RESUMO
Complementary DNA segments that encode different domains of human and rat androgen receptors were fused to the Escherichia coli trpE gene using pATH expression vectors. Fusion proteins expressed by the bacteria were used to immunize rats and rabbits to obtain polyclonal antibodies to androgen receptors. Spleen cells of immunized rats were fused with myeloma cells to obtain stable hybridomas that produced monoclonal antibodies. Gradient centrifugation and immuno-precipitation assays indicated that the antibodies interacted with androgen receptors specifically.
Assuntos
Anticorpos Monoclonais/imunologia , Receptores Androgênicos/análise , Proteínas Recombinantes de Fusão/análise , Proteínas Recombinantes/análise , Sequência de Aminoácidos , Animais , Centrifugação com Gradiente de Concentração , Eletroforese em Gel de Poliacrilamida , Escherichia coli , Vetores Genéticos , Humanos , Hibridomas/imunologia , Dados de Sequência Molecular , Plasmídeos , Testes de Precipitina , Coelhos , Ratos , Receptores Androgênicos/imunologiaRESUMO
Unemployment is the most significant influence on the levels of psychological distress of young adults. Unlike the situation for the adult population, social class and income are not contributory factors. Social class of origin, however, does have a contributory effect. Feelings of lack of control and attribution of responsibility for employment solely to structural or political factors increase the impact of unemployment. Evidence in relation to employment commitment does not support "culture of poverty" type explanations. Unemployed youth appear to be "people with a problem" rather than "problem people".