RESUMO
Orthopantomography (OPG) is a routine imaging method in dental practice and an essential di- agnostic tool in dentistry. However, OPGs are challenging to interpret due to many overlapping structures. Graduates of dental schools should be aware of image distortions caused by various factors and be able to distinguish them from typical structures to make an accurate diagnosis. The aim was to determine the correlation between the knowledge regarding the location of craniofacial structures of the 1st through 3rd- year dental students and the ability to recognized them on OPGs. The study was conducted in 2021 on 131 dental students using the Microsoft Teams program. Each participant had to determine the location of 4 anthropometric points on 4 OPGs. Using proprietary software, the authors determined the Articular angle between them. The researchers performed the statistical analysis. The Bioethics Committee approved the research. The results of students from particular years of studies did not show statistical significance. There was no statistically significant difference between males and females. Only the answers from third-year male students regarding the one Articular angle showed statistically significant differences compared to the rest of the participants. Recognizing joint structures on OPG is very important from the clinical point of view, although it is often overlooked in diagnostics. Students in the first years of dental school acquire the ability to assess and analyze TMJ on OPGs properly. OPGs have many advantages, including high availability, low cost and low radiation dose. In conclusion we can say that in the first three years of studies, dental students' image assessment skills in the craniofacial radiology field remain the same.
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COVID-19 , Radiografia Panorâmica , Estudantes de Odontologia , Humanos , Masculino , Feminino , COVID-19/diagnóstico por imagem , Radiografia Panorâmica/métodos , SARS-CoV-2 , Competência Clínica , Pandemias , Adulto , Educação em Odontologia/métodosRESUMO
Chronic pain in the head and face region has a predicted prevalence of 20% in Europe, and is more common in women than men. The etiology of temporomandibular disorder (TMD) is multifactorial, and high levels of psychological stress amplify its symptoms. We were interested in how headache reported in RDC/TMD is associated with stress. Individuals with an average age of 18 years were included in the study. They were all volunteer participants in a research project. Clinical evaluation of each subject was performed using the RDC/TMD dual-axis diagnostic system. All participants filled out the PSS-10 questionnaire. A total of 138 individuals participated in the study, of which 107 were female. Headache was reported by 83 participants (59.4%), with females presenting higher scores on the PSS-10; this was statistically significant. A comparison between PSS-10 questionnaire results and headache level shows insignificant differences. However, the higher the stress level in the participant, the higher the headache score. Females are more susceptible to perceived stress, which can have an effect on TMD.
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Transtornos da Articulação Temporomandibular , Masculino , Feminino , Adulto Jovem , Humanos , Adolescente , Transtornos da Articulação Temporomandibular/complicações , Transtornos da Articulação Temporomandibular/diagnóstico , Transtornos da Articulação Temporomandibular/epidemiologia , Cefaleia/etiologia , Cefaleia/complicações , Inquéritos e Questionários , Estresse Psicológico/complicações , PrevalênciaRESUMO
Face proportions are under constant evaluation during prosthetic or surgical diagnostics and treatment. The aim was to evaluate the proportions of the faces of young Polish women as compared with other ethnic groups. MATERIALS AND METHODS: The evaluation included 60 photographs of the faces of women, average age 17.9 years. En face images were made with the camera lens situated at the same distance from the photographed subjects. Using Adobe Photoshop software, midsaggital and even anthropometric points were determined in the photographs and then the distance between these points was measured. Four relationships between vertical measurements, 3 between horizontal measurements, and 4 proportions between vertical and horizontal measurements were calculated. The results were evaluated in comparison with the golden ratio (1.618) and the measurements performed for native Americans and Latin American beauty contests winners described in the relevant literature. RESULTS: The result for the average vertical face proportion value of the examined women was close to the golden ratio (GR) and amounted to 1.592 for the proportions between nasion-stomion and stomion-gnation (98% of GR) and 1.372 for nasion-subnasale and subnasale-gnation (85% of GR).Average measurement results for young Polish women's faces were not statistically significantly different for 6 out of 11 relationships under evaluation as compared with the population of native American females and only 2 face proportions were the same as for beauty contests winners from Latin America. CONCLUSION: Face proportions of young Polish women were different from those of the females living in the American continent.
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Antropometria/métodos , Face/anatomia & histologia , Adolescente , Beleza , Feminino , Humanos , Polônia/etnologia , Adulto JovemRESUMO
PURPOSE: The prevalence of temporomandibular disorders (TMD) in the general Polish population has not yet been thoroughly investigated. The aim of this study was to determine how frequently Polish young adults demonstrated the signs and symptoms of TMD, and how often they were aware of these. MATERIALS AND METHODS: The study involved 260 volunteers of approximately 18 years of age (and with an F:M ratio of 2.8), who had completed the Research Diagnostic Criteria for Temporomandibular Disorders (RDC/TMD) questionnaire in its internationally accepted Polish version. The volunteers were students from three randomly selected high schools in Kraków, Poland. Following the clinical examinations, all participants received none or one or more of the RDC/TMD axis I diagnoses on one or both sides: muscle disorders (group I), disk displacements (group II), arthralgia, osteoarthritis, and osteoarthrosis (group III). Classification on RDC/TMD axis II involved pain-related impairment (Graded Chronic Pain Severity, from 0: no TMD pain in the prior 6 months, to IV: high disability-severely limiting), depression, and somatization levels (normal, moderate, severe). RESULTS: On the RDC/TMD axis I, 69 (26.5%) of the participants received one or more of the possible diagnoses. Awareness of TMD was present in 20 (7.7%) people from that group, in the form of self-complaints. The difference between females and males was statistically significant (p = 0.0013). On the RDC/TMD axis II assessment, a diagnosis of pain, depression, or somatization was given in 38 (14.6%) cases from that group. CONCLUSION: Muscle disorders were most frequently diagnosed in the group of Polish young adults, and the prevalence of the disease did not differ across similar groups of young people.
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Dor Facial/epidemiologia , Transtornos da Articulação Temporomandibular/epidemiologia , Adolescente , Avaliação da Deficiência , Dor Facial/etiologia , Feminino , Humanos , Masculino , Medição da Dor , Polônia/epidemiologia , Prevalência , Inquéritos e Questionários , Transtornos da Articulação Temporomandibular/diagnóstico , Adulto JovemRESUMO
BACKGROUND: Symmetry evaluation of the craniofacial complex generally involves models of mandibular movement and masticatory muscle activity, especially during the development of the craniofacial complex. The aim of this screening study was to detect differences in the asymmetry and activity indices and in the occlusal contact distribution in groups with and without orthodontic treatment, and between the sexes in the healthy population. MATERIAL AND METHOD: This screening study involved the participation of 149 Caucasian (F = 101, M = 48) 18-year-old volunteers, of whom 77 had received orthodontic treatment (Group I) and 72 had not (Group II). All participants underwent sEMG recording with an eight-channel electromyograph (Bio EMG III). A T-Scan III device was used to analyze the occlusal contact points. We measured the voltage of the right and left temporalis anterior (RTA, LTA) and of the right and left masseter muscles (RMM, LMM). On the basis of the Naeije study, we calculated the Asymmetry and Activity indices (AsI, AcI). RESULTS: No significant differences were found in the asymmetry or activity indices, or in the occlusal contact distribution of young adult subjects with or without orthodontic treatment. There were two findings in the females. First (p = 0.04), a higher voltage (131.12 µV) was recorded in the right temporalis anterior muscle in female group, compared to 119.65 µV in the male group. Secondly (p = 0.002), the activity index showed a predominance of the temporalis anterior (AcI = 10.52). In the males, the activity index showed a predominance of the masseter muscles (AcI =-1.22). CONCLUSIONS: The null hypothesis was supported, as we found that no significant differences were observed in occlusal contact, asymmetry, or activity indices between healthy young adults with or without orthodontic treatment. However, there do exist significant differences in the activity index between genders.
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Músculo Masseter , Músculos da Mastigação , Aparelhos Ortodônticos , Músculo Temporal , Adolescente , Estudos de Casos e Controles , Feminino , Humanos , Masculino , Músculo Masseter/fisiologia , Músculos da Mastigação/fisiologia , Músculo Temporal/fisiologia , Adulto JovemRESUMO
OBJECTIVES: The aim of this study was to assess the relationship between the morphology of dental arches and the activity of the masticatory muscles activities in healthy volunteers with full natural dentition. METHODS: Two-hundred youthful Class I volunteers (113 females, 87 males) were clinically investigated. Alginate impressions of dental arches were taken, and plaster casts were prepared and measured. EMG data from eight masticatory muscles was recorded to assess their activities in central occlusion, lateral and protrusive movements. RESULTS: Clinical measurements and plaster casts analyses confirmed normal values of parameters investigated. Most of the arch measurements were significantly larger in the males than in the females. Weak positive correlations were found between overbite and masseter activity in centric occlusion (the right Mm Râ=â0.151, P ≤ 0.05; the left Mm Râ=â0.191, P ≤ 0.05). Also, the range of protrusive movement positively correlated with masseter activities in central occlusion (the right Mm Râ=â0.194, P ≤ 0.05; the left Râ=â0.201, P ≤ 0.05). CONCLUSIONS: The null hypothesis that morphology of dental arches does not affect the masticatory muscles' activities was rejected. The findings of this investigation indicate that systemic, longitudinal analyses of morphology of occlusion and muscular response, even in normal subjects, are needed.
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Arco Dental/anatomia & histologia , Oclusão Dentária , Músculos da Mastigação/fisiologia , Adolescente , Eletromiografia , Feminino , Voluntários Saudáveis , Humanos , Masculino , Modelos Dentários , Adulto JovemRESUMO
Objectives: This study aimed to find if a relationship exists between antero-posterior malocclusions and the level of musculoskeletal disorders in adults, including body posture and static foot analysis. Methods: In all, 420 participants were recruited through convenience sampling (Kraków University students and patients of a local dentist's practice). Following general medical interviews, dental examinations and consideration of inclusion and exclusion criteria, 90 healthy volunteers (ages 19-35) were enrolled and assigned to three groups (n = 30) based on occlusion type (Angle's molar Class I, II or III). The research procedure involved occlusion and temporomandibular disorder assessment conducted by a dental specialist. Comprehensive morphological measurements of body asymmetry were performed using the Videography 2D package and FreeSTEP software, which calculated the parameters determined from anterior, posterior and lateral projection photos. Foot loading distribution was analyzed using the FreeMED baropodometric platform. Results: Significant differences were demonstrated in the positioning of the head, cervical and lumbar spine in the sagittal plane among individuals with the analyzed occlusal classes (p < 0.05). Individuals with Angle's Class II exhibited significantly greater forward head positions and greater depths of cervical and lumbar lordosis compared with individuals with Class III or Class I. Those with overbites had higher forefoot loading. The Class III individuals exhibited greater L-R displacement, indicating a larger angle of displacement of the centers of the right and left feet relative to the lower edge of the measurement platform, suggesting pelvic rotation. Conclusions: An inclination for concurrent occurrences of malocclusions and posture deviations in the sagittal plane was observed. An interdisciplinary approach involving dentistry and physiotherapy specialists which utilizes tools for comprehensive posture assessment is crucial for diagnosing and treating such conditions.
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BACKGROUND: Approximately half of the adult population in Europe have used some form of dental prosthesis. Much effort has been put into developing denture cleaning methods and the most recommended are brushing the prosthesis after meals and cleaning it with special liquids (sometimes prepared just before the procedure). However, these simple techniques are often omitted or insufficient due to, i.e., age-related mental or motor disabilities. OBJECTIVES: The aim of the study was to compare a range of techniques that can be performed at home and do not require patient dexterity in order to find the most efficient method of reducing the viability of the C. albicans biofilm and removing it from acrylic surfaces. MATERIAL AND METHODS: The 20 mm × 25 mm × 1 mm unpolished acrylic plates were inoculated with C. albicans and incubated for 72 h. Plates with formed biofilms were divided into 6 equal groups: a control group and 5 groups for different cleaning procedures: a dental cleaner with liquid, a dental cleaner with phosphate-buffered saline (PBS), air drying, antiseptic liquid, and an ultrasonic cleaner. Biofilm viability was assessed by plating serial dilutions and counting the colonies of C. albicans on the Sabouraud dextrose agar (SDA) medium. RESULTS: The study found that both MultiClean fluid and Sonic-3 ultrasonic cleaner were effective against Candida cells. MultiClean fluid showed the strongest biocidal properties, both when used with the Sonic Denture Cleaner and independently. CONCLUSIONS: Cleaning acrylic surfaces with a dental cleaner followed by antiseptic liquid is more effective than using these methods separately.
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Resinas Acrílicas , Anti-Infecciosos Locais , Humanos , Candida albicans , Bases de Dentadura , BiofilmesRESUMO
Background: There is still an ongoing debate about the role of the craniomandibular system, including occlusal conditions, on postural stability. This study aims to assess the role of antero-posterior malocclusion on postural control and plantar pressure distribution during standing and walking. Methods: 90 healthy volunteers (aged 19 to 35) were qualified for the study. The subjects were assigned to three groups, depending on the occlusion type. Each group (Angle Class I, II and III) consisted of 30 people. The research procedure included a clinical occlusal assessment performed by a dentist. Postural control measurements were carried out using a force platform by measuring plantar pressure distribution during standing (six trials with and without visual control) and walking test conditions. Results: The tendency to shift the CoP forward is demonstrated by Angle Class II subjects and backwards by Class I and III subjects (p < 0.001). Individuals with a malocclusion demonstrated significantly higher selected stabilographic parameters while standing on both feet (with eyes open and closed) and during the single-leg test with eyes open (p < 0.05). The analysis of the dynamic test results showed no significant correlations between Angle Classes and the selected gait parameters. Conclusions: Analyses conducted among individuals with malocclusions showed the impact of occlusion on static postural stability. In order to diagnose and effectively treat malocclusion, a multidisciplinary approach with the participation of dentists and physiotherapy specialists is necessary, with the use of stabilometric and kinematic posture assessments.
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Oclusão Dentária , Má Oclusão , Humanos , Adulto , Marcha , Postura , Posição Ortostática , Equilíbrio PosturalRESUMO
Temporomandibular joint dysfunction (TMD) is not a single diagnosis, but a term covering a group of conditions that involve pain and dysfunction of the masticatory muscles within the temporomandibular joint (TMJ) and associated structures. It is a set of disease entities comprising various ailments and clinical symptoms. One of the most distressing symptoms for TMD patients is pain. Pain is subjective and always unpleasant. The VAS (visual analogue scale) was used in this research. The aim of this study was to assess the influence of physical stimuli, namely extremely low frequency magnetic field (ELF-MF) and LED light, on the experience of pain caused by increased tension in the masticatory muscles in adults. Out of 150 people examined, 104 were enrolled in the study after meeting the eligibility criteria. The study group was divided into 4 subgroups. Each subgroup received physical therapy treatment using a different physical stimulus. The effects of four therapeutic modalities were compared. In terms of VAS scores, pain attenuation was observed in all subgroups. The study confirmed the analgesic effect of the selected physical therapy methods. The authors focused on the analysis of the results obtained for each subgroup, comparing the effects of individual modalities on pain intensity (according to VAS scores). After the treatment, pain relief was observed in each of the studied subgroups. Treatment using ELF-MF and ELF-MF in combination with LED light in the course of TMD brings about a significant improvement in the subjective pain experience expressed in VAS pain scores. The use of selected physical stimuli and their beneficial effect on pain symptoms during mandibular movements has important implications for patients' daily life and work. Incorporation of therapeutic methods can help enhance patient satisfaction and comfort during manual TMJ therapy and lengthy dental procedures.
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Transtornos da Articulação Temporomandibular , Humanos , Adulto , Resultado do Tratamento , Transtornos da Articulação Temporomandibular/complicações , Transtornos da Articulação Temporomandibular/terapia , Transtornos da Articulação Temporomandibular/diagnóstico , Articulação Temporomandibular , Dor , AnalgésicosRESUMO
OBJECTIVE: It is believed that oral infections can increase the risk of systematic diseases, such as atherosclerosis and coronary heart disease, stroke, chronic obstructive pulmonary disease, diabetes, cancer, rheumatoid arthritis, etc. It seems that oral invasive pathogens induce a systemic inflammatory response via mediators released by the cardiovascular system and liver, which increases the risk to the patient of these systematic infections, such as hypertension. On the basis of previous studies of the stomatognathic system, investigating the coexistence of systemic diseases and inflammation in the oral cavity, it can be expected that there is a connection between inflammation of the denture-bearing area in patients using acrylic removable dentures and the presence of systemic diseases, and that patients with inflammation in oral mucosa are more likely to have systemic diseases. MATERIAL AND METHOD: A retrospective study was carried out on a group of patients seeking prosthetic treatment at the Prosthetic Department of the University Dental Clinic (UKS) from March 2012 to February 2013. All data were collected using a UKS electronic database with KS-SOMED. The minimum period of use for removable prostheses was five years. RESULTS: According to anamnesis, the most common systemic diseases in our study group were hypertension disease. In total, 58% of patients with hypertension disease had no inflammation in the oral cavity. CONCLUSIONS: The occurrence of systemic diseases in edentulous people using removable prosthetic restorations, and the subsequent use of medications for these diseases, may result in a lack of clinical symptoms of concomitant fungal infection of the oral mucosa.
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Prótese Dentária , Inflamação , Doenças da Boca , Micoses , Humanos , Doenças da Boca/complicações , Doenças da Boca/imunologia , Mucosa Bucal , Boca Edêntula , Estudos RetrospectivosRESUMO
OBJECTIVES: To answer to the following questions: is there any relationship between oral hygiene and the growth of yeast in patients without mucosal inflammation; and is there a need for mycological examination patients without mucosal inflammation? BACKGROUND: Patients with candidiasis may report varied symptoms, but such infections are most often asymptomatic. In addition to its high incidence in denture users (60%-100%), there is a concern that Candida species from the oral cavity may colonize the upper gastrointestinal tract and lead to septicemia, which has a 40%-79% mortality rate and can require a prolonged hospital stay. It is thus important for all physicians to be aware of the risk factors, diagnosis, and treatment of oral candidiasis in older patients. METHODS: A retrospective study was carried out on a group of patients who had undergone mycological examination and assessment of the intensity of yeast growth, and oral hygiene. RESULTS: Ninety-one denture wearers who lacked signs of clinical inflammation were included in the study. The growth of Candida albicans was as follows: 14 patients had up to 20 colonies; 19 patients had over 20 colonies. Ten percent of patients with good oral hygiene proved to have more than 20 yeast colonies. 5% of patients with bad oral hygiene had more than 20 colonies. CONCLUSIONS: There was no relationship found between hygiene and the growth rate of fungal microorganisms. In patients without clinical symptoms of stomatitis, mycological examination should be considered.
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Candidíase Bucal/epidemiologia , Dentaduras/microbiologia , Higiene Bucal/estatística & dados numéricos , Adulto , Idoso , Idoso de 80 Anos ou mais , Candidíase Bucal/diagnóstico , Feminino , Humanos , Incidência , Masculino , Pessoa de Meia-Idade , Estudos Retrospectivos , Fatores de RiscoRESUMO
BACKGROUND AND PURPOSE: Temporomandibular disorders (TMD) are among the most frequent pathologies of the stomatognathic system. One problem often associated with TMD is the psychoemotional status. The aim of study was to evaluate the psychoemotional status of young adults with pain symptoms associated with TMD. MATERIAL AND METHODS: We analyzed the data of 260 volunteers. The Research Diagnostic Criteria for Temporomandibular Disorders (RDC/TMD) form was used to diagnose TMD. The relationships between TMD/RDC clinical diagnoses and psychoemotional status, as described by the Beck's Depression Inventory (BDI) and Perceived Stress Scale (PSS-10), were analyzed. We divide the group into four on the basis of RDC/TMD Axis I diagnosis. Group 0 included 30 students lacking TMD symptoms. Group I consisted of 30 people with myofascial pain (group IA in RDC/TMD). Group II contained 23 people with disk displacement with reduction (group IIA in RDC/TMD). Group III contained ten people (Group III diagnosis, often associated with pain). RESULTS: We did not find statistically significant differences between the study groups. In subjects with pain (Groups I and III), we found the mean value on the BDI and PSS-10 scales to be higher than among the pain-free subjects (Groups 0 and II). CONCLUSION: In young adults with TMD accompanied by pain, psychoemotional status should also be evaluated.
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Artralgia/psicologia , Artrite/psicologia , Depressão/psicologia , Luxações Articulares/psicologia , Estresse Psicológico/psicologia , Disco da Articulação Temporomandibular , Síndrome da Disfunção da Articulação Temporomandibular/psicologia , Adolescente , Estudos de Casos e Controles , Feminino , Humanos , Masculino , Dor/psicologia , Polônia , Escalas de Graduação Psiquiátrica , Estudantes , Transtornos da Articulação Temporomandibular/psicologia , Adulto JovemRESUMO
Headache is a common problem in the population, which decreases the quality of life and makes everyday functioning difficult. It often coexists with typical symptoms of temporomandibular disorders. The objective of the study was to clarify whether there is a relationship between the presence of headache in young volunteers and the mastication muscle tone. Material and Method. Volunteers aged 18 years who underwent general dental examination, clinical evaluation, and examination using the dual-axis diagnostic system Research Diagnostic Criteria for Temporomandibular Disorders (RDC/TMD) form in the Polish language version participated in the study. On the basis of the examination results, these individuals were divided into three groups according to RDC results. A group of healthy individuals (axis I value 0), a group of sick individuals (axis I diagnosis Ia, IIa, or IIIa), and a group of individuals suffering from depression (axis II values 1-3) were singled out. Then, volunteers were divided into two groups: group I reporting headache and group II without any pain. In each of these individuals, the contractile activity of the masseter muscles and anterior temporal muscles was recorded bilaterally as the surface electromyographic activity (sEMG) at rest, during swallowing, and maximum clenching. The activity index ranging between +100 (activity of the masseter muscles only) and -100 (activity of the anterior temporal muscles only) was used to indicate the predominance of activity of the study muscles. Results. The statistical analysis of data showed that there was a significant relationship between the presence of headache and a change in the mastication muscle tone, expressed as the predominant activity of the temporal muscles, only in the group of sick individuals during maximum clenching. Summary. The diversity of sEMG results implies that a change in the mastication muscle tone is not a direct consequence of headache, but it has to be modified by other factors.
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Cefaleia/patologia , Músculo Masseter/fisiopatologia , Tono Muscular/fisiologia , Adolescente , Eletromiografia , Feminino , Humanos , Masculino , Mastigação/fisiologiaRESUMO
Genetic evidence for a link between DNA replication and glycolysis has been demonstrated a decade ago in Bacillus subtilis, where temperature-sensitive mutations in genes coding for replication proteins could be suppressed by mutations in genes of glycolytic enzymes. Then, a strong influence of dysfunctions of particular enzymes from the central carbon metabolism (CCM) on DNA replication and repair in Escherichia coli was reported. Therefore, we asked if such a link occurs only in bacteria or it is a more general phenomenon. Here, we demonstrate that effects of silencing (provoked by siRNA) of expression of genes coding for proteins involved in DNA replication and repair (primase, DNA polymerase ι, ligase IV, and topoisomerase IIIß) on these processes (less efficient entry into the S phase of the cell cycle and decreased level of DNA synthesis) could be suppressed by silencing of specific genes of enzymes from CMM. Silencing of other pairs of replication/repair and CMM genes resulted in enhancement of the negative effects of lower expression levels of replication/repair genes. We suggest that these results may be proposed as a genetic evidence for the link between DNA replication/repair and CMM in human cells, indicating that it is a common biological phenomenon, occurring from bacteria to humans.
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Carbono/metabolismo , Reparo do DNA , Replicação do DNA , Fibroblastos/metabolismo , Genes Bacterianos/fisiologia , Ciclo Celular/genética , Divisão Celular/genética , Reparo do DNA/genética , Replicação do DNA/genética , Escherichia coli/genética , Escherichia coli/metabolismo , Regulação Bacteriana da Expressão Gênica , Inativação Gênica/fisiologia , Humanos , Redes e Vias Metabólicas/genética , Organismos Geneticamente ModificadosRESUMO
Previous reports and our recently published data indicated that some enzymes of glycolysis and the tricarboxylic acid cycle can affect the genome replication process by changing either the efficiency or timing of DNA synthesis in human normal cells. Both these pathways are connected with the pentose phosphate pathway (PPP pathway). The PPP pathway supports cell growth by generating energy and precursors for nucleotides and amino acids. Therefore, we asked if silencing of genes coding for enzymes involved in the pentose phosphate pathway may also affect the control of DNA replication in human fibroblasts. Particular genes coding for PPP pathway enzymes were partially silenced with specific siRNAs. Such cells remained viable. We found that silencing of the H6PD, PRPS1, RPE genes caused less efficient enterance to the S phase and decrease in efficiency of DNA synthesis. On the other hand, in cells treated with siRNA against G6PD, RBKS and TALDO genes, the fraction of cells entering the S phase was increased. However, only in the case of G6PD and TALDO, the ratio of BrdU incorporation to DNA was significantly changed. The presented results together with our previously published studies illustrate the complexity of the influence of genes coding for central carbon metabolism on the control of DNA replication in human fibroblasts, and indicate which of them are especially important in this process.
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Replicação do DNA/genética , DNA/biossíntese , Glucosefosfato Desidrogenase/genética , Transaldolase/genética , Proliferação de Células/genética , Ciclo do Ácido Cítrico/genética , DNA/genética , Fibroblastos/metabolismo , Glicólise/genética , Humanos , Via de Pentose Fosfato/genética , RNA Interferente PequenoRESUMO
of this study was to analyze the effects of carbon dioxide (CO2) laser light on the structure and elemental composition of dentine. METHODS: The evaluation was conducted on samples from extracted teeth. The surface of the dentine was exposed to the radiation from a CTL 1401 CO2 laser (Centre of Laser Technology, Poland). The radiation and frequency parameters were as follows: group I with 5 W and 1 Hz, group II with 10 W and 1 Hz, group III with 5 W and 5 Hz, and group IV with 10 W and 5 Hz. The altered dentine structure was macroscopically and microscopically evaluated using a Nova NanoSEM 200 Scanning Electron Microscope (FEI Europe) with integrated microanalysis X-ray system for elemental analysis in points. RESULTS: There were significant differences between groups in the macro- and microstructure of laser defects. CONCLUSIONS: CO2 laser radiation causes irreversible, destructive changes in dentine. The structural dentine lesions developed under the influence of the CO2 laser radiation may hinder proper adhesion of bonding systems with the damaged tissue. Laser defects in the structure should be treated like defects of noncarious origin requiring preparation and filling with composite materials in accordance with the procedures.
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Dentina/química , Dentina/ultraestrutura , Lasers de Gás , Dente Molar/química , Dente Molar/ultraestrutura , Dentina/efeitos da radiação , Relação Dose-Resposta à Radiação , Técnicas In Vitro , Dente Molar/efeitos da radiação , Doses de RadiaçãoRESUMO
AIM: Denture-related stomatitis is a disorder that often affects denture wearers. The purpose of this study was to evaluate the intensity, genera, and frequency of yeasts in the oral cavity of complete denture wearers in terms of subject gender and age. MATERIALS AND METHODS: Nine hundred twenty patients (307 males and 613 females) with complete upper dentures were selected for the study and divided into four age groups: ≤50 years, 51-60, 61-70, and >70 years. Yeast samples were taken as a smear from the palate. The data were collected from January 15, 2007 to January 15, 2012. RESULTS: The distribution of the number of yeast colonies by gender was statistically significant (P=0.02). Across all subjects, there was a statistically significant relationship between the intensity of yeast growth and the gender (P=0.01). In every age group, the number of infection-free individuals was greater among males than females. Intermediate, intense, and abundant growth of yeast occurred most frequently in the youngest group of females. CONCLUSION: The genera of Candida species and the frequency of yeast infection in denture wearers appear to be influenced by both age and gender. The complete denture wearers ≤50 years of age appeared to have the greatest proclivity to oral Candida infections.
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Fatores Etários , Candida/isolamento & purificação , Prótese Total/microbiologia , Boca/microbiologia , Fatores Sexuais , Estomatite sob Prótese/epidemiologia , Adulto , Idoso , Idoso de 80 Anos ou mais , Candida/classificação , Feminino , Humanos , Masculino , Pessoa de Meia-Idade , Polônia/epidemiologia , Estudos RetrospectivosRESUMO
BACKGROUND: This study reports the cephalometric evaluation of a group of adolescent Polish individuals describing dento-facial structure as well as details of incisor position and soft tissue characteristics. The results should reveal morphological features specific to Polish persons and serve as a comparative material for future diagnostic procedures. METHODS: The study was based on an analysis of cephalgrams of 122 Polish adolescents average age 18 years 6 months analysed in a computer system using the Kracovia composite system analysis describing dento-facial morphology ad modum Björk as well as soft tissue factors. The control material was based on published reports by Björk (Dento-facial characteristics) Riketts and Holdaway (soft tissue profile). RESULTS: The comparative study revealed a slight reduction in the sagittal jaw relationship with a significant reduction in the vertical jaw relationship and a distinctive mandibular morphology with a reduced jaw angle and an increase in the "Beta angle". These findings were reflected in the soft tissue pattern. The soft tissue profile reflected the skeletal cephalometrics observation. CONCLUSION: The dento-facial profile of Polish adolescents demonstrates specific characteristics which should be taken into account when diagnosing facial form in connection with orthodontic treatment planning in particular Polish patients.