peptides, the past decade has seen a dramatic advancement of experimental techniques and data analysis methods that has made it possible to also study interfacial proteins and their folding, binding, orientation, hydration, and dynamics. In this review, we first explain the principles of SFG spectroscopy and the experimental and theoretical methods to measure and analyze protein SFG spectra. Then we give an extensive overview of the interfacial proteins studied to date with SFG. We highlight representative examples to demonstrate recent advances in probing the structure of proteins at the interfaces of liquids, membranes, minerals, and synthetic materials.
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Peptídeos/química , Proteínas/química , Análise Espectral/métodos , Animais , Humanos , Análise Espectral/instrumentação , VibraçãoRESUMO
We study the primary dissociation dynamics of aqueous formamide (HCONH2) and dimethylformamide (HCON(CH3)2) induced by photo-excitation at λ = 200 nm. The photolysis is recorded with sub-picosecond time resolution by UV pump-IR probe transient absorption spectroscopy. Formamide dissociates with a quantum yield of Φ(t = 20 ps) = 0.30 ± 0.05, t = 20 ps after the excitation. The rest of the excited formamide molecules return to the ground state within t = 1 ps and vibrationally relax towards equilibrium in t ≈ 10 ps. The only product observed is NH3. NH3 is produced with a yield of Φ(NH3) = 0.23 ± 0.10 on a timescale of τ = 3 ± 1 ps and likely constitutes the dominating product. The CO counter product to NH3 is not observed. Dimethylformamide is photolysed with a quantum yield of Φ(t = 30 ps) = 0.29 ± 0.05, t = 30 ps after the excitation. The photolysis of dimethylformamide produces CO on a time scale of τ ≈ 30 ps. The data indicate that dimethylamine and the N(CH3)2 radical are likely photoproducts.
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Dimetilformamida , Água , Fotólise , Formamidas , DimetilaminasRESUMO
We study the primary photolysis dynamics of lactic acid induced by excitation at λ = 200 nm with the aim of elucidating how simple aqueous carboxyl acids react to the deep ultraviolet exposure on the prebiotic Earth. UV-IR transient absorption spectroscopy shows a photolysis quantum yield of Φ(100 ps) = 100 ± 5%. The primary products are CO2, CO2Ë- and their counter products CH3CHOHË and CH3CHOH-. DFT calculations suggest that the dissociation takes place from the strongly acidic nπ* excited state. Dehydroxylation of lactic acid is not observed.
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Dióxido de Carbono , Ácido Láctico , Descarboxilação , Fotólise , Análise EspectralRESUMO
We used two-dimensional infrared spectroscopy to disentangle the broad infrared band in the amide II vibrational regions of Bombyx mori native silk films, identifying the single amide II modes and correlating them to specific secondary structure. Amide I and amide II modes have a strong vibrational coupling, which manifests as cross-peaks in 2D infrared spectra with frequencies determined by both the amide I and amide II frequencies of the same secondary structure. By cross referencing with well-known amide I assignments, we determined that the amide II (N-H) absorbs at around 1552 and at 1530 cm-1 for helical and ß-sheet structures, respectively. We also observed a peak at 1517 cm-1 that could not be easily assigned to an amide II mode, and instead we tentatively assigned it to a Tyrosine sidechain. These results stand in contrast with previous findings from linear infrared spectroscopy, highlighting the ability of multidimensional spectroscopy for untangling convoluted spectra, and suggesting the need for caution when assigning silk amide II spectra.
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Bombyx , Amidas/química , Animais , Seda , Espectrofotometria Infravermelho/métodos , Tirosina , VibraçãoRESUMO
A protein-based lubricating substance is discovered in the femoro-tibial joint of the darkling beetle Zophobas morio (Insecta). The substance extrudes to the contacting areas within the joint and appears in a form of filiform flows and short cylindrical fragments. The extruded lubricating substance effectively reduces the coefficient of sliding friction to the value of 0.13 in the tribosystem glass/lubricant/glass. This value is significantly lower than 0.35 in the control tribosystem glass/glass and comparable to the value of 0.14 for the tribosystem glass/dry PTFE (polytetrafluoroethylene or Teflon). The study shows for the first time that the friction-reducing mechanism found in Z. morio femoro-tibial joints is based on the lubricant spreading over the contacting surfaces rolling or moving at low loads and deforming at higher loads, preventing direct contact of joint counterparts. Besides Z. morio, the lubricant has been found in the leg joints of the Argentinian wood roach Blaptica dubia.
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Insetos , Lubrificantes , Animais , Fricção , LubrificaçãoRESUMO
Petroleum asphaltenes are surface-active compounds found in crude oils, and their interactions with surfaces and interfaces have huge implications for many facets of reservoir exploitation, including production, transportation, and oil-water separation. The asphaltene fraction in oil, found in the highest boiling-point range, is composed of many different molecules that vary in size, functionality, and polarity. Studies done on asphaltene fractions have suggested that they interact via polyaromatic and heteroaromatic ring structures and functional groups containing nitrogen, sulfur, and oxygen. However, isolating a single pure chemical structure of asphaltene in abundance is challenging and often not possible, which impairs the molecular-level study of asphaltenes of various architectures on surfaces. Thus, to further the molecular fundamental understanding, we chose to use functionalized model asphaltenes (AcChol-Th, AcChol-Ph, and 1,6-DiEtPy[Bu-Carb]) and model self-assembled monolayer (SAM) surfaces with precisely known chemical structures, whereby the hydrophobicity of the model surface is controlled. We applied solutions of asphaltenes to these SAM surfaces and then analyzed them with surface-sensitive techniques of near-edge X-ray absorption fine structure (NEXAFS) and X-ray photoelectron spectroscopy (XPS). We observe no adsorption of asphaltenes to the hydrophobic surface. On the hydrophilic surface, AcChol-Ph penetrates into the SAM with a preferential orientation parallel to the surface; AcChol-Th adsorbs in a similar manner, and 1,6-DiEtPy[Bu-Carb] binds the surface with a bent binding geometry. Overall, this study demonstrates the need for studying pure and fractionated asphaltenes at the molecular level, as even within a family of asphaltene congeners, very different surface interactions can occur.
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We study the primary reaction dynamics of aqueous oxalate following photo-excitation of the nO â πCO* transition at λ = 200 nm. After the excitation, some of the oxalate molecules return to the electronic ground state on two very different time scales: a fast component of τ = 1.1 ± 0.5 ps comprising 40% of the excited molecules and a much slower component of τ = 0.28 ± 0.05 ns accounting for 15% of the excited molecules. The remaining 45% of the excited molecules do not return to the ground state during the first 500 ps, because they either detach an electron, dissociate or stay excited for hundreds of picoseconds. Dissociation and electron detachment of oxalate predominantly produces CO2 molecules with only minor yields of CO2Ë- radical anions. The CO2 formation is accompanied by the ejection of electrons.
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We study the primary photolysis dynamics of aqueous lactate induced by photo-excitation at λ = 200 nm. Our calculations indicate that both decarboxylation and dehydroxylation are energetically possible, but decarboxylation is favoured dynamically. UV pump - IR probe transient absorption spectroscopy shows that the photolysis is dominated by decarboxylation, whereas dehydroxylation is not observed. Analysis of the transient IR spectrum suggests that photo-dissociation of lactate primarily produces CO2 and CH3CHOH- through the lowest singlet excited state of lactate, which has a lifetime of τ = 11 ps. UV pump - VIS probe transient absorption spectroscopy of electrons from the dissociating lactate anion indicates that the anionic electron from the CO2Ë- fragment is transferred to the CH3CHOHË counter radical during the decarboxylation process, and CO2Ë- is consequently only observed as a minor photo-product. The photo-dissociation quantum yield after the full decay of the excited state is Φ(100ps) = 38 ± 5%.
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Previous studies indicate that 2D materials such as graphene, WS2, and MoS2 deposited on oxidized silicon substrate are susceptible to aging due to the adsorption of airborne contamination. As a result, their surfaces become more hydrophobic. However, it is not clear how ubiquitous such a hydrophobization is, and the interplay between the specific adsorbed species and resultant wetting aging remains elusive. Here, we report a pronounced and general hydrophilic-to-hydrophobic wetting aging on 2D InSe films, which is independent of the substrates to synthesize these films (silicon, glass, nickel, copper, aluminum oxide), though the extent of wetting aging is sensitive to the layer of films. Our findings are ascribed to the occurrence and enrichment of airborne contamination that contains alkyl chains. Our results also suggest that the wetting aging effect might be universal to a wide range of 2D materials.